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1. Jiménez-Heffernan A, Gómez-Millán J, Sánchez De Mora E, Delgado Moreno J, Delgado Gil MM, Salgado C, Contreras Puertas P, Bermúdez Morales MC, López J, Paz Expósito J: [Quantitative salivary gland scintigraphy in head and neck cancer patients following radiotherapy]. Rev Esp Med Nucl; 2010 Jul-Aug;29(4):165-71
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  • OBJECTIVE: To assess the role of quantitative salivary gland scintigraphy (SGS) in the detection of functional impairment of salivary glands in patients with head and neck cancer treated with radiotherapy (RT).
  • Three studies were performed to each patient: baseline, 3 and 18 months after RT.
  • Excretion fraction (EF), counts per minute/pixel/MBq (CMPM) and uptake percentage were obtained from time-activity curves from ROIs placed over parotid (PG) and submandibular glands (SMG) and related to the doses received by the PG.
  • CONCLUSIONS: EF was more sensitive than uptake in assessing post-RT impairment of salivary function.
  • In addition, it reflected functional recovery of parotid glands over time.

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  • [Copyright] Copyright © 2009 Elsevier España, S.L. y SEMNIM. All rights reserved.
  • (PMID = 20462671.001).
  • [ISSN] 0212-6982
  • [Journal-full-title] Revista española de medicina nuclear
  • [ISO-abbreviation] Rev Esp Med Nucl
  • [Language] spa
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Spain
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2. Sun JF, Zhao DD, Han XP, Jin HS, Yu L: [Dynamic detection of chimerism and fusion gene in chronic myeloid leukemia patients relapsed after allogeneic hematopoietic stem cell transplantation]. Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2008 Aug;16(4):833-7
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  • This study was aimed to investigate the chimerism and fusion gene expression in patients with CML after allo-HSCT, to analyse engraftment and minimal residual disease by using STR-PCR combined with RT-PCR qualitative and quantitative assays, and to evaluate their clinical value for predicting disease relapse.
  • Qualitative analysis of donor chimerism was performed by multiplex PCR amplification of STR markers and capillary electrophoresis with fluorescence detection, qualitative detection of bcr/abl transcripts was performed by RT-PCR.
  • The results showed that the 100% donor chimerism appeared in 4 patients on day 28 after transplantation and bcr/abl expression was negative, but the 4 patients were in status of unstable mixed chimerism (DC: 0% - 80.4%) at the different time points during the following up with bcr/abl gene positive.
  • It is concluded that the results of STR-PCR in the range of its sensitivity fully correspond with bcr/abl tests in patients.
  • The combination of STR-PCR with RT-PCR will provide a highly sensitive and valuable tool for evaluating engraftment, graft rejection, and relapse and predicting GVHD.


3. Nuyts S, Dirix P, Clement PM, Poorten VV, Delaere P, Schoenaers J, Hermans R, Van den Bogaert W: Impact of adding concomitant chemotherapy to hyperfractionated accelerated radiotherapy for advanced head-and-neck squamous cell carcinoma. Int J Radiat Oncol Biol Phys; 2009 Mar 15;73(4):1088-95
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  • PURPOSE: To evaluate the feasibility and efficacy of a hyperfractionated accelerated radiotherapy (RT) schedule combined with concomitant chemotherapy (Cx) in patients with locally advanced head-and-neck squamous cell carcinoma.
  • METHODS AND MATERIALS: Between 2004 and 2007, a total of 90 patients with locoregionally advanced head-and-neck squamous cell carcinoma underwent irradiation according to a hybrid fractionation schedule consisting of 20 fractions of 2 Gy (once daily) followed by 20 fractions of 1.6 Gy (twice daily) to a total dose of 72 Gy.
  • In comparison with the RT-only group, an improvement of 15% in both LRC (p = 0.03) and overall survival (p = 0.09) was observed.
  • No acute Grade 4 or 5 toxicity was seen, but incidences of Grade 3 acute mucositis (74.5% vs. 50.7%; p = 0.002) and dysphagia (82.2% vs. 47.9%; p < 0.001) were significantly higher in the chemoradiotherapy group compared with patients treated with RT alone.


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4. Nayak AK, Rose JB: Detection of Helicobacter pylori in sewage and water using a new quantitative PCR method with SYBR green. J Appl Microbiol; 2007 Nov;103(5):1931-41
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  • AIMS: To demonstrate the application of a new quantitative polymerase chain reaction (qPCR) technique for the determination of Helicobacter pylori concentrations in water, and to use this method to investigate the occurrence of the bacteria in sewage.
  • METHODS AND RESULTS: The detection of H. pylori in water was aided by PCR using specific primers designed for the amplification of a fragment within the major vacuolating cytotoxin gene.
  • Conventional culture was compared with conventional PCR and the new real-time (RT) qPCR approach for the quantification of the bacterium.
  • RT qPCR demonstrated a 100-fold greater sensitivity for the detection of H. pylori DNA in comparison with conventional PCR.
  • Helicobacter pylori was found at 2-28 cells ml(-1) in sewage, of the 23 sewage samples - 84% were positive for H. pylori species-specific vacuolating cyctotoxin gene (vacA) by RT qPCR, but were negative by conventional PCR.
  • CONCLUSIONS: The RT qPCR assay provided a specific, sensitive and rapid method for the quantitative detection of H. pylori in sewage.
  • SIGNIFICANCE AND IMPACT OF THE STUDY: The quantitative detection of H. pylori by rapid and less-expensive methods than the TaqMan Assay using SYBR green could be an important tool to monitor infection in community by measuring the concentrations in sewage and to meet the new regulatory and risk-based frameworks for water supplies.

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  • (PMID = 17953603.001).
  • [ISSN] 1364-5072
  • [Journal-full-title] Journal of applied microbiology
  • [ISO-abbreviation] J. Appl. Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA Primers; 0 / Fluorescent Dyes; 0 / Organic Chemicals; 0 / Sewage; 163795-75-3 / SYBR Green I
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5. Delaporte V, Muracciole X, Lechevallier E, Bastide C, Cowen D, Rossi D, Coulange C: [Prostate cancer at high risk of recurrence: results of 12 months of radiotherapy-hormone therapy]. Prog Urol; 2005 Apr;15(2):260-4
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  • [Transliterated title] Cancer de la prostate à haut risque de rechute. Résultats d'une RT-HT de 12 mois.
  • [MeSH-minor] Disease-Free Survival. Follow-Up Studies. Humans. Male. Neoplasm Recurrence, Local / epidemiology. Risk Factors

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  • (PMID = 15999604.001).
  • [ISSN] 1166-7087
  • [Journal-full-title] Progrès en urologie : journal de l'Association française d'urologie et de la Société française d'urologie
  • [ISO-abbreviation] Prog. Urol.
  • [Language] fre
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] France
  • [Chemical-registry-number] 0 / Androgen Antagonists; 33515-09-2 / Gonadotropin-Releasing Hormone
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6. Reuter RR, Carroll JA, Dailey JW, Cook BJ, Galyean ML: Effects of dietary energy source and level and injection of tilmicosin phosphate on immune function in lipopolysaccharide-challenged beef steers. J Anim Sci; 2008 Aug;86(8):1963-76
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  • Increased energy intake (70AL) increased (P < or = 0.04) DMI, ADG, and rectal temperature (RT) after the challenge compared with the 70RES treatment.
  • The 30AL treatment increased the maximum concentrations and area under the response curve of the proinflammatory cytokines (PIC) interferon-gamma, tumor necrosis factor-alpha, and IL-6 (P < or = 0.05) compared with the average of the 70AL and 70RES treatments.
  • Decreased energy intake (70RES vs. 70AL) increased IL-6 (P < or = 0.003) but did not significantly increase interferon-gamma and tumor necrosis factor-alpha (P > or = 0.14) after LPS administration.
  • Tilmicosin administration decreased the time to attain maximal RT (P = 0.01) by 1 h without altering the peak RT (P = 0.85), and tilmicosin interacted with energy intake to increase prechallenge PIC in 70RES vs. 70AL (P < or = 0.05).
  • [MeSH-minor] Acute-Phase Proteins / metabolism. Animal Nutritional Physiological Phenomena. Animals. Cytokines / blood. Diet / veterinary. Hydrocortisone / blood. Male. Time Factors

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  • (PMID = 18407986.001).
  • [ISSN] 1525-3163
  • [Journal-full-title] Journal of animal science
  • [ISO-abbreviation] J. Anim. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Acute-Phase Proteins; 0 / Anti-Bacterial Agents; 0 / Cytokines; 0 / Lipopolysaccharides; WI4X0X7BPJ / Hydrocortisone; XL4103X2E3 / tilmicosin; YEF4JXN031 / Tylosin
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7. Jeong WJ, Jung EJ, Hah JH, Kwon TK, Wu HG, Heo DS, Sung MW, Kim KH: Preliminary results of pre-radiation neck dissection in head and neck cancer patients undergoing organ preservation treatment. Acta Otolaryngol Suppl; 2007 Oct;(558):121-7
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  • CONCLUSION: Pre-RT ND in patients with HNSCC undergoing organ preservation treatment is safe, advantageous, poses no additional morbidity owing to the elective neck dissection, and may possibly improve survival outcomes.
  • OBJECTIVE: Establish the role of pre-radiation neck dissection (pre-RT ND) in patients with head & neck squamous cell carcinoma (HNSCC) undergoing organ preservation treatment.
  • MATERIALS AND METHODS: Fourteen patients with histologically confirmed HNSCC in stages III approximately IV with proven regional metastasis were enrolled in the organ preservation approach incorporating pre-RT ND at a tertiary referral center between May 1998 and August 2004.
  • RESULTS: Disease free survival was significantly better for the pre-RT ND group.
  • There was no significant difference in overall survival, pattern of recurrence, and primary organ preservation rate between the two groups.
  • No significant morbidity owing to neck dissection was noted in patients who underwent neck dissection.
  • Although the delivery of radiation to the primary site was delayed for patients in the pre-RT ND group, it did not influence the major outcomes.
  • [MeSH-minor] Antineoplastic Combined Chemotherapy Protocols / therapeutic use. Bridged Compounds / administration & dosage. Chemotherapy, Adjuvant. Cisplatin / administration & dosage. Disease-Free Survival. Female. Fluorouracil / administration & dosage. Humans. Male. Middle Aged. Neoplasm Metastasis. Neoplasm Recurrence, Local / epidemiology. Radiotherapy Dosage. Taxoids / administration & dosage

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  • (PMID = 17882582.001).
  • [ISSN] 0365-5237
  • [Journal-full-title] Acta oto-laryngologica. Supplementum
  • [ISO-abbreviation] Acta Otolaryngol Suppl
  • [Language] eng
  • [Publication-type] Controlled Clinical Trial; Journal Article
  • [Publication-country] Norway
  • [Chemical-registry-number] 0 / Bridged Compounds; 0 / Taxoids; 1605-68-1 / taxane; Q20Q21Q62J / Cisplatin; U3P01618RT / Fluorouracil
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8. Tan IL, Dimamay MP, Buerano CC, Alfon JA, Tanig CZ, Matias RR, Natividad FF: Development and evaluation of a fluorogenic real-time RT-PCR for the detection of dengue 3 virus. J Med Virol; 2010 Dec;82(12):2053-63
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  • [Title] Development and evaluation of a fluorogenic real-time RT-PCR for the detection of dengue 3 virus.
  • A dengue-3-specific real-time reverse transcriptase-polymerase chain reaction (RT-PCR) was developed using the novel Light Upon eXtension (LUX™) fluorogenic technology.
  • Moreover, the fluorogenic assay was evaluated by using clinical specimens and comparing test results with historical data obtained from conventional RT-PCR, which served as the criterion standard.
  • Using patient sera as test samples, the assay demonstrated 95.45% sensitivity and 100% specificity, respectively.
  • These results reveal that the real-time RT-PCR assay may be utilized as a rapid, convenient, and sensitive tool for the detection of dengue 3 in clinical and laboratory specimens.

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  • (PMID = 20981793.001).
  • [ISSN] 1096-9071
  • [Journal-full-title] Journal of medical virology
  • [ISO-abbreviation] J. Med. Virol.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Fluorescent Dyes; 0 / NS1 protein, Dengue virus type 3; 0 / Oligonucleotide Probes; 0 / RNA, Viral; 0 / Viral Nonstructural Proteins
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9. Su AW, Jani AB: Chronic genitourinary and gastrointestinal toxicity of prostate cancer patients undergoing pelvic radiotherapy with intensity-modulated versus 4-field technique. Am J Clin Oncol; 2007 Jun;30(3):215-9
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  • METHODS: The records of consecutive prostate cancer patients receiving RT at a single institution with a minimum follow-up of 120 days were reviewed; 48 of these patients received a prostate boost preceded by pelvic radiotherapy (PRT), 14 with IMRT (IM-PRT), and 34 with 4-field (4F-PRT).
  • Dosimetric endpoints for the bladder, rectum, composite, and target for the PRT plans were compared using the 2-tailed t test.
  • Late RTOG GU and GI toxicity were compared using the chi test.

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  • [CommentIn] Am J Clin Oncol. 2007 Dec;30(6):666 [18091067.001]
  • (PMID = 17551295.001).
  • [ISSN] 1537-453X
  • [Journal-full-title] American journal of clinical oncology
  • [ISO-abbreviation] Am. J. Clin. Oncol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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10. Wisnewski RG, Lauwereyns J: Systemic dizocilpine (MK-801) facilitates performance in opposition to response bias. Behav Brain Funct; 2007;3:48
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  • The current study examined the effects of the noncompetitive N-methyl-D-aspartate (NMDA)-receptor antagonist dizocilpine (MK-801) on reaction time (RT) in a nose-poke task with rats completing an asymmetric reward schedule.
  • Reaction times (ms) were faster for large-reward trials than for small-reward trials, replicating previous findings.
  • At a dosage of MK-801 (0.04 mg/kg), there was no significant influence of on RT in large-reward trials.
  • In contrast, the same dosage of MK-801 in small-reward trials produced a decrease in RT as compared to the control condition, implying an improvement of performance.
  • Below 0.04 mg/kg of MK-801, a steady decrease of RT in small-trials was seen as a function of dosage.
  • Above 0.04 mg/kg of MK-801, the majority of rats failed to perform the task at all, whereas the rats that did manage to perform the criterion of 80 correct trials in a session showed no difference in RT between large- and small-reward trials.
  • These data indicate that the systemic administration of a relatively small dosage of MK-801 facilitates performance when reward is small.

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  • [ISSN] 1744-9081
  • [Journal-full-title] Behavioral and brain functions : BBF
  • [ISO-abbreviation] Behav Brain Funct
  • [Language] eng
  • [Publication-type] Journal Article
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  • [Other-IDs] NLM/ PMC2075506
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11. Mattson CL, Campbell RT, Karabatsos G, Agot K, Ndinya-Achola JO, Moses S, Bailey RC: Scaling sexual behavior or "sexual risk propensity" among men at risk for HIV in Kisumu, Kenya. AIDS Behav; 2010 Feb;14(1):162-72
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  • We found no evidence of differential item function at different time points (except for reporting a concurrent partners when comparing 6 and 12 month follow-up visits) or with respect to the language with which the instrument was administered.

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  • (PMID = 18651213.001).
  • [ISSN] 1573-3254
  • [Journal-full-title] AIDS and behavior
  • [ISO-abbreviation] AIDS Behav
  • [Language] eng
  • [Grant] United States / NIAID NIH HHS / AI / U01 AI050440; United States / NIAID NIH HHS / AI / U01 AI050440-08
  • [Publication-type] Journal Article; Randomized Controlled Trial
  • [Publication-country] United States
  • [Other-IDs] NLM/ NIHMS83247; NLM/ PMC2999988
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12. Corey DM, Kim HW, Salazar R, Illescas R, Villena J, Gutierrez L, Sanchez J, Tabet SR: Brief report: effectiveness of combination antiretroviral therapy on survival and opportunistic infections in a developing world setting: an observational cohort study. J Acquir Immune Defic Syndr; 2007 Apr 1;44(4):451-5
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  • All 564 initially antiretroviral-naive HIV-infected persons (mean CD4 count of 91 cells/mm3) who received combination ART were followed over time.
  • Among persons who initiated therapy with CD4 counts <100 cells/mm3, the overall survival rate at 3 years was 95%.
  • Patients who received 2 reverse transcriptase (RT) inhibitors plus a protease inhibitor had slightly better survival rates and less opportunistic disease in the first year of therapy as compared with those receiving 2 RT inhibitors and a nonnucleoside reverse transcriptase inhibitor or 3 RT inhibitors.


13. Hoshi H, O'Brien J, Mills SL: A novel fluorescent tracer for visualizing coupled cells in neural circuits of living tissue. J Histochem Cytochem; 2006 Oct;54(10):1169-76
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  • Staining the coupled cells by this means opens the door to many vital techniques including paired-cell electrophysiology, RT-PCR, and morphological characterization of previously unknown coupled cells.
  • Tracers commonly used at the present time are not generally suitable for these purposes in many tissues, including neurons.

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  • (PMID = 16864895.001).
  • [ISSN] 0022-1554
  • [Journal-full-title] The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
  • [ISO-abbreviation] J. Histochem. Cytochem.
  • [Language] ENG
  • [Grant] United States / NEI NIH HHS / EY / EY-10608; United States / NEI NIH HHS / EY / EY-12857; United States / NEI NIH HHS / EY / EY012857-06A1; United States / NEI NIH HHS / EY / R01 EY010121; United States / NEI NIH HHS / EY / R01 EY012857; United States / NEI NIH HHS / EY / EY-10121; United States / NEI NIH HHS / EY / R01 EY012857-06A1; United States / NEI NIH HHS / EY / P30 EY010608
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Benzoxazoles; 0 / Connexins; 0 / Eye Proteins; 0 / Fluorescent Dyes; 0 / Po-Pro-1; 0 / connexin 35 protein, vertebrate
  • [Other-IDs] NLM/ NIHMS17898; NLM/ PMC1851887
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14. Gamer AO, Rossbacher R, Kaufmann W, van Ravenzwaay B: The inhalation toxicity of di- and triethanolamine upon repeated exposure. Food Chem Toxicol; 2008 Jun;46(6):2173-83
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  • Systemic and respiratory tract (RT) toxicity of triethanolamine (TEA) was assessed in a 28-day nose-only inhalation study in Wistar rats (10animals/sex, concentrations: 0, 20, 100, 500mg/m3; 5 days/week, 6h/day).
  • TEA appears to be less potent with respect to systemic toxicity and RT irritancy than DEA.
  • [MeSH-minor] Animals. Blood Cell Count. Epithelium / pathology. Erythrocyte Count. Female. Inhalation Exposure. Irritants / toxicity. Larynx / pathology. Male. Neurotoxicity Syndromes / psychology. No-Observed-Adverse-Effect Level. Rats. Rats, Wistar. Respiratory System / pathology. Urinalysis

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  • (PMID = 18420328.001).
  • [ISSN] 0278-6915
  • [Journal-full-title] Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • [ISO-abbreviation] Food Chem. Toxicol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Ethanolamines; 0 / Irritants; 9O3K93S3TK / triethanolamine; AZE05TDV2V / diethanolamine
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15. Bethel CR, Bieberich CJ: Loss of Nkx3.1 expression in the transgenic adenocarcinoma of mouse prostate model. Prostate; 2007 Dec 1;67(16):1740-50
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  • Quantitative RT-PCR analyses were performed to determine relative mRNA expression.
  • Poorly differentiated (PD) tumors stained negatively for Nkx3.1.
  • Quantitative RT-PCR analyses revealed the presence of Nkx3.1 mRNA in each lobe at all ages, albeit reduced to variable levels.

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  • (PMID = 17929276.001).
  • [ISSN] 0270-4137
  • [Journal-full-title] The Prostate
  • [ISO-abbreviation] Prostate
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / CA94818-04
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Homeodomain Proteins; 0 / Nkx3-1 protein, mouse; 0 / RNA, Messenger; 0 / Receptors, Androgen; 0 / Transcription Factors
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16. Bergero R, Lanfranco L, Ghignone S, Bonfante P: Enhanced activity of the GmarMT1 promoter from the mycorrhizal fungus Gigaspora margarita at limited carbon supply. Fungal Genet Biol; 2007 Sep;44(9):877-85
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  • We previously reported the identification and the characterization of a metallothionein gene (GmarMT1) from the arbuscular mycorrhizal fungus Gigaspora margarita.
  • Here, we have used real-time quantitative RT-PCR to show that GmarMT1 expression was turned off during the symbiotic fungal growth in the hexose-rich mycorrhizal apoplast, whereas transcripts were abundant during all other fungal growth stages, when products of lipid breakdown and the glyoxylate cycle feed carbohydrate-consuming pathways.
  • In support of a nutritional regulation of GmarMT1 expression, we show that transcriptional activity of GmarMT1 promoter in yeast was strongly induced by glucose starvation (up to 20 times the basal level).

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  • (PMID = 17482490.001).
  • [ISSN] 1087-1845
  • [Journal-full-title] Fungal genetics and biology : FG & B
  • [ISO-abbreviation] Fungal Genet. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 7440-44-0 / Carbon; 9038-94-2 / Metallothionein
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17. Barber M, Murrell A, Ito Y, Maia AT, Hyland S, Oliveira C, Save V, Carneiro F, Paterson AL, Grehan N, Dwerryhouse S, Lao-Sirieix P, Caldas C, Fitzgerald RC: Mechanisms and sequelae of E-cadherin silencing in hereditary diffuse gastric cancer. J Pathol; 2008 Nov;216(3):295-306
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  • Archival tumour material was analysed from 16 patients with CDH1 germline mutations and seven patients fulfilling HDGC criteria without CDH1 germline mutations.
  • Cadherin expression levels were determined by real-time RT-PCR and immunohistochemistry.
  • Tumours from 4/16 individuals had promoter hypermethylation that was restricted to the A allele haplotype in three cases.
  • E-cadherin loss (mRNA and protein) generally correlated with identification of a second hit.


18. Nguyen PL, D'Amico AV, Lee AK, Suh WW: Patient selection, cancer control, and complications after salvage local therapy for postradiation prostate-specific antigen failure: a systematic review of the literature. Cancer; 2007 Oct 1;110(7):1417-28
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  • Among men who experience prostate-specific antigen (PSA) failure after external beam radiation or brachytherapy (RT), many will harbor occult micrometastases; however, a significant minority will have a true local-only failure and, thus, potentially may benefit from a salvage local therapy.
  • Those most likely to have a local-only failure initially have low-risk disease (PSA < 10 ng/mL, Gleason score < or =6, clinical T1c or T2a tumor status), pretreatment PSA velocity < 2.0 ng/mL per year at the time of initial presentation, interval to PSA failure > 3 years, PSA doubling time > 12 months, negative bone scan and pelvic imaging, and positive rebiopsy.
  • In addition, men with presalvage PSA levels > 10 ng/mL, presalvage T3/T4 disease, or presalvage Gleason scores > or =7 on a rebiopsy sample without significant RT effects are unlikely to be cured by salvage local therapy.
  • Based on a review of all series of post-RT salvage prostatectomy, cryosurgery, and brachytherapy published in English since 1990, morbidity can be substantial.
  • Although urinary incontinence appeared to be greater after salvage prostatectomy (41%) or cryosurgery (36%) than after brachytherapy (6%), patients who received salvage brachytherapy faced a 17% risk of grade 3 or 4 genitourinary complications and a fistula risk that averaged 3.4% across all series.
  • [MeSH-major] Neoplasm Recurrence, Local / therapy. Patient Selection. Prostate-Specific Antigen / blood. Prostatic Neoplasms / immunology. Prostatic Neoplasms / therapy. Salvage Therapy / methods


19. Fu G, Liu M, Zeng W, Pu J, Bi Y, Ma G, Liu J: Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses. Virus Genes; 2010 Oct;41(2):236-40
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  • [Title] Establishment of a multiplex RT-PCR assay to detect different lineages of swine H1 and H3 influenza A viruses.
  • The reverse transcriptase polymerase chain reaction (RT-PCR) assay is an effective method for use in influenza surveillance.
  • In this study, a multiplex RT-PCR method was developed for simultaneous identification of hemagglutinin (HA) genes derived from the three lineages of swine influenza viruses.
  • This multiplex RT-PCR method provides a rapid and specific swine influenza diagnostic tool that also has the potential for investigating the epidemiology of different lineages of swine influenza virus prevalent currently in China.

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  • (PMID = 20700759.001).
  • [ISSN] 1572-994X
  • [Journal-full-title] Virus genes
  • [ISO-abbreviation] Virus Genes
  • [Language] eng
  • [Publication-type] Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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20. Schaue D, Comin-Anduix B, Ribas A, Zhang L, Goodglick L, Sayre JW, Debucquoy A, Haustermans K, McBride WH: T-cell responses to survivin in cancer patients undergoing radiation therapy. Clin Cancer Res; 2008 Aug 1;14(15):4883-90
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  • PURPOSE: The goal of this study was to determine if radiation therapy (RT) of human cancer enhances or diminishes tumor-specific T-cell reactivity.
  • Similar samples were taken from prostate cancer patients receiving standard RT.
  • The level of CD8(+) T cells capable of binding tetramers for the tumor-associated antigen survivin, which is overexpressed in both cancer types, was enumerated in HLA-A*0201 patient samples.
  • Increases were more common in CRC patients whose tumor was downstaged after chemoradiotherapy.
  • CONCLUSION: This study indicates that RT may increase the likelihood of some cancer patients responding to immunotherapy and lays a basis for future investigations aimed at combining radiation and immunotherapy.

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  • (PMID = 18676762.001).
  • [ISSN] 1078-0432
  • [Journal-full-title] Clinical cancer research : an official journal of the American Association for Cancer Research
  • [ISO-abbreviation] Clin. Cancer Res.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / CA101752-04; United States / NCI NIH HHS / CA / R01 CA101752; United States / NCI NIH HHS / CA / R01 CA-101752; United States / NCI NIH HHS / CA / R01 CA101752-04
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / BIRC5 protein, human; 0 / FOXP3 protein, human; 0 / Forkhead Transcription Factors; 0 / HLA-A Antigens; 0 / HLA-A*02:01 antigen; 0 / HLA-A2 Antigen; 0 / Inhibitor of Apoptosis Proteins; 0 / Interleukin-2 Receptor alpha Subunit; 0 / Microtubule-Associated Proteins; 0 / Neoplasm Proteins
  • [Other-IDs] NLM/ NIHMS96056; NLM/ PMC2748652
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21. Seddas PM, Arnould C, Tollot M, Arias CM, Gianinazzi-Pearson V: Spatial monitoring of gene activity in extraradical and intraradical developmental stages of arbuscular mycorrhizal fungi by direct fluorescent in situ RT-PCR. Fungal Genet Biol; 2008 Aug;45(8):1155-65
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  • [Title] Spatial monitoring of gene activity in extraradical and intraradical developmental stages of arbuscular mycorrhizal fungi by direct fluorescent in situ RT-PCR.
  • To overcome this drawback, a direct fluorescent in situ RT-PCR methodology was developed for spatial mapping of gene expression in different presymbiotic and symbiotic structures of an arbuscular mycorrhizal fungus.
  • Transcript detection was optimized by targeting the LSU rRNA gene of Glomus intraradices and monitoring expression of a stearoyl-CoA-desaturase gene that is consistently expressed at high levels in spores, hyphae, arbuscules and vesicles.
  • Direct fluorescent in situ RT-PCR offers new perspectives for the sensitive analysis of fungal developmental processes that occur during functional differentiation in symbiotic arbuscular mycorrhiza interactions.

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  • (PMID = 18585067.001).
  • [ISSN] 1096-0937
  • [Journal-full-title] Fungal genetics and biology : FG & B
  • [ISO-abbreviation] Fungal Genet. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA Primers; 0 / Fluorescent Dyes; 0 / Fungal Proteins; 0 / Xanthenes; 82354-19-6 / Texas red; EC 1.14.19.1 / Stearoyl-CoA Desaturase; EC 1.15.1.1 / Superoxide Dismutase; EC 5.2.1.8 / Peptidylprolyl Isomerase
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22. Liu L, Lu HZ, Henry M, Tamalet C: Polymorphism and drug selected mutations of reverse transcriptase gene in 102 HIV-1 infected patients living in China. J Med Virol; 2007 Oct;79(10):1593-9
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  • Few data are available for genotypic patterns within human immunodeficiency virus-1 (HIV-1) reverse transcriptase (RT) in drug-naive patients and RT inhibitor (RTI) treated patients in China.
  • This study aimed at characterizing the polymorphism of RT HIV-1 in the absence of drug treatment and to identify known and unknown mutations emerging under RTI selective pressure.
  • The HIV-1 RT gene from 21 drug-naive patients and 81 RTI treated patients from three provinces in China was analyzed.
  • In treated patients, two patterns of resistance associated mutations (RAMs) were observed:.
  • (1) K65R (9.8%), L74V (7.4%), M184V (7.4%), Q151M (5%), and thymidine analogue mutations (TAMs) (9.3%) including T215Y (5.5%), in patients who underwent ddI + d4T + NVP. (2) T215Y (23%), M184V (20%), and TAMs (15.4%) in patients receiving d4T + 3TC + EFV.

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  • [Copyright] (c) 2007 Wiley-Liss, Inc.
  • (PMID = 17705165.001).
  • [ISSN] 0146-6615
  • [Journal-full-title] Journal of medical virology
  • [ISO-abbreviation] J. Med. Virol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Reverse Transcriptase Inhibitors; EC 2.7.7.49 / HIV Reverse Transcriptase
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23. Garrè ML, Tekautz T: Role of high-dose chemotherapy (HDCT) in treatment of atypical teratoid/rhabdoid tumors (AT/RTs). Pediatr Blood Cancer; 2010 Apr;54(4):647-8
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  • [Title] Role of high-dose chemotherapy (HDCT) in treatment of atypical teratoid/rhabdoid tumors (AT/RTs).
  • Atypical teratoid/rhabdoid tumors (AT/RTs) of the CNS have been recently characterized as a distinct clinicopathologic entity with an unusually poor prognosis and with the highest incidence in the first 2 years of life.
  • AT/RT of the CNS is a usually fatal disease virtually unresponsive to chemotherapy (CT) and radiotherapy (RT).
  • Rapid progression and CNS dissemination are commonly reported.
  • Whether combined regimens including high-dose CT are able to prolong survival or change the natural history of this tumor are under evaluation.
  • [MeSH-major] Antineoplastic Agents / therapeutic use. Central Nervous System Neoplasms / drug therapy. Rhabdoid Tumor / drug therapy. Teratoma / drug therapy

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  • (PMID = 20146222.001).
  • [ISSN] 1545-5017
  • [Journal-full-title] Pediatric blood & cancer
  • [ISO-abbreviation] Pediatr Blood Cancer
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antineoplastic Agents
  • [Number-of-references] 18
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24. Boulmay BC, Chera BS, Morris CG, Kirwan J, Riggs CE, Lawson M, Mendenhall WM: Definitive altered fractionation radiotherapy and concomitant weekly cisplatin for locally advanced head and neck cancer. Am J Clin Oncol; 2009 Oct;32(5):488-91
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  • BACKGROUND: The purpose of this study was to determine the efficacy and toxicities of single-agent weekly cisplatin for patients with squamous cell carcinoma of the head and neck treated definitively with radiation therapy (RT).
  • METHODS: Thirty-five patients with American Joint Committee of Cancer stage II (3%), stage III (14%), or stage IV (83%) squamous cell carcinoma of the oropharynx, larynx, or hypopharynx treated from June 2000 to November 2003 at the University of Florida were retrospectively reviewed.
  • Subjects received radiation therapy (RT; median, 74.4 Gy) and cisplatin, 30 mg/m2/wk.
  • The endpoints were best response, percentage of grade III or IV toxicities, local-regional control, disease-free survival, cause-specific survival, and overall survival.
  • Grade III or IV toxicities were: anemia, 11%; thrombocytopenia, 6%; leukopenia, 26%; and mucositis, 23%.
  • CONCLUSION: Concomitant weekly CDDP with definitive RT is feasible, tolerable, highly active, and comparable with more complex, costly, and toxic regimens.

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  • (PMID = 19528791.001).
  • [ISSN] 1537-453X
  • [Journal-full-title] American journal of clinical oncology
  • [ISO-abbreviation] Am. J. Clin. Oncol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antineoplastic Agents; Q20Q21Q62J / Cisplatin
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25. Létourneau D, Wong R, Moseley D, Sharpe MB, Ansell S, Gospodarowicz M, Jaffray DA: Online planning and delivery technique for radiotherapy of spinal metastases using cone-beam CT: image quality and system performance. Int J Radiat Oncol Biol Phys; 2007 Mar 15;67(4):1229-37
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  • [Title] Online planning and delivery technique for radiotherapy of spinal metastases using cone-beam CT: image quality and system performance.
  • PURPOSE: To assess the feasibility of an online strategy for palliative radiotherapy (RT) of spinal bone metastasis, which integrates imaging, planning, and treatment delivery in a single step at the treatment unit.
  • METHODS AND MATERIALS: An integrated imaging, planning, and delivery system was constructed and tested with phantoms.
  • The magnitude of CBCT image artifacts following the use of an antiscatter grid and a nonlinear scatter correction was quantified using phantom data and images of patients receiving conventional palliative RT of the spine.
  • RESULTS: The use of image corrections reduced the cupping artifact from 30% to 4.5% on CBCT images of a body phantom and improved the accuracy of CBCT numbers (water: +/- 20 Hounsfield unit [HU], and lung and bone: to within +/- 130 HU).
  • The mean total time (n = 5) for application of the online approach was 23.1 min.
  • The process was performed in one session equivalent to an initial treatment slot (<30 min) with dosimetric accuracy satisfying accepted RT standards.

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  • (PMID = 17336223.001).
  • [ISSN] 0360-3016
  • [Journal-full-title] International journal of radiation oncology, biology, physics
  • [ISO-abbreviation] Int. J. Radiat. Oncol. Biol. Phys.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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26. Yari F, Zavaran Hosseini A, Nemat Gorgani M, Khorramizadeh MR, Mansouri P, Kazemnejad A: Expression of HLA-G in the skin of patients with pemphigus vulgaris. Iran J Allergy Asthma Immunol; 2008 Mar;7(1):7-12
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  • RT-PCR was performed to assess the patterns of HLA-G mRNA transcripts in the epidermal cells of PV and normal subjects.
  • In addition, the RT-PCR patterns of HLA-G amplification revealed a reduction in HLA-G2 and an increase in HLA-G1 transcripts in epidermal cells of PV patients as compared to normal cells.

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  • (PMID = 18322306.001).
  • [ISSN] 1735-1502
  • [Journal-full-title] Iranian journal of allergy, asthma, and immunology
  • [ISO-abbreviation] Iran J Allergy Asthma Immunol
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Iran
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / HLA Antigens; 0 / HLA-G Antigens; 0 / Histocompatibility Antigens Class I
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27. Kolli S, Lako M, Figueiredo F, Mudhar H, Ahmad S: Loss of corneal epithelial stem cell properties in outgrowths from human limbal explants cultured on intact amniotic membrane. Regen Med; 2008 May;3(3):329-42
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  • In addition, the expression of DeltaNp63, ABCG2 (both putative positive LSC markers) and cytokeratin K3 (marker of corneal differentiation) were assessed using quantitative reverse transcription PCR (RT-PCR).
  • Immunohistochemistry on paraffin-embedded sections was also performed to demonstrate protein localization and allow further confirmation of the quantitative RT-PCR results.
  • Quantitative RT-PCR revealed that the expression of the positive putative LSC markers DeltaNp63 and ABCG2 also showed a steady decrease in the zones furthest from the explant (p < 0.05 and p < 0.005, respectively).
  • Immunohistochemistry on paraffin-embedded sections of intact ex vivo-expanded limbal epithelium for the putative positive marker p63 and cytokeratin K3 confirmed the findings of the quantitative RT-PCR and CFE results.
  • CONCLUSIONS: We demonstrate for the first time that outgrowths from human limbal explants, a widely used technique in ex vivo expansion of LSCs for clinical transplantation, show a steady decline in a wide range of stem cell properties with distance from the central explant.

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  • (PMID = 18462056.001).
  • [ISSN] 1746-076X
  • [Journal-full-title] Regenerative medicine
  • [ISO-abbreviation] Regen Med
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 68238-35-7 / Keratins
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28. Troisi A, Orlandi G: Charge-transport regime of crystalline organic semiconductors: diffusion limited by thermal off-diagonal electronic disorder. Phys Rev Lett; 2006 Mar 3;96(8):086601
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  • We propose that the electron transport in crystalline organic semiconductors at room temperature (RT) is neither polaronic nor a combination of thermally activated hopping and polaronic transport, as previously thought.
  • This effect destroys the translational symmetry of the electronic Hamiltonian and makes the band description inadequate for RT organic crystals.

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  • (PMID = 16606209.001).
  • [ISSN] 0031-9007
  • [Journal-full-title] Physical review letters
  • [ISO-abbreviation] Phys. Rev. Lett.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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29. DenBesten PK, Machule D, Zhang Y, Yan Q, Li W: Characterization of human primary enamel organ epithelial cells in vitro. Arch Oral Biol; 2005 Aug;50(8):689-94
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  • [Title] Characterization of human primary enamel organ epithelial cells in vitro.
  • The expression of enamel matrix proteins was analyzed by RT-PCR and cytokeratin 14 was detected by immunohistochemistry.
  • Amelogenin, ameloblastin, enamelin, MMP-20 and KLK-4 were detected in CAB cells by RT-PCR.

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  • (PMID = 15958200.001).
  • [ISSN] 0003-9969
  • [Journal-full-title] Archives of oral biology
  • [ISO-abbreviation] Arch. Oral Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / AMBN protein, human; 0 / Amelogenin; 0 / Dental Enamel Proteins; 0 / Membrane Proteins; 0 / tuftelin; 68238-35-7 / Keratins; EC 3.4.21.- / Kallikreins; EC 3.4.21.- / kallikrein 4; EC 3.4.24.- / MMP20 protein, human; EC 3.4.24.- / Matrix Metalloproteinase 20; EC 3.4.24.- / Matrix Metalloproteinases; SY7Q814VUP / Calcium
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30. Phillips MD, Flynn MG, McFarlin BK, Stewart LK, Timmerman KL: Resistance training at eight-repetition maximum reduces the inflammatory milieu in elderly women. Med Sci Sports Exerc; 2010 Feb;42(2):314-25
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  • [Title] Resistance training at eight-repetition maximum reduces the inflammatory milieu in elderly women.
  • We examined the influence of moderate- to high-intensity resistance trainings (RT) on inflammatory cytokines (interleukin 6 (IL-6) and 1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha)) in circulation and lipopolysaccharide (LPS)-stimulated whole blood in elderly women.
  • Participants in the HRT, SER, and NHR groups trained (three sets, 10 exercises at eight-repetition maximum (8RM)) 3 d x wk(-1), whereas participants in the CON group maintained their "normal" activity for 10 wk.
  • Participants performed a bout of resistance exercise (RE at 8RM; HRT, SER, and NHR groups) or sat quietly (CON) before (BT) and after (AT) RT to assess the influence of training on the acute responses to RE.
  • Blood samples were obtained preexercise (PR), postexercise (PO), and 2 h postexercise (2H; same time points for resting CON).
  • RT significantly reduced resting serum TNF-alpha level by 37%.
  • RT also reduced LPS-stimulated production of IL-6, IL-1beta, and TNF-alpha at all time points (PR, PO, and 2H; per monocyte).
  • CONCLUSIONS: We found that 10 wk of moderate- to high-intensity RT 1) reduced the systemic inflammatory milieu and 2) abrogated exercise-induced circulating IL-6 in previously sedentary elderly women.
  • [MeSH-major] Interleukin-1beta / metabolism. Interleukin-6 / metabolism. Lipopolysaccharides / metabolism. Resistance Training. Tumor Necrosis Factor-alpha / metabolism

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  • (PMID = 19927028.001).
  • [ISSN] 1530-0315
  • [Journal-full-title] Medicine and science in sports and exercise
  • [ISO-abbreviation] Med Sci Sports Exerc
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Interleukin-1beta; 0 / Interleukin-6; 0 / Lipopolysaccharides; 0 / Tumor Necrosis Factor-alpha
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31. Thompson A, van der Slik AR, Koning F, van Bergen J: An improved RT-PCR method for the detection of killer-cell immunoglobulin-like receptor (KIR) transcripts. Immunogenetics; 2006 Nov;58(11):865-72
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  • [Title] An improved RT-PCR method for the detection of killer-cell immunoglobulin-like receptor (KIR) transcripts.
  • In this study, a reverse transcriptase-polymerase chain reaction (RT-PCR) that uses new primer sets for the gene-specific detection of KIR transcripts is presented and validated.
  • The key advantage of this RT-PCR method over previously published ones is that it was designed to detect transcripts of all confirmed allelic variants of the KIR genes, while remaining gene-specific.

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  • (PMID = 17033821.001).
  • [ISSN] 0093-7711
  • [Journal-full-title] Immunogenetics
  • [ISO-abbreviation] Immunogenetics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Validation Studies
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA Primers; 0 / RNA, Messenger; 0 / Receptors, Immunologic; 0 / Receptors, KIR
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32. Mendenhall WM, Amdur RJ, Morris CG, Kirwan J, Malyapa RS, Vaysberg M, Werning JW, Mendenhall NP: Carcinoma of the nasal cavity and paranasal sinuses. Laryngoscope; 2009 May;119(5):899-906
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  • OBJECTIVES/HYPOTHESIS: To determine the outcomes after radiotherapy (RT) alone or combined with surgery at the University of Florida for patients with carcinomas of the nasal cavity and paranasal sinuses.
  • Fifty-six patients were treated with definitive RT, and 53 patients received surgery and preoperative (eight patients) or postoperative (45 patients) RT.
  • Local control at 5 years was 43% after definitive RT versus 84% after surgery and adjuvant RT (P < .0001).
  • Multivariate analysis of local control revealed that both overall stage and treatment group (definitive RT versus surgery and adjuvant RT) significantly impacted this endpoint.
  • Cause-specific survival rates were: stages I to III, 81%; stage IV, 54%; and overall, 62%.
  • Thirty-one (20%) of 109 patients sustained severe complications; 17 of 56 patients (16%) after definitive RT and 14 of 53 patients (25%) after surgery and adjuvant RT.
  • CONCLUSIONS: The probability of local control and cause-specific survival is better after surgery and RT compared with definitive RT.
  • There is a modest increase in the risk of complications after surgery and RT.
  • Thus, the preferred treatment is surgery combined with preoperative or postoperative RT.
  • [MeSH-minor] Combined Modality Therapy. Female. Humans. Male. Nasal Cavity / pathology. Neoplasm Staging. Radiotherapy Dosage. Radiotherapy, Adjuvant. Survival Rate. Treatment Outcome

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  • (PMID = 19358246.001).
  • [ISSN] 1531-4995
  • [Journal-full-title] The Laryngoscope
  • [ISO-abbreviation] Laryngoscope
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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33. Levi JE, Wendel S, Takaoka DT, Silva IC, Castro JP, Torezan-Filho MA, Ghaname J, Gioachini R, Brandão J, Landi EP, Teixeira AC, Durigon EL: Replacement of HIV p24 Ag test by a multiplex RT-PCR method for primary screening of blood donors. Rev Inst Med Trop Sao Paulo; 2007 May-Jun;49(3):171-6
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  • [Title] Replacement of HIV p24 Ag test by a multiplex RT-PCR method for primary screening of blood donors.
  • Nucleic Acid Testing (NAT) as a tool for primary screening of blood donors became a reality in the end of the 1990 decade.
  • We report here the development of an "in-house" RT-PCR method that allows the simultaneous (multiplex) detection of HCV and HIV-RNA in addition to an artificial RNA employed as an external control.

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  • (PMID = 17625695.001).
  • [ISSN] 0036-4665
  • [Journal-full-title] Revista do Instituto de Medicina Tropical de São Paulo
  • [ISO-abbreviation] Rev. Inst. Med. Trop. Sao Paulo
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] Brazil
  • [Chemical-registry-number] 0 / HIV Core Protein p24; 0 / RNA, Viral
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34. Moldenhauer A, Wolf J, Habermann G, Genter G, Kiesewetter H, Salama A: Optimum storage conditions for cord blood-derived hematopoietic progenitor cells prior to isolation. Bone Marrow Transplant; 2007 Nov;40(9):837-42
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  • We therefore evaluated CD34+ cells isolated from cord blood units (n=57) within 1 h after collection and following storage for 24, 48 and 72 h at either room temperature (RT) or 4 degrees C.
  • After 48 h, the number of CD34+ cells increased compared to 24 h, if the cord blood had been stored at RT resulting in almost three times more CD34+ cells than at 4 degrees C.
  • These cells had a lower early apoptosis rate and formed four times more BFU-E than those stored at 4 degrees C with equivalent plating efficiencies.
  • CD34+ cells kept at RT for 48 h had the highest transmigration capacities, which paralleled an increased CXCR-4 expression.
  • Cord blood should be stored at RT before CD34+ isolation and a storage time for 48 h should be preferred to 24 h.

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  • (PMID = 17768391.001).
  • [ISSN] 0268-3369
  • [Journal-full-title] Bone marrow transplantation
  • [ISO-abbreviation] Bone Marrow Transplant.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antigens, CD34
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35. Ritchie SA, van den Hurk AF, Zborowski P, Kerlin TJ, Banks D, Walker JA, Lee JM, Montgomery BL, Smith GA, Pyke AT, Smith IL: Operational trials of remote mosquito trap systems for Japanese encephalitis virus surveillance in the Torres Strait, Australia. Vector Borne Zoonotic Dis; 2007;7(4):497-506
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  • However, pigs are amplifying hosts for JEV, and thus pose a health risk to the public and to pig handlers who bleed the pigs.
  • A remote mosquito trap system would not have these risks.
  • We report on trials using a remote mosquito trap system for the surveillance of JEV in the Torres Strait.
  • The Mosquito Magnet (MM) Pro, MM Liberty Plus, and a novel updraft trap, the NAQS Mozzie Trap, were run at Badu and Moa islands in the Torres Strait and at Bamaga in the northern Cape York Peninsula from 2002-2005.
  • TaqMan real-time polymerase chain reaction (PCR) was used to detect JEV nucleic acid in weekly mosquito collections.
  • Sentinel pigs located at Badu were also bled and the serum processed by reverse transcriptase (RT)-PCR for JEV antigen and enzyme-linked immunosorbent assay (ELISA) for anti-JEV antibodies.
  • The mosquito trap system cost ca.
  • AU$10,000 per site, about AU$5,000 less than a pig-based system.
  • Nonetheless, a remote mosquito trap system, employing stand alone traps and PCR for viral antigen detection, can be a safe, economical way to detect arbovirus activity in remote areas.

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  • (PMID = 18021024.001).
  • [ISSN] 1530-3667
  • [Journal-full-title] Vector borne and zoonotic diseases (Larchmont, N.Y.)
  • [ISO-abbreviation] Vector Borne Zoonotic Dis.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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36. Choo R, Pearse M, Danjoux C, Gardner S, Morton G, Szumacher E, Loblaw DA, Cheung P: Analysis of gastrointestinal and genitourinary morbidity of postoperative radiotherapy for pathologic T3 disease or positive surgical margins after radical prostatectomy using national cancer institute expanded common toxicity criteria. Int J Radiat Oncol Biol Phys; 2008 Nov 15;72(4):989-95
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • PURPOSE: To evaluate the acute and late gastrointestinal (GI) and genitourinary (GU) toxicity of postoperative radiotherapy (RT) after radical prostatectomy (RP).
  • METHODS AND MATERIALS: A total of 78 patients with pT3 or positive surgical margins after RP were treated with RT plus 2 years of androgen suppression, according to a Phase II study.
  • The median interval between RP and postoperative RT was 4.2 months.
  • Of these patients, 66%, 29%, and 1% experienced Grade 1, 2, and 3 acute GI or GU toxicity, respectively.
  • The cumulative incidence of Grade 2 or greater and Grade 3 or greater late GI toxicity at 36 months was 8.1% and 0%, respectively.
  • The cumulative incidence of Grade 2 or greater and Grade 3 or greater late GU toxicity at 36 months was 16.4% and 2.7%, respectively.
  • None had Grade 4 or greater late toxicity.
  • The severity of acute GU toxicity (less than Grade 2 vs. Grade 2 or greater) was a significant predictor factor for Grade 2 or greater late GU toxicity after adjusting for pre-existing GU dysfunction.
  • CONCLUSIONS: Postoperative RT was generally well tolerated.
  • Grade 3 or greater late GI or GU toxicity was uncommon.
  • [MeSH-major] Gastrointestinal Diseases / etiology. Male Urogenital Diseases / etiology. Neoplasm Recurrence, Local / prevention & control. Prostatectomy. Prostatic Neoplasms / radiotherapy. Prostatic Neoplasms / surgery. Radiation Injuries / etiology. Radiotherapy, Adjuvant / adverse effects

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  • (PMID = 18436391.001).
  • [ISSN] 1879-355X
  • [Journal-full-title] International journal of radiation oncology, biology, physics
  • [ISO-abbreviation] Int. J. Radiat. Oncol. Biol. Phys.
  • [Language] eng
  • [Publication-type] Clinical Trial; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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37. Hirano S, Tateya I, Kitamura M, Kada S, Ishikawa S, Kanda T, Tanaka S, Ito J: Ten years single institutional experience of treatment for advanced hypopharyngeal cancer in Kyoto University. Acta Otolaryngol Suppl; 2010 Nov;(563):56-61
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  • Induction chemotherapy has the potential to extend organ preservation therapy even in cases with locally advanced primary lesion.
  • It is also important to develop a strategy to reduce distant metastasis and to keep track of second primary cancers.
  • METHODS: A total of 72 cases with stage III/IV hypopharyngeal cancer were treated at Kyoto University Hospital during 2000-2008.
  • Surgery was performed in 56 cases; total pharyngolaryngoesophagectomy (TPLE) in 39 cases and partial pharyngectomy (PPX) preserving the larynx in 17 cases.
  • Radiotherapy (RT) with or without concurrent chemotherapy was applied in 16 cases.
  • DSS rates in cases treated with surgery and those with RT were 65.1% and 56.1%, respectively.
  • Local control rates for cases treated with TPLE, PPX, and RT were 97.3%, 100%, and 80.4%, respectively.
  • In the end, 17 cases died of the primary disease, while 10 cases died of other causes, mainly second primary cancers.
  • [MeSH-minor] Adult. Aged. Aged, 80 and over. Cohort Studies. Combined Modality Therapy. Female. Hospitals, University. Humans. Japan. Male. Middle Aged. Neoplasm Staging. Pharyngectomy. Retrospective Studies. Survival Rate. Time Factors. Treatment Outcome

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  • (PMID = 20879820.001).
  • [ISSN] 0365-5237
  • [Journal-full-title] Acta oto-laryngologica. Supplementum
  • [ISO-abbreviation] Acta Otolaryngol Suppl
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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38. Wo JY, Taghian AG, Nguyen PL, Raad RA, Sreedhara M, Bellon JR, Wong JS, Gadd MA, Smith BL, Harris JR: The association between biological subtype and isolated regional nodal failure after breast-conserving therapy. Int J Radiat Oncol Biol Phys; 2010 May 1;77(1):188-96
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  • PURPOSE: To evaluate the risk of isolated regional nodal failure (RNF) among women with invasive breast cancer treated with breast-conserving surgery (BCS) and radiation therapy (RT) and to determine factors, including biological subtype, associated with RNF.
  • METHODS AND MATERIALS: We retrospectively studied 1,000 consecutive women with invasive breast cancer who received breast-conserving surgery and RT from 1997 through 2002.
  • Biological subtype classification into luminal A, luminal B, HER-2, and basal subtypes was based on estrogen receptor status-, progesterone receptor status-, and HER-2-status of the primary tumor.
  • On univariate analysis, biological subtype (p = 0.0002), lymph node involvement (p = 0.008), lymphovascular invasion (p = 0.02), and Grade 3 histology (p = 0.01) were associated with significantly higher RNF rates.
  • Low rates of RNF in patients with one to three positive nodes suggest that tangential RT without RNI is reasonable in most patients.
  • [MeSH-minor] Adult. Aged. Aged, 80 and over. Analysis of Variance. Chemotherapy, Adjuvant. Female. Follow-Up Studies. Humans. Lymph Node Excision. Lymph Nodes / pathology. Lymphatic Irradiation. Mastectomy, Segmental. Neoplasm Invasiveness. Prognosis. Receptor, ErbB-2 / analysis. Receptors, Estrogen / analysis. Receptors, Progesterone / analysis. Retrospective Studies. Risk. Sentinel Lymph Node Biopsy / utilization

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  • (PMID = 20171798.001).
  • [ISSN] 1879-355X
  • [Journal-full-title] International journal of radiation oncology, biology, physics
  • [ISO-abbreviation] Int. J. Radiat. Oncol. Biol. Phys.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Receptors, Estrogen; 0 / Receptors, Progesterone; EC 2.7.10.1 / Receptor, ErbB-2
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39. MacDonald TJ, Arenson EB, Ater J, Sposto R, Bevan HE, Bruner J, Deutsch M, Kurczynski E, Luerssen T, McGuire-Cullen P, O'Brien R, Shah N, Steinbok P, Strain J, Thomson J, Holmes E, Vezina G, Yates A, Phillips P, Packer R: Phase II study of high-dose chemotherapy before radiation in children with newly diagnosed high-grade astrocytoma: final analysis of Children's Cancer Group Study 9933. Cancer; 2005 Dec 15;104(12):2862-71
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  • [Title] Phase II study of high-dose chemotherapy before radiation in children with newly diagnosed high-grade astrocytoma: final analysis of Children's Cancer Group Study 9933.
  • BACKGROUND: High-grade astrocytomas (HGA) carry a dismal prognosis and compose nearly 20% of all childhood brain tumors.
  • METHODS: In a nationwide study, The Children's Cancer Group (CCG) prospectively evaluated 102 children with HGA and postoperative residual disease for efficacy and toxicity of four courses of HDCT before radiotherapy (RT).
  • After HDCT, all patients were to receive local RT followed by lomustine and vincristine.
  • Twenty-six patients were excluded after central neuroradiographic review (n = 8) or pathology review (n = 18).
  • Nonhematologic serious toxicities were common (29%), and 21% of patients did not receive RT.
  • Median time to an event was longest for Regimen A (283 days compared with 83 and 91 days for Regimens B and C, respectively).
  • CONCLUSIONS: OS and EFS were not affected by histologic grade.
  • Patients who responded to HDCT had a nominally higher survival rate (P = 0.03 for trend).
  • The authors conclude that these commonly used HDCT regimens provide no additional clinical benefit to conventional treatment in HGA, regardless of the amount of measurable residual tumor.
  • [MeSH-minor] Adolescent. Adult. Brain Stem Neoplasms / drug therapy. Brain Stem Neoplasms / mortality. Brain Stem Neoplasms / pathology. Brain Stem Neoplasms / radiotherapy. Child. Child, Preschool. Combined Modality Therapy. Disease-Free Survival. Dose-Response Relationship, Drug. Drug Administration Schedule. Female. Humans. Male. Neoplasm Staging. Probability. Prognosis. Prospective Studies. Radiotherapy, High-Energy. Reference Values. Risk Assessment. Spinal Cord Neoplasms / drug therapy. Spinal Cord Neoplasms / mortality. Spinal Cord Neoplasms / pathology. Spinal Cord Neoplasms / radiotherapy. Survival Analysis. Time Factors. Treatment Outcome

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  • [Copyright] Copyright 2005 American Cancer Society.
  • (PMID = 16315242.001).
  • [ISSN] 0008-543X
  • [Journal-full-title] Cancer
  • [ISO-abbreviation] Cancer
  • [Language] eng
  • [Publication-type] Clinical Trial, Phase II; Journal Article; Randomized Controlled Trial
  • [Publication-country] United States
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40. Katz MS, Efstathiou JA, D'Amico AV, Kattan MW, Sanda MG, Nguyen PL, Smith MR, Carroll PR, Zietman AL: The 'CaP Calculator': an online decision support tool for clinically localized prostate cancer. BJU Int; 2010 May;105(10):1417-22
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  • Estimates of the prostate-specific antigen (PSA) outcome after radical prostatectomy (RP) or radiotherapy (RT), and clinical outcomes after RT, were also entered.
  • The 5-year estimates of PSA relapse-free survival after RT and 10-year estimates after RP were available.

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  • [Cites] Arch Pathol Lab Med. 2003 Aug;127(8):1007-8 [12873176.001]
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  • (PMID = 20346051.001).
  • [ISSN] 1464-410X
  • [Journal-full-title] BJU international
  • [ISO-abbreviation] BJU Int.
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / K24 CA121990; United States / NCI NIH HHS / CA / K24 CA121990-05
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] EC 3.4.21.77 / Prostate-Specific Antigen
  • [Number-of-references] 30
  • [Other-IDs] NLM/ NIHMS272528; NLM/ PMC3090683
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41. Salzmann GM, Paul J, Bauer JS, Woertler K, Sauerschnig M, Landwehr S, Imhoff AB, Schöttle PB: T2 assessment and clinical outcome following autologous matrix-assisted chondrocyte and osteochondral autograft transplantation. Osteoarthritis Cartilage; 2009 Dec;17(12):1576-82
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  • While previous clinical studies have compared the respective outcome, there is no such information investigating the ultrastructural composition using T2 mapping comparing cartilage T2 values of the repair tissue (RT).
  • RESULTS: Global T2 values of healthy femoral cartilage (HC) were similar among groups, while T2 values of the RT following MACT (46.8ms, SD 8.6) were significantly lower when compared to RT T2 values after OCT (55.5ms, SD 6.7) (P=0.048).

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  • (PMID = 19737529.001).
  • [ISSN] 1522-9653
  • [Journal-full-title] Osteoarthritis and cartilage
  • [ISO-abbreviation] Osteoarthr. Cartil.
  • [Language] ENG
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] England
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42. Bai J, Lü Y, Bai Q: Construction of porcine CCK pDNA and its expression in COS-7 cells. J Huazhong Univ Sci Technolog Med Sci; 2007 Jun;27(3):278-80
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  • Total RNA was extracted from porcine intestinal mucosa.
  • RT-PCR was used to amplify the aimed segments CCKcDNA which was then digested with EcoR1 and BamH1 and inserted into a eukaryotic expression plasmid pIRES2-EGFP to construct CCK pDNA.
  • The expression of CCK in transfected COS-7 cells was detected 24, 48 and 72 h post-transfection with fluorescence microscopy and the expression level of CCK mRNA in transfected COS-7 cells was assayed by using RT-PCR.
  • The expression of CCK mRNA in the cells was detectable by using RT-PCR.
  • It was concluded CCK pDNA was expressed successfully in COS-7 cells, which lays a foundation for further research on the relationship between CCK and tumor.

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  • (PMID = 17641841.001).
  • [ISSN] 1672-0733
  • [Journal-full-title] Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban
  • [ISO-abbreviation] J. Huazhong Univ. Sci. Technol. Med. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / DNA, Complementary; 0 / RNA, Messenger; 0 / Recombinant Fusion Proteins; 147336-22-9 / Green Fluorescent Proteins; 9011-97-6 / Cholecystokinin
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43. Hulmi JJ, Tannerstedt J, Selänne H, Kainulainen H, Kovanen V, Mero AA: Resistance exercise with whey protein ingestion affects mTOR signaling pathway and myostatin in men. J Appl Physiol (1985); 2009 May;106(5):1720-9
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  • [Title] Resistance exercise with whey protein ingestion affects mTOR signaling pathway and myostatin in men.
  • The effects of whey protein ingestion on acute and long-term signaling responses of resistance exercise are not well known.
  • Vastus lateralis (VL) muscle biopsies were taken before and 1 h and 48 h after a leg press of 5 x 10 repetitions [resistance exercise (RE)] and after 21 wk (2 times per week) of resistance training (RT).
  • Protein (15 g of whey) or nonenergetic placebo was ingested before and after a single RE bout and each RE workout throughout the RT.
  • Mammalian target of rapamycin (mTOR) phosphorylation was increased after the RE bout and RT only in the protein group, whereas the protein ingestion prevented the post-RE decrease in phosphorylated eukaryotic initiation factor 4E binding protein 1 (p-4E-BP1).
  • Akt phosphorylation decreased after RT, whereas no change was observed in phosphorylated eukaryotic elongation factor 2.
  • The results indicate that resistance exercise rapidly increases mTOR signaling and may decrease myostatin protein expression in muscle and that whey protein increases and prolongs the mTOR signaling response.


44. Rödel F, Keilholz L, Herrmann M, Weiss C, Frey B, Voll R, Gaipl U, Rödel C: Activator protein 1 shows a biphasic induction and transcriptional activity after low dose X-irradiation in EA.hy.926 endothelial cells. Autoimmunity; 2009 May;42(4):343-5
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  • Low dose radiotherapy (LD-RT) is known to exert an anti-inflammatory effect.
  • We have recently shown that LD-RT of stimulated EA.hy.926 endothelial cells (EC) resulted in a biphasic DNA-binding and transcriptional activity of NF-kappaB in parallel with a biphasic course of leukocyte adhesion.
  • In conclusion, the discontinuous dose response of AP-1 activation may add a further facet to the plethora of mechanisms contributing to the anti-inflammatory efficacy of LD-RT.

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  • (PMID = 19811296.001).
  • [ISSN] 1607-842X
  • [Journal-full-title] Autoimmunity
  • [ISO-abbreviation] Autoimmunity
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Transcription Factor AP-1
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45. Hoskin PJ, Rojas AM, Bentzen SM, Saunders MI: Radiotherapy with concurrent carbogen and nicotinamide in bladder carcinoma. J Clin Oncol; 2010 Nov 20;28(33):4912-8
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  • PURPOSE: Phase II clinical studies suggest that hypoxic modification with carbogen and nicotinamide (CON) may increase the efficacy of radiotherapy (RT).
  • PATIENTS AND METHODS: Three hundred thirty-three patients with locally advanced bladder carcinoma were randomly assigned to RT alone versus RT with CON.
  • The primary end point was cystoscopic control at 6 months (CC(6m)) and secondary end points were overall survival (OS), local relapse-free survival (RFS), urinary and rectal morbidity.
  • RESULTS: CC(6m) was 81% for RT + CON and 76% for RT alone (P = .3); however, just more than half of patients underwent cystoscopy at that time.
  • Three-year estimates of OS were 59% and 46% (P = .04) and 3-year estimates of RFS were 54% and 43% (P = .06) for RT + CON versus RT alone.
  • Risk of death was 14% lower with RT + CON (P = .04).
  • In multivariate comparison, RT + CON significantly reduced the risk of relapse (P = .05) and death (P = .03).
  • CONCLUSION: RT + CON produced a small nonsignificant improvement in CC(6m).
  • Differences in OS, risk of death, and local relapse were significantly in favor of RT + CON.
  • Results indicate a benefit of adding CON to radical RT.
  • [MeSH-minor] Aged. Aged, 80 and over. Cystectomy. Female. Humans. Male. Middle Aged. Neoplasm Staging

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  • [CommentIn] Nat Rev Clin Oncol. 2011 Mar;8(3):129-30 [21283126.001]
  • (PMID = 20956620.001).
  • [ISSN] 1527-7755
  • [Journal-full-title] Journal of clinical oncology : official journal of the American Society of Clinical Oncology
  • [ISO-abbreviation] J. Clin. Oncol.
  • [Language] eng
  • [Grant] United Kingdom / Cancer Research UK / /
  • [Publication-type] Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Radiation-Sensitizing Agents; 142M471B3J / Carbon Dioxide; 25X51I8RD4 / Niacinamide; 8063-77-2 / carbogen; S88TT14065 / Oxygen
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46. Sanguineti G, Richetti A, Bignardi M, Corvo' R, Gabriele P, Sormani MP, Antognoni P: Accelerated versus conventional fractionated postoperative radiotherapy for advanced head and neck cancer: results of a multicenter Phase III study. Int J Radiat Oncol Biol Phys; 2005 Mar 1;61(3):762-71
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  • [Title] Accelerated versus conventional fractionated postoperative radiotherapy for advanced head and neck cancer: results of a multicenter Phase III study.
  • PURPOSE: To determine whether, in the postoperative setting, accelerated fractionation (AF) radiotherapy (RT) yields a superior locoregional control rate compared with conventional fractionation (CF) RT in locally advanced squamous cell carcinomas of the oral cavity, oropharynx, larynx, or hypopharynx.
  • METHODS AND MATERIALS: Patients from four institutions with one or more high-risk features (pT4, positive resection margins, pN >1, perineural/lymphovascular invasion, extracapsular extension, subglottic extension) after surgery were randomly assigned to either RT with one daily session of 2 Gy up to 60 Gy in 6 weeks or AF.
  • Accelerated fractionation consisted of a "biphasic concomitant boost" schedule, with the boost delivered during the first and last weeks of treatment, to deliver 64 Gy in 5 weeks.
  • The primary endpoint of the study was locoregional control.
  • However, interaction analysis with median values as cut-offs showed a trend for improved locoregional control for those patients who had a delay in starting RT and who were treated with AF compared with those with a similar delay but who were treated with CF (hazard ratio = 0.5, 95% confidence interval 0.2-1.1).
  • Although preliminary, actuarial Grade 3+ late toxicity estimates at 2 years were 18% +/- 4% and 27% +/- 6% for CF and AF, respectively (p = 0.10).
  • CONCLUSION: Accelerated fractionation does not seem to be worthwhile for squamous cell carcinoma of the head and neck after resection; however, AF might be an option for patients who delay starting RT.

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  • (PMID = 15708255.001).
  • [ISSN] 0360-3016
  • [Journal-full-title] International journal of radiation oncology, biology, physics
  • [ISO-abbreviation] Int. J. Radiat. Oncol. Biol. Phys.
  • [Language] eng
  • [Publication-type] Clinical Trial; Clinical Trial, Phase III; Journal Article; Multicenter Study; Randomized Controlled Trial
  • [Publication-country] United States
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47. Ross LL, Weinberg WG, DeJesus E, Fischl MA, Horton JH, Pappa KA, Lancaster CT, Pakes GE, Smith KY: Impact of low abundance HIV variants on response to ritonavir-boosted atazanavir or fosamprenavir given once daily with tenofovir/emtricitabine in antiretroviral-naive HIV-infected patients. AIDS Res Hum Retroviruses; 2010 Apr;26(4):407-17
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  • Population genotyping (PG) can underestimate resistance if resistance-containing low abundance variants go undetected.
  • VF was defined as confirmed HIV-1 RNA > or =400 copies/ml at > or =24 weeks or viral rebound >400 copies/ml any time following viral suppression.
  • Baseline resistance mutations were more prevalent in patients receiving FPV/r (10/53) than ATV/r (3/53).
  • In the four FPV/r-treated VFs, baseline HIV TAMs combinations and/or PI mutations were detected in one by PG at VF (RT: L210W + T215C; PR: M46I + L76V) and three others by CA alone (RT: L210W + T215Y; RT: M41L; RT: K65R + K70R; PR: I47V); all four had study drug-associated mutations (CA detecting more HIV-1 resistance mutations than PG).
  • In the three ATV/r VFs, no baseline drug-associated mutations were detected by PG; for one patient CA detected RT: K65R; PR: I84V.
  • Phylogenetic analysis revealed tight clustering for FPV/r-treated VFs with highly related clones, whereas HIV-1 from ATV/r-treated VFs had no outgrowth from baseline of low abundance resistance-containing variants.
  • In conclusion, low-abundance HIV resistance-containing variants were detected in baseline samples from patients with VF.
  • Despite this and a baseline resistance imbalance between the two arms, FPV/r and ATV/r provided similar virologic suppression through 48 weeks; however, these findings highlight the necessity for the development of quick and inexpensive methods for detection of minority species to better guide therapy selection.
  • [MeSH-minor] Adolescent. Adult. Aged. Atazanavir Sulfate. Drug Administration Schedule. Drug Resistance, Multiple, Viral / genetics. Drug Therapy, Combination. Emtricitabine. Humans. Middle Aged. RNA, Viral / analysis. RNA, Viral / genetics. Sequence Analysis, RNA / methods. Tenofovir. Treatment Failure

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  • (PMID = 20380480.001).
  • [ISSN] 1931-8405
  • [Journal-full-title] AIDS research and human retroviruses
  • [ISO-abbreviation] AIDS Res. Hum. Retroviruses
  • [Language] eng
  • [Publication-type] Clinical Trial; Journal Article; Multicenter Study; Randomized Controlled Trial; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antiviral Agents; 0 / Carbamates; 0 / Oligopeptides; 0 / Organophosphates; 0 / Organophosphonates; 0 / Pyridines; 0 / RNA, Viral; 0 / Sulfonamides; 0W860991D6 / Deoxycytidine; 4MT4VIE29P / Atazanavir Sulfate; 99YXE507IL / Tenofovir; G70B4ETF4S / Emtricitabine; JAC85A2161 / Adenine; O3J8G9O825 / Ritonavir; WOU1621EEG / fosamprenavir
  • [Other-IDs] NLM/ PMC2875981
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48. Aradaib IE: PCR detection of African horse sickness virus serogroup based on genome segment three sequence analysis. J Virol Methods; 2009 Jul;159(1):1-5
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  • A nested reverse transcriptase (RT) polymerase chain reaction (RT-PCR), for rapid detection of African horse sickness virus (AHSV) double-stranded ribonucleic acid (dsRNA) in cell culture and tissue samples, was developed and evaluated.
  • Using an outer pair of primers (P1 and P2), selected from genome segment three of AHSV serotype 6 (AHSV-6), the RT-PCR-based assay resulted in amplification of a 890 base pair (bp) primary PCR product.
  • RNAs from the nine vaccine strains of AHSV, and a number of AHSV field isolates including the Central African isolates of AHSV-9 and AHSV-6, propagated in cell cultures, were detected by this assay.
  • The RT-PCR described below detected as little as 0.1 fg of AHSV RNA, which is equivalent to six viral particles.
  • The nested amplification confirmed the integrity of the primary PCR product and increased the sensitivity of the PCR assay by at least 1000-fold.
  • Application of this RT-PCR assay to clinical samples resulted in direct detection of AHSV dsRNA from blood and a variety of tissue samples collected from equines infected experimentally and naturally.
  • The specificity studies indicated that the primary or the nested PCR products were not amplified from, closely related orbiviruses including, bluetongue virus (BTV) prototypes serotypes 1, 2, 4, 10, 16 and 17; epizootic hemorrhagic disease of deer virus (EHDV) prototypes serotypes 1 and 2; EHDV-318, Sudanese isolates of palyam serogroup of orbiviruses; total nucleic acid extracts from uninfected Vero cells; or unfractionated blood from horses and donkeys that were AHSV-seronegative and virus isolation negative.
  • The RT-PCR provides a valuable tool for study of the epidemiology of AHSV and can be recommended for rapid diagnosis during an outbreak of the disease among susceptible equines.

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  • (PMID = 19442836.001).
  • [ISSN] 1879-0984
  • [Journal-full-title] Journal of virological methods
  • [ISO-abbreviation] J. Virol. Methods
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA Primers; 0 / RNA, Viral
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49. Kawakami T, Terakawa Y, Tsuruno T, Murata T, Nishio A, Ohata K: Mechanical clot disruption following intravenous recombinant tissue plasminogen activator administration in non-responders. Neurol Med Chir (Tokyo); 2010;50(3):183-91
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  • The efficacy of selecting non-responders to intravenous recombinant tissue plasminogen activator (rt-PA) for mechanical clot disruption (MCD) was investigated based on cerebral angiography in the acute stage following rt-PA therapy. rt-PA therapy using 0.6 mg/kg was performed in eligible patients within 3 hours of onset.
  • Patients who did not show recanalization on cerebral angiography 1 hour after rt-PA initiation immediately underwent MCD.
  • Eighteen patients were initially treated with intravenous rt-PA, with mean time from stroke onset to rt-PA therapy of 120 +/- 27 minutes.
  • Time to recanalization by percutaneous transluminal angioplasty from stroke onset was 258 +/- 59 minutes.
  • MCD for non-responders determined by cerebral angiography at the end of intravenous rt-PA infusion can decrease the time to recanalization and improve recanalization rates, leading to good clinical outcome after acute stroke.
  • [MeSH-minor] Acute Disease. Aged. Aged, 80 and over. Brain Ischemia / therapy. Cerebral Angiography. Combined Modality Therapy. Female. Follow-Up Studies. Humans. Injections, Intravenous. Male. Middle Aged. Patient Selection. Recombinant Proteins. Time Factors. Treatment Failure. Treatment Outcome

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  • (PMID = 20339266.001).
  • [ISSN] 1349-8029
  • [Journal-full-title] Neurologia medico-chirurgica
  • [ISO-abbreviation] Neurol. Med. Chir. (Tokyo)
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Japan
  • [Chemical-registry-number] 0 / Fibrinolytic Agents; 0 / Recombinant Proteins; EC 3.4.21.68 / Tissue Plasminogen Activator
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50. Bergin PF, Doppelt JD, Hamilton WG, Mirick GE, Jones AE, Sritulanondha S, Helm JM, Tuan RS: Detection of periprosthetic infections with use of ribosomal RNA-based polymerase chain reaction. J Bone Joint Surg Am; 2010 Mar;92(3):654-63
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  • We have reported the development of a messenger RNA (mRNA)-based procedure to reduce these false-positive results.
  • The limitations of this procedure are the lack of a universal target and reduced sensitivity due to a low concentration of bacterial mRNAs in test samples.
  • The objective of the present study was to determine whether reverse transcription-quantitative polymerase chain reaction (RT-qPCR) using universal primers can be used to detect the more abundant bacterial ribosomal RNA (rRNA) as an indicator of periprosthetic infection.
  • METHODS: Serial dilutions of simulated synovial fluid infections were analyzed with rRNA RT-qPCR to determine the detection limit of this assay.
  • Escherichia coli cultures treated with gentamicin were analyzed with RT-qPCR over a twenty-day time course to determine the degradation of rRNA as compared with the decrease in the viable cell count as determined by means of cell plating.
  • Sixty-four patients with a symptomatic effusion at the site of a total knee arthroplasty were enrolled, and complete patient information was documented in a prospective manner.
  • Synovial fluid analysis with rRNA RT-qPCR was performed in a blind fashion.
  • RESULTS: The rRNA RT-qPCR assay was able to detect as few as 590 colony forming units/mL of Staphylococcus aureus and 2900 colony forming units/mL of Escherichia coli.
  • The rRNA RT-qPCR signal closely followed cell death, pointing to its potential use as a viability marker.
  • Clinically, the test correctly identified all six patients with a confirmed infection and all fifty patients who clearly did not have an infection.
  • Infection was indicated by rRNA RT-qPCR in three of these patients who had elevated synovial fluid white blood-cell counts but negative results on culture.
  • For statistical purposes, all patients who were categorized as indeterminate were considered to have an infection for the purpose of analysis, for a prevalence of 22% in this cohort.
  • CONCLUSIONS: With respect to current diagnostic tests, rRNA-based RT-qPCR demonstrated 100% specificity and positive predictive value with a sensitivity equivalent to that of intraoperative culture.
  • The RT-qPCR signal followed bacterial culture trends but exhibited detectable level for seven days after sterilization, allowing for the detection of infection after the antibiotic administration.
  • These findings indicate that rRNA RT-qPCR is a sensitive and reliable test that retains the universal detection and speciation of DNA-based methods while functioning as a viability indicator.

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  • (PMID = 20194324.001).
  • [ISSN] 1535-1386
  • [Journal-full-title] The Journal of bone and joint surgery. American volume
  • [ISO-abbreviation] J Bone Joint Surg Am
  • [Language] ENG
  • [Grant] United States / NIAMS NIH HHS / AR / Z01 AR041131; United States / Intramural NIH HHS / /
  • [Publication-type] Journal Article; Research Support, N.I.H., Intramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / RNA, Ribosomal; 9007-41-4 / C-Reactive Protein
  • [Other-IDs] NLM/ PMC2827826
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51. Aggarwal A, Singh M: Changes in skin and rectal temperature in lactating buffaloes provided with showers and wallowing during hot-dry season. Trop Anim Health Prod; 2008 Apr;40(3):223-8
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  • All the buffaloes were kept under loose housing system and were provided ad lib green maize fodder and water to drink during 30 days experiment during the month of August-September.
  • Showering group (SG) buffaloes were kept under water showers from 11:00 A.M. to 4:00 P.M., while wallowing group (WG) buffaloes were allowed to wallow in a water pond during the same time.
  • Physiological responses viz. rectal temperature (RT), respiration rate (RR), pulse rate (PR) and skin temperature (ST) were recorded before (8.00 A.M.) and after (4.00 P.M.) showers or wallowing.
  • Further, skin temperature of neck, head, udder, udder vein and RT varied (P < 0.01) in SG and WG buffaloes during periods of study.
  • The correlation data indicated a positive correlation of maximum air temperature with RT in SG but correlation was non-significant in WG.
  • RT was positively correlated with ST in SG (P < 0.05) and WG (P < 0.01).
  • The pooled data analysis of both groups also indicated a positive correlation of maximum temperature with RT (P < 0.05).

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  • (PMID = 18484125.001).
  • [ISSN] 0049-4747
  • [Journal-full-title] Tropical animal health and production
  • [ISO-abbreviation] Trop Anim Health Prod
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 059QF0KO0R / Water
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52. Goodwin A, Parker S, Ghersi D, Wilcken N: Post-operative radiotherapy for ductal carcinoma in situ of the breast. Cochrane Database Syst Rev; 2009;(1):CD000563
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  • BACKGROUND: The addition of radiotherapy (RT) following breast conserving surgery (BCS) was first shown to reduce the risk of ipsilateral recurrence in the treatment of invasive breast cancer.
  • Randomised controlled trials (RCTs) have shown that RT can reduce the risk of recurrence, but assessment of potential long-term complications from addition of RT following BSC for DCIS has not been reported for women participating in RCTs.
  • OBJECTIVES: To summarise the data from RCTs testing the addition of RT to BCS for treatment of DCIS to determine the balance between the benefits and harms.
  • SEARCH STRATEGY: We searched the Cochrane Breast Cancer Group Specialised Register (January 2008), Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2008, Issue 1), MEDLINE (February 2008), and EMBASE (February 2008).
  • Three trials compared the addition of RT to BCS.
  • One trial was a two by two factorial design comparing the use of RT and tamoxifen, each separately or together, in which participants were randomised in at least one arm.

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  • [UpdateIn] Cochrane Database Syst Rev. 2009;(3):CD000563 [19588320.001]
  • (PMID = 19160183.001).
  • [ISSN] 1469-493X
  • [Journal-full-title] The Cochrane database of systematic reviews
  • [ISO-abbreviation] Cochrane Database Syst Rev
  • [Language] eng
  • [Publication-type] Journal Article; Meta-Analysis; Review
  • [Publication-country] England
  • [Number-of-references] 24
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53. Torija MJ, Mateo E, Guillamón JM, Mas A: Identification and quantification of acetic acid bacteria in wine and vinegar by TaqMan-MGB probes. Food Microbiol; 2010 Apr;27(2):257-65
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  • A Real-Time PCR (RT-PCR) assay was developed using TaqMan minor groove binder (MGB) probes for the specific detection and quantification of five acetic acid bacteria (AAB) species (Acetobacter pasteurianus, Acetobacter aceti, Gluconacetobacter hansenii, Gluconacetobacter europaeus and Gluconobacter oxydans) in wine and vinegar.
  • The RT-PCR assay used in this study is a reliable, specific and fast method for quantifying these five AAB species in wine and vinegar.

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  • (PMID = 20141944.001).
  • [ISSN] 1095-9998
  • [Journal-full-title] Food microbiology
  • [ISO-abbreviation] Food Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Validation Studies
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA Probes; Q40Q9N063P / Acetic Acid
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54. Broeks A, Braaf LM, Huseinovic A, Schmidt MK, Russell NS, van Leeuwen FE, Hogervorst FB, Van 't Veer LJ: The spectrum of ATM missense variants and their contribution to contralateral breast cancer. Breast Cancer Res Treat; 2008 Jan;107(2):243-8
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  • We have determined the spectrum and frequency of ATM missense variants in 443 breast cancer patients diagnosed before age 50, including 247 patients who subsequently developed CBC.
  • We have found that carriers of an ATM missense mutation, who were treated with radiotherapy for the first breast tumour, developed their second tumour on average in a 92-month interval compared to a 136-month mean interval for those CBC patients who neither received RT nor carried a germline variant, (p = 0.029).
  • However, the combination of RT and (certain) ATM missense variants seems to accelerate tumour development.
  • [MeSH-major] Breast Neoplasms / genetics. Breast Neoplasms / metabolism. Cell Cycle Proteins / genetics. DNA-Binding Proteins / genetics. Mutation, Missense. Protein-Serine-Threonine Kinases / genetics. Tumor Suppressor Proteins / genetics

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  • (PMID = 17393301.001).
  • [ISSN] 1573-7217
  • [Journal-full-title] Breast cancer research and treatment
  • [ISO-abbreviation] Breast Cancer Res. Treat.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Cell Cycle Proteins; 0 / DNA-Binding Proteins; 0 / Tumor Suppressor Proteins; EC 2.7.11.1 / ATM protein, human; EC 2.7.11.1 / Ataxia Telangiectasia Mutated Proteins; EC 2.7.11.1 / Protein-Serine-Threonine Kinases
  • [Other-IDs] NLM/ PMC2137941
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55. Cai Y, Shen XZ, Zhou CH, Wang JY: Abnormal expression of Smurf2 during the process of rat liver fibrosis. Chin J Dig Dis; 2006;7(4):237-45
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  • Thereafter, a semi-quantitative RT-PCR, Western blot analysis and immunohistochemistry test were performed for determining Smurf2, Smad2 and SnoN at week 1, 2, 4 and 8 of establishing the liver fibrosis model.
  • RESULTS: Smurf2, FGG, PTAFR, CYP2D6, among others, increased in the fibrosis liver and a semi-quantitative RT-PCR confirmed the reliability of the cDNA microarray analysis.

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  • (PMID = 17054587.001).
  • [ISSN] 1443-9611
  • [Journal-full-title] Chinese journal of digestive diseases
  • [ISO-abbreviation] Chin J Dig Dis
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Australia
  • [Chemical-registry-number] 0 / Nerve Tissue Proteins; 0 / Smad2 Protein; 0 / SnoN protein, rat; 0 / Transcription Factors; EC 6.3.2.19 / Ubiquitin-Protein Ligases
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56. Coe H, Bedard K, Groenendyk J, Jung J, Michalak M: Endoplasmic reticulum stress in the absence of calnexin. Cell Stress Chaperones; 2008 Dec;13(4):497-507
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  • In this study, we used beta-galactosidase reporter gene knock-in and reverse transcriptase polymerase chain reaction (RT-PCR) to investigate activation of the calnexin gene during embryonic development.
  • In contrast, non-stimulated calnexin-deficient cells showed increased activation of IRE1, as measured by RT-PCR and luciferase reporter gene analysis of splicing of Xbp1 mRNA and activation of the BiP promoter.

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  • (PMID = 18528784.001).
  • [ISSN] 1355-8145
  • [Journal-full-title] Cell stress & chaperones
  • [ISO-abbreviation] Cell Stress Chaperones
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA-Binding Proteins; 0 / Heat-Shock Proteins; 0 / Molecular Chaperones; 0 / RNA, Messenger; 0 / Transcription Factors; 0 / molecular chaperone GRP78; 0 / regulatory factor X transcription factors; 139873-08-8 / Calnexin; EC 3.4.25.1 / Proteasome Endopeptidase Complex
  • [Other-IDs] NLM/ PMC2673926
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57. Longyant S, Sithigorngul P, Chaivisuthangkura P, Rukpratanporn S, Sithigorngul W, Menasveta P: Differences in susceptibility of palaemonid shrimp species to yellow head virus (YHV) infection. Dis Aquat Organ; 2005 Apr 6;64(1):5-12
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  • Some of each species were artificially infected with yellow head virus (YHV) by injection and then monitored by RT-PCR and by immunohistochemistry using monoclonal antibodies specific to 116 kDa, 64 kDa, and 20 kDa proteins of YHV.
  • Using immunohistochemistry and RT-PCR, a small proportion of M. lanchesteri showed very mild YHV infections at Day 3 but no infections at Days 10 and 30.
  • The evidence suggested that M. sintangense, P. styliferus and P. serrifer are susceptible to YHV and carry it for some time.
  • [MeSH-minor] Animals. Antibodies, Monoclonal. Aquaculture / methods. DNA Primers. Immunohistochemistry. Reverse Transcriptase Polymerase Chain Reaction. Thailand. Time Factors

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  • (PMID = 15900682.001).
  • [ISSN] 0177-5103
  • [Journal-full-title] Diseases of aquatic organisms
  • [ISO-abbreviation] Dis. Aquat. Org.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / DNA Primers
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58. Mazloom A, Wolff JE, Paulino AC: The impact of radiotherapy fields in the treatment of patients with choroid plexus carcinoma. Int J Radiat Oncol Biol Phys; 2010 Sep 1;78(1):79-84
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  • PURPOSE: To perform a comprehensive literature review and analysis of cases dealing with choroid plexus carcinoma (CPC) to determine the optimal radiotherapy (RT) treatment field.
  • METHODS AND MATERIALS: A PubMed search of English language articles from 1979 to 2008 was performed, yielding 33 articles with 56 patients who had available data regarding RT treatment field.
  • The RT treatment volume was the craniospinal axis in 38 (68%), whole brain in 9 (16%), and tumor/tumor bed in 9 (16%).
  • The 5-year PFS for patients who had CSI vs. whole brain and tumor/tumor bed RT were 44.2% and 15.3%.
  • For the 19 patients who relapsed, 9 (47%) had a recurrence in the RT field, 6 (32%) had a recurrence outside the RT field, and 4 (21%) had a recurrence inside and outside the irradiated field.
  • CONCLUSION: Patients with CPC who received CSI had better PFS compared with those receiving less than CSI.
  • [MeSH-minor] Adolescent. Adult. Child. Child, Preschool. Cranial Irradiation / methods. Disease-Free Survival. Humans. Infant. Middle Aged. Neoplasm Recurrence, Local. Survival Rate. Young Adult

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  • [Copyright] Copyright (c) 2010 Elsevier Inc. All rights reserved.
  • (PMID = 20004534.001).
  • [ISSN] 1879-355X
  • [Journal-full-title] International journal of radiation oncology, biology, physics
  • [ISO-abbreviation] Int. J. Radiat. Oncol. Biol. Phys.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] United States
  • [Number-of-references] 50
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59. Sanghani M, Balk E, Cady B, Wazer D: Predicting the risk of local recurrence in patients with breast cancer: an approach to a new computer-based predictive tool. Am J Clin Oncol; 2007 Oct;30(5):473-80
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  • , which integrates prognostic factors for local recurrence (LR) into a model to predict the 10-year risk of LR after breast conserving surgery (BCS) with or without radiation therapy (RT) with the goal of assisting with patient counseling and medical decision-making.
  • METHODS: All available randomized trials of BCS alone versus BCS plus RT, meta-analyses, and institutional reports were reviewed to identify the principal prognostic factors for LR after breast-conserving therapy.
  • Patient age, margin status, lymphovascular invasion (LVI), tumor size, tumor grade, use of chemotherapy, and use of hormonal therapy were found to consistently and significantly impact LR across multiple studies.
  • These RR ratios were entered into a mathematical model with the 10-year baseline rates of recurrence with and without RT, 7% and 24%, respectively, to predict patient-specific LR risk.
  • RESULTS: Individual data entered into this computer model with regards to patient age, margin status, LVI, tumor size, tumor grade, use of chemotherapy, and use of hormonal therapy will generate patient-specific predicted 10-year LR risk with and without RT.
  • A graphic representation of the relative risk reduction with RT will also be displayed alongside the numerical display.
  • is a first attempt at a computer model incorporating LR prognostic factors in an evidence-based fashion to predict individual LR risk and the potential additional benefit from RT.
  • [MeSH-major] Breast Neoplasms / pathology. Breast Neoplasms / surgery. Neoplasm Recurrence, Local / epidemiology
  • [MeSH-minor] Adult. Age Factors. Aged. Combined Modality Therapy. Computer Simulation. Female. Humans. Internet. Mastectomy / methods. Meta-Analysis as Topic. Middle Aged. Predictive Value of Tests. Prognosis. Randomized Controlled Trials as Topic. Risk. Risk Factors

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  • (PMID = 17921706.001).
  • [ISSN] 1537-453X
  • [Journal-full-title] American journal of clinical oncology
  • [ISO-abbreviation] Am. J. Clin. Oncol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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60. Sacheck JM, Hyatt JP, Raffaello A, Jagoe RT, Roy RR, Edgerton VR, Lecker SH, Goldberg AL: Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases. FASEB J; 2007 Jan;21(1):140-55
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  • [Title] Rapid disuse and denervation atrophy involve transcriptional changes similar to those of muscle wasting during systemic diseases.
  • To determine whether this transcriptional program also functions during atrophy resulting from loss of contractile activity and whether atrogene expression correlates with the rate of muscle weight loss, we used cDNA microarrays and RT-polymerase chain reaction to analyze changes in mRNA from rat gastrocnemius during disuse atrophy induced by denervation or spinal cord isolation.

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  • (PMID = 17116744.001).
  • [ISSN] 1530-6860
  • [Journal-full-title] FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • [ISO-abbreviation] FASEB J.
  • [Language] eng
  • [Grant] United States / NIAMS NIH HHS / AR / R01 AR055255-02; United States / NIAMS NIH HHS / AR / F32 AR048517; United States / NIAMS NIH HHS / AR / R01 AR055255; United States / NINDS NIH HHS / NS / NS16333; United States / NIDDK NIH HHS / DK / DK6230701
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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61. Yin GL, Yang JS, Cao JX, Yang WJ: Molecular cloning and characterization of FGLamide allatostatin gene from the prawn, Macrobrachium rosenbergii. Peptides; 2006 Jun;27(6):1241-50
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  • In this study, we used a combination of a semi-nested polymerase chain reaction (PCR) and screening of a central nervous system cDNA library of Macrobrachium rosenbergii to isolate and sequence a cDNA clone (2885 bp) encoding a 701 amino acid FGLamide allatostatin precursor polypeptide.
  • Reverse transcription-polymerase chain reaction (RT-PCR) analysis demonstrated that the gene was expressed in the brain, gut, thoracic and abdominal ganglia, but not in the heart, muscle, ovary, gill, or hepatopancreas.
  • Furthermore, developmentally-dependent expression of the gene was observed in the brain and thoracic ganglia of the prawn by using semi-quantitative RT-PCR analysis.
  • [MeSH-minor] Amino Acid Sequence. Animals. Base Sequence. Central Nervous System / metabolism. Cloning, Molecular. Crustacea. Female. Insects. Male. Molecular Sequence Data. Nucleic Acid Hybridization. Reverse Transcriptase Polymerase Chain Reaction. Tissue Distribution

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  • (PMID = 16376458.001).
  • [ISSN] 0196-9781
  • [Journal-full-title] Peptides
  • [ISO-abbreviation] Peptides
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Neuropeptides; 0 / Peptides; 110119-33-0 / allatostatin
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62. Xiao L, Wei YZ, Xia F, Liu SW: [Construction of the fusion gene of Hsp65 of Mycobacterium tuberculosis and EGFP and preparation of dendritic cell vaccine against tuberculosis]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2005 Jan;21(1):13-6
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  • The recombinant plasmid pEGHsp65 was subsequently transfected into Hela cells, and the transfection rate was determined under confocal laser scanning fluorescence microscope at different times.
  • RT-PCR was used to detect the expression of Hsp65 mRNA in Hela cells.
  • Expression of Hsp65 mRNA was detected in Hela cells by RT-PCR.

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  • (PMID = 15629074.001).
  • [ISSN] 1007-8738
  • [Journal-full-title] Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
  • [ISO-abbreviation] Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / Bacterial Vaccines; 0 / Chaperonin 60; 0 / DNA, Recombinant; 0 / enhanced green fluorescent protein; 0 / heat-shock protein 65, Mycobacterium; 147336-22-9 / Green Fluorescent Proteins; EC 3.1.21.- / DNA Restriction Enzymes; EC 3.6.1.- / Chaperonins
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63. Welzel G, Fleckenstein K, Mai SK, Hermann B, Kraus-Tiefenbacher U, Wenz F: Acute neurocognitive impairment during cranial radiation therapy in patients with intracranial tumors. Strahlenther Onkol; 2008 Dec;184(12):647-54
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  • [Title] Acute neurocognitive impairment during cranial radiation therapy in patients with intracranial tumors.
  • BACKGROUND AND PURPOSE: The objective of the current study was to evaluate the acute effects of cranial radiation therapy (CNS-RT) using different radiation doses (0, 1.8, 2, 3, >or=20 Gy) on cognitive function with special emphasis on memory.
  • We assessed patients with and without intracranial tumors to distinguish between direct and indirect radiation effects on brain tissue.
  • MATERIALS AND METHODS: Eighty-two patients were evaluated with neuropsychological testing before and acutely after radiotherapy (RT).
  • Sixty-four patients received RT to the brain (55 with, 9 without intracranial tumor).
  • Eighteen patients treated with RT to the breast served as controls.
  • RESULTS: Patients with intracranial tumor demonstrated attention (19-38th percentile) and verbal memory scores (34-46th percentile) below the population average at baseline.
  • The average Verbal Memory score was significantly different between patients with intracranial tumor and controls both at baseline (38th vs. 58th percentile) and after irradiation (27th vs. 52th percentile).
  • Radiation dose-related deficits were seen for working memory performance in patients with intracranial tumor.
  • Patients with intracranial tumor show a deterioration of almost all memory functions with a dose-dependent impairment in working memory.
  • Patients with preexisting peritumoral brain edema show the strongest deterioration.
  • [MeSH-major] Attention / radiation effects. Brain / radiation effects. Brain Neoplasms / radiotherapy. Cognition / radiation effects. Cranial Irradiation / adverse effects. Memory / radiation effects. Radiotherapy / adverse effects
  • [MeSH-minor] Adult. Aged. Brain Edema / complications. Data Interpretation, Statistical. Female. Humans. Male. Middle Aged. Neuropsychological Tests. Radiotherapy Dosage

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  • (PMID = 19107345.001).
  • [ISSN] 0179-7158
  • [Journal-full-title] Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]
  • [ISO-abbreviation] Strahlenther Onkol
  • [Language] eng
  • [Publication-type] Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
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64. Allen TL, Jolley SJ, Cooley VJ, Winn RT, Harrison JD, Price RR, Rich JC: The epidemiology of illness and injury at the alpine venues during the Salt Lake City 2002 Winter Olympic Games. J Emerg Med; 2006 Feb;30(2):197-202
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  • Over the 19 days of the Olympiad, a total of 410,160 spectators and 3,961 competitive athletes attended the three venues.

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  • (PMID = 16567258.001).
  • [ISSN] 0736-4679
  • [Journal-full-title] The Journal of emergency medicine
  • [ISO-abbreviation] J Emerg Med
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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65. Laskar S, Muckaden MA, Bahl G, de S, Nair R, Gupta S, Bakshi A, Prabhash K, Maru D, Gujral S, Parikh P, Shrivastava SK, Dinshaw KA: Primary non-Hodgkin's lymphoma of the nasopharynx: prognostic factors and outcome of 113 Indian patients. Leuk Lymphoma; 2006 Oct;47(10):2132-9
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  • [Title] Primary non-Hodgkin's lymphoma of the nasopharynx: prognostic factors and outcome of 113 Indian patients.
  • This single institutional study evaluated the prognostic factors and treatment outcome of 113 Indian patients with primary nasopharyngeal non-Hodgkin's lymphoma.
  • Treatment comprised of a combination of chemotherapy (CTh) and radiotherapy (RT) in the majority of the patients (76%).
  • Multivariate analysis showed that; age > 30 years [hazard ratio (HR) = 6.59, 95% confidence interval (CI) = 2.59 - 16.7, P < 0.0001], WHO performance score > or = 2 (HR = 2.34, 95% CI = 1.01 - 5.46, P = 0.050), T-cell lymphomas (HR = 2.81, 95% CI = 1.14 - 6.96, P < 0.001) and the presence of B symptoms (HR = 3.65, 95% CI = 1.77 - 7.53, P = 0.025), had a negative influence on survival.
  • Patients treated with a combination of CTh and RT had a significantly better outcome than those treated with CTh alone (OS: 69%vs. 31%, P < 0.00001).
  • The CR (P = 0.01), DFS (P = 0.01) and OS (P = 0.03) rates were significantly better for patients receiving a RT dose of > or =4500 cGy.
  • HR in the subgroup that received a RT dose of <4500 cGy was 2.51 (95% CI = 1.04 - 6.06).
  • These results suggest that combined modality treatment, comprising of CTh and RT (with an RT dose of > or =4500 cGy), results in satisfactory outcome in patients with this rare neoplasm.
  • [MeSH-minor] Adolescent. Adult. Aged. Child. Combined Modality Therapy / methods. Female. Humans. India. Male. Middle Aged. Prognosis. Time Factors. Treatment Outcome

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  • (PMID = 17071487.001).
  • [ISSN] 1042-8194
  • [Journal-full-title] Leukemia & lymphoma
  • [ISO-abbreviation] Leuk. Lymphoma
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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66. Heinzelmann F, Engelhard M, Ottinger H, Bamberg M, Weinmann M: Nodal follicular lymphoma: the role of radiotherapy for stages I and II. Strahlenther Onkol; 2010 Apr;186(4):191-6
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  • PURPOSE: To clarify the role of external-beam radiotherapy (RT) in patients with stage I and II nodal follicular lymphoma (FL).
  • RESULTS: In patients with stage I and II nodal FL, RT alone with different irradiation techniques (involved-field [IFI]/extended-field [EFI]/total nodal [TNI]/total lymphoid irradiation [TLI]) produces excellent local disease control (approximately 95%) resulting in disease-free survival and overall survival (OS) rates of 37-94% and 40-93% at 5-15 years, respectively.
  • Additional chemotherapy mostly failed to improve treatment results achieved with RT alone.
  • To further improve the curative potential of RT in early-stage FL, novel combined approaches (e.g., RT + immunotherapy with rituximab) are under investigation.
  • [MeSH-minor] Disease-Free Survival. Humans. Lymphatic Irradiation / methods. Neoplasm Recurrence, Local / pathology. Neoplasm Recurrence, Local / radiotherapy. Neoplasm Staging. Prognosis. Radiotherapy Dosage. Risk Factors. Whole-Body Irradiation / methods

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  • (PMID = 20354662.001).
  • [ISSN] 1439-099X
  • [Journal-full-title] Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al]
  • [ISO-abbreviation] Strahlenther Onkol
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] Germany
  • [Number-of-references] 60
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67. Choi GB, Shin JH, Song HY, Lee YS, Cho YK, Bae JI, Kim JH, Jeong YH, Park MH: Fluoroscopically guided balloon dilation for patients with esophageal stricture after radiation treatment. J Vasc Interv Radiol; 2005 Dec;16(12):1705-10
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  • PURPOSE: To evaluate the safety and clinical effectiveness of fluoroscopically guided balloon dilation in patients with esophageal stricture after radiation therapy (RT).
  • MATERIALS AND METHODS: From April 1993 through December 2004, fluoroscopically guided balloon dilation was performed in 15 patients with esophageal strictures secondary to previous RT.
  • Technical success, clinical success, recurrence of dysphagia, primary and secondary patency rates, and complications related to the procedure were retrospectively evaluated.
  • Dysphagia recurred 2-128 days (mean, 67.2 d) after the first balloon dilation in six of the 13 patients (46%), who underwent further balloon dilation and/or stent placement.
  • The primary and secondary patency rates at 1, 3, and 6 months were 86%, 68%, and 47% and 100%, 92%, and 62%, respectively.
  • CONCLUSION: Fluoroscopically guided balloon dilation for esophageal stricture after RT can be safe and effective.

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  • (PMID = 16371539.001).
  • [ISSN] 1051-0443
  • [Journal-full-title] Journal of vascular and interventional radiology : JVIR
  • [ISO-abbreviation] J Vasc Interv Radiol
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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68. Bessong PO, Obi CL, Andréola ML, Rojas LB, Pouységu L, Igumbor E, Meyer JJ, Quideau S, Litvak S: Evaluation of selected South African medicinal plants for inhibitory properties against human immunodeficiency virus type 1 reverse transcriptase and integrase. J Ethnopharmacol; 2005 May 13;99(1):83-91
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  • Seventeen aqueous and methanol extracts from nine South African medicinal plants, ethnobotanically selected, were screened for inhibitory properties against HIV-1 reverse transcriptase (RT).
  • The strongest inhibition against the RNA-dependent-DNA polymerase (RDDP) activity of RT was observed with the methanol extract of the stem-bark of Peltophorum africanum Sond. (Fabaceae) (IC(50) 3.5 microg/ml), while the methanol extract of the roots of Combretum molle R.Br. ex G.
  • The gallotannin inhibited the RDDP and RNase H functions of RT with IC(50) values of 6.0 and 5.0 microM, respectively, and abolished the 3'-end processing activity of IN at 100 microM.
  • Catechin showed no effect on RT but had a moderate activity on HIV-1 IN.
  • [MeSH-minor] Cell Survival / drug effects. Combretum / chemistry. DNA, Viral / drug effects. Ethanol. Humans. Medicine, African Traditional. Plant Extracts / chemistry. Plant Extracts / pharmacology. Solvents. South Africa. Tumor Cells, Cultured. Water

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  • (PMID = 15848024.001).
  • [ISSN] 0378-8741
  • [Journal-full-title] Journal of ethnopharmacology
  • [ISO-abbreviation] J Ethnopharmacol
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Anti-HIV Agents; 0 / DNA, Viral; 0 / HIV Integrase Inhibitors; 0 / Plant Extracts; 0 / Reverse Transcriptase Inhibitors; 0 / Solvents; 059QF0KO0R / Water; 3K9958V90M / Ethanol; EC 2.7.7.- / HIV Integrase; EC 2.7.7.49 / HIV Reverse Transcriptase
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69. Schweighofer B, Testori J, Sturtzel C, Sattler S, Mayer H, Wagner O, Bilban M, Hofer E: The VEGF-induced transcriptional response comprises gene clusters at the crossroad of angiogenesis and inflammation. Thromb Haemost; 2009 Sep;102(3):544-54
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  • As shown by real-time RT-PCR for selected genes, VEGF-induction was mostly mediated by VEGF receptor-2 and the capacity of VEGF-A to induce genes in common with IL-1 largely depended on activation of the calcineurin/NFAT pathway, since cyclosporin A inhibited this induction.
  • [MeSH-major] Gene Expression Regulation. Inflammation. Neovascularization, Pathologic. Transcription, Genetic. Vascular Endothelial Growth Factor A / metabolism
  • [MeSH-minor] Calcineurin / metabolism. Cyclosporine / metabolism. Endothelial Cells / cytology. Humans. Interleukin-1 / metabolism. Models, Genetic. Multigene Family. Oligonucleotide Array Sequence Analysis. Reverse Transcriptase Polymerase Chain Reaction. Vascular Endothelial Growth Factor Receptor-2 / metabolism

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  • (PMID = 19718476.001).
  • [ISSN] 0340-6245
  • [Journal-full-title] Thrombosis and haemostasis
  • [ISO-abbreviation] Thromb. Haemost.
  • [Language] eng
  • [Grant] Austria / Austrian Science Fund FWF / / S 9403
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Interleukin-1; 0 / Vascular Endothelial Growth Factor A; 83HN0GTJ6D / Cyclosporine; EC 2.7.10.1 / Vascular Endothelial Growth Factor Receptor-2; EC 3.1.3.16 / Calcineurin
  • [Other-IDs] NLM/ PMC2886966; NLM/ UKMS30254
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70. Petch LA, Hoffman IF, Jere CS, Kazembe PN, Martinson FE, Chilongozi D, Fiscus SA, Cohen MS: Genotypic analysis of the protease and reverse transcriptase of HIV type 1 subtype C isolates from antiretroviral drug-naive adults in Malawi. AIDS Res Hum Retroviruses; 2005 Sep;21(9):799-805
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  • The protease (PR) and reverse transcriptase (RT) regions of HIV-1 isolates from 21 antiretroviral (ARV)-naive Malawian adults were sequenced and analyzed to determine the prevalence of drug resistance-associated mutations in this population.
  • No major mutations associated with resistance to PR inhibitors (PIs), nucleoside RT inhibitors (NRTIs), or nonnucleoside RT inhibitors (NNRTIs) were found.
  • In contrast, accessory mutations were found in the protease region at positions 10, 20, 36, 63, 77, and 93, and in the RT region at positions 118, 211, and 214.
  • [MeSH-minor] Adult. Amino Acid Sequence. Consensus Sequence. Drug Resistance, Viral / genetics. Humans. Malawi. Molecular Sequence Data. Mutation. Phylogeny. Sequence Alignment

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  • (PMID = 16218804.001).
  • [ISSN] 0889-2229
  • [Journal-full-title] AIDS research and human retroviruses
  • [ISO-abbreviation] AIDS Res. Hum. Retroviruses
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ AY860456/ AY860457/ AY860458/ AY860459/ AY860460/ AY860461/ AY860462/ AY860463/ AY860464/ AY860465/ AY860466/ AY860467/ AY860468/ AY860469/ AY860470/ AY860471/ AY860472/ AY860473/ AY860474/ AY860475/ AY860476/ AY860477/ AY860478/ AY860479/ AY860480/ AY860481/ AY860482/ AY860483/ AY860484/ AY860485/ AY860486/ AY860487/ AY860488/ AY860489/ AY860490/ AY860491/ AY860492/ AY860493/ AY860494/ AY860495/ AY860496/ AY860497
  • [Grant] United States / NIAID NIH HHS / AI / 5 U01 AI048005-04; United States / FIC NIH HHS / TW / D43 TW01039-05S1; United States / NIAID NIH HHS / AI / P30 AI50410
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] EC 2.7.7.49 / HIV Reverse Transcriptase; EC 3.4.23.- / HIV Protease
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71. Liang SH, Li J, Al-beit M, Zhang J, Ma D, Lu X: [Screening and identification of potential miRNA involved in ovarian cancer invasion and metastasis]. Zhonghua Zhong Liu Za Zhi; 2010 Sep;32(9):650-4
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  • Real-time RT-PCR was used to confirm the results of microarray and for expanding detection in another paired EOC cell lines (HO-8910 and HO-8910PM).
  • Meanwhile, real-time RT-PCR confirmation and statistic analysis showed that let-7f and miR-22 expressions were significantly different between ovarian cancer cell lines with various invasive and metastatic capacity (P < 0.05).
  • It suggests that they are potential tumor suppressor genes.
  • [MeSH-minor] Cell Line, Tumor. Female. Gene Expression Profiling. Humans. Microarray Analysis / methods. Neoplasm Invasiveness. Neoplasm Metastasis

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  • (PMID = 21122376.001).
  • [ISSN] 0253-3766
  • [Journal-full-title] Zhonghua zhong liu za zhi [Chinese journal of oncology]
  • [ISO-abbreviation] Zhonghua Zhong Liu Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / MIRN22 microRNA, human; 0 / MicroRNAs; 0 / mirnlet7 microRNA, human
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72. Wang J, Tuo H, Wang R, Li W, Feng Z, Chen J, Wang D: The expression of human brain vascular smooth muscle cell AT receptor after the UL83 gene of HCMV inhibition by small interfering RNAs. Neurol Res; 2008 Nov;30(9):903-9
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  • [Title] The expression of human brain vascular smooth muscle cell AT receptor after the UL83 gene of HCMV inhibition by small interfering RNAs.
  • METHODS: Cell model of RNA interference (RNAi) HCMV UL83 gene was set up by transfection RNAi into HCMV infected human cerebral artery vascular smooth muscle cells cultured in vitro by application of transfection technique and adoption of RT-PCR, etc.
  • Effect of HCMV on gene expression of AT1 and AT2 receptors in human cerebral artery vascular smooth muscle cells cultured in vitro under the action of Ganciclovir (GCV), AT1 and AT2 receptor antagonists by immunofluorescence technique, RT-PCR and protein electrophoresis.
  • AT1 and AT2 receptor antagonists and GCV, under certain condition, could inhibit the activation on AT1 and AT2 receptor in human cerebral artery vascular smooth muscle cells induced by HCMV infection.
  • CONCLUSION: Cell model of HCMV infected UL83 silence gene was successfully set up in human cerebral artery vascular smooth muscle cells cultured in vitro.
  • [MeSH-major] Cytomegalovirus / genetics. Muscle, Smooth, Vascular / metabolism. Phosphoproteins / genetics. RNA Interference. Receptors, Angiotensin / genetics. Viral Matrix Proteins / genetics

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  • (PMID = 18534055.001).
  • [ISSN] 0161-6412
  • [Journal-full-title] Neurological research
  • [ISO-abbreviation] Neurol. Res.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Phosphoproteins; 0 / RNA, Messenger; 0 / RNA, Small Interfering; 0 / Receptor, Angiotensin, Type 1; 0 / Receptor, Angiotensin, Type 2; 0 / Receptors, Angiotensin; 0 / Viral Matrix Proteins; 0 / cytomegalovirus matrix protein 65kDa
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73. Shivers S, Jakub J, Pendas S, Reintgen D: Molecular staging for patients with malignant melanoma. Expert Rev Anticancer Ther; 2007 Nov;7(11):1665-74
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  • [Title] Molecular staging for patients with malignant melanoma.
  • With newer reverse transcriptase-PCR (RT-PCR)-based assays, sensitivities reported are as high as identifying one cancer cell in a background of a million normal cells.
  • Although this sensitivity is 100-times what the human eye can differentiate under the microscope, the new challenge becomes determining the relevance of this low-volume disease in the regional basin, in particular, the sentinel lymph node (SLN).
  • Patients with melanomas greater than 0.75 mm in tumor thickness participated in a research study that examined their SLNs with routine histology, immunhistochemical staining and a RT-PCR assay based on the tyrosinase probe.
  • A total of 311 patients were involved in the study and patients whose SLN were negative from all three assays for metastatic disease had a good survival, with a 92% disease-free survival (DFS) and a 97% overall survival (OS) regardless of the tumor thickness or the ulceration status of the primary melanoma.
  • Patients upstaged with the RT-PCR assay had a significantly decreased DFS and OS compared with patients who were SLN negative.
  • Patients who had enough tumor burden in the SLN that allowed their metastatic disease to be identified with routine histology had a 48% recurrence rate at 5 years.
  • [MeSH-minor] Biomarkers, Tumor / analysis. Chemotherapy, Adjuvant. Clinical Trials as Topic. Humans. Lymphatic Metastasis. Neoplasm Staging. Nerve Growth Factors / analysis. Prognosis. RNA, Neoplasm / genetics. Radionuclide Imaging. Reverse Transcriptase Polymerase Chain Reaction. S100 Calcium Binding Protein beta Subunit. S100 Proteins / analysis. Sensitivity and Specificity. Sentinel Lymph Node Biopsy

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  • (PMID = 18020932.001).
  • [ISSN] 1744-8328
  • [Journal-full-title] Expert review of anticancer therapy
  • [ISO-abbreviation] Expert Rev Anticancer Ther
  • [Language] eng
  • [Publication-type] Journal Article; Meta-Analysis
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Biomarkers, Tumor; 0 / Nerve Growth Factors; 0 / RNA, Neoplasm; 0 / S100 Calcium Binding Protein beta Subunit; 0 / S100 Proteins
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74. Ikema K, Matsumoto K, Inomata Y, Komohara Y, Miyajima S, Takeya M, Tanihara H: Induction of matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs correlates with outcome of acute experimental pseudomonal keratitis. Exp Eye Res; 2006 Dec;83(6):1396-404
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  • Pseudomonal keratitis was induced in New Zealand white rabbits, and macroscopic and microscopic examinations were performed at appropriate time points (3, 9, 12, 18, 24, 72 h).
  • Expressions and sources of MMP-2, 9, and TIMP-1, 2 were determined using immunohistochemistry, gelatin zymography, ELISA, and RT-PCR.
  • MMP-2 was constitutively expressed in keratocytes, and its expression was not apparently enhanced after pseudomonal infection as evidenced by zymography, immunostaining, and RT-PCR.
  • Although TIMP-2 was constitutively expressed as seen by immunostaining and RT-PCR, its concentration was below detection limits during the experiments.

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  • (PMID = 16968651.001).
  • [ISSN] 0014-4835
  • [Journal-full-title] Experimental eye research
  • [ISO-abbreviation] Exp. Eye Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Messenger; 0 / Tissue Inhibitor of Metalloproteinase-1; 0 / Tissue Inhibitor of Metalloproteinases; 127497-59-0 / Tissue Inhibitor of Metalloproteinase-2; EC 3.4.24.- / Matrix Metalloproteinases; EC 3.4.24.24 / Matrix Metalloproteinase 2; EC 3.4.24.35 / Matrix Metalloproteinase 9
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75. Dzialowski I, Hill MD, Coutts SB, Demchuk AM, Kent DM, Wunderlich O, von Kummer R: Extent of early ischemic changes on computed tomography (CT) before thrombolysis: prognostic value of the Alberta Stroke Program Early CT Score in ECASS II. Stroke; 2006 Apr;37(4):973-8
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  • The Alberta Stroke Program Early CT Score (ASPECTS) semiquantitatively assesses EICs within the middle cerebral artery territory using a10-point grading system.
  • METHODS: Data from the European-Australian Acute Stroke Study (ECASS) II study were used in which 800 patients were randomized to recombinant tissue plasminogen activator (rt-PA) or placebo within 6 hours of symptom onset.
  • We performed a multivariable logistic regression analysis and assessed for an interaction between rt-PA treatment and baseline ASPECTS score.
  • CONCLUSIONS: In ECASS II, the effect of rt-PA on functional outcome is not influenced by baseline ASPECTS.
  • [MeSH-major] Brain Ischemia / drug therapy. Brain Ischemia / radiography. Fibrinolytic Agents / therapeutic use. Tissue Plasminogen Activator / therapeutic use. Tomography, X-Ray Computed
  • [MeSH-minor] Aged. Cerebral Hemorrhage / chemically induced. Cerebral Hemorrhage / epidemiology. Female. Humans. Incidence. Injections, Intravenous. Male. Middle Aged. Middle Cerebral Artery / radiography. Predictive Value of Tests. Prognosis. Randomized Controlled Trials as Topic. Recombinant Proteins / administration & dosage. Recombinant Proteins / therapeutic use. Time Factors

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  • (PMID = 16497977.001).
  • [ISSN] 1524-4628
  • [Journal-full-title] Stroke; a journal of cerebral circulation
  • [ISO-abbreviation] Stroke
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Fibrinolytic Agents; 0 / Recombinant Proteins; EC 3.4.21.68 / Tissue Plasminogen Activator
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76. Schlueter SR, Carnes DL, Cochran DL: In vitro effects of enamel matrix derivative on microvascular cells. J Periodontol; 2007 Jan;78(1):141-51
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  • BACKGROUND: Periodontal regeneration requires a coordinated series of events that includes not only the recruitment of periodontal ligament (PDL)-specific cells, but vascular cells as well.
  • The mechanisms of action of enamel matrix derivative (EMD) are poorly understood, and its effects on vascular cells are unknown.
  • Effects of EMD on PDL expression of vascular endothelial cell (VEGF) types (A, B, C, and D) and isoforms were determined using reverse transcription-polymerase chain reaction (RT-PCR).
  • RT-PCR results indicated that PDL cells expressed VEGF-A, -B, and -C and multiple isoforms of VEGF-A, including VEGF(121), VEGF(165), and VEGF(189), whether or not EMD was present in the culture media.

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  • (PMID = 17199551.001).
  • [ISSN] 0022-3492
  • [Journal-full-title] Journal of periodontology
  • [ISO-abbreviation] J. Periodontol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Dental Enamel Proteins; 0 / Growth Substances; 0 / enamel matrix proteins
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77. Lei RC, Jian XC, Qin ZQ: [Change of the expression level of Th1 and Th2 cytokines around the xenogeneic cancellous bone scaffold after transplantation: an experiment in rabbits]. Zhonghua Zheng Xing Wai Ke Za Zhi; 2007 Nov;23(6):507-10
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  • The histological investigation and the expression of Th1 (such as IL-2, IFN-gamma) and Th2 (such as IL-4, IL-10) mRNA were measured by HE, RT-PCR respectively in 1,2,4,8,12 week postoperation.
  • RT-PCR revealed: The mRNA expression level of Th1 cytokines such as IL-2, IFN-gamma was high in 1 week and began to fall since 2 week.

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  • (PMID = 18269028.001).
  • [ISSN] 1009-4598
  • [Journal-full-title] Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery
  • [ISO-abbreviation] Zhonghua Zheng Xing Wai Ke Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Cytokines; 0 / RNA, Messenger
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78. Yu L, Ma H, Yang SJ: [Expression of HSP70 in human umbilical vein endothelial cells induced by Hantavirus]. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi; 2005 Jan;21(1):9-12
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  • METHODS: The expression of HSP70 was detected by immunocytochemical staining, in situ nucleic acid hybridization and RT-PCR.
  • RT-PCR showed that the level of HSP70 mRNA increased rapidly and kept at a high level in comparison with the normal control (P<0.05).

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  • (PMID = 15629073.001).
  • [ISSN] 1007-8738
  • [Journal-full-title] Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
  • [ISO-abbreviation] Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / HSP70 Heat-Shock Proteins; 0 / RNA, Messenger
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79. Delviks-Frankenberry KA, Nikolenko GN, Barr R, Pathak VK: Mutations in human immunodeficiency virus type 1 RNase H primer grip enhance 3'-azido-3'-deoxythymidine resistance. J Virol; 2007 Jul;81(13):6837-45
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  • [Title] Mutations in human immunodeficiency virus type 1 RNase H primer grip enhance 3'-azido-3'-deoxythymidine resistance.
  • We recently observed that mutations in the human immunodeficiency type 1 (HIV-1) reverse transcriptase (RT) connection domain significantly increase 3'-azido-3'-deoxythymidine (AZT) resistance up to 536 times over wild-type (WT) RT in the presence of thymidine analog resistance mutations (TAMs).
  • These mutations also decreased RT template switching, suggesting that they altered the balance between nucleotide excision and template RNA degradation, which in turn increased AZT resistance.
  • To test the hypothesis that connection domain mutations enhanced AZT resistance by influencing the RNase H primer grip, we determined the effects of alanine substitutions in RNase H primer grip residues on nucleoside RT inhibitor resistance in the context of a WT, TAM-containing, or K65R-containing polymerase domain.
  • Ten of the 11 RNase H primer grip mutations increased AZT resistance 20 to 243 times above WT levels in the context of a TAM-containing polymerase domain.
  • These results demonstrate that mutations in the RNase H primer grip region can significantly enhance AZT resistance and support the hypothesis that mutations in the connection and RNase H domains can increase resistance by altering the RNase H primer grip region, changing interactions between RT and the template-primer complex and/or shifting the balance between the polymerase and RNase H activities.

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  • (PMID = 17428874.001).
  • [ISSN] 0022-538X
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] ENG
  • [Grant] United States / Intramural NIH HHS / /
  • [Publication-type] Journal Article; Research Support, N.I.H., Intramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / RNA primers; 0 / Reverse Transcriptase Inhibitors; 4B9XT59T7S / Zidovudine; 63231-63-0 / RNA; EC 2.7.7.49 / HIV Reverse Transcriptase; EC 3.1.26.4 / Ribonuclease H
  • [Other-IDs] NLM/ PMC1933283
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80. Ertan Y, Sezak M, Turhan T, Kantar M, Erşahin Y, Mutluer S, Vergin C, Oniz H, Akalin T: Atypical teratoid/rhabdoid tumor of the central nervous system: clinicopathologic and immunohistochemical features of four cases. Childs Nerv Syst; 2009 Jun;25(6):707-11
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  • [Title] Atypical teratoid/rhabdoid tumor of the central nervous system: clinicopathologic and immunohistochemical features of four cases.
  • BACKGROUND: Atypical teratoid/rhabdoid tumor (AT/RT) is a rare aggressive infantile neoplasm of uncertain origin.
  • This study was performed to assess the clinicopathologic and immunohistochemical features of four AT/RT cases.
  • Tumors were located in the cerebellum (two cases), frontoparietal lobe (one case), and third ventricle (one case).
  • Histopathologically, the tumors were composed of rhabdoid cells and undifferentiated small cells mixed with epithelial or mesenchymal components.
  • However, one of the tumors was composed predominantly of a mesenchymal component mimicking a sarcoma.
  • All of the patients died within a mean of 14 months due to tumor progression despite the chemotherapy.
  • In conclusion, AT/RTs are aggressive tumors.
  • [MeSH-major] Brain Neoplasms / pathology. Rhabdoid Tumor / pathology. Teratoma / pathology
  • [MeSH-minor] Actins / analysis. Brain / pathology. Brain Chemistry. Child. Child, Preschool. Chromosomal Proteins, Non-Histone / analysis. DNA-Binding Proteins / analysis. Desmin / analysis. Diagnosis, Differential. Female. Glial Fibrillary Acidic Protein / analysis. Humans. Immunohistochemistry. Infant. Keratins / analysis. Male. Mucin-1 / analysis. S100 Proteins / analysis. SMARCB1 Protein. Synaptophysin / analysis. Transcription Factors / analysis. Vimentin / analysis

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  • [CommentIn] Childs Nerv Syst. 2009 Nov;25(11):1387; author reply 1389 [19636570.001]
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  • (PMID = 19212771.001).
  • [ISSN] 1433-0350
  • [Journal-full-title] Child's nervous system : ChNS : official journal of the International Society for Pediatric Neurosurgery
  • [ISO-abbreviation] Childs Nerv Syst
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Actins; 0 / Chromosomal Proteins, Non-Histone; 0 / DNA-Binding Proteins; 0 / Desmin; 0 / Glial Fibrillary Acidic Protein; 0 / Mucin-1; 0 / S100 Proteins; 0 / SMARCB1 Protein; 0 / SMARCB1 protein, human; 0 / Synaptophysin; 0 / Transcription Factors; 0 / Vimentin; 68238-35-7 / Keratins
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81. Manini T, Marko M, VanArnam T, Cook S, Fernhall B, Burke J, Ploutz-Snyder L: Efficacy of resistance and task-specific exercise in older adults who modify tasks of everyday life. J Gerontol A Biol Sci Med Sci; 2007 Jun;62(6):616-23
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  • [Title] Efficacy of resistance and task-specific exercise in older adults who modify tasks of everyday life.
  • BACKGROUND: The purpose of this study was to determine the efficacy of 10 weeks of resistance (RT), functional (FT), or functional plus resistance (FRT) training in older adults who modify tasks of everyday life and are at risk for subsequent disability.
  • The primary outcome of the study was the number of task modifications and timed performance on eight tasks of daily life.
  • Secondary outcomes included knee and elbow strength (extension and flexion), body composition, self-reported physical function, single-leg balance time, walking speed, and time to vacuum a carpet.
  • The RT group performed progressive intensity training, and the FT group performed task-specific exercises 2 days per week.
  • All three training groups reduced the need to modify tasks of everyday life (RT: 21%, FRT: 26%, and FT: 28%) and improved self-rated function and time to vacuum a carpet.
  • Individuals who performed FT either 1 or 2 days per week also reduced their timed performance (RT: 2.5% [p = 0.48], FRT: 18.5%, and FT: 23%).
  • Strength gains were primarily found in groups that performed RT either 1 or 2 days per week (RT and FRT).
  • [MeSH-minor] Aged. Body Composition / physiology. Elbow Joint / physiology. Female. Follow-Up Studies. Gait / physiology. Humans. Isometric Contraction / physiology. Knee Joint / physiology. Male. Muscle Strength / physiology. Postural Balance / physiology. Risk Factors. Task Performance and Analysis. Time Factors. Walking / physiology. Weight Lifting

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  • (PMID = 17595417.001).
  • [ISSN] 1079-5006
  • [Journal-full-title] The journals of gerontology. Series A, Biological sciences and medical sciences
  • [ISO-abbreviation] J. Gerontol. A Biol. Sci. Med. Sci.
  • [Language] eng
  • [Grant] United States / NIA NIH HHS / AG / P30 AG028740
  • [Publication-type] Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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82. Rades D, Schild SE: Treatment recommendations for the various subgroups of neurocytomas. J Neurooncol; 2006 May;77(3):305-9
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  • This study was performed to determine the best available treatment for typical and atypical neurocytomas (MIB-1 labeling index >3%, atypical histology) in various age groups ( <or=18 years, >18 years).
  • Patients were treated with complete resection alone (CTR), CTR plus radiotherapy (CTR + RT), incomplete resection alone (ITR), or ITR plus radiotherapy (ITR + RT).
  • Three hundred and fifty-one patients had typical, 87 atypical lesions.
  • Typical lesions were associated with better local control and survival than atypical lesions (P < 0.001).
  • After CTR, outcome was not significantly improved by RT.
  • After ITR, RT improved survival in typical lesions (P = 0.03) and atypical lesions (P = 0.05), not in children (P = 0.16).
  • Doses >54 Gy appeared beneficial after ITR of atypical lesions.
  • In children, <or=50 Gy and >50 Gy were comparable.CTR does not require post-operative RT.
  • Following ITR, RT improves outcome.
  • Atypical lesions require 56-60 Gy.

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  • (PMID = 16575540.001).
  • [ISSN] 0167-594X
  • [Journal-full-title] Journal of neuro-oncology
  • [ISO-abbreviation] J. Neurooncol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] United States
  • [Number-of-references] 22
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83. Taal W, Brandsma D, de Bruin HG, Bromberg JE, Swaak-Kragten AT, Smitt PA, van Es CA, van den Bent MJ: Incidence of early pseudo-progression in a cohort of malignant glioma patients treated with chemoirradiation with temozolomide. Cancer; 2008 Jul 15;113(2):405-10
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  • [Title] Incidence of early pseudo-progression in a cohort of malignant glioma patients treated with chemoirradiation with temozolomide.
  • BACKGROUND: Radiotherapy (RT) plus concomitant and adjuvant temozolomide (TMZ) is now the standard of care for patients with newly diagnosed glioblastoma.
  • The occurrence of pseudo-progression directly after RT is a recognized phenomenon, but to the authors' knowledge its incidence after combined RT/TMZ is unknown.
  • The occurrence of early pseudo-progression was retrospectively assessed in a cohort of malignant glioma patients treated with RT/TMZ.
  • METHODS: The pre-RT and post-RT brain scans from patients treated with RT/TMZ for a malignant glioma were reviewed.
  • Scans were made before the start of RT, 4 weeks after the end of RT, and every 3 months thereafter.
  • In 36 patients (42%) the first follow-up scan 4 weeks after the end of RT indicated disease progression.
  • Six of 18 patients with pseudo-progression and 12 of the 18 patients with real tumor progression developed new clinical signs and symptoms during RT or in the first 4 weeks thereafter.
  • CONCLUSIONS: Up to 50% of malignant glioma patients treated with RT/TMZ and progression immediately after RT develop pseudo-progression.
  • The current study data support the idea to continue TMZ in the case of progressive lesions immediately after RT/TMZ.
  • [MeSH-minor] Adolescent. Adult. Aged. Cohort Studies. Combined Modality Therapy. Disease Progression. Female. Humans. Magnetic Resonance Imaging. Male. Middle Aged. Retrospective Studies. Survival Rate. Time Factors. Treatment Outcome

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  • [Copyright] (c) 2008 American Cancer Society.
  • (PMID = 18484594.001).
  • [ISSN] 0008-543X
  • [Journal-full-title] Cancer
  • [ISO-abbreviation] Cancer
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 7GR28W0FJI / Dacarbazine; 85622-93-1 / temozolomide
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84. Tamm K, Rõõm M, Salumets A, Metsis M: Genes targeted by the estrogen and progesterone receptors in the human endometrial cell lines HEC1A and RL95-2. Reprod Biol Endocrinol; 2009;7:150
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  • Expression analysis by reverse transcriptase (RT)-PCR was used to further investigate hormone dependent mRNA expression regulation of a subset of genes.
  • RT-PCR analysis of 20 genes demonstrated transcriptional changes after estradiol or progesterone treatment of the cell lines.
  • CONCLUSIONS: Combined results from ChIP-qPCR and RT-PCR analysis showed different patterns of steroid hormone receptor occupancy at target genes, corresponding to activation or suppression of gene expression after hormone treatment of HEC1A and RL95-2 cell lines.

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  • (PMID = 20034404.001).
  • [ISSN] 1477-7827
  • [Journal-full-title] Reproductive biology and endocrinology : RB&E
  • [ISO-abbreviation] Reprod. Biol. Endocrinol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Chromatin; 0 / RNA, Messenger; 0 / Receptors, Estrogen; 0 / Receptors, Progesterone
  • [Other-IDs] NLM/ PMC2805670
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85. Held DM, Kissel JD, Thacker SJ, Michalowski D, Saran D, Ji J, Hardy RW, Rossi JJ, Burke DH: Cross-clade inhibition of recombinant human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian immunodeficiency virus SIVcpz reverse transcriptases by RNA pseudoknot aptamers. J Virol; 2007 May;81(10):5375-84
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  • Reverse transcriptase (RT) remains a primary target in therapies directed at human immunodeficiency virus type 1 (HIV-1).
  • RNA aptamers that bind RT from HIV-1 subtype B have been shown to protect human cells from infection and to reduce viral infectivity, but little is known about the sensitivity of the inhibition to amino sequence variations of the RT target.
  • Therefore, we assembled a panel of 10 recombinant RTs from phylogenetically diverse lentiviral isolates (including strains of HIV-1, simian immunodeficiency virus SIVcpz, and HIV-2).
  • All four aptamers potently inhibited RTs from multiple HIV-1 subtypes.
  • For aptamers carrying family 1 pseudoknots, natural resistance was essentially all-or-none and correlated with the identity of the amino acid at position 277.
  • In contrast, natural resistance to aptamers carrying the family 2 pseudoknots was much more heterogeneous, both in degree (gradation of 50% inhibitory concentrations) and in distribution across clades.
  • Site-directed and subunit-specific mutagenesis identified a common R/K polymorphism within the p66 subunit as a primary determinant of resistance to family 1, but not family 2, pseudoknot aptamers.
  • RNA structural diversity therefore translates into a nonoverlapping spectrum of mutations that confer resistance, likely due to differences in atomic-level contacts with RT.

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  • (PMID = 17329328.001).
  • [ISSN] 0022-538X
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] ENG
  • [Databank-accession-numbers] GENBANK/ DQ234256
  • [Grant] United States / NIAID NIH HHS / AI / R21 AI062513; United States / NIAID NIH HHS / AI / AI62513
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Aptamers, Nucleotide; 0 / RNA, Viral; 0 / Recombinant Proteins; 0 / Reverse Transcriptase Inhibitors; EC 2.7.7.49 / HIV Reverse Transcriptase; EC 2.7.7.49 / RNA-Directed DNA Polymerase
  • [Other-IDs] NLM/ PMC1900219
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86. Reinbold JB, Coetzee JF, Hollis LC, Nickell JS, Riegel C, Olson KC, Ganta RR: The efficacy of three chlortetracycline regimens in the treatment of persistent Anaplasma marginale infection. Vet Microbiol; 2010 Sep 28;145(1-2):69-75
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  • Chronic carrier status was established in 21 steers with a Virginia isolate of Anaplasma marginale and confirmed by cELISA and an A. marginale-specific RT-PCR.
  • Blood samples were collected semi-weekly for determining plasma drug concentration by ultrahigh performance liquid chromatography-mass spectrometry/mass spectrometry method and assessment of disease status by both cELISA and RT-PCR.
  • A negative RT-PCR assay result was confirmed in all CTC-treated groups within 49 days of treatment; however, cELISA required an additional 49 to 88 days before similar results.

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  • [Copyright] (c) 2010 Elsevier B.V. All rights reserved.
  • (PMID = 20346598.001).
  • [ISSN] 1873-2542
  • [Journal-full-title] Veterinary microbiology
  • [ISO-abbreviation] Vet. Microbiol.
  • [Language] eng
  • [Grant] United States / NIAID NIH HHS / AI / R01 AI070908; United States / NIAID NIH HHS / AI / AI070908
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] Netherlands
  • [Chemical-registry-number] WCK1KIQ23Q / Chlortetracycline
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87. Nikonova AA, Uspenskaia ES, Lobodanov SA, Oksanich AS, Gorbalenia AE, Claas EC, Foshina EP, Kaira AN, Zverev VV, Faĭzuloev EB: [Use of multiplex PCR method with real-time detection for differential diagnosis of respiratory viral infections]. Zh Mikrobiol Epidemiol Immunobiol; 2009 Jan-Feb;(1):67-70
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  • [Title] [Use of multiplex PCR method with real-time detection for differential diagnosis of respiratory viral infections].
  • Multiplex real-time polymerase chain reaction test-system with fluorescent detection (RT-PCR) for simultaneous identification of main agents of acute respiratory viral infections: influenza A (IAV) and B viruses (IBV), parainfluenza viruses types 1, 2, 3, 4 (PIV 1 - 4), adenoviruses (ADV), respiratory syncitial virus (RSV), rhinoviruses (RV) and enteroviruses (EV), in presence internal positive control (IPC) represented by vaccine strain of rubella virus RA 27/3.
  • Using multiplex RT-PCR method, respiratory viruses were detected in 116 out of 226 clinical samples (nasal swabs) obtained from patients with symptoms of acute respiratory infection: in 68 (58.6%) samples--IBV; in 21 (18.1%)--IAV; in 12 (10.3%) --RV; in 6 (5.2%)--PIV 2; in 4--(3.4%) ADV; in 3 (2.6%)--RSV; in 2 (1.7%)--EV; in 2 (1.7%)--PIV 4; in 1 (0.9%)--PIV 3; in 1 (0.9%)--PIV 1.
  • At the same time samples were tested for the presence of 12 respiratory viruses--IAV, IBV, PIV 1 - 4, RSV, RV, metapneumoviruses, and coronaviruses NL63, 229E and OC43--in the presence of IPC represented by equine arteritis virus using analogous PCR test-system provided by medical center of Leiden university.
  • Results of tests for detection of IAV, IBV, RSV, PIV 1 - 4, and RV, analyzed by both systems, agreed in 94%.
  • Multiplex format of RT-PCR performing significantly reduces time and cost of the test, which make it suitable and effective instrument of epidemiological studies.

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  • (PMID = 19338239.001).
  • [ISSN] 0372-9311
  • [Journal-full-title] Zhurnal mikrobiologii, epidemiologii, i immunobiologii
  • [ISO-abbreviation] Zh. Mikrobiol. Epidemiol. Immunobiol.
  • [Language] rus
  • [Publication-type] English Abstract; Evaluation Studies; Journal Article
  • [Publication-country] Russia (Federation)
  • [Chemical-registry-number] 0 / DNA Primers; 0 / DNA, Viral; 0 / RNA, Viral
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88. Wang T, Zhang J: Synthesis of 2-acylfurans from 3-(1-alkynyl)-2-alken-1-ones via the oxidation of gold-carbene intermediates by H2O2. Dalton Trans; 2010 May 14;39(18):4270-3
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  • An efficient approach to 2,4,5-trisubstituted 2-acylfurans is described: treating 3-(1-alkynyl)-2-alken-1-ones with AuCl3 in DCM at rt in the presence of H2O2 afforded good yields of 2-acylfurans.

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  • (PMID = 20379602.001).
  • [ISSN] 1477-9234
  • [Journal-full-title] Dalton transactions (Cambridge, England : 2003)
  • [ISO-abbreviation] Dalton Trans
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Alkenes; 0 / Furans; 2465-56-7 / carbene; 7440-57-5 / Gold; BBX060AN9V / Hydrogen Peroxide; OP0UW79H66 / Methane; UC0XV6A8N9 / furan
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89. Kocher M, Frommolt P, Borberg SK, Rühl U, Steingräber M, Niewald M, Staar S, Stuschke M, Becker G, Fischedick AR, Herfarth K, Grauthoff H, Müller RP: Randomized study of postoperative radiotherapy and simultaneous temozolomide without adjuvant chemotherapy for glioblastoma. Strahlenther Onkol; 2008 Nov;184(11):572-9
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  • PATIENTS AND METHODS: From February 2002 to July 2004, n = 65 patients from 11 German centers with macroscopic complete tumor resection were randomized to receive either postoperative radiotherapy alone (RT, n = 35) or postoperative radiotherapy with simultaneous temozolomide (RT + TMZ, n = 30).
  • Patients were stratified according to age (< or =/>50 years) and WHO performance score (0-1 vs. 2).
  • RT consisted of 60 Gy in 30 fractions.
  • In the RT + TMZ arm, oral TMZ was administered daily at a dose of 75 mg/m(2) including weekends (40-42 doses).
  • Adjuvant treatment was not given, but in both arms, patients with recurrent tumors and in good condition (WHO 0-2) were scheduled for salvage chemotherapy with TMZ.
  • In total, 62/65 patients were evaluable.
  • Stratification variables were well balanced (< or = 50 years 26% vs. 20%, WHO 0-1 91% vs. 100%).
  • In the RT (RT + TMZ) arm, 76% (62%) of the progressing patients received salvage chemotherapy with TMZ, 36% (50%) had a second resection.
  • There was a time-constant trend for increased general quality of life (EORTC questionnaire QLQ C30) and brain-specific quality of life (EORTC questionnaire B20) in the combined arm.
  • Lymphopenia G3-4 was more frequent (33 vs. 6%) in the RT + TMZ arm.
  • [MeSH-major] Antineoplastic Agents, Alkylating / therapeutic use. Brain Neoplasms / drug therapy. Brain Neoplasms / radiotherapy. Dacarbazine / analogs & derivatives. Glioblastoma / drug therapy. Glioblastoma / radiotherapy

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  • (PMID = 19016015.001).
  • [ISSN] 0179-7158
  • [Journal-full-title] Strahlentherapie und Onkologie : Organ der Deutschen Röntgengesellschaft ... [et al]
  • [ISO-abbreviation] Strahlenther Onkol
  • [Language] eng
  • [Publication-type] Journal Article; Randomized Controlled Trial
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antineoplastic Agents, Alkylating; 7GR28W0FJI / Dacarbazine; 85622-93-1 / temozolomide
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90. Li X, Chai T, Wang Z, Song C, Cao H, Liu J, Zhang X, Wang W, Yao M, Miao Z: Occurrence and transmission of Newcastle disease virus aerosol originating from infected chickens under experimental conditions. Vet Microbiol; 2009 May 12;136(3-4):226-32
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  • In order to better understand airborne transmission of Newcastle disease, a model system was established and two trials were conducted.
  • The antibody response to infection was assessed by the hemagglutination inhibition (HI) test and viral shedding was detected by RT-PCR and Dot-ELISA.
  • NDV aerosol was initially detectable by RT-PCR and cell culture at day 2 or 3 post-inoculation (dpi).
  • [MeSH-minor] Aerosols. Animals. Antibodies, Viral / blood. Chick Embryo. Enzyme-Linked Immunosorbent Assay / veterinary. Hemagglutination Inhibition Tests / veterinary. RNA, Viral / chemistry. RNA, Viral / genetics. Reverse Transcriptase Polymerase Chain Reaction / veterinary. Specific Pathogen-Free Organisms. Virus Shedding

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  • (PMID = 19091492.001).
  • [ISSN] 0378-1135
  • [Journal-full-title] Veterinary microbiology
  • [ISO-abbreviation] Vet. Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Aerosols; 0 / Antibodies, Viral; 0 / RNA, Viral
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91. Roquebert B, Marcelin AG: The involvement of HIV-1 RNAse H in resistance to nucleoside analogues. J Antimicrob Chemother; 2008 May;61(5):973-5
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  • [Title] The involvement of HIV-1 RNAse H in resistance to nucleoside analogues.
  • Some recent studies have underlined the role of the entire reverse transcriptase (RT) and in particular its carboxy-terminal domain from amino acid 427 to amino acid 560 [the ribonuclease H (RNAse H) domain] in resistance to nucleoside RT inhibitors (NRTIs).
  • In vitro, the mutations H539N and D549N decreased the frequency of RT template-switching and, thereby, increased the time for excision of incorporated NRTIs, and thus enhanced NRTI resistance.
  • It has been confirmed in vivo that mutations at position 558 were statistically associated with a number of thymidine analogue mutations in a study including 144 HIV-1 patients, suggesting that it could be an accessory mutation that could reinforce NRTI resistance.
  • This article highlights evidence that mutations in RNAse H can enhance NRTI resistance, suggesting that phenotypic and genotypic analyses of clinical samples including the entire HIV-1 RT and in particular the RNAse H domain are now required to better characterize the in vivo role of the RNAse H mutations on susceptibility and response to NRTIs in HIV-1-infected patients.
  • [MeSH-major] Anti-HIV Agents / pharmacology. Drug Resistance, Multiple, Viral / genetics. HIV-1 / drug effects. HIV-1 / enzymology. Ribonuclease H / genetics. Ribonuclease H / metabolism

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  • (PMID = 18325896.001).
  • [ISSN] 1460-2091
  • [Journal-full-title] The Journal of antimicrobial chemotherapy
  • [ISO-abbreviation] J. Antimicrob. Chemother.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Anti-HIV Agents; EC 2.7.7.49 / RNA-Directed DNA Polymerase; EC 3.1.26.4 / Ribonuclease H
  • [Number-of-references] 19
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92. Youngpairoj AS, Masciotra S, Garrido C, Zahonero N, de Mendoza C, García-Lerma JG: HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4 degrees C. J Antimicrob Chemother; 2008 Jun;61(6):1217-20
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  • [Title] HIV-1 drug resistance genotyping from dried blood spots stored for 1 year at 4 degrees C.
  • BACKGROUND: Dried blood spots (DBSs) are an attractive alternative to plasma for HIV-1 drug resistance testing in resource-limited settings.
  • METHODS: A total of 40 DBSs were prepared from residual diagnostic specimens collected from HIV subtype B-infected persons and were stored with desiccant at 4 degrees C.
  • Total nucleic acids were extracted after 1 year using a modification of the Nuclisens assay.
  • Resistance testing was performed using the ViroSeq HIV-1 assay and an in-house nested RT-PCR method validated for HIV-1 subtype B that amplifies a smaller (1 kb) pol fragment.
  • Overall, resistance genotypes generated from the DBSs and plasma were highly concordant.
  • CONCLUSIONS: We show that drug resistance genotyping from DBSs stored at 4 degrees C with desiccant is highly efficient but requires the amplification of small pol fragments and the use of an in-house nested PCR protocol with quality-controlled reagents.
  • [MeSH-major] Blood / virology. Drug Resistance / genetics. HIV Infections / virology. HIV-1 / genetics. HIV-1 / isolation & purification. Microbial Sensitivity Tests / methods. Specimen Handling / methods
  • [MeSH-minor] Desiccation. Genotype. Humans. Nucleic Acids / isolation & purification. Refrigeration. Reverse Transcriptase Polymerase Chain Reaction. Sensitivity and Specificity. Time Factors


93. Pinto FL, Svensson H, Lindblad P: Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes. BMC Biotechnol; 2007;7:73
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  • [Title] Webtag: a new web tool providing tags/anchors for RT-PCR experiments with prokaryotes.
  • RESULTS: The use of a relational database, in conjunction with a series of scripts written in PHP and Perl, allows the user to rapidly obtain tags that are:.
  • CONCLUSION: This new web tool allows scientists to easily and rapidly obtain suitable tags for RT-PCR experiments, and is available at http://www.egs.uu.se/software/webtag/.

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  • (PMID = 17961214.001).
  • [ISSN] 1472-6750
  • [Journal-full-title] BMC biotechnology
  • [ISO-abbreviation] BMC Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Oligonucleotides
  • [Other-IDs] NLM/ PMC2147000
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94. Tardy J, Albucher JF, Parienté J, Chollet F: [Intravenous rt-PA for acute ischemic stroke: 69 consecutive patients managed in an emergency stroke centre]. Rev Neurol (Paris); 2007 Mar;163(3):349-57
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  • [Title] [Intravenous rt-PA for acute ischemic stroke: 69 consecutive patients managed in an emergency stroke centre].
  • [Transliterated title] Evaluation à 4 ans de la filière "Urgences AVC" de l'Hôpital Purpan (Toulouse) et analyse de 69 patients consécutifs traités par rt-PA intra-veineux.
  • INTRODUCTION: After the 2002 European agreement on the use of rt-PA for fibrinolysis within less than 3 hours after ischemic stroke, we designed a specific patient management scheme for patients referred to our center.
  • We wanted to evaluate our daily practice and to confirm that the results obtained in the randomized clinical trials with rt-PA can be reproduced in routine practice.
  • Amongst these, 25.6 per cent were treated with rt-PA (2.6 per cent of all ischemic and hemorrhagic strokes, with an average NIHSS score of 15.8 at admission [5; 25].
  • Time from onset to treatment averaged 2 h 25 min, whilst door-to-treatment time averaged 40 minutes.
  • CONCLUSION: These results in our unit confirm the feasibility, reproducibility, efficacy and safety of the rt-PA fibrinolytic treatment for ischemic stroke of less than 3 hours.
  • A "Stroke emergency" pathway appears to be a helpful option to treat as many patients as possible with the shortest possible lead times.
  • [MeSH-minor] Cerebral Hemorrhage / etiology. Emergency Medical Services. Follow-Up Studies. Humans. Infusions, Intravenous. Magnetic Resonance Imaging. Middle Aged. Patient Selection. Time Factors. Tomography, X-Ray Computed. Treatment Outcome

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  • (PMID = 17404522.001).
  • [ISSN] 0035-3787
  • [Journal-full-title] Revue neurologique
  • [ISO-abbreviation] Rev. Neurol. (Paris)
  • [Language] fre
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] France
  • [Chemical-registry-number] 0 / Fibrinolytic Agents; EC 3.4.21.68 / Tissue Plasminogen Activator
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95. Geenen MM, Bakker PJ, Kremer LC, Kastelein JJ, van Leeuwen FE: Increased prevalence of risk factors for cardiovascular disease in long-term survivors of acute lymphoblastic leukemia and Wilms tumor treated with radiotherapy. Pediatr Blood Cancer; 2010 Oct;55(4):690-7
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  • [Title] Increased prevalence of risk factors for cardiovascular disease in long-term survivors of acute lymphoblastic leukemia and Wilms tumor treated with radiotherapy.
  • We determined the prevalence of CRFs in long-term survivors of acute lymphoblastic leukemia (ALL) and Wilms tumor.
  • PROCEDURE: Adult survivors of ALL and Wilms tumor treated with radiotherapy and chemotherapy (RT + CT) or treated with chemotherapy alone (CT) were compared with sibling controls.
  • RESULTS: Seventy-nine ALL, 62 Wilms tumor survivors, and 69 control subjects (mean ages 24.5, 25.9, and 26 years, respectively) were enrolled.
  • Mean follow-up time since cancer treatment was 20.8 years.
  • In the Wilms RT + CT group significantly more survivors had hypertension (21.6% vs. 1.4%, P < 0.001) and renal insufficiency (8.1% vs. 0%, P = 0.016) compared to controls.
  • There were also more patients with multiple CRFs in the Wilms RT + CT group (16.2% vs. 2.9% in controls, P = 0.019).
  • Almost 15% of ALL RT + CT survivors had growth hormone deficiency.
  • Hypogonadism was seen in 18.9% of survivors in the Wilms RT + CT group.
  • CONCLUSIONS: Long-term survivors of ALL and Wilms tumor have unfavorable CRFs due to previous RT not CT.
  • [MeSH-major] Cardiovascular Diseases / etiology. Kidney Neoplasms / complications. Precursor Cell Lymphoblastic Leukemia-Lymphoma / complications. Wilms Tumor / complications

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  • [Copyright] Copyright 2010 Wiley-Liss, Inc.
  • (PMID = 20589650.001).
  • [ISSN] 1545-5017
  • [Journal-full-title] Pediatric blood & cancer
  • [ISO-abbreviation] Pediatr Blood Cancer
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Lipids
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96. Goodwin A, Parker S, Ghersi D, Wilcken N: Post-operative radiotherapy for ductal carcinoma in situ of the breast. Cochrane Database Syst Rev; 2009;(4):CD000563
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  • BACKGROUND: The addition of radiotherapy (RT) following breast conserving surgery (BCS) was first shown to reduce the risk of ipsilateral recurrence in the treatment of invasive breast cancer.
  • Randomised controlled trials (RCTs) have shown that RT can reduce the risk of recurrence, but assessment of potential long-term complications from addition of RT following BSC for DCIS has not been reported for women participating in RCTs.
  • OBJECTIVES: To summarise the data from RCTs testing the addition of RT to BCS for treatment of DCIS to determine the balance between the benefits and harms.
  • SEARCH STRATEGY: We searched the Cochrane Breast Cancer Group Specialised Register (January 2008), Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2008, Issue 1), MEDLINE (February 2008), and EMBASE (February 2008).
  • Three trials compared the addition of RT to BCS.
  • One trial was a two by two factorial design comparing the use of RT and tamoxifen, each separately or together, in which participants were randomised in at least one arm.

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  • [UpdateIn] Cochrane Database Syst Rev. 2013;11:CD000563 [24259251.001]
  • [UpdateOf] Cochrane Database Syst Rev. 2009;(3):CD000563 [19588320.001]
  • (PMID = 19821272.001).
  • [ISSN] 1469-493X
  • [Journal-full-title] The Cochrane database of systematic reviews
  • [ISO-abbreviation] Cochrane Database Syst Rev
  • [Language] eng
  • [Publication-type] Journal Article; Meta-Analysis; Review
  • [Publication-country] England
  • [Number-of-references] 24
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97. Arraj A, Bohatier J, Aumeran C, Bailly JL, Laveran H, Traoré O: An epidemiological study of enteric viruses in sewage with molecular characterization by RT-PCR and sequence analysis. J Water Health; 2008 Sep;6(3):351-8
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  • [Title] An epidemiological study of enteric viruses in sewage with molecular characterization by RT-PCR and sequence analysis.
  • The reverse transcriptase-polymerase chain reaction (RT-PCR) results were confirmed by sequencing, and comparative phylogenetic analysis was performed on the isolated strains.

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  • (PMID = 19108555.001).
  • [ISSN] 1477-8920
  • [Journal-full-title] Journal of water and health
  • [ISO-abbreviation] J Water Health
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Sewage
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98. Arthur Woods H, Bonnecaze RT: Insect eggs at a transition between diffusion and reaction limitation: temperature, oxygen, and water. art.woods@mso.umt.edu. J Theor Biol; 2006 Dec 21;243(4):483-92
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  • We fit the model to previously published data on development time of eggs of a sphingid moth, Manduca sexta.
  • We test then this conclusion with independent data on development times of eggs distributed across a set of temperatures generated by a thermal gradient bar.

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  • (PMID = 16930626.001).
  • [ISSN] 0022-5193
  • [Journal-full-title] Journal of theoretical biology
  • [ISO-abbreviation] J. Theor. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 059QF0KO0R / Water
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99. Cui HL, Qiao JT: Effect of lysophosphatidic acid on differentiation of embryonic neural stem cells into neuroglial cells in rats in vitro. Sheng Li Xue Bao; 2007 Dec 25;59(6):759-64
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  • RT-PCR assay was also used for analyzing the expression of LPA receptors in NSCs.
  • Exposure to LPA led to a dose-dependent increase of oligodendrocytes with the response peaked at 1.0 mumol/L, with an increased percentage of 32.6% (P<0.01) of total cells as compared to that of 8.5% in the vehicle group. (2) LPA showed no effect on the differentiation of NSCs into astrocytes. (3) RT-PCR assay showed that LPA(1) and LPA(3) receptors were strongly expressed while LPA(2) receptor expressed weakly in NSCs.

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  • (PMID = 18157468.001).
  • [ISSN] 0371-0874
  • [Journal-full-title] Sheng li xue bao : [Acta physiologica Sinica]
  • [ISO-abbreviation] Sheng Li Xue Bao
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Lysophospholipids; 0 / Receptors, Lysophosphatidic Acid; 22002-87-5 / lysophosphatidic acid
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100. Ammerlaan AC, Ararou A, Houben MP, Baas F, Tijssen CC, Teepen JL, Wesseling P, Hulsebos TJ: Long-term survival and transmission of INI1-mutation via nonpenetrant males in a family with rhabdoid tumour predisposition syndrome. Br J Cancer; 2008 Jan 29;98(2):474-9
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  • [Title] Long-term survival and transmission of INI1-mutation via nonpenetrant males in a family with rhabdoid tumour predisposition syndrome.
  • Rhabdoid tumour predisposition syndrome (RTPS) is a rare syndrome caused by inheritance of a mutated INI1 gene for which only two multigeneration families have been reported.
  • To further characterise the genotype and phenotype of RTPS, we present a third family in which at least three cousins developed an atypical teratoid/rhabdoid tumour (AT/RT) at a young age.
  • Biallelic inactivation of INI1 in the tumours, except for the meningioma, was confirmed by absence of nuclear INI1-protein staining.
  • The myoepithelioma of one of the patients carried an identical somatic rearrangement in the NF2 gene as the AT/RT, indicating that both tumours originated from a common precursor cell.
  • In conclusion, this study demonstrates for the first time transmission of a germline INI1-mutation in a RTPS family via nonpenetrant males, long-term survival of two members of this family with an AT/RT, and involvement of INI1 in the pathogenesis of myoepithelioma.
  • [MeSH-major] Chromosomal Proteins, Non-Histone / genetics. DNA-Binding Proteins / genetics. Family. Genetic Predisposition to Disease. Germ-Line Mutation. Inheritance Patterns. Penetrance. Rhabdoid Tumor / genetics. Transcription Factors / genetics
  • [MeSH-minor] Adolescent. Adult. Base Sequence. Child, Preschool. Chromosomes, Human, Pair 22. DNA Mutational Analysis. Female. Humans. Infant. Male. Microsatellite Repeats / genetics. Pedigree. Sex Characteristics. Survival Analysis. Syndrome. Time Factors

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  • (PMID = 18087273.001).
  • [ISSN] 0007-0920
  • [Journal-full-title] British journal of cancer
  • [ISO-abbreviation] Br. J. Cancer
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Chromosomal Proteins, Non-Histone; 0 / DNA-Binding Proteins; 0 / SMARCB1 protein, human; 0 / Transcription Factors
  • [Other-IDs] NLM/ PMC2361463
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