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1. Yokoyama KK, Murata T, Pan J, Nakade K, Kishikawa S, Ugai H, Kimura M, Kujime Y, Hirose M, Masuzaki S, Yamasaki T, Kurihara C, Okubo M, Nakano Y, Kusa Y, Yoshikawa A, Inabe K, Ueno K, Obata Y: Genetic materials at the gene engineering division, RIKEN BioResource Center. Exp Anim; 2010;59(2):115-24
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The genetic resources include cloned DNAs, gene libraries (e.g., cDNA and genomic DNA cloned into phage, cosmid, BAC, phosmid, and YAC), vectors, hosts, recombinant viruses, and ordered library sets derived from animal cells, including human and mouse cells, microorganisms, and viruses.

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  • (PMID = 20484845.001).
  • [ISSN] 1881-7122
  • [Journal-full-title] Experimental animals
  • [ISO-abbreviation] Exp. Anim.
  • [Language] ENG
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Review
  • [Publication-country] Japan
  • [Number-of-references] 34
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2. Palti Y, Rodriguez MF, Gahr SA, Purcell MK, Rexroad CE 3rd, Wiens GD: Identification, characterization and genetic mapping of TLR1 loci in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol; 2010 May-Jun;28(5-6):918-26
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  • Two TLR1 loci were identified from a rainbow trout bacterial artificial chromosome (BAC) library using DNA sequencing and genetic linkage analyses.
  • Full length cDNA clone and direct sequencing of four BACs revealed an intact omTLR1 open reading frame (ORF) located on chromosome 14 and a second locus on chromosome 25 that contains a TLR1 pseudogene.
  • However, due to the lack of TLR6 and 10 genes in teleost genomes and up-regulation of TLR1 mRNA in response to LPS and bacterial infection in other fish species we hypothesize an important role for omTLR1 in anti-microbial immunity.
  • Therefore, the identification of a TLR2 ortholog in rainbow trout and the development of assays to measure ligand binding and downstream signaling are critical for future elucidation of omTLR1 functions.

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  • [Copyright] Published by Elsevier Ltd.
  • (PMID = 20153434.001).
  • [ISSN] 1095-9947
  • [Journal-full-title] Fish & shellfish immunology
  • [ISO-abbreviation] Fish Shellfish Immunol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Messenger; 0 / Toll-Like Receptor 1
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3. Saul SM, Brzezinski JA 4th, Altschuler RA, Shore SE, Rudolph DD, Kabara LL, Halsey KE, Hufnagel RB, Zhou J, Dolan DF, Glaser T: Math5 expression and function in the central auditory system. Mol Cell Neurosci; 2008 Jan;37(1):153-69
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  • The basic helix-loop-helix (bHLH) transcription factor Math5 (Atoh7) is required for retinal ganglion cell (RGC) and optic nerve development.
  • Math5 expression in the CN was verified by mRNA in situ hybridization, and the identity of positive neurons was confirmed morphologically using a Math5-Cre BAC transgene with an alkaline phosphatase reporter.
  • These temporal changes are consistent with a higher-level auditory processing disorder involving the CN, potentially affecting the integration of binaural sensory information.

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  • (PMID = 17977745.001).
  • [ISSN] 1044-7431
  • [Journal-full-title] Molecular and cellular neurosciences
  • [ISO-abbreviation] Mol. Cell. Neurosci.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM067840-04; United States / NIDDK NIH HHS / DK / P60 DK20572; United States / NIDCD NIH HHS / DC / R01 DC04825; United States / NEI NIH HHS / EY / T32 EY013934; United States / NIGMS NIH HHS / GM / T32 GM07544; United States / NIDCD NIH HHS / DC / P30 DC005188; United States / NIGMS NIH HHS / GM / R01 GM067840; United States / NIDDK NIH HHS / DK / P60 DK020572; United States / NEI NIH HHS / EY / R01 EY14259; United States / NEI NIH HHS / EY / R01 EY014259-03; United States / NEI NIH HHS / EY / EY014259-03; United States / NIGMS NIH HHS / GM / GM067840-04; United States / NIDCD NIH HHS / DC / T32 DC00011; United States / NIGMS NIH HHS / GM / T32 GM007544; United States / NEI NIH HHS / EY / R01 EY014259; United States / NIDCD NIH HHS / DC / P30 DC05188; United States / NIDCD NIH HHS / DC / T32 DC000011; United States / NIDCD NIH HHS / DC / R01 DC004825
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; 0 / Atoh7 protein, mouse; 0 / Basic Helix-Loop-Helix Transcription Factors; 0 / Nerve Tissue Proteins; 0 / RNA, Messenger; 0 / Stilbamidines; EC 3.2.1.23 / beta-Galactosidase; G34N38R2N1 / Bromodeoxyuridine
  • [Other-IDs] NLM/ NIHMS38894; NLM/ PMC2266824
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4. Wester U, Bondeson ML, Edeby C, Annerén G: Clinical and molecular characterization of individuals with 18p deletion: a genotype-phenotype correlation. Am J Med Genet A; 2006 Jun 1;140(11):1164-71
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The aims of present study were to clinically and molecularly characterize the syndrome further in seven subjects with de novo 18p deletions and to perform genotype-phenotype correlation.
  • All seven subjects had terminal deletions and no interstitial deletion was observed with subtelomeric FISH analyses.
  • To define the extent of the 18p deletions and the parental origin of the deletion microsatellite- and FISH analyses were performed on genomic DNA and on lymphoblastoid cell lines of the study participants.
  • Totally 19 chromosomes, 18 specific polymorphic microsatellite markers, and 5 BAC clones were used.

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  • [Copyright] Copyright 2006 Wiley-Liss, Inc.
  • (PMID = 16691587.001).
  • [ISSN] 1552-4825
  • [Journal-full-title] American journal of medical genetics. Part A
  • [ISO-abbreviation] Am. J. Med. Genet. A
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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5. Bai XY, Shen H: [Quantitative analysis of thyroid transcription factor-1 mRNA expressions in primary lung cancer and its metastatic foci]. Nan Fang Yi Ke Da Xue Xue Bao; 2008 Jan;28(1):20-5
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  • [Title] [Quantitative analysis of thyroid transcription factor-1 mRNA expressions in primary lung cancer and its metastatic foci].
  • OBJECTIVE: To observe the expression of thyroid transcription factor-1 (TTF-1) mRNA in human normal adult type II alveolar epithelial cells, embryonic alveolar epithelial cells, and primary lung carcinoma and lymph nodes, thereby exploring the role of TTF-1 mRNA expression in the tumorigenesis, development and metastasis of lung carcinoma.
  • RESULTS: TTF-1 mRNA expression was significantly less intense in embryonic lung than in normal adult lung tissues (P= 0.000), and the two tissues both had significantly greater expression than lung adenocarcinoma, squamous cell carcinoma, small cell carcinoma and large cell carcinoma (P=0.000).
  • Lung adenocarcinoma and small cell carcinoma, with similar expression intensity (P= 0.068), showed stronger expression than squamous cell carcinoma and large cell carcinoma (P=0.000), and squamous cell carcinoma showed stronger expression than large cell carcinoma (P=0.018).
  • In lung adenocarcinoma, squamous cell carcinoma and large cell carcinoma, the intensity of TTF-1 mRNA expression was stronger in lymph node metastases than in the primary foci (P=0.003, P=0.000, P=0.019, respectively).
  • The lymph node metastases had more intense expression than the primary foci of small cell lung carcinoma (P=0.078).
  • The intensity of TTF-1 mRNA expression was greater in primary lung carcinomas with lymph node metastases than in those without metastases (P=0.026).
  • CONCLUSION: The amount of TTF-1 mRNA expression lowers in the order of normal adult lung, embryonic lung and lung carcinoma tissues.
  • In lung carcinomas, TTF-1 mRNA expression differs between the histological types, high in lung adenocarcinoma and small cell carcinoma and rather low in squamous cell carcinoma and large cell carcinoma.
  • Strong expression of TTF-1 mRNA often indicates high likeliness of lung carcinoma metastasis, and highlights the high metastatic potentials of lung adenocarcinoma, squamous cell carcinoma and large cell carcinoma.
  • [MeSH-major] Carcinoma, Squamous Cell / genetics. Lung Neoplasms / genetics. Nuclear Proteins / genetics. Transcription Factors / genetics
  • [MeSH-minor] Adenocarcinoma / genetics. Adenocarcinoma / pathology. Female. Humans. In Situ Hybridization. Lymphatic Metastasis. Male. RNA, Messenger / biosynthesis. RNA, Messenger / genetics. Thyroid Gland / metabolism. Tissue Array Analysis / methods

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  • (PMID = 18227018.001).
  • [ISSN] 1673-4254
  • [Journal-full-title] Nan fang yi ke da xue xue bao = Journal of Southern Medical University
  • [ISO-abbreviation] Nan Fang Yi Ke Da Xue Xue Bao
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Nuclear Proteins; 0 / RNA, Messenger; 0 / Transcription Factors; 0 / thyroid nuclear factor 1
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6. Conner JA, Goel S, Gunawan G, Cordonnier-Pratt MM, Johnson VE, Liang C, Wang H, Pratt LH, Mullet JE, DeBarry J, Yang L, Bennetzen JL, Klein PE, Ozias-Akins P: Sequence analysis of bacterial artificial chromosome clones from the apospory-specific genomic region of Pennisetum and Cenchrus. Plant Physiol; 2008 Jul;147(3):1396-411
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  • [Title] Sequence analysis of bacterial artificial chromosome clones from the apospory-specific genomic region of Pennisetum and Cenchrus.
  • Thirty-two ASGR bacterial artificial chromosomes (BACs) isolated from both species and one ASGR-recombining BAC from P. squamulatum, which together cover approximately 2.7 Mb of DNA, were used to investigate the genomic structure of this region.
  • Phrap assembly of 4,521 high-quality reads generated 1,341 contiguous sequences (contigs; 730 from the ASGR and 30 from the ASGR-recombining BAC in P. squamulatum, plus 580 from the C. ciliaris ASGR).
  • These putative coding regions were further analyzed in silico with reference to the rice (Oryza sativa) and sorghum (Sorghum bicolor) genomes using the resources at Gramene (www.gramene.org) and Phytozome (www.phytozome.net) and by hybridization against sorghum BAC filters.
  • [MeSH-minor] Arabidopsis Proteins / genetics. Chromosomes, Artificial, Bacterial. Cloning, Molecular. DNA Transposable Elements. Gene Duplication. Genome, Plant. Molecular Sequence Data. Oryza / genetics. Sequence Analysis, DNA. Sorghum / genetics. Synteny. Transcription Factors / genetics

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  • (PMID = 18508959.001).
  • [ISSN] 0032-0889
  • [Journal-full-title] Plant physiology
  • [ISO-abbreviation] Plant Physiol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ ED544199/ ED548719/ EU559277/ EU559280
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Arabidopsis Proteins; 0 / BABY BOOM protein, Arabidopsis; 0 / DNA Transposable Elements; 0 / Transcription Factors
  • [Other-IDs] NLM/ PMC2442526
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7. van Duin M, van Marion R, Watson JE, Paris PL, Lapuk A, Brown N, Oseroff VV, Albertson DG, Pinkel D, de Jong P, Nacheva EP, Dinjens W, van Dekken H, Collins C: Construction and application of a full-coverage, high-resolution, human chromosome 8q genomic microarray for comparative genomic hybridization. Cytometry A; 2005;63(1):10-9
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  • [Title] Construction and application of a full-coverage, high-resolution, human chromosome 8q genomic microarray for comparative genomic hybridization.
  • METHODS: A minimal tiling path contig of 702 8q-specific bacterial artificial chromosome (BAC) clones was generated with a novel computational tool (BAC Contig Assembler).
  • BAC clones were amplified by degenerative oligonucleotide primer (DOP) polymerase chain reaction and subsequently printed onto glass slides.
  • For validation of the array DNA samples of gastroesophageal and prostate cancer cell lines, and chronic myeloid leukemia specimens were used, which were previously characterized by multicolor fluorescence in situ hybridization and conventional CGH.
  • The 8q array was further tested with paraffin-embedded prostate cancer specimens.
  • In comparison with conventional CGH, the resolution of the detected changes was much improved, which was demonstrated by an amplicon of 0.7 Mb and a deletion of 0.6 Mb, both spanned by only six BAC clones.
  • [MeSH-minor] Adenocarcinoma / genetics. Cardia. Chromosome Aberrations. Fixatives. Formaldehyde. Humans. In Situ Hybridization, Fluorescence. Male. Nucleic Acid Hybridization. Oligonucleotide Array Sequence Analysis. Prostatic Neoplasms / genetics. Stomach Neoplasms / genetics. Tumor Cells, Cultured

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  • (PMID = 15619731.001).
  • [ISSN] 1552-4922
  • [Journal-full-title] Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • [ISO-abbreviation] Cytometry A
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / CA89520
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Fixatives; 1HG84L3525 / Formaldehyde
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8. Xie SG, Shi DW, Wen DH, Wang R, Xi DL: Control of bromate and THM precursors using ozonation combined system. Biomed Environ Sci; 2007 Jun;20(3):217-25
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  • The biological activated carbon (BAC) filter could effectively reduce CHCl3FP and CHCl2BrFP, but increase CHClBr2FP.

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  • (PMID = 17672213.001).
  • [ISSN] 0895-3988
  • [Journal-full-title] Biomedical and environmental sciences : BES
  • [ISO-abbreviation] Biomed. Environ. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Bromates; 0 / Trihalomethanes; 4R7X1O2820 / Chlorine; 66H7ZZK23N / Ozone
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9. Wislez M, Antoine M, Baudrin L, Poulot V, Neuville A, Pradere M, Longchampt E, Isaac-Sibille S, Lebitasy MP, Cadranel J: Non-mucinous and mucinous subtypes of adenocarcinoma with bronchioloalveolar carcinoma features differ by biomarker expression and in the response to gefitinib. Lung Cancer; 2010 May;68(2):185-91
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  • [Title] Non-mucinous and mucinous subtypes of adenocarcinoma with bronchioloalveolar carcinoma features differ by biomarker expression and in the response to gefitinib.
  • There is no optimal established therapy for treating advanced or recurrent adenocarcinoma with bronchioloalveolar carcinoma features (ADC-BAC), and it remains unclear whether chemotherapy achieves therapeutic results comparable to those seen in the more common non-small lung carcinoma subtypes.
  • In order to improve the decisions made during the treatment of advanced ADC-BAC, we attempted to better characterize the mucinous and non-mucinous ADC-BAC subtypes.
  • Fifty pathological samples were obtained from 62 patients included in a multicenter prospective phase II trial (IFCT0401) conducted to evaluate gefitinib as a first-line therapy for non-resectable ADC-BAC.
  • We demonstrated that demographic data, clinical characteristics and stage at presentation (extrathoracic versus lung metastasis, as well as TNM staging) did not distinguish between the two subtypes.
  • In contrast, three biological markers (PAS staining, TTF-1 expression and EGFR genomic gain combined with mutation analysis) enabled us to independently segregate all but 2 of the 50 patients into the mucinous and non-mucinous ADC-BAC subtypes.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / diagnosis. Adenocarcinoma, Mucinous / diagnosis. DNA-Binding Proteins / metabolism. Lung Neoplasms / diagnosis. Receptor, Epidermal Growth Factor / genetics
  • [MeSH-minor] Adult. Aged. Aged, 80 and over. Biomarkers, Tumor / analysis. Biomarkers, Tumor / genetics. Biomarkers, Tumor / metabolism. DNA Mutational Analysis. Disease Progression. Drug Resistance, Neoplasm. Female. Humans. Male. Middle Aged. Quinazolines / therapeutic use

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  • [Copyright] Copyright 2009 Elsevier Ireland Ltd. All rights reserved.
  • (PMID = 19581016.001).
  • [ISSN] 1872-8332
  • [Journal-full-title] Lung cancer (Amsterdam, Netherlands)
  • [ISO-abbreviation] Lung Cancer
  • [Language] eng
  • [Publication-type] Clinical Trial, Phase II; Comparative Study; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Biomarkers, Tumor; 0 / DNA-Binding Proteins; 0 / Quinazolines; 0 / TTF1 protein, human; EC 2.7.10.1 / Receptor, Epidermal Growth Factor; S65743JHBS / gefitinib
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10. Cohen MH, Cortazar P, Justice R, Pazdur R: Approval summary: pemetrexed maintenance therapy of advanced/metastatic nonsquamous, non-small cell lung cancer (NSCLC). Oncologist; 2010;15(12):1352-8
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  • [Title] Approval summary: pemetrexed maintenance therapy of advanced/metastatic nonsquamous, non-small cell lung cancer (NSCLC).
  • Food and Drug Administration approved pemetrexed injection (Alimta® Injection; Eli Lilly and Company, Indianapolis, IN) for maintenance treatment of patients with locally advanced or metastatic nonsquamous non-small cell lung cancer whose disease has not progressed after four cycles of platinum-based doublet induction chemotherapy.
  • Pemetrexed, 500 mg/m(2) i.v., was administered every 21 days until disease progression.
  • Treatments were well balanced with respect to baseline disease characteristics and stratification factors.
  • [MeSH-major] Antineoplastic Agents / therapeutic use. Carcinoma, Non-Small-Cell Lung / drug therapy. Drug Approval. Glutamates / therapeutic use. Guanine / analogs & derivatives. Lung Neoplasms / drug therapy
  • [MeSH-minor] Adenocarcinoma / drug therapy. Adenocarcinoma / pathology. Adenocarcinoma, Bronchiolo-Alveolar / drug therapy. Adenocarcinoma, Bronchiolo-Alveolar / pathology. Carcinoma, Large Cell / drug therapy. Carcinoma, Large Cell / pathology. Double-Blind Method. Female. Humans. Male. Middle Aged. Neoplasm Staging. Pemetrexed. Placebos. Thymidylate Synthase / antagonists & inhibitors. Treatment Outcome. United States. United States Food and Drug Administration


11. Cohen MH, Johnson JR, Chattopadhyay S, Tang S, Justice R, Sridhara R, Pazdur R: Approval summary: erlotinib maintenance therapy of advanced/metastatic non-small cell lung cancer (NSCLC). Oncologist; 2010;15(12):1344-51
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  • [Title] Approval summary: erlotinib maintenance therapy of advanced/metastatic non-small cell lung cancer (NSCLC).
  • Food and Drug Administration (FDA) approved erlotinib tablets (Tarceva®; OSI Pharmaceuticals, Inc., Melville, NY) for maintenance treatment of patients with stage IIIB/IV non-small cell lung cancer (NSCLC) whose disease had not progressed after four cycles of platinum-based first-line chemotherapy.
  • Following disease progression, 57% of placebo-treated patients received additional chemotherapy, compared with 47% of erlotinib-treated patients.
  • [MeSH-major] Antineoplastic Combined Chemotherapy Protocols / therapeutic use. Carcinoma, Non-Small-Cell Lung / drug therapy. Drug Approval. Lung Neoplasms / drug therapy. Protein Kinase Inhibitors / therapeutic use. Quinazolines / therapeutic use
  • [MeSH-minor] Adenocarcinoma, Bronchiolo-Alveolar / drug therapy. Adenocarcinoma, Bronchiolo-Alveolar / pathology. Aged. Carcinoma, Large Cell / drug therapy. Carcinoma, Large Cell / pathology. Carcinoma, Squamous Cell / drug therapy. Carcinoma, Squamous Cell / pathology. Double-Blind Method. Erlotinib Hydrochloride. Female. Humans. Immunoenzyme Techniques. Male. Neoplasm Staging. Receptor, Epidermal Growth Factor / antagonists & inhibitors. Receptor, Epidermal Growth Factor / metabolism. Treatment Outcome. United States. United States Food and Drug Administration


12. Boross P, Breukel C, van Loo PF, van der Kaa J, Claassens JW, Bujard H, Schönig K, Verbeek JS: Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector. Genesis; 2009 Nov;47(11):729-35
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  • [Title] Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector.
  • The generation of cell type specific inducible Cre transgenic mice is the most challenging and limiting part in the development of spatio-temporally controlled knockout mouse models.
  • Here we report the generation and characterization of a B lymphocyte-specific tamoxifen-inducible Cre transgenic mouse strain, LC-1-hCD19-CreER(T2).
  • [MeSH-major] B-Lymphocytes / metabolism. Chromosomes, Artificial, Bacterial. Gene Expression Regulation / drug effects. Integrases / genetics. Tamoxifen / pharmacology. Transgenes

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  • [Copyright] (c) 2009 Wiley-Liss, Inc.
  • (PMID = 19621440.001).
  • [ISSN] 1526-968X
  • [Journal-full-title] Genesis (New York, N.Y. : 2000)
  • [ISO-abbreviation] Genesis
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, CD19; 0 / DNA Primers; 094ZI81Y45 / Tamoxifen; EC 2.7.7.- / Cre recombinase; EC 2.7.7.- / Integrases
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13. Ichinokawa M, Hida Y, Kaga K, Kawada M, Niizeki H, Kondo S: A case of primary pulmonary hypertension with pulmonary tumor. Ann Thorac Cardiovasc Surg; 2010 Aug;16(4):270-2
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  • Partial lung resection was planned for diagnosis.
  • Although prostacyclin was increased to 8 ng/kg/min, she did not tolerate the decubitus position and one-lung ventilation, and her pulmonary arterial pressure rose to 110/45 mmHg.
  • While she underwent partial resection under two-lung ventilation in the decubitus position, bleeding occurred from the suture line closed by a linear stapler and was controlled by additional sutures.
  • The pathological examination revealed a bronchioloalveolar carcinoma.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / surgery. Hypertension, Pulmonary / complications. Lung Neoplasms / surgery


14. Findley SD, Cannon S, Varala K, Du J, Ma J, Hudson ME, Birchler JA, Stacey G: A fluorescence in situ hybridization system for karyotyping soybean. Genetics; 2010 Jul;185(3):727-44
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  • The development of a universal soybean (Glycine max [L.
  • We used genetically anchored BAC clones both to identify individual chromosomes in metaphase spreads and to complete a FISH-based karyotyping cocktail that permitted simultaneous identification of all 20 chromosome pairs.
  • These studies led to the identification and characterization of a reciprocal chromosome translocation between chromosomes 11 and 13 in two accessions of wild soybean.
  • [MeSH-minor] Base Sequence. Chromosomes, Artificial, Bacterial. DNA, Plant. Molecular Sequence Data. Nucleic Acid Hybridization

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  • (PMID = 20421607.001).
  • [ISSN] 1943-2631
  • [Journal-full-title] Genetics
  • [ISO-abbreviation] Genetics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Plant
  • [Other-IDs] NLM/ PMC2907198
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15. Buchanan W, Roddick F, Porter N: Removal of VUV pre-treated natural organic matter by biologically activated carbon columns. Water Res; 2008 Jul;42(13):3335-42
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  • The system uses sufficient VUV radiation (16J cm(-2)) to significantly enhance the production of biologically degradable moieties prior to treatment with biologically activated carbon (BAC).
  • The VUV-BAC process decreased the overall dissolved organic carbon (DOC) concentration of a natural water sample by 54% and 44% for the virgin carbon and previously used BAC, respectively.
  • Furthermore, VUV-BAC treatment decreased the trihalomethane (THM) formation potential (THMFP) by 60-70% and the haloacetic acid (HAA) formation potential (HAAFP) by 74%.
  • The BAC systems effectively removed the hydrogen peroxide residual produced by VUV irradiation.
  • Although nitrite formation can result from VUV treatment of natural organic matter (NOM), none was detected before or after BAC treatment.

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  • (PMID = 18502470.001).
  • [ISSN] 0043-1354
  • [Journal-full-title] Water research
  • [ISO-abbreviation] Water Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Biological Products; 0 / Nitrates; 0 / Organic Chemicals; 7440-44-0 / Carbon; BBX060AN9V / Hydrogen Peroxide
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16. Ou SH, Zell JA: Validation study of the proposed IASLC staging revisions of the T4 and M non-small cell lung cancer descriptors using data from 23,583 patients in the California Cancer Registry. J Thorac Oncol; 2008 Mar;3(3):216-27
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  • [Title] Validation study of the proposed IASLC staging revisions of the T4 and M non-small cell lung cancer descriptors using data from 23,583 patients in the California Cancer Registry.
  • BACKGROUND: We performed a validation study of the proposed International Association for the Study of Lung Cancer (IASLC) tumor, node, metastasis (TNM) and stage grouping revisions on advanced nonbronchioloalveolar carcinoma (non-BAC) non-small cell lung cancer (NSCLC).
  • METHODS: Twenty-three thousand five hundred eighty-three patients from the California Cancer Registry between 1999 and 2003 with histologically confirmed non-BAC NSCLC and complete TNM staging were identified and reclassified according to the IASLC proposed TNM revisions and new stage groupings.
  • Surveillance, Epidemiology, and End Results extent of disease codes were used to identify various T4 and M descriptors.
  • T4 due to additional nodules had significant survival advantage over other T4 and M descriptors among non-BAC NSCLC and individual histology and warrants down-staging to T3.
  • CONCLUSIONS: IASLC has greatly improved the T4 and M descriptors allowing better prognostication of advanced non-BAC NSCLC.
  • [MeSH-major] Carcinoma, Non-Small-Cell Lung / secondary. Heart Neoplasms / secondary. Lung Neoplasms / pathology. Neoplasm Staging / methods. Pleural Neoplasms / secondary. Registries / statistics & numerical data

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  • (PMID = 18317063.001).
  • [ISSN] 1556-1380
  • [Journal-full-title] Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
  • [ISO-abbreviation] J Thorac Oncol
  • [Language] eng
  • [Grant] United States / NCCDPHP CDC HHS / DP / 1U58DP00807-01; United States / NCI NIH HHS / PC / N01-PC-35136; United States / NCI NIH HHS / PC / N01-PC-35139; United States / NCI NIH HHS / PC / N01-PC-54404
  • [Publication-type] Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Validation Studies
  • [Publication-country] United States
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17. Devillard E, McIntosh FM, Newbold CJ, Wallace RJ: Rumen ciliate protozoa contain high concentrations of conjugated linoleic acids and vaccenic acid, yet do not hydrogenate linoleic acid or desaturate stearic acid. Br J Nutr; 2006 Oct;96(4):697-704
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  • In incubations with ruminal microbial fractions (bacterial fraction (BAC), protozoal fraction (PRO)), LA metabolism was very similar in strained ruminal fluid (SRF) and in the BAC, while the PRO had LA-metabolising activity an order of magnitude lower.
  • The absence of an alternative route of VA/CLA formation via desaturation of stearate was confirmed by incubations of SRF, BAC or PRO with [14C]stearate.

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  • (PMID = 17010229.001).
  • [ISSN] 0007-1145
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Linoleic Acids, Conjugated; 0 / Oleic Acids; 0 / Stearic Acids; 143-25-9 / 11-octadecenoic acid; 4ELV7Z65AP / stearic acid; 9KJL21T0QJ / Linoleic Acid
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18. Yue W, Miao Y, Li X, Wu X, Zhao A, Nakagaki M: Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system. Appl Microbiol Biotechnol; 2006 Nov;73(1):181-6
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  • [Title] Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system.
  • In this paper, we used the total fat body RNA of silkworm, Bombyx mori L. to clone and sequence a 648-bp Mn-SOD cDNA fragment through RT-PCR.
  • Furthermore, a newly established Bac-to-Bac/BmNPV Baculovirus expression system was used to overexpress the recombinant Mn-SOD enzyme in silkworm larvae.

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  • (PMID = 16804693.001).
  • [ISSN] 0175-7598
  • [Journal-full-title] Applied microbiology and biotechnology
  • [ISO-abbreviation] Appl. Microbiol. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Complementary; 63231-63-0 / RNA; EC 1.15.1.1 / Superoxide Dismutase
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19. Výborný P, Sejcková L: [Antiglaucoma drugs--content of preservatives and the patient's compliance]. Cesk Slov Oftalmol; 2006 Jul;62(4):270-2, 274
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  • The authors compare the benzalconium chloride (BAC) concentrations in individual drug used for glaucoma treating.
  • The daily dose of BAC was calculated according to the drop's size and BAC concentration.
  • The daily dose of BAC differs depending on the selected therapy markedly--doses in the range from 0 to 10 micrograms were found.
  • The BAC concentration is one of important factors affecting the patient's compliance in the treatment of the primary open angle glaucoma.

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  • (PMID = 16895060.001).
  • [ISSN] 1211-9059
  • [Journal-full-title] Ceská a slovenská oftalmologie : casopis Ceské oftalmologické spolecnosti a Slovenské oftalmologické spolecnosti
  • [ISO-abbreviation] Cesk Slov Oftalmol
  • [Language] cze
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Czech Republic
  • [Chemical-registry-number] 0 / Benzalkonium Compounds; 0 / Ophthalmic Solutions; 0 / Preservatives, Pharmaceutical
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20. Dar M, Giesler T, Richardson R, Cai C, Cooper M, Lavasani S, Kille P, Voet T, Vermeesch J: Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions. BMC Biotechnol; 2008;8:86
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  • [Title] Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions.
  • In high resolution array CGH experiments, HyPer5 is demonstrated to detect chromosomal aberrations at loci 2p21-16.3 and 15q26.3-26.2 from three patient sample using bacterial artificial chromosome (BAC) arrays.

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  • (PMID = 19014508.001).
  • [ISSN] 1472-6750
  • [Journal-full-title] BMC biotechnology
  • [ISO-abbreviation] BMC Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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21. Gu X, Greiner ER, Mishra R, Kodali R, Osmand A, Finkbeiner S, Steffan JS, Thompson LM, Wetzel R, Yang XW: Serines 13 and 16 are critical determinants of full-length human mutant huntingtin induced disease pathogenesis in HD mice. Neuron; 2009 Dec 24;64(6):828-40
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  • [Title] Serines 13 and 16 are critical determinants of full-length human mutant huntingtin induced disease pathogenesis in HD mice.
  • The N-terminal 17 amino acids of huntingtin (NT17) can be phosphorylated on serines 13 and 16; however, the significance of these modifications in Huntington's disease pathogenesis remains unknown.
  • In this study, we developed BAC transgenic mice expressing full-length mutant huntingtin (fl-mhtt) with serines 13 and 16 mutated to either aspartate (phosphomimetic or SD) or alanine (phosphoresistant or SA).
  • However, fl-mhtt-induced disease pathogenesis, including motor and psychiatric-like behavioral deficits, mhtt aggregation, and selective neurodegeneration are abolished in SD but preserved in SA mice.
  • Together, our findings demonstrate that serines 13 and 16 are critical determinants of fl-mhtt-induced disease pathogenesis in vivo, supporting the targeting of huntingtin NT17 domain and its modifications in HD therapy.


22. Yamamoto R, Uenishi H, Yasue H, Takagaki Y, Sato E: The genomic structure and a novel alternatively spliced form of porcine pTalpha chain. Mol Immunol; 2007 Jan;44(4):591-7
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  • A complete genomic nucleotide sequence for porcine pTalpha gene was obtained from a BAC clone, which revealed a novel exon 2 missing in human and murine counterparts.
  • Using thymocyte RNA and RT-PCR, three types of porcine pTalpha-chain cDNA sequences, pTalpha1, pTalpha2 and pTalpha3, were obtained.
  • Using RT-PCR, pTalpha3 appeared expressed predominantly in the thymocyte RNA.
  • [MeSH-major] Alternative Splicing. Genome. Membrane Glycoproteins / genetics. Receptors, Antigen, T-Cell, alpha-beta / genetics. Swine / genetics

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  • (PMID = 16712934.001).
  • [ISSN] 0161-5890
  • [Journal-full-title] Molecular immunology
  • [ISO-abbreviation] Mol. Immunol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Membrane Glycoproteins; 0 / Receptors, Antigen, T-Cell, alpha-beta; 0 / pre-T cell receptor alpha
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23. Qiao Y, Liu X, Harvard C, Nolin SL, Brown WT, Koochek M, Holden JJ, Lewis ME, Rajcan-Separovic E: Large-scale copy number variants (CNVs): distribution in normal subjects and FISH/real-time qPCR analysis. BMC Genomics; 2007;8:167
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  • In order to understand their potential significance and to facilitate interpretation of array-CGH findings in constitutional disorders and cancers, we studied 27 normal individuals (9 Caucasian; 9 African American; 9 Hispanic) using commercially available 1 Mb resolution BAC array (Spectral Genomics).
  • A selection of CNVs was further analyzed by FISH and real-time quantitative PCR (RT-qPCR).
  • FISH analysis of 6 CNVs (4 previously reported and 2 novel CNVs) in normal subjects resulted in the confirmation of copy number changes for 1 of 2 novel CNVs and 2 of 4 known CNvs. Three CNVs tested by FISH were further validated by RT-qPCR and comparable data were obtained.
  • This included the lack of copy number change by both RT-qPCR and FISH for clone RP11-100C24, one of the most common known copy number variants, as well as confirmation of deletions for clones RP11-89M16 and RP5-1011O17.
  • Further study of a small selection of CNVs indicated concordant and discordant array vs. FISH/RT-qPCR results.

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  • (PMID = 17565693.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Other-IDs] NLM/ PMC1920519
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24. Kerstens HH, Kollers S, Kommadath A, Del Rosario M, Dibbits B, Kinders SM, Crooijmans RP, Groenen MA: Mining for single nucleotide polymorphisms in pig genome sequence data. BMC Genomics; 2009;10:4
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  • Available BAC and BAC-end sequences and their naming and mapping information, all obtained from SangerInstitute FTP site, served as a rough assembly of a reference genome.
  • [MeSH-minor] Animals. Chromosomes, Artificial, Bacterial. Cluster Analysis. Genetic Markers. Sequence Alignment. Sequence Analysis, DNA / methods

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  • (PMID = 19126189.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Genetic Markers
  • [Other-IDs] NLM/ PMC2637895
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25. Sung YM, Lee KS, Kim BT, Han J, Lee EJ: Lobar mucinous bronchioloalveolar carcinoma of the lung showing negative FDG uptake on integrated PET/CT. Eur Radiol; 2005 Oct;15(10):2075-8
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  • [Title] Lobar mucinous bronchioloalveolar carcinoma of the lung showing negative FDG uptake on integrated PET/CT.
  • We present a case of bronchioloalveolar carcinoma manifesting as air-space consolidation with negative FDG uptake on integrated PET/CT in a 42-year-old woman.
  • To know this pattern of uptake especially in the case of mucin-predominant lobar bronchioloalveolar carcinoma may help avoid misdiagnosis of this particular tumor.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / diagnosis. Adenocarcinoma, Mucinous / diagnosis. Fluorodeoxyglucose F18. Lung Neoplasms / diagnosis. Positron-Emission Tomography. Tomography, X-Ray Computed

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  • (PMID = 15717197.001).
  • [ISSN] 0938-7994
  • [Journal-full-title] European radiology
  • [ISO-abbreviation] Eur Radiol
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Radiopharmaceuticals; 0Z5B2CJX4D / Fluorodeoxyglucose F18
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26. Song J, Dong H, Ma C, Zhao B, Shang G: Construction and functional characterization of an integrative form lambda Red recombineering Escherichia coli strain. FEMS Microbiol Lett; 2010 Aug 1;309(2):178-83
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  • LS-GR shows high recombination efficiency for medium copy number vector and single copy number BAC vector modifications.

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  • (PMID = 20618864.001).
  • [ISSN] 1574-6968
  • [Journal-full-title] FEMS microbiology letters
  • [ISO-abbreviation] FEMS Microbiol. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Recombinases; 0 / Viral Proteins
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27. Cheung SW, Shaw CA, Scott DA, Patel A, Sahoo T, Bacino CA, Pursley A, Li J, Erickson R, Gropman AL, Miller DT, Seashore MR, Summers AM, Stankiewicz P, Chinault AC, Lupski JR, Beaudet AL, Sutton VR: Microarray-based CGH detects chromosomal mosaicism not revealed by conventional cytogenetics. Am J Med Genet A; 2007 Aug 1;143A(15):1679-86
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  • We have developed a clinically validated, targeted BAC clone array as a platform for comparative genomic hybridization (aCGH) to enable detection of a wide range of pathologic copy number changes in DNA.
  • This enhanced ability of aCGH to detect mosaicism missed by routine chromosome analysis may be due to some combination of testing multiple cell lineages and/or failure of cytogenetically abnormal T lymphocytes to respond to mitogens.

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  • [Copyright] (c) 2007 Wiley-Liss, Inc.
  • (PMID = 17607705.001).
  • [ISSN] 1552-4833
  • [Journal-full-title] American journal of medical genetics. Part A
  • [ISO-abbreviation] Am. J. Med. Genet. A
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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28. Eggermann T, Krause-Plonka I, Wollmann HA, Zerres K, Dai G, Meyer E, Bartsch O: Supernumerary marker chromosome 7 and maternal uniparental disomy 7 in a boy with growth retardation and triangular face. Clin Dysmorphol; 2006 Jan;15(1):9-12
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  • Using fluorescence in situ hybridization and BAC clones, the supernumerary marker chromosome was found to be a highly deleted chromosome 7 with breakpoints within the pericentric euchromatin (partial trisomy 7).
  • Considering that the SMC(7) contributed very little, if any, to the phenotype of this boy, we propose that UPD7 studies should be carried out in children with pre- and postnatal growth retardation of unknown cause even in the absence of a SMC.

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  • (PMID = 16317300.001).
  • [ISSN] 0962-8827
  • [Journal-full-title] Clinical dysmorphology
  • [ISO-abbreviation] Clin. Dysmorphol.
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Euchromatin
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29. West HL, Crowley JJ, Vance RB, Franklin WA, Livingston RB, Dakhil SR, Giguere JK, Rivkin SE, Kraut M, Chansky K, Gandara DR, Southwest Oncology Group: Advanced bronchioloalveolar carcinoma: a phase II trial of paclitaxel by 96-hour infusion (SWOG 9714): a Southwest Oncology Group study. Ann Oncol; 2005 Jul;16(7):1076-80
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  • [Title] Advanced bronchioloalveolar carcinoma: a phase II trial of paclitaxel by 96-hour infusion (SWOG 9714): a Southwest Oncology Group study.
  • BACKGROUND: There are no published prospective trials of chemotherapy for advanced bronchioloalveolar carcinoma (BAC), a subtype of non-small-cell lung cancer for which there is no current standard therapy.
  • This phase II study assesses the efficacy and toxicity of 96-h paclitaxel in chemotherapy-naive patients with advanced BAC.
  • PATIENTS AND METHODS: Patients with histologically confirmed stage IIIB (with pleural effusion) or stage IV BAC were eligible.
  • The objective response rate was 14% (all partial responses, 9% confirmed); 40% of patients demonstrated stable disease.
  • CONCLUSIONS: S9714 represents the first prospective multi-institutional cooperative group trial focusing on treatment outcomes in BAC.
  • S9714 serves as a historical control for BAC patients against which future therapeutic approaches can be compared.

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  • (PMID = 15860488.001).
  • [ISSN] 0923-7534
  • [Journal-full-title] Annals of oncology : official journal of the European Society for Medical Oncology
  • [ISO-abbreviation] Ann. Oncol.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / CA12644; United States / NCI NIH HHS / CA / CA14028; United States / NCI NIH HHS / CA / CA16385; United States / NCI NIH HHS / CA / CA20319; United States / NCI NIH HHS / CA / CA22433; United States / NCI NIH HHS / CA / CA32102; United States / NCI NIH HHS / CA / CA35090; United States / NCI NIH HHS / CA / CA35119; United States / NCI NIH HHS / CA / CA35192; United States / NCI NIH HHS / CA / CA35281; United States / NCI NIH HHS / CA / CA35431; United States / NCI NIH HHS / CA / CA38926; United States / NCI NIH HHS / CA / CA42777; United States / NCI NIH HHS / CA / CA45807; United States / NCI NIH HHS / CA / CA46136; United States / NCI NIH HHS / CA / CA46368; United States / NCI NIH HHS / CA / CA58348; United States / NCI NIH HHS / CA / CA58415; United States / NCI NIH HHS / CA / CA58416; United States / NCI NIH HHS / CA / CA58658; United States / NCI NIH HHS / CA / CA58882; United States / NCI NIH HHS / CA / CA63845; United States / NCI NIH HHS / CA / CA63850; United States / NCI NIH HHS / CA / CA67575; United States / NCI NIH HHS / CA / CA67663; United States / NCI NIH HHS / CA / CA68183; United States / NCI NIH HHS / CA / CA74647; United States / NCI NIH HHS / CA / CA76462
  • [Publication-type] Clinical Trial; Clinical Trial, Phase II; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antineoplastic Agents, Phytogenic; P88XT4IS4D / Paclitaxel
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30. Swiercz R, Hałatek T, Majcherek W, Grzelińiska Z, Wasowicz W: [Toxic effect of benzalkonium chloride on animals and humans]. Med Pr; 2007;58(2):139-42
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  • Benzalkonium chloride (BAC) exerts toxic effects on microorganisms.
  • Various BAC-containing preparations used by people may induce a number of adverse effects on the human body.
  • Bearing in mind that BAC is widely used in different branches of the national economy, its toxic effect may cause a health problem of significant importance to humans.
  • The authors describe BAC toxic effects exerted on humans and laboratory animals as well as relevant hazards resulting from the use of BAC-contained preparations.

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  • (PMID = 17926503.001).
  • [ISSN] 0465-5893
  • [Journal-full-title] Medycyna pracy
  • [ISO-abbreviation] Med Pr
  • [Language] pol
  • [Publication-type] English Abstract; Journal Article; Review
  • [Publication-country] Poland
  • [Chemical-registry-number] 0 / Benzalkonium Compounds; 0 / Preservatives, Pharmaceutical
  • [Number-of-references] 25
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31. Summers RJ, Shehata BM, Bleacher JC, Stockwell C, Rapkin L: Mucinous adenocarcinoma of the lung in association with congenital pulmonary airway malformation. J Pediatr Surg; 2010 Nov;45(11):2256-9
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  • [Title] Mucinous adenocarcinoma of the lung in association with congenital pulmonary airway malformation.
  • Congenital pulmonary airway malformation (CPAM) is a rare developmental abnormality of the lung that has been associated with the presence of rhabdomyosarcoma, pleuropulmonary blastoma, and most commonly bronchioalveolar carcinoma (BAC) of the lung.
  • Here, we report the case of an 8-year-old patient who developed KRAS mutation positive stage IV mucinous adenocarcinoma of the lung in association with CPAM.
  • This case reflects the previously recognized progression of CPAM to malignancy and suggests that BAC arising in CPAM may take a more aggressive course than previously recognized.
  • [MeSH-major] Adenocarcinoma, Mucinous / diagnosis. Cystic Adenomatoid Malformation of Lung, Congenital / diagnosis. Lung Neoplasms / diagnosis. Precancerous Conditions
  • [MeSH-minor] Bronchoscopy. Child. Diagnosis, Differential. Female. Humans. Pneumonectomy / methods. Tomography, X-Ray Computed

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  • [Copyright] Copyright © 2010 Elsevier Inc. All rights reserved.
  • (PMID = 21034957.001).
  • [ISSN] 1531-5037
  • [Journal-full-title] Journal of pediatric surgery
  • [ISO-abbreviation] J. Pediatr. Surg.
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] United States
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32. Dunnick WA, Collins JT, Shi J, Westfield G, Fontaine C, Hakimpour P, Papavasiliou FN: Switch recombination and somatic hypermutation are controlled by the heavy chain 3' enhancer region. J Exp Med; 2009 Nov 23;206(12):2613-23
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  • Both class switch recombination (CSR) and somatic hypermutation (SHM) require transcription and the trans-acting factor activation-induced cytidine deaminase (AID), and must be up-regulated during antigen-dependent differentiation of B lymphocytes.
  • To test the role of the heavy chain 3' enhancers in both CSR and SHM, we used a BAC transgene of the entire heavy chain constant region locus.
  • Using Cre-loxP recombination to delete a 28-kb region that contains the four known 3' heavy chain enhancers, we isolated lines of BAC transgenic mice with an intact heavy chain locus and paired lines in the same chromosomal insertion site lacking the 3' enhancers.

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  • (PMID = 19887393.001).
  • [ISSN] 1540-9538
  • [Journal-full-title] The Journal of experimental medicine
  • [ISO-abbreviation] J. Exp. Med.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / P30 CA046592; United States / NIAID NIH HHS / AI / AI076057; United States / NCI NIH HHS / CA / CA46592; United States / NCI NIH HHS / CA / R01 CA098495; United States / NIAID NIH HHS / AI / R01 AI076057; United States / NCI NIH HHS / CA / CA098495
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 3' Untranslated Regions; 0 / Immunoglobulin Heavy Chains; EC 2.7.7.- / Cre recombinase; EC 2.7.7.- / Integrases; EC 3.5.4.5 / Cytidine Deaminase
  • [Other-IDs] NLM/ PMC2806627
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33. Liu H, Jiang H, Haltli B, Kulowski K, Muszynska E, Feng X, Summers M, Young M, Graziani E, Koehn F, Carter GT, He M: Rapid cloning and heterologous expression of the meridamycin biosynthetic gene cluster using a versatile Escherichia coli-streptomyces artificial chromosome vector, pSBAC. J Nat Prod; 2009 Mar 27;72(3):389-95
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  • Herein we describe the development of a versatile Escherichia coli-Streptomyces shuttle Bacterial Artificial Chromosomal (BAC) conjugation vector, pSBAC, to facilitate the cloning, genetic manipulation, and heterologous expression of actinomycetes secondary metabolite biosynthetic gene clusters.
  • The success of heterologous expression of such a giant gene cluster demonstrates the versatility of BAC cloning technology and paves the road for future exploration of expression of the meridamycin biosynthetic pathway in various hosts, including strains that have been optimized for polyketide production.
  • [MeSH-major] Escherichia coli / genetics. Genes, Bacterial / genetics. Macrolides / chemical synthesis. Macrolides / metabolism. Polyketide Synthases / metabolism. Streptomyces / genetics
  • [MeSH-minor] Chromosomes, Artificial. Chromosomes, Artificial, Bacterial / metabolism. Cloning, Molecular. Genetic Vectors / chemical synthesis. Genetic Vectors / genetics. Molecular Structure

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  • (PMID = 19191550.001).
  • [ISSN] 1520-6025
  • [Journal-full-title] Journal of natural products
  • [ISO-abbreviation] J. Nat. Prod.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 3-normeridamycin; 0 / Macrolides; 0 / meridamycin; 79956-01-7 / Polyketide Synthases
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34. Petersohn JD, Conquergood LR, Leung M: Acute histologic effects and thermal distribution profile of disc biacuplasty using a novel water-cooled bipolar electrode system in an in vivo porcine model. Pain Med; 2008 Jan-Feb;9(1):26-32
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  • Disc biacuplasty (D-BAC) is a novel procedure that uses two water-cooled radiofrequency electrodes in a bipolar configuration to heat a large volume of the posterior annulus fibrosus.
  • METHODS: Seven porcine lumbar discs were treated with D-BAC to assess acute effects on the treated tissue in a "worst-case"in vivo model.

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  • (PMID = 18254764.001).
  • [ISSN] 1526-2375
  • [Journal-full-title] Pain medicine (Malden, Mass.)
  • [ISO-abbreviation] Pain Med
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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35. Duchesne A, Eggen A: Radiation hybrid mapping of genes and newly identified microsatellites in candidate regions for bovine arthrogryposis-palatoschisis and progressive ataxia based on comparative data from man, mouse and rat. J Anim Breed Genet; 2005 Apr;122 Suppl 1:28-35
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  • Primer pairs were designed for 15 loci around each candidate gene, in a region of about 20 megabases and were used to screen a bovine Bacterial Artificial Chromosome (BAC) library.
  • Eighteen microsatellites were found in the identified BAC clones, 11 on BTA24 and seven on BTA16.
  • [MeSH-major] Arthrogryposis / veterinary. Ataxia / veterinary. Cattle Diseases / genetics. Cattle Diseases / pathology. Genetic Predisposition to Disease / genetics
  • [MeSH-minor] Animals. Cattle. Chromosomes, Artificial, Bacterial. DNA Primers. Humans. Mice. Microsatellite Repeats / genetics. Radiation Hybrid Mapping / veterinary. Rats


36. Chen YP, Tseng CP, Liaw LL, Wang CL, Chen IC, Wu WJ, Wu MD, Yuan GF: Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus. J Agric Food Chem; 2008 Jul 23;56(14):5639-46
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  • In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA.
  • [MeSH-minor] Amino Acid Sequence. Chromosomes, Artificial, Bacterial. Molecular Sequence Data. Phylogeny. Polyketide Synthases / genetics. Polyketide Synthases / metabolism

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  • (PMID = 18578535.001).
  • [ISSN] 1520-5118
  • [Journal-full-title] Journal of agricultural and food chemistry
  • [ISO-abbreviation] J. Agric. Food Chem.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 79956-01-7 / Polyketide Synthases; 9LHU78OQFD / Lovastatin
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37. Chattoo JP, Stevens MP, Nair V: Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis. J Virol Methods; 2006 Aug;135(2):288-91
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  • [Title] Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis.
  • Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in poultry.
  • The construction of an infectious bacterial artificial chromosome (BAC) clone of the highly oncogenic RB-1B strain of MDV has been described previously.
  • Virus reconstituted from the BAC clone induced rapid-onset lymphomas in chickens very similar to the wildtype viruses.
  • In this paper, the construction of a high-density random transposon-insertion mutant library of the RB-1B BAC clone using a high throughput in vitro transposon mutagenesis technique is described.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial. Open Reading Frames

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  • (PMID = 16650486.001).
  • [ISSN] 0166-0934
  • [Journal-full-title] Journal of virological methods
  • [ISO-abbreviation] J. Virol. Methods
  • [Language] eng
  • [Grant] United Kingdom / Biotechnology and Biological Sciences Research Council / / BBS/B/10544
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA Transposable Elements
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38. Park EJ, Kim H, Kim Y, Yi J, Choi K, Park K: Inflammatory responses may be induced by a single intratracheal instillation of iron nanoparticles in mice. Toxicology; 2010 Sep 10;275(1-3):65-71
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  • Magnetite iron nanoparticles have been widely used as contrast agents and in thermal therapy for cancer.
  • When mice were treated with iron oxide nanoparticles (250 microg/kg, 500 microg/kg, and 1mg/kg) by a single intratracheal instillation, the level of intracellular reduced glutathione (GSH) was decreased in the cells of bronchoalveolar lavage (BAL) fluid.
  • The arrest of cell cycles in G1 phase was observed, but S-phase was significantly decreased.
  • Formation of microgranuloma, which is one of the indicators for chronic inflammatory response, was observed in the alveolar space.
  • In addition, distribution of B cell and CD8+ T cell in blood lymphocytes was increased at day 28.
  • [MeSH-major] Ferric Compounds / toxicity. Inflammation Mediators / toxicity. Lung / drug effects. Lung / metabolism. Nanoparticles / toxicity. Trachea / drug effects

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  • [Copyright] (c) 2010 Elsevier Ireland Ltd. All rights reserved.
  • (PMID = 20540983.001).
  • [ISSN] 1879-3185
  • [Journal-full-title] Toxicology
  • [ISO-abbreviation] Toxicology
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Ferric Compounds; 0 / Inflammation Mediators; 1K09F3G675 / ferric oxide
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39. Maver A, Medica I, Salobir B, Tercelj-Zorman M, Sabovic M, Petrovic D, Peterlin B: Peroxisome proliferator-activated receptor gamma/Pro12Ala polymorphism and peroxisome proliferator-activated receptor gamma coactivator-1 alpha/Gly482Ser polymorphism in patients with sarcoidosis. Sarcoidosis Vasc Diffuse Lung Dis; 2008 Sep;25(1):29-35
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  • BACKGROUND AND AIM OF THE WORK: Reduced expression and activity of the peroxisome proliferator-activated receptor gamma (PPARG) have been measured in cells of bronchoalveolar lavage fluid in sarcoidosis patients.

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  • (PMID = 19070258.001).
  • [ISSN] 1124-0490
  • [Journal-full-title] Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG
  • [ISO-abbreviation] Sarcoidosis Vasc Diffuse Lung Dis
  • [Language] ENG
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] Italy
  • [Chemical-registry-number] 0 / Heat-Shock Proteins; 0 / PPAR gamma; 0 / PPARGC1A protein, human; 0 / Transcription Factors; 9007-49-2 / DNA
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40. Dominguez-Ventura A, Allen MS, Cassivi SD, Nichols FC 3rd, Deschamps C, Pairolero PC: Lung cancer in octogenarians: factors affecting morbidity and mortality after pulmonary resection. Ann Thorac Surg; 2006 Oct;82(4):1175-9
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  • [Title] Lung cancer in octogenarians: factors affecting morbidity and mortality after pulmonary resection.
  • BACKGROUND: Predictors of morbidity and mortality after pulmonary resection for lung cancer in patients 80 years of age or older are unknown.
  • METHODS: The medical records of all patients 80 years of age or older who had pulmonary resection for lung cancer from January 1985 through September 2004 were reviewed.
  • The cancer was squamous cell carcinoma in 143 patients (37.7%), adenocarcinoma in 166 (43.8%), bronchoalveolar cell carcinoma in 47 (12.4%), and other in 23 (6.1%).
  • CONCLUSIONS: Pulmonary resection for lung cancer in octogenarians is feasible.
  • [MeSH-major] Adenocarcinoma / surgery. Carcinoma, Squamous Cell / surgery. Lung Neoplasms / surgery. Pneumonectomy / statistics & numerical data

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  • (PMID = 16996903.001).
  • [ISSN] 1552-6259
  • [Journal-full-title] The Annals of thoracic surgery
  • [ISO-abbreviation] Ann. Thorac. Surg.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Netherlands
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41. Hahn C, Islamian AP, Renz H, Nockher WA: Airway epithelial cells produce neurotrophins and promote the survival of eosinophils during allergic airway inflammation. J Allergy Clin Immunol; 2006 Apr;117(4):787-94
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  • BACKGROUND: Eosinophil-epithelial cell interactions make a major contribution to asthmatic airway inflammation.
  • Neurotrophin levels are increased in bronchoalveolar lavage fluid during allergic asthma.
  • OBJECTIVE: We sought to investigate the role of neurotrophins as inflammatory mediators in eosinophil-epithelial cell interactions during the allergic immune response.
  • METHODS: Neurotrophin expression in the lung was investigated by means of immunohistochemistry and ELISA in a mouse model of chronic experimental asthma.
  • Coculture experiments were performed with airway epithelial cells and bronchoalveolar lavage fluid eosinophils.
  • RESULTS: Neurotrophin levels increased continuously during chronic allergic airway inflammation, and airway epithelial cells were the major source of NGF and BDNF within the inflamed lung.
  • Lung eosinophils expressed the BDNF and NGF receptors tropomyosin-related kinase (Trk) A and TrkB, and coculture with airway epithelial cells resulted in enhanced epithelial neurotrophin production, as well as in prolonged survival of eosinophils.
  • [MeSH-minor] Animals. Asthma / etiology. Asthma / pathology. Asthma / physiopathology. Base Sequence. Brain-Derived Neurotrophic Factor / biosynthesis. Cell Survival. Cytokines / pharmacology. Epithelial Cells / drug effects. Epithelial Cells / physiology. Female. Inflammation Mediators / metabolism. Mice. Mice, Inbred BALB C. Models, Biological. RNA, Messenger / genetics. RNA, Messenger / metabolism. Receptor, trkA / biosynthesis. Receptor, trkB / biosynthesis

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  • (PMID = 16630935.001).
  • [ISSN] 0091-6749
  • [Journal-full-title] The Journal of allergy and clinical immunology
  • [ISO-abbreviation] J. Allergy Clin. Immunol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Brain-Derived Neurotrophic Factor; 0 / Cytokines; 0 / Inflammation Mediators; 0 / Nerve Growth Factors; 0 / RNA, Messenger; EC 2.7.10.1 / Receptor, trkA; EC 2.7.10.1 / Receptor, trkB
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42. Bruno AC, Baffa O, Carneiro AO: Hybrid system for magnetic and acoustic measurement. Conf Proc IEEE Eng Med Biol Soc; 2009;2009:761-4
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  • In order to improve the spatial resolution of Biosusceptometry of Alternate Current (BAC), we are suggesting the coupling of a Doppler ultrasonic transducer with the BAC system.

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  • (PMID = 19964487.001).
  • [ISSN] 1557-170X
  • [Journal-full-title] Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
  • [ISO-abbreviation] Conf Proc IEEE Eng Med Biol Soc
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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43. Liu HZ, Yang HZ, Mi S, Cui B, Hua F, Hu ZW: Toll like receptor 2 mediates bleomycin-induced acute lung injury, inflammation and fibrosis in mice. Yao Xue Xue Bao; 2010 Aug;45(8):976-86
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  • [Title] Toll like receptor 2 mediates bleomycin-induced acute lung injury, inflammation and fibrosis in mice.
  • Anti-cancer drug bleomycin (BLM) can cause acute lung injury (ALI) which often results in pulmonary fibrosis due to a failure of resolving acute inflammatory response.
  • TLR2 inhibition attenuated BLM-induced increase of inflammatory cells in bronchoalveolar lavage fluid (BALF), and reversed the immunosuppressive microenvironment by enhancing TH1 response (P<0.05) and inhibiting TH2 (P<0.001), Treg (P<0.01) and TH17 (P<0.01) responses.
  • [MeSH-major] Acute Lung Injury / metabolism. Cytokines / secretion. Inflammation / metabolism. Pulmonary Fibrosis / metabolism. Toll-Like Receptor 2 / metabolism
  • [MeSH-minor] Animals. Bleomycin / toxicity. Bronchoalveolar Lavage Fluid. Cells, Cultured. Dendritic Cells / cytology. Dendritic Cells / metabolism. Interleukin-17 / secretion. Interleukin-23 / secretion. Interleukin-6 / secretion. Lung / metabolism. MAP Kinase Signaling System. Male. Mice. Mice, Inbred C57BL. T-Lymphocytes, Regulatory / drug effects. Th1 Cells / drug effects. Th2 Cells / drug effects

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  • (PMID = 21348427.001).
  • [ISSN] 0513-4870
  • [Journal-full-title] Yao xue xue bao = Acta pharmaceutica Sinica
  • [ISO-abbreviation] Yao Xue Xue Bao
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Cytokines; 0 / Interleukin-17; 0 / Interleukin-23; 0 / Interleukin-6; 0 / Tlr2 protein, mouse; 0 / Toll-Like Receptor 2; 11056-06-7 / Bleomycin
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44. Honey D, Caylor C, Luthi R, Kerrigan S: Comparative alcohol concentrations in blood and vitreous fluid with illustrative case studies. J Anal Toxicol; 2005 Jul-Aug;29(5):365-9
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  • The vitreous alcohol concentration (VAC) exceeded the blood alcohol concentration (BAC) in 209 cases (71%).
  • In casework where the VAC > BAC, linear regression analysis indicated an R2 value of 0.958 (n = 209) and a VAC approximately 16% higher than the BAC.
  • The VAC/BAC ratio was more variable at lower BACs (< 0.1 g/100 mL).
  • Although VAC/BAC ratios were more consistent at concentrations of 0.1 g/100 mL and above, the overall ratio ranged from 1.01 to 2.20.
  • Of the 81 cases where BAC > VAC, a total of 24 cases indicated no vitreous alcohol.
  • Unlike the VAC/BAC data set which consisted of 97% femoral blood, the source of blood in the BAC > VAC data set was slightly more variable.
  • Of the 81 cases where BAC > VAC the blood source consisted of femoral (n = 68), heart (n = 8), pleural cavity (n = 2), carotid (n = 1), jugular (n = 1), and chest blood (n = 1).

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  • (PMID = 16105262.001).
  • [ISSN] 0146-4760
  • [Journal-full-title] Journal of analytical toxicology
  • [ISO-abbreviation] J Anal Toxicol
  • [Language] eng
  • [Publication-type] Case Reports; Comparative Study; Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 3K9958V90M / Ethanol
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45. Xu F, Cheng DH, Di YF, Tan K, Li LY, Lu GX, Tan YQ: [Identification of a cryptic 1p36.3 microdeletion in a patient with Prader-Willi-like syndrome features]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2010 Oct;27(5):524-9
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  • [Title] [Identification of a cryptic 1p36.3 microdeletion in a patient with Prader-Willi-like syndrome features].
  • OBJECTIVE: To determine the karyotype of a patient with Prader-Willi-like syndrome features.
  • RESULTS: No abnormality was discovered by high resolution karyotype analysis and methylation-specific PCR studies.
  • The deleted region was shown within a 4.2 Mb in the distal 1p by 3 BAC FISH probes of 1p36 combined with real-time PCR technique.
  • Family pedigree investigation showed the chromosome abnormality was de novo.

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  • (PMID = 20931530.001).
  • [ISSN] 1003-9406
  • [Journal-full-title] Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
  • [ISO-abbreviation] Zhonghua Yi Xue Yi Chuan Xue Za Zhi
  • [Language] chi
  • [Publication-type] Case Reports; English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
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46. Reddy S, Dolzhanskaya N, Krogh J, Velinov M: A novel 1.4 Mb de novo microdeletion of chromosome 1q21.3 in a child with microcephaly, dysmorphic features and mental retardation. Eur J Med Genet; 2009 Nov-Dec;52(6):443-5
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  • [Title] A novel 1.4 Mb de novo microdeletion of chromosome 1q21.3 in a child with microcephaly, dysmorphic features and mental retardation.
  • About 1 Mb deletion in the chromosomal region 1q21.3 was identified using BAC array CGH analysis.

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  • (PMID = 19772933.001).
  • [ISSN] 1878-0849
  • [Journal-full-title] European journal of medical genetics
  • [ISO-abbreviation] Eur J Med Genet
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] Netherlands
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47. Burtey S, Riera M, Ribe E, Pennekamp P, Passage E, Rance R, Dworniczak B, Fontés M: Overexpression of PKD2 in the mouse is associated with renal tubulopathy. Nephrol Dial Transplant; 2008 Apr;23(4):1157-65
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  • Polycystin-2 (PC-2), a cation channel of the Trp family, is involved in autosomal dominant polycystic kidney disease (ADPKD) type 2 (ADPKD2).
  • In the present paper, we describe a mouse model for human PC-2 overexpression, obtained by inserting a human bacterial artificial chromosome (BAC) containing the PKD2 gene.
  • [MeSH-minor] Animals. Blotting, Western. Disease Models, Animal. Extracellular Matrix Proteins / biosynthesis. Extracellular Matrix Proteins / genetics. Female. Humans. In Situ Hybridization, Fluorescence. Male. Mice. Mice, Inbred C57BL. Mice, Transgenic. Reverse Transcriptase Polymerase Chain Reaction. Severity of Illness Index

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  • (PMID = 18048422.001).
  • [ISSN] 1460-2385
  • [Journal-full-title] Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
  • [ISO-abbreviation] Nephrol. Dial. Transplant.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Extracellular Matrix Proteins; 0 / TRPP Cation Channels; 0 / polycystic kidney disease 2 protein; 63231-63-0 / RNA
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48. Nguyen RL, Tam LW, Lefebvre PA: The LF1 gene of Chlamydomonas reinhardtii encodes a novel protein required for flagellar length control. Genetics; 2005 Mar;169(3):1415-24
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  • Here we describe the positional cloning and molecular characterization of the LF1 gene using a bacterial artificial chromosome (BAC) library.
  • Rescue of the lf1 phenotype upon transformation was obtained with clones containing the complete LF1 gene as well as clones that lack the last two exons of the gene, indicating that only the amino-terminal portion of the LF1 gene product (LF1p) is required for function.
  • Although LF1 helps regulate flagellar length, the LF1p localizes almost exclusively in the cell body, with <1% of total cellular LF1p localizing to the flagella.

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  • (PMID = 15489537.001).
  • [ISSN] 0016-6731
  • [Journal-full-title] Genetics
  • [ISO-abbreviation] Genetics
  • [Language] ENG
  • [Databank-accession-numbers] GENBANK/ AY298951
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM034437; United States / NIGMS NIH HHS / GM / GM-34437
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Protozoan; 0 / Protozoan Proteins; 0 / RNA, Protozoan
  • [Other-IDs] NLM/ PMC1449559
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49. Terzi A, Falezza G, Benato C, Genestreti G, Santo A, Furia S, Feil B, Calabrò F: Survival following complete resection of multifocal T4 node-negative NSCLC: a retrospective study. Thorac Cardiovasc Surg; 2007 Feb;55(1):44-7
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  • Patients with multifocal pure bronchiolo-alveolar carcinoma were excluded.
  • [MeSH-major] Carcinoma, Non-Small-Cell Lung / mortality. Lung Neoplasms / mortality. Lymph Nodes / pathology. Pneumonectomy / methods

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  • (PMID = 17285473.001).
  • [ISSN] 0171-6425
  • [Journal-full-title] The Thoracic and cardiovascular surgeon
  • [ISO-abbreviation] Thorac Cardiovasc Surg
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Germany
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50. Na Z, Huipeng Y, Lipan L, Cuiping C, Umashankar ML, Xingmeng L, Xiaofeng W, Bing W, Weizheng C, Cenis JL: Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system. Appl Microbiol Biotechnol; 2008 Feb;78(2):221-6
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  • [Title] Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system.
  • The recombinant baculovirus harboring canine interferon gene was rapidly generated by the BmNPV/Bac-to-Bac system that was recently developed.
  • In B. mori-derived cell lines, the expression of the recombinant protein reached maximal levels around 72-96 h post-infection.
  • [MeSH-minor] Animals. Baculoviridae / genetics. Cell Line. Cell Survival. Chromatography, Affinity. Dogs. Electrophoresis, Polyacrylamide Gel. Genetic Vectors. Glycosylation. Larva / genetics. Larva / metabolism. Molecular Sequence Data. Rhabdoviridae Infections / immunology. Sequence Analysis, DNA. Vesiculovirus / immunology

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  • (PMID = 18066544.001).
  • [ISSN] 0175-7598
  • [Journal-full-title] Applied microbiology and biotechnology
  • [ISO-abbreviation] Appl. Microbiol. Biotechnol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ EF080825
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Interferon-alpha; 0 / Recombinant Proteins
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51. Kobayashi S, Fujino N, Suzuki S, Yanai M: Successful treatment of refractory bronchioloalveolar carcinoma with S-1 (oral fluoropyrimidine). Respirology; 2009 Jul;14(5):771-2
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  • [Title] Successful treatment of refractory bronchioloalveolar carcinoma with S-1 (oral fluoropyrimidine).
  • Bronchioloalveolar carcinoma (BAC) is a distinct subtype of non-small cell lung cancer for which there is no optimal therapy for non-resectable or recurrent disease.
  • This report describes a patient with BAC refractory to conventional chemotherapy but with a partial response to S-1, oral fluoropyrimidine, resulting in symptom improvement and weaning from oxygen supplementation.
  • Our observation suggests that S-1 is a novel option for the treatment of advanced BAC.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / drug therapy. Antimetabolites, Antineoplastic / therapeutic use. Carcinoma, Non-Small-Cell Lung / drug therapy. Lung Neoplasms / drug therapy. Oxonic Acid / therapeutic use. Tegafur / therapeutic use

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  • (PMID = 19659655.001).
  • [ISSN] 1440-1843
  • [Journal-full-title] Respirology (Carlton, Vic.)
  • [ISO-abbreviation] Respirology
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] Australia
  • [Chemical-registry-number] 0 / Antimetabolites, Antineoplastic; 0 / Drug Combinations; 150863-82-4 / S 1 (combination); 1548R74NSZ / Tegafur; 5VT6420TIG / Oxonic Acid
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52. Kamiya K, Hayashi Y, Douguchi J, Hashiguchi A, Yamada T, Izumi Y, Watanabe M, Kawamura M, Horinouchi H, Shimada N, Kobayashi K, Sakamoto M: Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma. Mod Pathol; 2008 Aug;21(8):992-1001
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  • [Title] Histopathological features and prognostic significance of the micropapillary pattern in lung adenocarcinoma.
  • The micropapillary pattern is characterized by small papillary tufts with no fibrovascular core lying in spaces and has been reported as an aggressive variant of carcinoma in several organs.
  • We investigated the histopathobiological properties of the micropapillary pattern with immunohistochemistry, serial sections, and electron microscopy in lung adenocarcinoma.
  • The subjects included 383 adenocarcinoma cases, of which 184 (48%) were micropapillary pattern-positive and 199 (52%) were micropapillary pattern-negative.
  • On histology, micropapillary tufts seemed to float in the alveolar space or spaces encased by connective tissues, whereas serial sections revealed that most tufts had continuity with other tufts and even with the main tumor.
  • Negative staining for laminin and loss of basement membrane as determined by electron microscopy suggest a loss of cell-matrix contact.
  • [MeSH-major] Adenocarcinoma, Papillary / pathology. Lung Neoplasms / pathology
  • [MeSH-minor] Adult. Aged. Aged, 80 and over. Basement Membrane / ultrastructure. Biomarkers, Tumor / analysis. Cadherins / analysis. Disease-Free Survival. Female. Humans. Immunoenzyme Techniques. Ki-67 Antigen / analysis. Laminin / analysis. Lymph Nodes / pathology. Male. Middle Aged. Neoplasm Staging. Survival Rate. beta Catenin / analysis

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  • (PMID = 18516041.001).
  • [ISSN] 1530-0285
  • [Journal-full-title] Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
  • [ISO-abbreviation] Mod. Pathol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Biomarkers, Tumor; 0 / CTNNB1 protein, human; 0 / Cadherins; 0 / Ki-67 Antigen; 0 / Laminin; 0 / beta Catenin
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53. Aokage K, Ishii G, Yoshida J, Hishida T, Nishimura M, Nagai K, Ochiai A: Histological progression of small intrapulmonary metastatic tumor from primary lung adenocarcinoma. Pathol Int; 2010 Dec;60(12):765-73
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  • [Title] Histological progression of small intrapulmonary metastatic tumor from primary lung adenocarcinoma.
  • Histological typing based on the World Health Organization classification (bronchioloalveolar carcinoma, acinar, papillary, and solid subtype) was used to evaluate 234 metastases from the primary lung adenocarcinomas of 139 patients.
  • The predominant subtype of metastasis 3 mm or less in diameter was bronchioloalveolar carcinoma when the primary lesion was diagnosed as predominant bronchioloalveolar carcinoma, acinar, and papillary subtype.
  • These findings suggest that implanted cancer cells display lepidic growth in the early metastatic phase and recapitulate the morphological heterogeneity of the original tumor as the metastasis enlarges.
  • [MeSH-major] Adenocarcinoma / secondary. Lung Neoplasms / pathology. Lung Neoplasms / secondary. Neoplasm Metastasis / pathology
  • [MeSH-minor] Aged. Disease Progression. Female. Humans. Male. Middle Aged. Neoplasm Staging

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  • [Copyright] © 2010 The Authors. Pathology International © 2010 Japanese Society of Pathology and Blackwell Publishing Asia Pty Ltd.
  • (PMID = 21091834.001).
  • [ISSN] 1440-1827
  • [Journal-full-title] Pathology international
  • [ISO-abbreviation] Pathol. Int.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Australia
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54. Semba S, Iwaya K, Matsubayashi J, Serizawa H, Kataba H, Hirano T, Kato H, Matsuoka T, Mukai K: Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung. Clin Cancer Res; 2006 Apr 15;12(8):2449-54
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  • [Title] Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung.
  • PURPOSE: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling a branched network of actin filaments.
  • In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous protein 2 (WAVE2).
  • In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate its prognostic value.
  • EXPERIMENTAL DESIGN: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic stage IA to IIIA classes.
  • Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic significance of the coexpression of Arp2 and WAVE2.
  • RESULTS: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
  • The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis (P = 0.0046), and significantly lower in bronchioloalveolar carcinomas (P < 0.0001).
  • The patients whose cancer cells coexpressed them had a shorter disease-free survival time (P < 0.0001) and overall survival time (P < 0.0001).
  • CONCLUSIONS: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer metastasis.
  • [MeSH-major] Actin-Related Protein 2 / biosynthesis. Adenocarcinoma / pathology. Lung Neoplasms / pathology. Wiskott-Aldrich Syndrome Protein Family / biosynthesis
  • [MeSH-minor] Aged. Disease-Free Survival. Female. Humans. Immunohistochemistry. Male. Multivariate Analysis. Neoplasm Staging. Prognosis. Survival Analysis

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  • (PMID = 16638851.001).
  • [ISSN] 1078-0432
  • [Journal-full-title] Clinical cancer research : an official journal of the American Association for Cancer Research
  • [ISO-abbreviation] Clin. Cancer Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / ACTR2 protein, human; 0 / Actin-Related Protein 2; 0 / WASF2 protein, human; 0 / Wiskott-Aldrich Syndrome Protein Family
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55. Niang A, Bonnichon A, Ba-Fall K, Dussart C, Camara P, Vaylet F, Mbaye PS, L'Her P, Sane M, Margery J: [Lung cancer in Senegal]. Med Trop (Mars); 2007 Dec;67(6):651-6
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  • [Title] [Lung cancer in Senegal].
  • [Transliterated title] Le cancer bronchique au Sénégal.
  • In Africa the incidence of lung cancer is rising rapidly.
  • The purpose of this prospective study was to analyze clinical, therapeutic, and prognostic features of lung cancer patients treated at the Principal Hospital in Dakar between 2002 and 2007.
  • The histological diagnosis was squamous cell carcinoma in 23 cases, adenocarcinoma in 14, large-cell carcinoma in 17, small-cell lung cancer in 2, and bronchiolo-alveolar cancer in 1.
  • The much higher rate of histological diagnosis than in the sub-region is due mainly to the availability of trained personnel with access to bronchial endoscopy and CT-scan needle biopsy since September 2003.
  • Prognosis of the disease is poor because management is undertaken at an advanced stage.
  • Lung cancer is a health issue in Dakar, Senegal.
  • [MeSH-major] Carcinoma / epidemiology. Carcinoma / therapy. Lung Neoplasms / epidemiology. Lung Neoplasms / therapy

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  • (PMID = 18300532.001).
  • [ISSN] 0025-682X
  • [Journal-full-title] Médecine tropicale : revue du Corps de santé colonial
  • [ISO-abbreviation] Med Trop (Mars)
  • [Language] fre
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] France
  • [Chemical-registry-number] 0 / Antineoplastic Agents
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56. Prabhu S, Smitha RS, Punnya VA: Merkel cell carcinoma of the alveolar mucosa in a young adult: a rare case report. Br J Oral Maxillofac Surg; 2010 Jan;48(1):48-50
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  • [Title] Merkel cell carcinoma of the alveolar mucosa in a young adult: a rare case report.
  • Merkel cell carcinoma (MCC) is an extremely rare and aggressive primary neuroendocrine neoplasm of the skin with a poor prognosis.
  • We report a rare case of MCC that arose in the alveolar mucosa of a young adult.
  • [MeSH-major] Carcinoma, Merkel Cell / pathology. Mouth Mucosa / pathology. Mouth Neoplasms / pathology
  • [MeSH-minor] Adult. Alveolar Process / pathology. Chromogranins / analysis. Follow-Up Studies. Humans. Keratin-3 / analysis. Lymphatic Metastasis / pathology. Male. Mouth Floor / pathology. Oral Ulcer / pathology. Radiography, Panoramic

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  • [Copyright] Copyright 2009 The British Association of Oral and Maxillofacial Surgeons. Published by Elsevier Ltd. All rights reserved.
  • (PMID = 19167791.001).
  • [ISSN] 1532-1940
  • [Journal-full-title] The British journal of oral & maxillofacial surgery
  • [ISO-abbreviation] Br J Oral Maxillofac Surg
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] Scotland
  • [Chemical-registry-number] 0 / Chromogranins; 0 / KRT3 protein, human; 0 / Keratin-3
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57. Chen L, Li M, Li Q, Yang X, An X, Chen Y: Characterization of the bovine immunoglobulin lambda light chain constant IGLC genes. Vet Immunol Immunopathol; 2008 Aug 15;124(3-4):284-94
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  • To characterize the bovine immunoglobulin lambda light chain constant region (IGLC) genes, we have isolated a bacterial artificial chromosome (BAC) clone by a PCR based approach from a bovine genomic DNA library, constructed using a genital ridge cell line derived from a male Holstein fetus.
  • The positive BAC clone, containing the bovine IGLC genes, was fully sequenced and had a 138 kb insert.
  • This conclusion was further confirmed by a series of RT-PCR amplifications, which also showed that among these four genes the IGLC3 was preferentially expressed in cattle.
  • [MeSH-minor] Amino Acid Sequence. Animals. Base Sequence. Chromosomes, Artificial, Bacterial. Cloning, Molecular. DNA / chemistry. DNA / genetics. Gene Library. Immunoglobulin Joining Region / genetics. Immunoglobulin Joining Region / immunology. Male. Molecular Sequence Data. Phylogeny. Polymerase Chain Reaction / veterinary. Sequence Alignment

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  • (PMID = 18538861.001).
  • [ISSN] 0165-2427
  • [Journal-full-title] Veterinary immunology and immunopathology
  • [ISO-abbreviation] Vet. Immunol. Immunopathol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Immunoglobulin Constant Regions; 0 / Immunoglobulin Joining Region; 0 / Immunoglobulin Light Chains; 0 / Immunoglobulin lambda-Chains; 9007-49-2 / DNA
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58. Bhosale PR, Patnana M, Viswanathan C, Szklaruk J: The inguinal canal: anatomy and imaging features of common and uncommon masses. Radiographics; 2008 May-Jun;28(3):819-35; quiz 913
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  • A variety of benign and malignant masses can be found in the inguinal canal (IC).
  • Primary neoplasms of the IC include liposarcoma, Burkitt lymphoma, testicular carcinoma, and sarcoma.
  • Metastases to the IC can occur from alveolar rhabdomyosarcoma, monophasic sarcoma, prostate cancer, Wilms tumor, carcinoid tumor, melanoma, or pancreatic cancer.
  • In patients with a known malignancy and peritoneal carcinomatosis, the diagnosis of metastases can be suggested when a mass is detected in the IC.
  • When peritoneal disease is not evident, a mass in the IC is indicative of stage IV disease and may significantly alter clinical and surgical treatment of the patient.
  • [MeSH-major] Abdominal Neoplasms / diagnosis. Inguinal Canal / pathology. Inguinal Canal / radiography. Peritoneal Diseases / diagnosis
  • [MeSH-minor] Adult. Aged. Female. Humans. Male. Middle Aged. Rare Diseases / diagnosis

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  • [Copyright] Copyright RSNA, 2008.
  • (PMID = 18480486.001).
  • [ISSN] 1527-1323
  • [Journal-full-title] Radiographics : a review publication of the Radiological Society of North America, Inc
  • [ISO-abbreviation] Radiographics
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] United States
  • [Number-of-references] 88
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59. Emond MR, Jontes JD: Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish. Dev Biol; 2008 Sep 1;321(1):175-87
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  • The pcdhalpha/CNR gene comprises a diverse array of neuronal cell-surface proteins of the cadherin superfamily, although very little is known about their role in neural development.
  • Using whole-mount immunocytochemistry and BAC-based expression of Pcdh1alpha-GFP fusion proteins, we find that Pcdh1alpha does not appear to form stable, synaptic puncta at early stages of synaptogenesis.
  • This cell death phenotype can be attenuated by the expression of a soluble Pcdh1alpha cytoplasmic domain.
  • [MeSH-minor] Animals. Cell Death. Central Nervous System / cytology. Central Nervous System / embryology. Embryo, Nonmammalian / metabolism. Protein Structure, Tertiary

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  • (PMID = 18602383.001).
  • [ISSN] 1095-564X
  • [Journal-full-title] Developmental biology
  • [ISO-abbreviation] Dev. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Cadherins
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60. Williams LE, Detter C, Barry K, Lapidus A, Summers AO: Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing. Appl Environ Microbiol; 2006 Jul;72(7):4899-906
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  • Sequencing of the large (>50 kb), low-copy-number (<5 per cell) plasmids that mediate horizontal gene transfer has been hindered by the difficulty and expense of isolating DNA from individual plasmids of this class.
  • We report here that a kit method previously devised for purification of bacterial artificial chromosomes (BACs) can be adapted for effective preparation of individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria.
  • Adaptation of this BAC method for large plasmid isolation removes one major technical hurdle to expanding our knowledge of the natural plasmid gene pool.
  • [MeSH-major] DNA, Bacterial / isolation & purification. Genetic Techniques. Genome, Bacterial / genetics. Plasmids / isolation & purification. Sequence Analysis, DNA
  • [MeSH-minor] Chromosomes, Artificial, Bacterial / genetics. Corynebacterium / genetics. Escherichia coli / genetics. Magnetics. Microspheres. Molecular Sequence Data. Staphylococcus / genetics

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  • (PMID = 16820486.001).
  • [ISSN] 0099-2240
  • [Journal-full-title] Applied and environmental microbiology
  • [ISO-abbreviation] Appl. Environ. Microbiol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ DQ364638/ DQ390454/ DQ390455/ DQ390456/ DQ390457/ DQ390458
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
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61. Ingvardsen CR, Xing Y, Frei UK, Lübberstedt T: Genetic and physical fine mapping of Scmv2, a potyvirus resistance gene in maize. Theor Appl Genet; 2010 May;120(8):1621-34
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  • We fine mapped Scmv2 to a region of 0.28 cM, covering a physical distance of 1.3426 Mb, and developed six new polymorphic SSR markers based on publicly available BAC sequences within this region.
  • Analysis of the public B73 BAC library as well as the syntenic rice region did not reveal any similarity to known resistance genes.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial. Chromosomes, Plant / genetics. DNA, Plant / metabolism. Expressed Sequence Tags. Genes, Plant. Genetic Markers. Models, Genetic. Mosaic Viruses / genetics. Oryza / genetics. Physical Chromosome Mapping. Plant Diseases / genetics. Polymorphism, Genetic

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  • (PMID = 20155410.001).
  • [ISSN] 1432-2242
  • [Journal-full-title] TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
  • [ISO-abbreviation] Theor. Appl. Genet.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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62. Pedrosa-Harand A, Kami J, Gepts P, Geffroy V, Schweizer D: Cytogenetic mapping of common bean chromosomes reveals a less compartmentalized small-genome plant species. Chromosome Res; 2009;17(3):405-17
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  • Cytogenetic maps of common bean chromosomes 3, 4 and 7 were constructed by fluorescence in-situ hybridization (FISH) of BAC and a few other genomic clones.
  • Although all clones were selected with genetically mapped markers, mostly with single-copy RFLPs, a large subset of BACs, from 13 different genomic regions, contained repetitive sequences, as concluded from the regional distribution patterns of multiple FISH signals on chromosomes: pericentromeric, subtelomeric and dispersed.
  • Pericentromeric repeats were present in all 11 chromosome pairs with different intensities, whereas subtelomeric repeats were present in several chromosome ends, but with different signal intensities depending on the BAC, suggesting that the terminal heterochromatin fraction of this species may be composed of different repeats.
  • [MeSH-minor] Chromosome Mapping. Chromosomes, Artificial, Bacterial. In Situ Hybridization, Fluorescence. Recombination, Genetic

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  • (PMID = 19330455.001).
  • [ISSN] 1573-6849
  • [Journal-full-title] Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
  • [ISO-abbreviation] Chromosome Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
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63. Kaplan S, Prato CG: Impact of BAC limit reduction on different population segments: a Poisson fixed effect analysis. Accid Anal Prev; 2007 Nov;39(6):1146-54
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  • [Title] Impact of BAC limit reduction on different population segments: a Poisson fixed effect analysis.
  • Over the past few decades, several countries enacted the reduction of the legal blood alcohol concentration (BAC) limit, often alongside the administrative license revocation or suspension, to battle drinking-and-driving behavior.
  • The present analysis focuses on the evaluation of the impact of BAC limit reduction on different population segments.
  • Model estimates demonstrate that, for alcohol-related single-vehicle crashes, (i) BAC laws are more effective in terms of reduction of number of casualties rather than number of accidents, (ii) women and elderly population exhibit higher law compliance with respect to men and to young adult and adult population, respectively, and (iii) the presence of passengers in the vehicle enhances the sense of responsibility of the driver.


64. Barnett NP, Wei J, Czachowski C: Measured alcohol content in college party mixed drinks. Psychol Addict Behav; 2009 Mar;23(1):152-6
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  • Standard drink equivalents were calculated and blood alcohol concentrations (BACs) for men and women were estimated.
  • Estimated BACs varied widely depending on drink alcohol concentration, but in most cases a heavy drinking episode for both men and women resulted in an estimated BAC at or above .08.

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  • (PMID = 19290700.001).
  • [ISSN] 0893-164X
  • [Journal-full-title] Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors
  • [ISO-abbreviation] Psychol Addict Behav
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 3K9958V90M / Ethanol
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65. Zheng CX, Zhao SX, Wang P, Yu HM, Wang CF, Han B, Su B, Xiang Y, Li XS, Li SX, Ma QY, Zhang RX, Wan HY, Song HD: Different expression of mimecan as a marker for differential diagnosis between NSCLC and SCLC. Oncol Rep; 2009 Nov;22(5):1057-61
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  • [Title] Different expression of mimecan as a marker for differential diagnosis between NSCLC and SCLC.
  • Mimecan mRNA was present in a limited number of mouse and human tissues, however, abundant mimecan mRNA was observed in the lung tissue.
  • Therefore, we hypothesize that mimecan could serve as a biomarker for differentiating various histological types of lung cancers.
  • In humans, the mimecan mRNA was found most abundant in ovary and less abundant in lung by using Northern blot analysis.
  • Moreover, the mimecan was expressed strongly in the epithelial cells of the bronchial wall and weaker in the epithelial cells of the alveolar sacs by in situ hybridization and immunohistochemical analysis.
  • Furthermore, the mimecan immunoreactivity was found in 103 (97.2%) of 106 non-small cell lung cancers (NSCLCs).
  • Nevertheless, a large majority of small cell lung cancers (SCLCs) (50/56, 89.3%) showed negative immunoreactivity to mimecan polyclonal antibody.
  • [MeSH-major] Biomarkers, Tumor / metabolism. Carcinoma, Non-Small-Cell Lung / diagnosis. Intercellular Signaling Peptides and Proteins / metabolism. Lung Neoplasms / diagnosis. Small Cell Lung Carcinoma / diagnosis
  • [MeSH-minor] Blotting, Northern. Case-Control Studies. Diagnosis, Differential. Humans. Immunoenzyme Techniques. In Situ Hybridization. Prognosis

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  • (PMID = 19787221.001).
  • [ISSN] 1021-335X
  • [Journal-full-title] Oncology reports
  • [ISO-abbreviation] Oncol. Rep.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Greece
  • [Chemical-registry-number] 0 / Biomarkers, Tumor; 0 / Intercellular Signaling Peptides and Proteins; 0 / OGN protein, human
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66. Makushkin EO, Korsunov VM, Pavlova II: [The biomass of microbial communities in different types of alluvial soils in the upper reach of the Selenga River estuary]. Izv Akad Nauk Ser Biol; 2009 Jan-Feb;(1):100-7
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  • The bacterial complex predominated at the beginning of July; actinomycete and spore-fungal complexes, at the end of August; and spore-fungal and actinomycete complexes, at the beginning of October.

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  • (PMID = 19239119.001).
  • [ISSN] 1026-3470
  • [Journal-full-title] Izvestiia Akademii nauk. Seriia biologicheskaia
  • [ISO-abbreviation] Izv. Akad. Nauk. Ser. Biol.
  • [Language] RUS
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Russia (Federation)
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67. Kowalski W, Mierzwa T, Grabiec M, Laskowski R, Walentowicz M: [Problems in diagnosing breast cancer in women younger than 25 years old]. Ginekol Pol; 2006 May;77(5):376-82
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  • [Title] [Problems in diagnosing breast cancer in women younger than 25 years old].
  • OBJECTIVES: There are several challenges in the diagnosis of breast cancer in young women.
  • Special consideration must be given to the woman's fertility and body image issues, but the difficulty in distinguishing benign from malignant lesions in this group of patients still exists.
  • The aim of our study was to show five cases of breast cancer in young women.
  • MATERIALS AND METHODS: Based on 5 cases of breast cancer in women younger than 25, several methods used in diagnosis in this group of patients were discussed.
  • The role of USG and BAC was analyzed.
  • RESULTS: Diagnosing and treatment of young patients with breast cancer should be performed in specialized oncological centers experienced in dealing with breast cancer in very young women.
  • Because of the possibility of breast cancer in women under 25 years old, each case of breast tumor should be precisely diagnosed, especially by ultrasonography and biopsy (BAC) 2.
  • Each case suspected of breast cancer in young women should be diagnosed in highly equipped and specialized medical center.
  • [MeSH-major] Breast Neoplasms / diagnosis
  • [MeSH-minor] Adult. Age Factors. Combined Modality Therapy. Diagnosis, Differential. Female. Humans. Mammography / methods

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  • (PMID = 16958227.001).
  • [ISSN] 0017-0011
  • [Journal-full-title] Ginekologia polska
  • [ISO-abbreviation] Ginekol. Pol.
  • [Language] pol
  • [Publication-type] Case Reports; English Abstract; Journal Article
  • [Publication-country] Poland
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68. Rösch C, Eilmus S, Bothe H: Approaches to assess the biodiversity of bacteria in natural habitats. Biochem Soc Trans; 2006 Feb;34(Pt 1):169-73
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  • Any attempt to characterize a bacterial community and their functional genes coding for enzymes of the nitrogen cycle is faced with its extreme biodiversity.
  • Computer-based assignment tools have now been developed utilizing terminal restriction fragments obtained from digestions with multiple restriction enzymes.
  • Such programs allow the gross characterization of bacterial life in any complex bacterial community with confidence.

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  • (PMID = 16417513.001).
  • [ISSN] 0300-5127
  • [Journal-full-title] Biochemical Society transactions
  • [ISO-abbreviation] Biochem. Soc. Trans.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Ribosomal, 16S
  • [Number-of-references] 37
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69. McCartt AT, Blackman K, Voas RB: Implementation of Washington State's zero tolerance law: patterns of arrests, dispositions, and recidivism. Traffic Inj Prev; 2007 Dec;8(4):339-45
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  • The present study examined effects of the ZT law on arrests and case dispositions among underage offenders as a function of blood alcohol concentration (BAC) and post-law patterns of recidivism.
  • RESULTS: There was a substantial increase in arrests of underage drivers beginning immediately after implementation of the ZT law, especially among drivers with low BACs.
  • Underage offenders with lower BACs became far more likely to receive alcohol-related convictions and/or license suspensions.
  • However, the percentage of underage offenders with higher BACs receiving DUI convictions declined as some of these offenders received the lesser ZT disposition.
  • After the ZT law, underage offenders with BACs of 0.10 g/dL or higher were more likely to recidivate than those with lower BACs, but appreciable proportions of drivers were re-arrested for another alcohol offense, whatever the BAC and however they were penalized.
  • However, recidivism remains an issue as more than one in four underage drivers arrested with low BACs subsequently were re-arrested.

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  • (PMID = 17994486.001).
  • [ISSN] 1538-9588
  • [Journal-full-title] Traffic injury prevention
  • [ISO-abbreviation] Traffic Inj Prev
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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70. Kim YK, Kwon JT, Choi JY, Jiang HL, Arote R, Jere D, Je YH, Cho MH, Cho CS: Suppression of tumor growth in xenograft model mice by programmed cell death 4 gene delivery using folate-PEG-baculovirus. Cancer Gene Ther; 2010 Nov;17(11):751-60
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  • [Title] Suppression of tumor growth in xenograft model mice by programmed cell death 4 gene delivery using folate-PEG-baculovirus.
  • Cancer gene therapy using tumor suppressor genes is considered to be an attractive approach for arresting cell growth and inducing apoptosis.
  • Programmed cell death 4 (Pdcd4) is a tumor suppressor gene, which prevents tumorigenesis and tumor progression.
  • Folate-PEG-baculovirus containing Pdcd4 gene (F-P-Bac-Pdcd4) was constructed by attachment of F-PEG to the baculovirus surface using chemical modification.
  • The F-P-Bac-Pdcd4 showed enhanced transduction efficiency, efficiently expressed PDCD4 protein, and induced apoptosis in human epidermal carcinoma (KB) cells as compared with an unmodified baculovirus.
  • In a tumor xenograft study, injection of F-P-Bac-Pdcd4 into tumors established from the KB cell line by subcutaneous implantation significantly suppressed tumor growth and induced apoptosis.
  • Thus, this study shows a new baculovirus-mediated tumor suppressor gene delivery system for cancer therapy.
  • [MeSH-major] Apoptosis Regulatory Proteins / metabolism. Baculoviridae / genetics. Carcinoma / therapy. Genes, Tumor Suppressor. RNA-Binding Proteins / metabolism. Transduction, Genetic
  • [MeSH-minor] Animals. Cell Line, Tumor. Folic Acid / analogs & derivatives. Folic Acid / metabolism. Gene Expression Regulation, Neoplastic. Genetic Therapy. Humans. Male. Mice. Mice, Inbred BALB C. Mice, Nude. Polyethylene Glycols. Xenograft Model Antitumor Assays

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  • (PMID = 20539318.001).
  • [ISSN] 1476-5500
  • [Journal-full-title] Cancer gene therapy
  • [ISO-abbreviation] Cancer Gene Ther.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Apoptosis Regulatory Proteins; 0 / Pdcd4 protein, mouse; 0 / RNA-Binding Proteins; 0 / poly(ethylene glycol)-folate; 30IQX730WE / Polyethylene Glycols; 935E97BOY8 / Folic Acid
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71. Juárez P, Walters ST, Daugherty M, Radi C: A randomized trial of motivational interviewing and feedback with heavy drinking college students. J Drug Educ; 2006;36(3):233-46
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  • At an eight-week follow-up, all groups reduced their consumption, peak BAC, consequences, and dependence symptoms.

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  • (PMID = 17345916.001).
  • [ISSN] 0047-2379
  • [Journal-full-title] Journal of drug education
  • [ISO-abbreviation] J Drug Educ
  • [Language] eng
  • [Grant] United States / NIAAA NIH HHS / AA / T32-AA07465
  • [Publication-type] Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural
  • [Publication-country] United States
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72. Kato Y, Kaneko M, Sata M, Fujita N, Tsuruo T, Osawa M: Enhanced expression of Aggrus (T1alpha/podoplanin), a platelet-aggregation-inducing factor in lung squamous cell carcinoma. Tumour Biol; 2005 Jul-Aug;26(4):195-200
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  • [Title] Enhanced expression of Aggrus (T1alpha/podoplanin), a platelet-aggregation-inducing factor in lung squamous cell carcinoma.
  • Aggrus (T1alpha/podoplanin, known as a specific marker for type I alveolar cells or lymphatic endothelial cells) is a transmembrane sialoglycoprotein that aggregates platelets.
  • The present study investigates Aggrus expression in human primary lung cancer tissues of different types.
  • Microarray analysis demonstrated that aggrus was significantly expressed in squamous cell carcinoma (10/15; 66.7%).
  • Immunohistochemical analysis also showed that the incidence of positive staining in sections of squamous cell carcinoma (7/8; 87.5%) was higher than that in adenocarcinoma (2/13; 15.4%).
  • Furthermore, Aggrus expression was detected in a squamous cell carcinoma cell line, NCI-H226, by real-time PCR.
  • These findings indicated that overexpression of Aggrus occurred in squamous cell carcinoma of the lung.
  • Therefore, Aggrus could be a useful diagnostic marker for squamous cell carcinoma of the lung.
  • [MeSH-major] Biomarkers, Tumor / analysis. Carcinoma, Squamous Cell / metabolism. Lung Neoplasms / metabolism. Membrane Proteins / biosynthesis
  • [MeSH-minor] Cell Line, Tumor. Humans. Immunohistochemistry. Oligonucleotide Array Sequence Analysis. Reverse Transcriptase Polymerase Chain Reaction

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  • [Copyright] Copyright (c) 2005 S. Karger AG, Basel.
  • (PMID = 16006773.001).
  • [ISSN] 1010-4283
  • [Journal-full-title] Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
  • [ISO-abbreviation] Tumour Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Biomarkers, Tumor; 0 / Membrane Proteins; 0 / T1A-2 protein, human
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73. Safár J, Simková H, Kubaláková M, Cíhalíková J, Suchánková P, Bartos J, Dolezel J: Development of chromosome-specific BAC resources for genomics of bread wheat. Cytogenet Genome Res; 2010 Jul;129(1-3):211-23
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  • [Title] Development of chromosome-specific BAC resources for genomics of bread wheat.
  • Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries.
  • Each chromosome (arm)-specific library is composed of a manageable number of clones, and so represents a practical tool in the area of wheat genomics.
  • [MeSH-major] Chromosomes, Artificial, Bacterial / genetics. Chromosomes, Plant / genetics. Triticum / genetics

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  • [Copyright] Copyright 2010 S. Karger AG, Basel.
  • (PMID = 20501977.001).
  • [ISSN] 1424-859X
  • [Journal-full-title] Cytogenetic and genome research
  • [ISO-abbreviation] Cytogenet. Genome Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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74. Ahsan B, Kobayashi D, Yamada T, Kasahara M, Sasaki S, Saito TL, Nagayasu Y, Doi K, Nakatani Y, Qu W, Jindo T, Shimada A, Naruse K, Toyoda A, Kuroki Y, Fujiyama A, Sasaki T, Shimizu A, Asakawa S, Shimizu N, Hashimoto S, Yang J, Lee Y, Matsushima K, Sugano S, Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, Nomoto H, Nogata K, Morishita T, Endo T, Shin-I T, Takeda H, Kohara Y, Morishita S: UTGB/medaka: genomic resource database for medaka biology. Nucleic Acids Res; 2008 Jan;36(Database issue):D747-52
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  • This database provides basic genomic information, such as predicted genes, expressed sequence tags (ESTs), guanine/cytosine (GC) content, repeats and comparative genomics, as well as unique data resources including (i) 2473 genetic markers and experimentally confirmed PCR primers that amplify these markers, (ii) 142,414 bacterial artificial chromosome (BAC) and 217,344 fosmid end sequences that amount to 15.0- and 11.1-fold clone coverage of the entire genome, respectively, and were used for draft genome assembly, (iii) 16,519,460 single nucleotide polymorphisms (SNPs), and 2 859 905 insertions/deletions detected between two medaka inbred strain genomes and (iv) 841 235 5'-end serial analyses of gene-expression (SAGE) tags that identified 344 266 transcription start sites on the genome.
  • [MeSH-minor] Animals. Chromosomes, Artificial, Bacterial. Gene Expression. Genetic Markers. Genetic Variation. Internet. Plasmids / genetics. Polymorphism, Single Nucleotide. Transcription Initiation Site. User-Computer Interface

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75. Tischer BK, Kaufer BB, Sommer M, Wussow F, Arvin AM, Osterrieder N: A self-excisable infectious bacterial artificial chromosome clone of varicella-zoster virus allows analysis of the essential tegument protein encoded by ORF9. J Virol; 2007 Dec;81(23):13200-8
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  • [Title] A self-excisable infectious bacterial artificial chromosome clone of varicella-zoster virus allows analysis of the essential tegument protein encoded by ORF9.
  • In order to facilitate the generation of mutant viruses of varicella-zoster virus (VZV), the agent causing varicella (chicken pox) and herpes zoster (shingles), we generated a full-length infectious bacterial artificial chromosome (BAC) clone of the P-Oka strain.
  • Subsequently, mini-F-containing recombinant virus was generated from overlapping cosmid clones, and full-length VZV DNA recovered from the recombinant virus was established in Escherichia coli as an infectious BAC.

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  • (PMID = 17913822.001).
  • [ISSN] 1098-5514
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / R21 AI061412; United States / NIAID NIH HHS / AI / 1R21AI061412
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Viral; 0 / Viral Structural Proteins
  • [Other-IDs] NLM/ PMC2169085
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76. Yang Z, Zhang Y: [The application of nuclear medicine imaging in bronchioloalveolar carcinoma.]. Zhongguo Fei Ai Za Zhi; 2009 Oct 20;12(10):1123-6
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  • [Title] [The application of nuclear medicine imaging in bronchioloalveolar carcinoma.].

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  • (PMID = 20723354.001).
  • [ISSN] 1999-6187
  • [Journal-full-title] Zhongguo fei ai za zhi = Chinese journal of lung cancer
  • [ISO-abbreviation] Zhongguo Fei Ai Za Zhi
  • [Language] chi
  • [Publication-type] Journal Article
  • [Publication-country] China
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77. Tinianow JN, Gill HS, Ogasawara A, Flores JE, Vanderbilt AN, Luis E, Vandlen R, Darwish M, Junutula JR, Williams SP, Marik J: Site-specifically 89Zr-labeled monoclonal antibodies for ImmunoPET. Nucl Med Biol; 2010 Apr;37(3):289-97
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  • METHODS: The amino group of desferrioxamine B was acylated by bromoacetyl bromide, N-hydroxysuccinimidyl iodoacetate, or N-hydroxysuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate to obtain thiol reactive reagents bromoacetyl-desferrioxamine (Df-Bac), iodoacetyl-desferrioxamine (Df-Iac) and maleimidocyclohexyl-desferrioxamine (Df-Chx-Mal), respectively.
  • Df-Bac and Df-Iac alkylated the free thiol groups of thio-trastuzumab by nucleophilic substitution forming Df-Ac-thio-trastuzumab, while the maleimide reagent Df-Chx-Mal reacted via Michael addition to provide Df-Chx-Mal-thio-trastuzumab.
  • RESULTS: The chemoselective reagents were obtained in 14% (Df-Bac), 53% (Df-Iac) and 45% (Df-Chx-Mal) yields.
  • Site-specific conjugation of Df-Chx-Mal to thio-trastuzumab was complete within 1 h at pH 7.5, while Df-Iac and Df-Bac respectively required 2 and 5 h at pH 9.
  • Each Df modified thio-trastuzumab was chelated with (89)Zr in yields exceeding 75%. (89)Zr-Df-Ac-thio-trastuzumab and (89)Zr-Df-Chx-Mal-thio-trastuzumab were stable in mouse serum and exhibited comparable PET imaging capabilities in a BT474M1 (HER2-positive) breast cancer model reaching 20-25 %ID/g of tumor uptake and a tumor to blood ratio of 6.1-7.1.

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  • [Copyright] Copyright 2010 Elsevier Inc. All rights reserved.
  • (PMID = 20346868.001).
  • [ISSN] 1872-9614
  • [Journal-full-title] Nuclear medicine and biology
  • [ISO-abbreviation] Nucl. Med. Biol.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / Radioisotopes; 0 / Radiopharmaceuticals; C6V6S92N3C / Zirconium
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78. Liu T, Mao D, Zhang S, Xu C, Xing Y: Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa). Theor Appl Genet; 2009 May;118(8):1509-17
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  • [Title] Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa).
  • Finally, it was narrowed down to a bacterial artificial chromosome clone spanning 107 kb; 17 open reading frames have been identified in the region.
  • [MeSH-major] Chromosomes, Artificial, Bacterial. Genes, Plant. Oryza / genetics. Physical Chromosome Mapping. Quantitative Trait Loci

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  • (PMID = 19266175.001).
  • [ISSN] 1432-2242
  • [Journal-full-title] TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
  • [ISO-abbreviation] Theor. Appl. Genet.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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79. Prior K, Hautefort I, Hinton JC, Richardson DJ, Rowley G: All stressed out. Salmonella pathogenesis and reactive nitrogen species. Adv Microb Physiol; 2009;56:1-28
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  • Bacterial pathogens must overcome a range of challenges during the process of infecting their host.
  • The ability of a pathogen to sense and respond appropriately to changes in host environment is vital if the pathogen is to succeed.
  • Mammalian defense strategies include the use of barriers like skin and epithelial surfaces, the production of a chemical arsenal, such as stomach acid and reactive oxygen and nitrogen species, and a highly coordinated cellular and humoral immune response.
  • Here we focus on the interplay which occurs between Salmonella and the host during the infection process, with particular emphasis on the complex bacterial response to reactive nitrogen species produced by the host.
  • We discuss recent advances in our understanding of the key mechanisms which confer bacterial resistance to nitrogen species, which in response to nitric oxide include the flavohemoglobin, HmpA, the flavorubredoxin, NorV, and the cytochrome c nitrite reductase, NrfA, whilst in response to nitrate include a repertoire of nitrate reductases.

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  • [Copyright] Copyright © 2009 Elsevier Ltd. All rights reserved.
  • (PMID = 20943123.001).
  • [ISSN] 2162-5468
  • [Journal-full-title] Advances in microbial physiology
  • [ISO-abbreviation] Adv. Microb. Physiol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Reactive Nitrogen Species; 31C4KY9ESH / Nitric Oxide; EC 1.7.- / Nitrite Reductases
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80. Sziksz E, Tibor Kozma G, Komlósi ZI, Pállinger E, Kardos M, Szebeni B, Losonczy G, Falus A, Szabó A, Tulassay T, Vannay A: Increased synthesis of vascular endothelial growth factor in allergic airway inflammation in histidine decarboxylase knockout (HDC(-/-)) mice. Exp Lung Res; 2010 Sep;36(7):420-30
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  • VEGF mRNA expression and protein level were determined in the lung.
  • Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry.
  • VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC(-/-)(ova) as well as in WT(ova)) animals compared to their controls.
  • However, there was no difference in the VEGF levels between HDC(-/-) or WT animals, either in the lung or in the BAL cells.
  • In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals.
  • [MeSH-minor] Animals. Bronchoalveolar Lavage Fluid / cytology. Female. Lung / drug effects. Lung / enzymology. Lung / pathology. Mice. Mice, Knockout. Ovalbumin

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  • (PMID = 20715981.001).
  • [ISSN] 1521-0499
  • [Journal-full-title] Experimental lung research
  • [ISO-abbreviation] Exp. Lung Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Vascular Endothelial Growth Factors; 820484N8I3 / Histamine; 9006-59-1 / Ovalbumin; EC 4.1.1.22 / Histidine Decarboxylase
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81. Chen WY, Pulukkunat DK, Cho IM, Tsai HY, Gopalan V: Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complex. Nucleic Acids Res; 2010 Dec;38(22):8316-27
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  • [Title] Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complex.
  • Biochemical studies on the bacterial holoenzyme, composed of one catalytic RNase P RNA (RPR) and one RNase P protein (RPP), have helped understand the pleiotropic roles (including substrate/Mg(2+) binding) by which a protein could facilitate RNA catalysis.
  • Exploiting our previous finding that these archaeal RPPs function as two binary RPP complexes (POP5•RPP30 and RPP21•RPP29), we prepared recombinant RPP pairs from three archaea and established interchangeability of subunits through homologous/heterologous assemblies.
  • Our finding that archaeal POP5•RPP30 reconstituted with bacterial and organellar RPRs suggests functional overlap of this binary complex with the bacterial RPP and highlights their shared recognition of a phylogenetically-conserved RPR catalytic core, whose minimal attributes we further defined through deletion mutagenesis.

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  • (PMID = 20705647.001).
  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM067807
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Archaeal Proteins; 0 / Protein Subunits; 0 / RNA Precursors; 9014-25-9 / RNA, Transfer; EC 3.1.26.5 / Ribonuclease P
  • [Other-IDs] NLM/ PMC3001054
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82. Bosnjak B, Ivetić Tkalcević V, Durić K, Belamarić D, Cuzić S, Ferencić Z, Brajsa K, Glojnarić I, Antolović R, Hrvacić B: Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma. Inflamm Res; 2009 Nov;58(11):773-81
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  • [Title] Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma.
  • OBJECTIVE: To investigate whether challenge with increasing allergen doses could differently affect allergen-induced airway hyperresponsiveness (AHR) and inflammatory cell accumulation in mouse model of asthma, providing an experimental model to investigate their relationship.
  • MATERIAL AND METHODS: AHR and accumulation of inflammatory cells in bronchoalveolar lavage fluid (BALF) and into the lungs were compared in ovalbumin-sensitized mice that were challenged intranasally with 2.5, 10, 25 or 100 microg of ovalbumin/mouse.
  • RESULTS: Both AHR and inflammatory cell accumulation were proportional to the ovalbumin dose used for challenge.
  • [MeSH-minor] Animals. Bronchoalveolar Lavage Fluid / cytology. Bronchoalveolar Lavage Fluid / immunology. Cytokines / immunology. Disease Models, Animal. Eosinophil Peroxidase / metabolism. Eosinophils / immunology. Humans. Interleukin-13 / immunology. Lung / cytology. Lung / immunology. Lung / pathology. Male. Mice. Mice, Inbred BALB C

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  • (PMID = 19440656.001).
  • [ISSN] 1420-908X
  • [Journal-full-title] Inflammation research : official journal of the European Histamine Research Society ... [et al.]
  • [ISO-abbreviation] Inflamm. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / Cytokines; 0 / Interleukin-13; 9006-59-1 / Ovalbumin; EC 1.11.1.- / Eosinophil Peroxidase
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83. Sipos R, Székely AJ, Palatinszky M, Révész S, Márialigeti K, Nikolausz M: Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis. FEMS Microbiol Ecol; 2007 May;60(2):341-50
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  • [Title] Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis.
  • In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem.
  • The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis.
  • As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR.
  • [MeSH-minor] DNA, Bacterial / chemistry. DNA, Bacterial / genetics. Ecosystem. Electrophoresis, Capillary. Environmental Microbiology. Molecular Sequence Data. Polymorphism, Restriction Fragment Length. Sequence Analysis, DNA. Temperature

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  • (PMID = 17343679.001).
  • [ISSN] 0168-6496
  • [Journal-full-title] FEMS microbiology ecology
  • [ISO-abbreviation] FEMS Microbiol. Ecol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ DQ207728/ DQ207729/ DQ207730/ DQ207731
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA Primers; 0 / DNA, Bacterial; 0 / RNA, Ribosomal, 16S
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84. Portnoï D, Sertour N, Ferquel E, Garnier M, Baranton G, Postic D: A single-run, real-time PCR for detection and identification of Borrelia burgdorferi sensu lato species, based on the hbb gene sequence. FEMS Microbiol Lett; 2006 Jun;259(1):35-40
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  • Lyme borreliosis is the most important vector-borne disease caused by spirochetes within the Borrelia burgdorferi sensu lato (B. burgdorferi sl) complex.
  • There is strong evidence that different species of this group of genetically diverse spirochetes are involved in distinct clinical manifestations of the disease.
  • In order to differentiate species within this bacterial complex, we developed a real-time-PCR protocol, which targets the hbb gene.
  • We designed a fluorescein-labeled probe specific of a region of this gene harboring a polymorphism linked to species.
  • [MeSH-major] Bacterial Proteins / genetics. Borrelia burgdorferi Group / classification. Borrelia burgdorferi Group / isolation & purification. DNA-Binding Proteins / genetics. Polymerase Chain Reaction / methods
  • [MeSH-minor] Animals. Bacterial Typing Techniques. DNA Primers. DNA, Bacterial / analysis. DNA, Bacterial / isolation & purification. Reproducibility of Results. Sensitivity and Specificity. Ticks / microbiology. Transition Temperature

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  • (PMID = 16684099.001).
  • [ISSN] 0378-1097
  • [Journal-full-title] FEMS microbiology letters
  • [ISO-abbreviation] FEMS Microbiol. Lett.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / DNA Primers; 0 / DNA, Bacterial; 0 / DNA-Binding Proteins; 0 / Hbb protein, Borrelia burgdorferi
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85. Macfarlane S, Woodmansey EJ, Macfarlane GT: Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Appl Environ Microbiol; 2005 Nov;71(11):7483-92
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  • The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen.
  • The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes.
  • Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia.
  • Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.

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  • (PMID = 16269790.001).
  • [ISSN] 0099-2240
  • [Journal-full-title] Applied and environmental microbiology
  • [ISO-abbreviation] Appl. Environ. Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Culture Media; 0 / Fluorescent Dyes; 0 / Mucins; 0 / Oligonucleotide Probes; 0 / RNA, Ribosomal, 16S
  • [Other-IDs] NLM/ PMC1287682
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86. Gearhardt AN, Corbin WR: Body mass index and alcohol consumption: family history of alcoholism as a moderator. Psychol Addict Behav; 2009 Jun;23(2):216-25
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  • BMI, family history of alcoholism, gender, and race/ethnicity were assessed as predictors of typical drinking frequency and estimated blood alcohol concentration (BAC).

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  • [Copyright] Copyright (c) 2009 APA, all rights reserved.
  • (PMID = 19586138.001).
  • [ISSN] 0893-164X
  • [Journal-full-title] Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors
  • [ISO-abbreviation] Psychol Addict Behav
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] United States
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87. Misfeldt AM, Boyle SC, Tompkins KL, Bautch VL, Labosky PA, Baldwin HS: Endocardial cells are a distinct endothelial lineage derived from Flk1+ multipotent cardiovascular progenitors. Dev Biol; 2009 Sep 01;333(1):78-89
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  • To distinguish endocardium from other vasculature, we generated an NFATc1-nuc-LacZ BAC transgenic mouse line capable of labeling this specific endothelial subpopulation at the earliest stages of cardiac development.
  • Endocardium is specified as a cardiac cell lineage, independent from other vascular populations, responding to BMP and Wnt signals that enhance cardiomyocyte differentiation.
  • [MeSH-major] Cell Lineage / physiology. Embryonic Stem Cells / physiology. Endocardium / embryology. Endothelial Cells / physiology. Multipotent Stem Cells / physiology. Myocytes, Cardiac / physiology. Vascular Endothelial Growth Factor Receptor-2 / metabolism
  • [MeSH-minor] Animals. Antigens, Differentiation / metabolism. Cell Differentiation / physiology. Cells, Cultured. Hematopoietic Stem Cells / cytology. Hematopoietic Stem Cells / physiology. Mesoderm / cytology. Mesoderm / embryology. Mice. Mice, Transgenic. Muscle, Smooth, Vascular / cytology. Muscle, Smooth, Vascular / embryology. Myocytes, Smooth Muscle / cytology. Myocytes, Smooth Muscle / physiology. NFATC Transcription Factors / genetics

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  • (PMID = 19576203.001).
  • [ISSN] 1095-564X
  • [Journal-full-title] Developmental biology
  • [ISO-abbreviation] Dev. Biol.
  • [Language] eng
  • [Grant] United States / NHLBI NIH HHS / HL / RL1 HL092551; United States / NHLBI NIH HHS / HL / RL1HL0952551-01; United States / NIGMS NIH HHS / GM / T32 GM07347
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Differentiation; 0 / NFATC Transcription Factors; 0 / Nfatc1 protein, mouse; EC 2.7.10.1 / Vascular Endothelial Growth Factor Receptor-2
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88. Shi JJ, Cai DH, Chen XJ, Sheng HZ: Cloning and characterization of the rabbit POU5F1 gene. DNA Seq; 2008 Feb;19(1):56-61
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  • The rabbit POUSF1 gene was mapped to chromosome 12q1.1 by PCR amplification of DNA from two putative POU5F1-containing BAC clones, which were previously mapped to chromosome 12q1.1.

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  • (PMID = 17852331.001).
  • [ISSN] 1042-5179
  • [Journal-full-title] DNA sequence : the journal of DNA sequencing and mapping
  • [ISO-abbreviation] DNA Seq.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ EF062856/ EF194086
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Octamer Transcription Factor-3
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89. Titus TA, Selvig DR, Qin B, Wilson C, Starks AM, Roe BA, Postlethwait JH: The Fanconi anemia gene network is conserved from zebrafish to human. Gene; 2006 Apr 26;371(2):211-23
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  • Fanconi anemia (FA) is a complex disease involving nine identified and two unidentified loci that define a network essential for maintaining genomic stability.
  • To test the hypothesis that the FA network is conserved in vertebrate genomes, we cloned and sequenced zebrafish (Danio rerio) cDNAs and/or genomic BAC clones orthologous to all nine cloned FA genes (FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, and FANCL), and identified orthologs in the genome database for the pufferfish Tetraodon nigroviridis.
  • Evolutionarily conserved regions identified functionally important domains, since many amino acid residues mutated in human disease alleles or shown to be critical in targeted mutagenesis studies are identical in zebrafish and human.
  • [MeSH-minor] Amino Acid Sequence. Animals. Chromosomes, Artificial, Bacterial. Humans. Molecular Sequence Data. Sequence Homology, Amino Acid

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  • (PMID = 16515849.001).
  • [ISSN] 0378-1119
  • [Journal-full-title] Gene
  • [ISO-abbreviation] Gene
  • [Language] eng
  • [Grant] United States / NICHD NIH HHS / HD / HD22486; United States / NCRR NIH HHS / RR / R01RR10715
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Fanconi Anemia Complementation Group D2 Protein
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90. Moore S, Shepherd J, Perham N, Cusens B: The prevalence of alcohol intoxication in the night-time economy. Alcohol Alcohol; 2007 Nov-Dec;42(6):629-34
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  • METHOD: A random sample of 893 people were interviewed and breathalysed in 24 repeated, cross-sectional surveys over the course of a year in the city centre streets of a European capital city between 11.00 pm and 3.00 am.
  • RESULTS: Median blood alcohol concentration (BAC) in men was 0.13% (min = 0%, max = 0.33%) and in women was 0.09% (min = 0%, max = 0.27%)-which were below the threshold used to indicate 'at risk BAC' (0.15%; for men t = 9.32, P < 0.001 and for women t = 17.54, P < 0.001).
  • Men provided higher BACs than women (t = 7.17, P < 0.001).
  • The relationship between age and BAC for men described an inverted 'u', peaking at 29 years, but for women the relationship was positive and linear.
  • BAC was inversely related to the ability to remember and report the evening's consumption (z = 4.76, P < 0.001).
  • Reported consumption predicted only 12% (P < 0.001) of the variance in BAC for men and 10% (P < 0.001) for women.
  • The probability for respondents to recall past consumption diminished as BAC increased suggesting self-report data are not suitable to assess consumption in heavy drinkers.

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  • (PMID = 17660522.001).
  • [ISSN] 1464-3502
  • [Journal-full-title] Alcohol and alcoholism (Oxford, Oxfordshire)
  • [ISO-abbreviation] Alcohol Alcohol.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
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91. Philipp U, Quignon P, Scott A, André C, Breen M, Leeb T: Chromosomal assignment of the canine melanophilin gene (MLPH): a candidate gene for coat color dilution in Pinschers. J Hered; 2005;96(7):774-6
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  • To investigate the genetic cause of the coat color dilution in Pinschers, we isolated BAC clones containing the canine ortholog of the known murine color dilution gene Mlph.
  • RH mapping of the canine MLPH gene was performed using an STS marker derived from BAC sequences.
  • Additionally, one MLPH BAC clone was used as probe for FISH mapping, and the canine MLPH gene was assigned to CFA25q24.

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  • (PMID = 15958794.001).
  • [ISSN] 0022-1503
  • [Journal-full-title] The Journal of heredity
  • [ISO-abbreviation] J. Hered.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Proteins
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92. Yang H, Kong L, Zhao JS: [DNA damage induced by methyl tertiary-butyl ether in vivo and in vitro]. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi; 2005 Oct;23(5):362-5
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  • OBJECTIVE: To investigate the mechanism of methyl tertiary-butyl ether (MTBE)-induced animal carcinoma.
  • METHODS: Single cell gel electrophoresis assay (SCGE), DNA cross-links test and unscheduled DNA synthesis (UDS) assay were conducted with cultured rat type II pneumocytes and rat hepatocytes in vitro.
  • Simultaneously, the contents of malondialdehyde (MDA) in homogenates of lung and kidney were determined.
  • MTBE above 0.050 mmol/L caused greater DNA migration in cultured rat type II pneumocytes and rat hepatocytes in vitro (P < 0.05), and also with dose-effect relationship (r(lung) = 0.967, r(liver) = 0.963, P < 0.05)).
  • CONCLUSION: MTBE has some genotoxicity on DNA, and the single strand breaks of cell and lipid peroxidation may be one of the possible mechanism of MTBE-induced hepatic and renal tumors of animal.

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  • (PMID = 16266516.001).
  • [ISSN] 1001-9391
  • [Journal-full-title] Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
  • [ISO-abbreviation] Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Methyl Ethers; 29I4YB3S89 / methyl tert-butyl ether
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93. Wang YJ, Jiang YL, Tang HF, Zhao CZ, Chen JQ: Zl-n-91, a selective phosphodiesterase 4 inhibitor, suppresses inflammatory response in a COPD-like rat model. Int Immunopharmacol; 2010 Feb;10(2):252-8
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  • Chronic obstructive pulmonary disease (COPD) is defined as a disease state characterized by poorly reversible airflow limitation induced by cigarette smoking and/or other noxious particle and gases.
  • Phosphodiesterase (PDE) 4 inhibitors are known to elevated cAMP concentrations in inflammatory cells, leading to inhibition of inflammatory response, relaxation of smooth muscle in the airway, and modulation of sensory nerves in the lung as well.
  • To investigate whether Zl-n-91, a new selective PDE4 inhibitor, could decrease inflammation and improve lung function in a COPD-like rat model, male Sprague-Dawley rats are used to challenge with lipopolysaccharide (LPS) and cigarette smoking (CS) exposure to induce COPD-like animal model.
  • Administration of Zl-n-91 at different dosages results in decreases of inflammatory cell in bronchoalveolar lavage fluid (BALF) as compared with vehicle treatment.
  • [MeSH-major] Anti-Inflammatory Agents, Non-Steroidal / therapeutic use. Furans / therapeutic use. Phenyl Ethers / therapeutic use. Phosphodiesterase 4 Inhibitors. Phosphodiesterase Inhibitors / therapeutic use. Pulmonary Disease, Chronic Obstructive / drug therapy
  • [MeSH-minor] Animals. Cyclic Nucleotide Phosphodiesterases, Type 4 / immunology. Disease Models, Animal. Lipopolysaccharides / immunology. Lung / drug effects. Lung / immunology. Lung / physiopathology. Male. Matrix Metalloproteinase 9 / immunology. Matrix Metalloproteinase Inhibitors. Neutrophils / drug effects. Neutrophils / immunology. Peroxidase / drug effects. Peroxidase / immunology. Rats. Rats, Sprague-Dawley. Smoking / adverse effects. Smoking / immunology

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  • [Copyright] Copyright 2009 Elsevier B.V. All rights reserved.
  • (PMID = 19914404.001).
  • [ISSN] 1878-1705
  • [Journal-full-title] International immunopharmacology
  • [ISO-abbreviation] Int. Immunopharmacol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Anti-Inflammatory Agents, Non-Steroidal; 0 / Furans; 0 / Lipopolysaccharides; 0 / Matrix Metalloproteinase Inhibitors; 0 / Phenyl Ethers; 0 / Phosphodiesterase 4 Inhibitors; 0 / Phosphodiesterase Inhibitors; 0 / Zl-n-91 compound; EC 1.11.1.7 / Peroxidase; EC 3.1.4.17 / Cyclic Nucleotide Phosphodiesterases, Type 4; EC 3.4.24.35 / Matrix Metalloproteinase 9
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94. Iwasaki A, Shirakusa T: [Review and evaluation of limited resection for early lung cancer]. Nihon Geka Gakkai Zasshi; 2005 Jun;106(6):385-9
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  • [Title] [Review and evaluation of limited resection for early lung cancer].
  • Limited resection for early lung cancer has been associated with significantly higher local recurrence rates based on previous reports such as those from lung cancer study groups.
  • On the other hand, a few groups demonstrated that patients with small peripheral cancer who undergo limited resection have comparable survival rates with those who undergo lobectomy.
  • Since the introduction of the adenocarcinoma classification by Noguchi surgery for localized bronchioloalveolar carcinoma has focused on limited resection.
  • Caution is necessary when performing wedge resection even if 10 mm or less in diameter and in compromised segmentectomy for early lung cancer.
  • Although limited resection is still controversial intentional segmentectomy for localized bronchioloalveolar carcinoma or less than 20 mm or less in diameter may be recommended without evidence-based medicine.
  • New therapeutic modalities for limited surgery for small-sized lung cancer may increase patient life expectancy.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / surgery. Lung Neoplasms / surgery. Pneumonectomy / methods

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  • (PMID = 15977581.001).
  • [ISSN] 0301-4894
  • [Journal-full-title] Nihon Geka Gakkai zasshi
  • [ISO-abbreviation] Nihon Geka Gakkai Zasshi
  • [Language] jpn
  • [Publication-type] English Abstract; Journal Article; Review
  • [Publication-country] Japan
  • [Number-of-references] 20
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95. Kiyamova R, Gryshkova V, Ovcharenko G, Lituyev D, Malyuchik S, Usenko V, Khozhayenko Y, Gurtovyy V, Yin B, Ritter G, Old L, Filonenko V, Gout I: Development of monoclonal antibodies specific for the human sodium-dependent phosphate co-transporter NaPi2b. Hybridoma (Larchmt); 2008 Aug;27(4):277-84
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  • Inactivation of NaPi2b function by mutations has been linked to human pathologies, such as pulmonary alveolar microlithiasis.
  • These properties make them particularly valuable reagents for elucidating NaPi2b function in health and disease.
  • [MeSH-minor] Animals. Antibody Specificity. Carcinoma / metabolism. Cells, Cultured. Female. Humans. Hybridomas / immunology. Hybridomas / metabolism. Immunoassay / methods. Mice. Mice, Inbred BALB C. Ovarian Neoplasms / metabolism. Ovary / metabolism. Sensitivity and Specificity. Transfection

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  • (PMID = 18724815.001).
  • [ISSN] 1557-8348
  • [Journal-full-title] Hybridoma (2005)
  • [ISO-abbreviation] Hybridoma (Larchmt)
  • [Language] eng
  • [Publication-type] Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / SLC34A2 protein, human; 0 / Sodium-Phosphate Cotransporter Proteins, Type IIb
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96. Cupp JS, Wrede JE, Cherry AM, Arber DA, George TI: Chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type and bronchogenic adenocarcinoma. Appl Immunohistochem Mol Morphol; 2008 May;16(3):296-300
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  • [Title] Chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type and bronchogenic adenocarcinoma.
  • We present the chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma and bronchogenic adenocarcinoma with bronchioloalveolar features.

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  • (PMID = 18301237.001).
  • [ISSN] 1533-4058
  • [Journal-full-title] Applied immunohistochemistry & molecular morphology : AIMM
  • [ISO-abbreviation] Appl. Immunohistochem. Mol. Morphol.
  • [Language] ENG
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Immunoglobulin Heavy Chains; 0 / Neoplasm Proteins; EC 3.4.22.- / Caspases; EC 3.4.22.- / MALT1 protein, human
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97. Tischer BK, Smith GA, Osterrieder N: En passant mutagenesis: a two step markerless red recombination system. Methods Mol Biol; 2010;634:421-30
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  • Bacterial artificial chromosomes are used to maintain and modify large sequences of different origins in Escherichia coli.
  • Since foreign sequences, such as antibiotic resistance genes as well as frt- or loxP-sites are often unwanted in mutant BAC clones, we developed a Red-based technique that allows for the scarless generation of point mutations, deletions, and insertion of smaller and larger sequences.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial

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  • (PMID = 20677001.001).
  • [ISSN] 1940-6029
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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98. Xian HQ, Werth K, Gottlieb DI: Promoter analysis in ES cell-derived neural cells. Biochem Biophys Res Commun; 2005 Feb 4;327(1):155-62
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  • [Title] Promoter analysis in ES cell-derived neural cells.
  • Neural cells derived from ES cells in cell culture (ESNCs) have many of the properties of normal neural cells and provide a model of "neurogenesis-in-a-dish."
  • ES cells are transfected with bacterial artificial chromosomes (BACs) containing Olig2, a gene with a key role in neural fate choice.
  • One BAC is modified by recombineering to insert a reporter gene and a gene for selecting stably transfected clones.
  • Another BAC contains a deletion of a suspected Olig2 promoter.
  • The combination of ESNCs and BAC recombineering will have broad application for analyzing gene transcription in the nervous system and will be applicable to human ES cells.
  • The general approach should also be applicable to the many other cell lineages that can now be derived from mouse and human ES cells in culture.
  • [MeSH-minor] Animals. Basic Helix-Loop-Helix Transcription Factors. Cell Differentiation. Cell Line. Chromosomes, Artificial, Bacterial / genetics. Gene Expression. Mice. Nerve Tissue Proteins / genetics. Phenotype. Sequence Deletion / genetics. Transgenes / genetics. Up-Regulation / genetics

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  • (PMID = 15629444.001).
  • [ISSN] 0006-291X
  • [Journal-full-title] Biochemical and biophysical research communications
  • [ISO-abbreviation] Biochem. Biophys. Res. Commun.
  • [Language] eng
  • [Grant] United States / NINDS NIH HHS / NS / NS045809; United States / NINDS NIH HHS / NS / P01 NS39577-03
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Basic Helix-Loop-Helix Transcription Factors; 0 / Nerve Tissue Proteins; 0 / Olig2 protein, mouse
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99. Orvieto E, Maiorano E, Bottiglieri L, Maisonneuve P, Rotmensz N, Galimberti V, Luini A, Brenelli F, Gatti G, Viale G: Clinicopathologic characteristics of invasive lobular carcinoma of the breast: results of an analysis of 530 cases from a single institution. Cancer; 2008 Oct 1;113(7):1511-20
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  • [Title] Clinicopathologic characteristics of invasive lobular carcinoma of the breast: results of an analysis of 530 cases from a single institution.
  • BACKGROUND: Although invasive lobular carcinoma (ILC) is the second most common histotype of breast cancer, the prognostic implications of its clinicopathologic characteristics remain controversial.
  • METHODS: The authors undertook a retrospective analysis of a large series of cases treated and followed at a single institution, with the objective of assessing the prognostic/predictive value of distinct clinicopathologic features of the tumors, after revision of the original histopathologic preparations and statistical analyses.
  • RESULTS: Overall, 530 patients with pure ILC (57% with the classic type; 19% with the alveolar type; 11% with the solid type; and the remaining 13% characterized by pleomorphic, signet ring cell, histiocytoid, or apocrine features) were included in the study.
  • Patients with ILC of the 'nonclassic' subtype demonstrated significantly increased breast-related events (hazards ratio of 1.80; 95% confidence interval, 1.04-3.10) and a trend toward reduced disease-free survival and overall survival.
  • [MeSH-major] Breast Neoplasms / pathology. Carcinoma, Lobular / pathology

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  • (PMID = 18704988.001).
  • [ISSN] 0008-543X
  • [Journal-full-title] Cancer
  • [ISO-abbreviation] Cancer
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] United States
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100. Shao CW, Chen SL, Scheuring CF, Xu JY, Sha ZX, Dong XL, Zhang HB: Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes. Mar Biotechnol (NY); 2010 Oct;12(5):558-68
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  • [Title] Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes.
  • Two bacterial artificial chromosome (BAC) libraries for C. semilaevis, with large, high-quality inserts and deep coverage, were constructed in the BamHI and HindIII sites of the vector pECBAC1.
  • The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents.
  • Thus, the two BAC libraries of C. semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
  • [MeSH-major] Chromosomes, Artificial, Bacterial / genetics. Cloning, Molecular. Fisheries / methods. Flatfishes / genetics. Gene Expression Regulation / genetics. Gene Library. Sex Determination Processes / genetics

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  • (PMID = 19957095.001).
  • [ISSN] 1436-2236
  • [Journal-full-title] Marine biotechnology (New York, N.Y.)
  • [ISO-abbreviation] Mar. Biotechnol.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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