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1. Arzate H, Alvarez MA, Narayanan AS: Cyclosporin A promotes mineralization by human cementoblastoma-derived cells in culture. J Periodontal Res; 2005 Jun;40(3):218-24
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Cyclosporin A promotes mineralization by human cementoblastoma-derived cells in culture.
  • OBJECTIVE: The immunosuppressive drug cyclosporin A has been shown to induce cementum deposition in vivo in experimental animals.
  • Using cementoblastoma-derived cells, we have studied whether this drug will be useful to study cementum mineralization and differentiation in vitro.
  • METHODS: Human cementoblastoma cells and gingival fibroblasts (controls) were cultured and treated with 0.5, 1.0 and 5.0 microg/ml of cyclosporin A.
  • RESULTS: Cyclosporin A at 5.0 microg/ml concentration reduced significantly the increase in the number of cementoblastoma cells.
  • A dose-dependent increase in the number of mineralized nodules occurred in cultures of cementoblastoma-derived cells treated with cyclosporin A, and RT-PCR analyses showed significantly higher levels of expression of alkaline phosphatase, bone sialoprotein, type I collagen, matrix metalloproteinase-1, osteocalcin, osteopontin, and Cbfa1.
  • Mineralized nodules were not detected in gingival fibroblasts and bone specific proteins were not expressed.
  • CONCLUSIONS: Presence of cyclosporin A during 14-day culture period appears to suppress the proliferation of cementoblastoma cells and induce the formation mineralized-like tissue by these cells.
  • [MeSH-major] Calcification, Physiologic / drug effects. Cyclosporine / pharmacology. Dental Cementum / drug effects. Immunosuppressive Agents / pharmacology
  • [MeSH-minor] Cell Proliferation / drug effects. Humans. Reverse Transcriptase Polymerase Chain Reaction

  • Hazardous Substances Data Bank. CYCLOSPORIN A .
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  • (PMID = 15853967.001).
  • [ISSN] 0022-3484
  • [Journal-full-title] Journal of periodontal research
  • [ISO-abbreviation] J. Periodont. Res.
  • [Language] eng
  • [Grant] United States / NIDCR NIH HHS / DE / DE-013061
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] Denmark
  • [Chemical-registry-number] 0 / Immunosuppressive Agents; 83HN0GTJ6D / Cyclosporine
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