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1. Yokoyama KK, Murata T, Pan J, Nakade K, Kishikawa S, Ugai H, Kimura M, Kujime Y, Hirose M, Masuzaki S, Yamasaki T, Kurihara C, Okubo M, Nakano Y, Kusa Y, Yoshikawa A, Inabe K, Ueno K, Obata Y: Genetic materials at the gene engineering division, RIKEN BioResource Center. Exp Anim; 2010;59(2):115-24
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  • The genetic resources include cloned DNAs, gene libraries (e.g., cDNA and genomic DNA cloned into phage, cosmid, BAC, phosmid, and YAC), vectors, hosts, recombinant viruses, and ordered library sets derived from animal cells, including human and mouse cells, microorganisms, and viruses.

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  • (PMID = 20484845.001).
  • [ISSN] 1881-7122
  • [Journal-full-title] Experimental animals
  • [ISO-abbreviation] Exp. Anim.
  • [Language] ENG
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Review
  • [Publication-country] Japan
  • [Number-of-references] 34
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2. Palti Y, Rodriguez MF, Gahr SA, Purcell MK, Rexroad CE 3rd, Wiens GD: Identification, characterization and genetic mapping of TLR1 loci in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol; 2010 May-Jun;28(5-6):918-26
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  • Two TLR1 loci were identified from a rainbow trout bacterial artificial chromosome (BAC) library using DNA sequencing and genetic linkage analyses.
  • Full length cDNA clone and direct sequencing of four BACs revealed an intact omTLR1 open reading frame (ORF) located on chromosome 14 and a second locus on chromosome 25 that contains a TLR1 pseudogene.
  • However, due to the lack of TLR6 and 10 genes in teleost genomes and up-regulation of TLR1 mRNA in response to LPS and bacterial infection in other fish species we hypothesize an important role for omTLR1 in anti-microbial immunity.
  • Therefore, the identification of a TLR2 ortholog in rainbow trout and the development of assays to measure ligand binding and downstream signaling are critical for future elucidation of omTLR1 functions.

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  • [Copyright] Published by Elsevier Ltd.
  • (PMID = 20153434.001).
  • [ISSN] 1095-9947
  • [Journal-full-title] Fish & shellfish immunology
  • [ISO-abbreviation] Fish Shellfish Immunol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Messenger; 0 / Toll-Like Receptor 1
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3. Saul SM, Brzezinski JA 4th, Altschuler RA, Shore SE, Rudolph DD, Kabara LL, Halsey KE, Hufnagel RB, Zhou J, Dolan DF, Glaser T: Math5 expression and function in the central auditory system. Mol Cell Neurosci; 2008 Jan;37(1):153-69
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  • The basic helix-loop-helix (bHLH) transcription factor Math5 (Atoh7) is required for retinal ganglion cell (RGC) and optic nerve development.
  • Math5 expression in the CN was verified by mRNA in situ hybridization, and the identity of positive neurons was confirmed morphologically using a Math5-Cre BAC transgene with an alkaline phosphatase reporter.
  • These temporal changes are consistent with a higher-level auditory processing disorder involving the CN, potentially affecting the integration of binaural sensory information.

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  • (PMID = 17977745.001).
  • [ISSN] 1044-7431
  • [Journal-full-title] Molecular and cellular neurosciences
  • [ISO-abbreviation] Mol. Cell. Neurosci.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM067840-04; United States / NIDDK NIH HHS / DK / P60 DK20572; United States / NIDCD NIH HHS / DC / R01 DC04825; United States / NEI NIH HHS / EY / T32 EY013934; United States / NIGMS NIH HHS / GM / T32 GM07544; United States / NIDCD NIH HHS / DC / P30 DC005188; United States / NIGMS NIH HHS / GM / R01 GM067840; United States / NIDDK NIH HHS / DK / P60 DK020572; United States / NEI NIH HHS / EY / R01 EY14259; United States / NEI NIH HHS / EY / R01 EY014259-03; United States / NEI NIH HHS / EY / EY014259-03; United States / NIGMS NIH HHS / GM / GM067840-04; United States / NIDCD NIH HHS / DC / T32 DC00011; United States / NIGMS NIH HHS / GM / T32 GM007544; United States / NEI NIH HHS / EY / R01 EY014259; United States / NIDCD NIH HHS / DC / P30 DC05188; United States / NIDCD NIH HHS / DC / T32 DC000011; United States / NIDCD NIH HHS / DC / R01 DC004825
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt; 0 / Atoh7 protein, mouse; 0 / Basic Helix-Loop-Helix Transcription Factors; 0 / Nerve Tissue Proteins; 0 / RNA, Messenger; 0 / Stilbamidines; EC 3.2.1.23 / beta-Galactosidase; G34N38R2N1 / Bromodeoxyuridine
  • [Other-IDs] NLM/ NIHMS38894; NLM/ PMC2266824
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4. Wester U, Bondeson ML, Edeby C, Annerén G: Clinical and molecular characterization of individuals with 18p deletion: a genotype-phenotype correlation. Am J Med Genet A; 2006 Jun 1;140(11):1164-71
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The aims of present study were to clinically and molecularly characterize the syndrome further in seven subjects with de novo 18p deletions and to perform genotype-phenotype correlation.
  • All seven subjects had terminal deletions and no interstitial deletion was observed with subtelomeric FISH analyses.
  • To define the extent of the 18p deletions and the parental origin of the deletion microsatellite- and FISH analyses were performed on genomic DNA and on lymphoblastoid cell lines of the study participants.
  • Totally 19 chromosomes, 18 specific polymorphic microsatellite markers, and 5 BAC clones were used.

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  • [Copyright] Copyright 2006 Wiley-Liss, Inc.
  • (PMID = 16691587.001).
  • [ISSN] 1552-4825
  • [Journal-full-title] American journal of medical genetics. Part A
  • [ISO-abbreviation] Am. J. Med. Genet. A
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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5. Inamura K, Togashi Y, Nomura K, Ninomiya H, Hiramatsu M, Satoh Y, Okumura S, Nakagawa K, Ishikawa Y: let-7 microRNA expression is reduced in bronchioloalveolar carcinoma, a non-invasive carcinoma, and is not correlated with prognosis. Lung Cancer; 2007 Dec;58(3):392-6
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  • [Title] let-7 microRNA expression is reduced in bronchioloalveolar carcinoma, a non-invasive carcinoma, and is not correlated with prognosis.
  • It has been proposed that reduced let-7 expression causes RAS expression and correlates with poor survival of lung cancer cases, but little is known about correlations with clinicopathologic features.
  • In this study, we examined 15 early bronchioloalveolar carcinomas (BACs), usually considered as adenocarcinomas in situ, as well as 26 well-differentiated and 25 less-differentiated invasive adenocarcinomas, to assess the association between tumor progression and let-7 expression levels.
  • Additionally, we investigated 47 invasive lung adenocarcinomas for EGFR and KRAS mutations and correlations with let-7 levels.
  • Relative to the corresponding normal lung tissue, reduced let-7 expression was observed in 13 of 15 BACs (87%) and totally in 52 of the 66 adenocarcinomas (79%), suggesting a link with early occurrence in carcinogenesis.
  • On classification of adenocarcinomas into two groups according to let-7 expression, no prognostic or genetic differences were observed.
  • Interestingly, some differences between histological subtypes were observed, such as lower let-7 expression levels in acinar adenocarcinomas and mucinous BACs.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / genetics. Adenocarcinoma, Bronchiolo-Alveolar / pathology. Gene Expression Regulation, Neoplastic / genetics. MicroRNAs / genetics

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  • [CommentIn] Lung Cancer. 2008 May;60(2):307 [18395292.001]
  • (PMID = 17728006.001).
  • [ISSN] 0169-5002
  • [Journal-full-title] Lung cancer (Amsterdam, Netherlands)
  • [ISO-abbreviation] Lung Cancer
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / MicroRNAs; 0 / mirnlet7 microRNA, human
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6. Conner JA, Goel S, Gunawan G, Cordonnier-Pratt MM, Johnson VE, Liang C, Wang H, Pratt LH, Mullet JE, DeBarry J, Yang L, Bennetzen JL, Klein PE, Ozias-Akins P: Sequence analysis of bacterial artificial chromosome clones from the apospory-specific genomic region of Pennisetum and Cenchrus. Plant Physiol; 2008 Jul;147(3):1396-411
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  • [Title] Sequence analysis of bacterial artificial chromosome clones from the apospory-specific genomic region of Pennisetum and Cenchrus.
  • Thirty-two ASGR bacterial artificial chromosomes (BACs) isolated from both species and one ASGR-recombining BAC from P. squamulatum, which together cover approximately 2.7 Mb of DNA, were used to investigate the genomic structure of this region.
  • Phrap assembly of 4,521 high-quality reads generated 1,341 contiguous sequences (contigs; 730 from the ASGR and 30 from the ASGR-recombining BAC in P. squamulatum, plus 580 from the C. ciliaris ASGR).
  • These putative coding regions were further analyzed in silico with reference to the rice (Oryza sativa) and sorghum (Sorghum bicolor) genomes using the resources at Gramene (www.gramene.org) and Phytozome (www.phytozome.net) and by hybridization against sorghum BAC filters.
  • [MeSH-minor] Arabidopsis Proteins / genetics. Chromosomes, Artificial, Bacterial. Cloning, Molecular. DNA Transposable Elements. Gene Duplication. Genome, Plant. Molecular Sequence Data. Oryza / genetics. Sequence Analysis, DNA. Sorghum / genetics. Synteny. Transcription Factors / genetics

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  • (PMID = 18508959.001).
  • [ISSN] 0032-0889
  • [Journal-full-title] Plant physiology
  • [ISO-abbreviation] Plant Physiol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ ED544199/ ED548719/ EU559277/ EU559280
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Arabidopsis Proteins; 0 / BABY BOOM protein, Arabidopsis; 0 / DNA Transposable Elements; 0 / Transcription Factors
  • [Other-IDs] NLM/ PMC2442526
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7. van Duin M, van Marion R, Watson JE, Paris PL, Lapuk A, Brown N, Oseroff VV, Albertson DG, Pinkel D, de Jong P, Nacheva EP, Dinjens W, van Dekken H, Collins C: Construction and application of a full-coverage, high-resolution, human chromosome 8q genomic microarray for comparative genomic hybridization. Cytometry A; 2005;63(1):10-9
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  • [Title] Construction and application of a full-coverage, high-resolution, human chromosome 8q genomic microarray for comparative genomic hybridization.
  • METHODS: A minimal tiling path contig of 702 8q-specific bacterial artificial chromosome (BAC) clones was generated with a novel computational tool (BAC Contig Assembler).
  • BAC clones were amplified by degenerative oligonucleotide primer (DOP) polymerase chain reaction and subsequently printed onto glass slides.
  • For validation of the array DNA samples of gastroesophageal and prostate cancer cell lines, and chronic myeloid leukemia specimens were used, which were previously characterized by multicolor fluorescence in situ hybridization and conventional CGH.
  • The 8q array was further tested with paraffin-embedded prostate cancer specimens.
  • In comparison with conventional CGH, the resolution of the detected changes was much improved, which was demonstrated by an amplicon of 0.7 Mb and a deletion of 0.6 Mb, both spanned by only six BAC clones.
  • [MeSH-minor] Adenocarcinoma / genetics. Cardia. Chromosome Aberrations. Fixatives. Formaldehyde. Humans. In Situ Hybridization, Fluorescence. Male. Nucleic Acid Hybridization. Oligonucleotide Array Sequence Analysis. Prostatic Neoplasms / genetics. Stomach Neoplasms / genetics. Tumor Cells, Cultured

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  • (PMID = 15619731.001).
  • [ISSN] 1552-4922
  • [Journal-full-title] Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • [ISO-abbreviation] Cytometry A
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / CA89520
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Fixatives; 1HG84L3525 / Formaldehyde
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8. Xie SG, Shi DW, Wen DH, Wang R, Xi DL: Control of bromate and THM precursors using ozonation combined system. Biomed Environ Sci; 2007 Jun;20(3):217-25
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  • The biological activated carbon (BAC) filter could effectively reduce CHCl3FP and CHCl2BrFP, but increase CHClBr2FP.

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  • (PMID = 17672213.001).
  • [ISSN] 0895-3988
  • [Journal-full-title] Biomedical and environmental sciences : BES
  • [ISO-abbreviation] Biomed. Environ. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Bromates; 0 / Trihalomethanes; 4R7X1O2820 / Chlorine; 66H7ZZK23N / Ozone
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9. Boross P, Breukel C, van Loo PF, van der Kaa J, Claassens JW, Bujard H, Schönig K, Verbeek JS: Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector. Genesis; 2009 Nov;47(11):729-35
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  • [Title] Highly B lymphocyte-specific tamoxifen inducible transgene expression of CreER T2 by using the LC-1 locus BAC vector.
  • The generation of cell type specific inducible Cre transgenic mice is the most challenging and limiting part in the development of spatio-temporally controlled knockout mouse models.
  • Here we report the generation and characterization of a B lymphocyte-specific tamoxifen-inducible Cre transgenic mouse strain, LC-1-hCD19-CreER(T2).
  • [MeSH-major] B-Lymphocytes / metabolism. Chromosomes, Artificial, Bacterial. Gene Expression Regulation / drug effects. Integrases / genetics. Tamoxifen / pharmacology. Transgenes

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  • [Copyright] (c) 2009 Wiley-Liss, Inc.
  • (PMID = 19621440.001).
  • [ISSN] 1526-968X
  • [Journal-full-title] Genesis (New York, N.Y. : 2000)
  • [ISO-abbreviation] Genesis
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, CD19; 0 / DNA Primers; 094ZI81Y45 / Tamoxifen; EC 2.7.7.- / Cre recombinase; EC 2.7.7.- / Integrases
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10. Shintani Y, Ohta M, Iwasaki T, Ikeda N, Tomita E, Nagano T, Kawahara K: A case of micronodular pneumocyte hyperplasia diagnosed through surgical resection. Ann Thorac Cardiovasc Surg; 2010 Aug;16(1):45-7
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  • Micronodular pneumocyte hyperplasia (MNPH) is often associated with tuberous sclerosis complex and/or lymphangioleiomyomatosis.
  • We present the case of a 45-year-old woman with MNPH without evidence of either.
  • A preoperative high-resolution chest computed topographic scan demonstrated a ground-glass opacity 8 mm in diameter that revealed the possibility of atypical adenomatous hyperplasia (AAH) or bronchioloalveolar carcinoma (BAC).
  • Therefore an S3 segmentectomy of the right lung was performed, and the specimens revealed the characteristic histological and immunohistological features of MNPH.
  • Solitary MNPH is extremely rare and requires to be distinguished from AAH or BAC on a computed tomographic scan; therefore surgical resection may be required to definitely rule out malignancy.
  • [MeSH-major] Pneumocytes / pathology. Solitary Pulmonary Nodule / diagnosis
  • [MeSH-minor] Biopsy. Diagnosis, Differential. Female. Humans. Hyperplasia. Immunohistochemistry. Middle Aged. Predictive Value of Tests. Tomography, X-Ray Computed

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  • (PMID = 20190710.001).
  • [ISSN] 2186-1005
  • [Journal-full-title] Annals of thoracic and cardiovascular surgery : official journal of the Association of Thoracic and Cardiovascular Surgeons of Asia
  • [ISO-abbreviation] Ann Thorac Cardiovasc Surg
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] Japan
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11. Findley SD, Cannon S, Varala K, Du J, Ma J, Hudson ME, Birchler JA, Stacey G: A fluorescence in situ hybridization system for karyotyping soybean. Genetics; 2010 Jul;185(3):727-44
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  • The development of a universal soybean (Glycine max [L.
  • We used genetically anchored BAC clones both to identify individual chromosomes in metaphase spreads and to complete a FISH-based karyotyping cocktail that permitted simultaneous identification of all 20 chromosome pairs.
  • These studies led to the identification and characterization of a reciprocal chromosome translocation between chromosomes 11 and 13 in two accessions of wild soybean.
  • [MeSH-minor] Base Sequence. Chromosomes, Artificial, Bacterial. DNA, Plant. Molecular Sequence Data. Nucleic Acid Hybridization

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  • (PMID = 20421607.001).
  • [ISSN] 1943-2631
  • [Journal-full-title] Genetics
  • [ISO-abbreviation] Genetics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Plant
  • [Other-IDs] NLM/ PMC2907198
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12. Buchanan W, Roddick F, Porter N: Removal of VUV pre-treated natural organic matter by biologically activated carbon columns. Water Res; 2008 Jul;42(13):3335-42
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  • The system uses sufficient VUV radiation (16J cm(-2)) to significantly enhance the production of biologically degradable moieties prior to treatment with biologically activated carbon (BAC).
  • The VUV-BAC process decreased the overall dissolved organic carbon (DOC) concentration of a natural water sample by 54% and 44% for the virgin carbon and previously used BAC, respectively.
  • Furthermore, VUV-BAC treatment decreased the trihalomethane (THM) formation potential (THMFP) by 60-70% and the haloacetic acid (HAA) formation potential (HAAFP) by 74%.
  • The BAC systems effectively removed the hydrogen peroxide residual produced by VUV irradiation.
  • Although nitrite formation can result from VUV treatment of natural organic matter (NOM), none was detected before or after BAC treatment.

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  • (PMID = 18502470.001).
  • [ISSN] 0043-1354
  • [Journal-full-title] Water research
  • [ISO-abbreviation] Water Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Biological Products; 0 / Nitrates; 0 / Organic Chemicals; 7440-44-0 / Carbon; BBX060AN9V / Hydrogen Peroxide
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13. Ou SH, Zell JA: Validation study of the proposed IASLC staging revisions of the T4 and M non-small cell lung cancer descriptors using data from 23,583 patients in the California Cancer Registry. J Thorac Oncol; 2008 Mar;3(3):216-27
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  • [Title] Validation study of the proposed IASLC staging revisions of the T4 and M non-small cell lung cancer descriptors using data from 23,583 patients in the California Cancer Registry.
  • BACKGROUND: We performed a validation study of the proposed International Association for the Study of Lung Cancer (IASLC) tumor, node, metastasis (TNM) and stage grouping revisions on advanced nonbronchioloalveolar carcinoma (non-BAC) non-small cell lung cancer (NSCLC).
  • METHODS: Twenty-three thousand five hundred eighty-three patients from the California Cancer Registry between 1999 and 2003 with histologically confirmed non-BAC NSCLC and complete TNM staging were identified and reclassified according to the IASLC proposed TNM revisions and new stage groupings.
  • Surveillance, Epidemiology, and End Results extent of disease codes were used to identify various T4 and M descriptors.
  • T4 due to additional nodules had significant survival advantage over other T4 and M descriptors among non-BAC NSCLC and individual histology and warrants down-staging to T3.
  • CONCLUSIONS: IASLC has greatly improved the T4 and M descriptors allowing better prognostication of advanced non-BAC NSCLC.
  • [MeSH-major] Carcinoma, Non-Small-Cell Lung / secondary. Heart Neoplasms / secondary. Lung Neoplasms / pathology. Neoplasm Staging / methods. Pleural Neoplasms / secondary. Registries / statistics & numerical data

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  • (PMID = 18317063.001).
  • [ISSN] 1556-1380
  • [Journal-full-title] Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
  • [ISO-abbreviation] J Thorac Oncol
  • [Language] eng
  • [Grant] United States / NCCDPHP CDC HHS / DP / 1U58DP00807-01; United States / NCI NIH HHS / PC / N01-PC-35136; United States / NCI NIH HHS / PC / N01-PC-35139; United States / NCI NIH HHS / PC / N01-PC-54404
  • [Publication-type] Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.; Validation Studies
  • [Publication-country] United States
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14. Devillard E, McIntosh FM, Newbold CJ, Wallace RJ: Rumen ciliate protozoa contain high concentrations of conjugated linoleic acids and vaccenic acid, yet do not hydrogenate linoleic acid or desaturate stearic acid. Br J Nutr; 2006 Oct;96(4):697-704
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  • In incubations with ruminal microbial fractions (bacterial fraction (BAC), protozoal fraction (PRO)), LA metabolism was very similar in strained ruminal fluid (SRF) and in the BAC, while the PRO had LA-metabolising activity an order of magnitude lower.
  • The absence of an alternative route of VA/CLA formation via desaturation of stearate was confirmed by incubations of SRF, BAC or PRO with [14C]stearate.

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  • (PMID = 17010229.001).
  • [ISSN] 0007-1145
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Linoleic Acids, Conjugated; 0 / Oleic Acids; 0 / Stearic Acids; 143-25-9 / 11-octadecenoic acid; 4ELV7Z65AP / stearic acid; 9KJL21T0QJ / Linoleic Acid
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15. Yue W, Miao Y, Li X, Wu X, Zhao A, Nakagaki M: Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system. Appl Microbiol Biotechnol; 2006 Nov;73(1):181-6
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  • [Title] Cloning and expression of manganese superoxide dismutase of the silkworm, Bombyx mori by Bac-to-Bac/BmNPV Baculovirus expression system.
  • In this paper, we used the total fat body RNA of silkworm, Bombyx mori L. to clone and sequence a 648-bp Mn-SOD cDNA fragment through RT-PCR.
  • Furthermore, a newly established Bac-to-Bac/BmNPV Baculovirus expression system was used to overexpress the recombinant Mn-SOD enzyme in silkworm larvae.

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  • (PMID = 16804693.001).
  • [ISSN] 0175-7598
  • [Journal-full-title] Applied microbiology and biotechnology
  • [ISO-abbreviation] Appl. Microbiol. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Complementary; 63231-63-0 / RNA; EC 1.15.1.1 / Superoxide Dismutase
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16. Uchikov A, Batashki I, Dimitrov I, Uchikova E, Belovezhdov V, Bonev P: [Operative treatment and remote results in patients with bronchoalveolar lung cancer]. Khirurgiia (Sofiia); 2008;(1-2):18-9
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  • [Title] [Operative treatment and remote results in patients with bronchoalveolar lung cancer].
  • Bronchoalveolar pulmonary carcinoma (BAC) is a type of adenocarcinoma with an increasing frequency.
  • Because BAC rarely metastasize outside the thorax, the postoperative results are good.
  • MATERIAL AND METHOD: We present 28 patients with BAC, operated in the clinic for a period of 7 years--12 patients with an infiltrative form of BAC, 16 patients with nodular form RESULTS: 12 patients underwent lobectomy, 15 patients--pneumonectomy, and 1 patient--bilobectomy.
  • CONCLUSIONS: We found a better 5-years survival rate for the patients with BAC in comparison with the other histological types of non-small cell pulmonary carcinoma
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / surgery. Lung Neoplasms / surgery. Pulmonary Surgical Procedures / methods
  • [MeSH-minor] Adult. Aged. Disease-Free Survival. Female. Humans. Male. Middle Aged. Neoplasm Staging

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  • (PMID = 18985897.001).
  • [ISSN] 0450-2167
  • [Journal-full-title] Khirurgii︠a︡
  • [ISO-abbreviation] Khirurgiia (Sofiia)
  • [Language] bul
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Bulgaria
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17. Výborný P, Sejcková L: [Antiglaucoma drugs--content of preservatives and the patient's compliance]. Cesk Slov Oftalmol; 2006 Jul;62(4):270-2, 274
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  • The authors compare the benzalconium chloride (BAC) concentrations in individual drug used for glaucoma treating.
  • The daily dose of BAC was calculated according to the drop's size and BAC concentration.
  • The daily dose of BAC differs depending on the selected therapy markedly--doses in the range from 0 to 10 micrograms were found.
  • The BAC concentration is one of important factors affecting the patient's compliance in the treatment of the primary open angle glaucoma.

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  • (PMID = 16895060.001).
  • [ISSN] 1211-9059
  • [Journal-full-title] Ceská a slovenská oftalmologie : casopis Ceské oftalmologické spolecnosti a Slovenské oftalmologické spolecnosti
  • [ISO-abbreviation] Cesk Slov Oftalmol
  • [Language] cze
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Czech Republic
  • [Chemical-registry-number] 0 / Benzalkonium Compounds; 0 / Ophthalmic Solutions; 0 / Preservatives, Pharmaceutical
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18. Dar M, Giesler T, Richardson R, Cai C, Cooper M, Lavasani S, Kille P, Voet T, Vermeesch J: Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions. BMC Biotechnol; 2008;8:86
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  • [Title] Development of a novel ozone- and photo-stable HyPer5 red fluorescent dye for array CGH and microarray gene expression analysis with consistent performance irrespective of environmental conditions.
  • In high resolution array CGH experiments, HyPer5 is demonstrated to detect chromosomal aberrations at loci 2p21-16.3 and 15q26.3-26.2 from three patient sample using bacterial artificial chromosome (BAC) arrays.

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  • [ISSN] 1472-6750
  • [Journal-full-title] BMC biotechnology
  • [ISO-abbreviation] BMC Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Carbocyanines; 0 / DNA, Complementary; 0 / Fluorescent Dyes; 66H7ZZK23N / Ozone
  • [Other-IDs] NLM/ PMC2613886
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19. Gu X, Greiner ER, Mishra R, Kodali R, Osmand A, Finkbeiner S, Steffan JS, Thompson LM, Wetzel R, Yang XW: Serines 13 and 16 are critical determinants of full-length human mutant huntingtin induced disease pathogenesis in HD mice. Neuron; 2009 Dec 24;64(6):828-40
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  • [Title] Serines 13 and 16 are critical determinants of full-length human mutant huntingtin induced disease pathogenesis in HD mice.
  • The N-terminal 17 amino acids of huntingtin (NT17) can be phosphorylated on serines 13 and 16; however, the significance of these modifications in Huntington's disease pathogenesis remains unknown.
  • In this study, we developed BAC transgenic mice expressing full-length mutant huntingtin (fl-mhtt) with serines 13 and 16 mutated to either aspartate (phosphomimetic or SD) or alanine (phosphoresistant or SA).
  • However, fl-mhtt-induced disease pathogenesis, including motor and psychiatric-like behavioral deficits, mhtt aggregation, and selective neurodegeneration are abolished in SD but preserved in SA mice.
  • Together, our findings demonstrate that serines 13 and 16 are critical determinants of fl-mhtt-induced disease pathogenesis in vivo, supporting the targeting of huntingtin NT17 domain and its modifications in HD therapy.


20. Yamamoto R, Uenishi H, Yasue H, Takagaki Y, Sato E: The genomic structure and a novel alternatively spliced form of porcine pTalpha chain. Mol Immunol; 2007 Jan;44(4):591-7
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  • A complete genomic nucleotide sequence for porcine pTalpha gene was obtained from a BAC clone, which revealed a novel exon 2 missing in human and murine counterparts.
  • Using thymocyte RNA and RT-PCR, three types of porcine pTalpha-chain cDNA sequences, pTalpha1, pTalpha2 and pTalpha3, were obtained.
  • Using RT-PCR, pTalpha3 appeared expressed predominantly in the thymocyte RNA.
  • [MeSH-major] Alternative Splicing. Genome. Membrane Glycoproteins / genetics. Receptors, Antigen, T-Cell, alpha-beta / genetics. Swine / genetics

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  • (PMID = 16712934.001).
  • [ISSN] 0161-5890
  • [Journal-full-title] Molecular immunology
  • [ISO-abbreviation] Mol. Immunol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Membrane Glycoproteins; 0 / Receptors, Antigen, T-Cell, alpha-beta; 0 / pre-T cell receptor alpha
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21. Qiao Y, Liu X, Harvard C, Nolin SL, Brown WT, Koochek M, Holden JJ, Lewis ME, Rajcan-Separovic E: Large-scale copy number variants (CNVs): distribution in normal subjects and FISH/real-time qPCR analysis. BMC Genomics; 2007;8:167
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  • In order to understand their potential significance and to facilitate interpretation of array-CGH findings in constitutional disorders and cancers, we studied 27 normal individuals (9 Caucasian; 9 African American; 9 Hispanic) using commercially available 1 Mb resolution BAC array (Spectral Genomics).
  • A selection of CNVs was further analyzed by FISH and real-time quantitative PCR (RT-qPCR).
  • FISH analysis of 6 CNVs (4 previously reported and 2 novel CNVs) in normal subjects resulted in the confirmation of copy number changes for 1 of 2 novel CNVs and 2 of 4 known CNvs. Three CNVs tested by FISH were further validated by RT-qPCR and comparable data were obtained.
  • This included the lack of copy number change by both RT-qPCR and FISH for clone RP11-100C24, one of the most common known copy number variants, as well as confirmation of deletions for clones RP11-89M16 and RP5-1011O17.
  • Further study of a small selection of CNVs indicated concordant and discordant array vs. FISH/RT-qPCR results.

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  • (PMID = 17565693.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Other-IDs] NLM/ PMC1920519
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22. Kerstens HH, Kollers S, Kommadath A, Del Rosario M, Dibbits B, Kinders SM, Crooijmans RP, Groenen MA: Mining for single nucleotide polymorphisms in pig genome sequence data. BMC Genomics; 2009;10:4
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  • Available BAC and BAC-end sequences and their naming and mapping information, all obtained from SangerInstitute FTP site, served as a rough assembly of a reference genome.
  • [MeSH-minor] Animals. Chromosomes, Artificial, Bacterial. Cluster Analysis. Genetic Markers. Sequence Alignment. Sequence Analysis, DNA / methods

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  • (PMID = 19126189.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Genetic Markers
  • [Other-IDs] NLM/ PMC2637895
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23. Song J, Dong H, Ma C, Zhao B, Shang G: Construction and functional characterization of an integrative form lambda Red recombineering Escherichia coli strain. FEMS Microbiol Lett; 2010 Aug 1;309(2):178-83
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  • LS-GR shows high recombination efficiency for medium copy number vector and single copy number BAC vector modifications.

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  • (PMID = 20618864.001).
  • [ISSN] 1574-6968
  • [Journal-full-title] FEMS microbiology letters
  • [ISO-abbreviation] FEMS Microbiol. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Recombinases; 0 / Viral Proteins
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24. Cheung SW, Shaw CA, Scott DA, Patel A, Sahoo T, Bacino CA, Pursley A, Li J, Erickson R, Gropman AL, Miller DT, Seashore MR, Summers AM, Stankiewicz P, Chinault AC, Lupski JR, Beaudet AL, Sutton VR: Microarray-based CGH detects chromosomal mosaicism not revealed by conventional cytogenetics. Am J Med Genet A; 2007 Aug 1;143A(15):1679-86
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  • We have developed a clinically validated, targeted BAC clone array as a platform for comparative genomic hybridization (aCGH) to enable detection of a wide range of pathologic copy number changes in DNA.
  • This enhanced ability of aCGH to detect mosaicism missed by routine chromosome analysis may be due to some combination of testing multiple cell lineages and/or failure of cytogenetically abnormal T lymphocytes to respond to mitogens.

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  • [Copyright] (c) 2007 Wiley-Liss, Inc.
  • (PMID = 17607705.001).
  • [ISSN] 1552-4833
  • [Journal-full-title] American journal of medical genetics. Part A
  • [ISO-abbreviation] Am. J. Med. Genet. A
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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25. Eggermann T, Krause-Plonka I, Wollmann HA, Zerres K, Dai G, Meyer E, Bartsch O: Supernumerary marker chromosome 7 and maternal uniparental disomy 7 in a boy with growth retardation and triangular face. Clin Dysmorphol; 2006 Jan;15(1):9-12
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  • Using fluorescence in situ hybridization and BAC clones, the supernumerary marker chromosome was found to be a highly deleted chromosome 7 with breakpoints within the pericentric euchromatin (partial trisomy 7).
  • Considering that the SMC(7) contributed very little, if any, to the phenotype of this boy, we propose that UPD7 studies should be carried out in children with pre- and postnatal growth retardation of unknown cause even in the absence of a SMC.

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  • (PMID = 16317300.001).
  • [ISSN] 0962-8827
  • [Journal-full-title] Clinical dysmorphology
  • [ISO-abbreviation] Clin. Dysmorphol.
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Euchromatin
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26. Swiercz R, Hałatek T, Majcherek W, Grzelińiska Z, Wasowicz W: [Toxic effect of benzalkonium chloride on animals and humans]. Med Pr; 2007;58(2):139-42
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  • Benzalkonium chloride (BAC) exerts toxic effects on microorganisms.
  • Various BAC-containing preparations used by people may induce a number of adverse effects on the human body.
  • Bearing in mind that BAC is widely used in different branches of the national economy, its toxic effect may cause a health problem of significant importance to humans.
  • The authors describe BAC toxic effects exerted on humans and laboratory animals as well as relevant hazards resulting from the use of BAC-contained preparations.

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  • (PMID = 17926503.001).
  • [ISSN] 0465-5893
  • [Journal-full-title] Medycyna pracy
  • [ISO-abbreviation] Med Pr
  • [Language] pol
  • [Publication-type] English Abstract; Journal Article; Review
  • [Publication-country] Poland
  • [Chemical-registry-number] 0 / Benzalkonium Compounds; 0 / Preservatives, Pharmaceutical
  • [Number-of-references] 25
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27. Summers RJ, Shehata BM, Bleacher JC, Stockwell C, Rapkin L: Mucinous adenocarcinoma of the lung in association with congenital pulmonary airway malformation. J Pediatr Surg; 2010 Nov;45(11):2256-9
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  • [Title] Mucinous adenocarcinoma of the lung in association with congenital pulmonary airway malformation.
  • Congenital pulmonary airway malformation (CPAM) is a rare developmental abnormality of the lung that has been associated with the presence of rhabdomyosarcoma, pleuropulmonary blastoma, and most commonly bronchioalveolar carcinoma (BAC) of the lung.
  • Here, we report the case of an 8-year-old patient who developed KRAS mutation positive stage IV mucinous adenocarcinoma of the lung in association with CPAM.
  • This case reflects the previously recognized progression of CPAM to malignancy and suggests that BAC arising in CPAM may take a more aggressive course than previously recognized.
  • [MeSH-major] Adenocarcinoma, Mucinous / diagnosis. Cystic Adenomatoid Malformation of Lung, Congenital / diagnosis. Lung Neoplasms / diagnosis. Precancerous Conditions
  • [MeSH-minor] Bronchoscopy. Child. Diagnosis, Differential. Female. Humans. Pneumonectomy / methods. Tomography, X-Ray Computed

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  • [Copyright] Copyright © 2010 Elsevier Inc. All rights reserved.
  • (PMID = 21034957.001).
  • [ISSN] 1531-5037
  • [Journal-full-title] Journal of pediatric surgery
  • [ISO-abbreviation] J. Pediatr. Surg.
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] United States
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28. Dunnick WA, Collins JT, Shi J, Westfield G, Fontaine C, Hakimpour P, Papavasiliou FN: Switch recombination and somatic hypermutation are controlled by the heavy chain 3' enhancer region. J Exp Med; 2009 Nov 23;206(12):2613-23
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  • Both class switch recombination (CSR) and somatic hypermutation (SHM) require transcription and the trans-acting factor activation-induced cytidine deaminase (AID), and must be up-regulated during antigen-dependent differentiation of B lymphocytes.
  • To test the role of the heavy chain 3' enhancers in both CSR and SHM, we used a BAC transgene of the entire heavy chain constant region locus.
  • Using Cre-loxP recombination to delete a 28-kb region that contains the four known 3' heavy chain enhancers, we isolated lines of BAC transgenic mice with an intact heavy chain locus and paired lines in the same chromosomal insertion site lacking the 3' enhancers.

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  • (PMID = 19887393.001).
  • [ISSN] 1540-9538
  • [Journal-full-title] The Journal of experimental medicine
  • [ISO-abbreviation] J. Exp. Med.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / P30 CA046592; United States / NIAID NIH HHS / AI / AI076057; United States / NCI NIH HHS / CA / CA46592; United States / NCI NIH HHS / CA / R01 CA098495; United States / NIAID NIH HHS / AI / R01 AI076057; United States / NCI NIH HHS / CA / CA098495
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 3' Untranslated Regions; 0 / Immunoglobulin Heavy Chains; EC 2.7.7.- / Cre recombinase; EC 2.7.7.- / Integrases; EC 3.5.4.5 / Cytidine Deaminase
  • [Other-IDs] NLM/ PMC2806627
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29. Liu H, Jiang H, Haltli B, Kulowski K, Muszynska E, Feng X, Summers M, Young M, Graziani E, Koehn F, Carter GT, He M: Rapid cloning and heterologous expression of the meridamycin biosynthetic gene cluster using a versatile Escherichia coli-streptomyces artificial chromosome vector, pSBAC. J Nat Prod; 2009 Mar 27;72(3):389-95
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  • Herein we describe the development of a versatile Escherichia coli-Streptomyces shuttle Bacterial Artificial Chromosomal (BAC) conjugation vector, pSBAC, to facilitate the cloning, genetic manipulation, and heterologous expression of actinomycetes secondary metabolite biosynthetic gene clusters.
  • The success of heterologous expression of such a giant gene cluster demonstrates the versatility of BAC cloning technology and paves the road for future exploration of expression of the meridamycin biosynthetic pathway in various hosts, including strains that have been optimized for polyketide production.
  • [MeSH-major] Escherichia coli / genetics. Genes, Bacterial / genetics. Macrolides / chemical synthesis. Macrolides / metabolism. Polyketide Synthases / metabolism. Streptomyces / genetics
  • [MeSH-minor] Chromosomes, Artificial. Chromosomes, Artificial, Bacterial / metabolism. Cloning, Molecular. Genetic Vectors / chemical synthesis. Genetic Vectors / genetics. Molecular Structure

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  • (PMID = 19191550.001).
  • [ISSN] 1520-6025
  • [Journal-full-title] Journal of natural products
  • [ISO-abbreviation] J. Nat. Prod.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 3-normeridamycin; 0 / Macrolides; 0 / meridamycin; 79956-01-7 / Polyketide Synthases
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30. Petersohn JD, Conquergood LR, Leung M: Acute histologic effects and thermal distribution profile of disc biacuplasty using a novel water-cooled bipolar electrode system in an in vivo porcine model. Pain Med; 2008 Jan-Feb;9(1):26-32
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  • Disc biacuplasty (D-BAC) is a novel procedure that uses two water-cooled radiofrequency electrodes in a bipolar configuration to heat a large volume of the posterior annulus fibrosus.
  • METHODS: Seven porcine lumbar discs were treated with D-BAC to assess acute effects on the treated tissue in a "worst-case"in vivo model.

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  • (PMID = 18254764.001).
  • [ISSN] 1526-2375
  • [Journal-full-title] Pain medicine (Malden, Mass.)
  • [ISO-abbreviation] Pain Med
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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31. Duchesne A, Eggen A: Radiation hybrid mapping of genes and newly identified microsatellites in candidate regions for bovine arthrogryposis-palatoschisis and progressive ataxia based on comparative data from man, mouse and rat. J Anim Breed Genet; 2005 Apr;122 Suppl 1:28-35
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  • Primer pairs were designed for 15 loci around each candidate gene, in a region of about 20 megabases and were used to screen a bovine Bacterial Artificial Chromosome (BAC) library.
  • Eighteen microsatellites were found in the identified BAC clones, 11 on BTA24 and seven on BTA16.
  • [MeSH-major] Arthrogryposis / veterinary. Ataxia / veterinary. Cattle Diseases / genetics. Cattle Diseases / pathology. Genetic Predisposition to Disease / genetics
  • [MeSH-minor] Animals. Cattle. Chromosomes, Artificial, Bacterial. DNA Primers. Humans. Mice. Microsatellite Repeats / genetics. Radiation Hybrid Mapping / veterinary. Rats


32. Chen YP, Tseng CP, Liaw LL, Wang CL, Chen IC, Wu WJ, Wu MD, Yuan GF: Cloning and characterization of monacolin K biosynthetic gene cluster from Monascus pilosus. J Agric Food Chem; 2008 Jul 23;56(14):5639-46
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  • In the present study, a bacterial artificial chromosome (BAC) clone, mps01, was screened from the BAC library constructed from Monascus pilosus BCRC38072 genomic DNA.
  • [MeSH-minor] Amino Acid Sequence. Chromosomes, Artificial, Bacterial. Molecular Sequence Data. Phylogeny. Polyketide Synthases / genetics. Polyketide Synthases / metabolism

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  • (PMID = 18578535.001).
  • [ISSN] 1520-5118
  • [Journal-full-title] Journal of agricultural and food chemistry
  • [ISO-abbreviation] J. Agric. Food Chem.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 79956-01-7 / Polyketide Synthases; 9LHU78OQFD / Lovastatin
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33. Chattoo JP, Stevens MP, Nair V: Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis. J Virol Methods; 2006 Aug;135(2):288-91
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  • [Title] Rapid identification of non-essential genes for in vitro replication of Marek's disease virus by random transposon mutagenesis.
  • Marek's disease virus (MDV) is a highly oncogenic alphaherpesvirus that induces rapid-onset T-cell lymphomas in poultry.
  • The construction of an infectious bacterial artificial chromosome (BAC) clone of the highly oncogenic RB-1B strain of MDV has been described previously.
  • Virus reconstituted from the BAC clone induced rapid-onset lymphomas in chickens very similar to the wildtype viruses.
  • In this paper, the construction of a high-density random transposon-insertion mutant library of the RB-1B BAC clone using a high throughput in vitro transposon mutagenesis technique is described.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial. Open Reading Frames

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  • (PMID = 16650486.001).
  • [ISSN] 0166-0934
  • [Journal-full-title] Journal of virological methods
  • [ISO-abbreviation] J. Virol. Methods
  • [Language] eng
  • [Grant] United Kingdom / Biotechnology and Biological Sciences Research Council / / BBS/B/10544
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA Transposable Elements
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34. Park EJ, Kim H, Kim Y, Yi J, Choi K, Park K: Inflammatory responses may be induced by a single intratracheal instillation of iron nanoparticles in mice. Toxicology; 2010 Sep 10;275(1-3):65-71
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  • Magnetite iron nanoparticles have been widely used as contrast agents and in thermal therapy for cancer.
  • When mice were treated with iron oxide nanoparticles (250 microg/kg, 500 microg/kg, and 1mg/kg) by a single intratracheal instillation, the level of intracellular reduced glutathione (GSH) was decreased in the cells of bronchoalveolar lavage (BAL) fluid.
  • The arrest of cell cycles in G1 phase was observed, but S-phase was significantly decreased.
  • Formation of microgranuloma, which is one of the indicators for chronic inflammatory response, was observed in the alveolar space.
  • In addition, distribution of B cell and CD8+ T cell in blood lymphocytes was increased at day 28.
  • [MeSH-major] Ferric Compounds / toxicity. Inflammation Mediators / toxicity. Lung / drug effects. Lung / metabolism. Nanoparticles / toxicity. Trachea / drug effects

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  • [Copyright] (c) 2010 Elsevier Ireland Ltd. All rights reserved.
  • (PMID = 20540983.001).
  • [ISSN] 1879-3185
  • [Journal-full-title] Toxicology
  • [ISO-abbreviation] Toxicology
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Ferric Compounds; 0 / Inflammation Mediators; 1K09F3G675 / ferric oxide
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35. Maver A, Medica I, Salobir B, Tercelj-Zorman M, Sabovic M, Petrovic D, Peterlin B: Peroxisome proliferator-activated receptor gamma/Pro12Ala polymorphism and peroxisome proliferator-activated receptor gamma coactivator-1 alpha/Gly482Ser polymorphism in patients with sarcoidosis. Sarcoidosis Vasc Diffuse Lung Dis; 2008 Sep;25(1):29-35
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  • BACKGROUND AND AIM OF THE WORK: Reduced expression and activity of the peroxisome proliferator-activated receptor gamma (PPARG) have been measured in cells of bronchoalveolar lavage fluid in sarcoidosis patients.

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  • (PMID = 19070258.001).
  • [ISSN] 1124-0490
  • [Journal-full-title] Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG
  • [ISO-abbreviation] Sarcoidosis Vasc Diffuse Lung Dis
  • [Language] ENG
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] Italy
  • [Chemical-registry-number] 0 / Heat-Shock Proteins; 0 / PPAR gamma; 0 / PPARGC1A protein, human; 0 / Transcription Factors; 9007-49-2 / DNA
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36. Dominguez-Ventura A, Allen MS, Cassivi SD, Nichols FC 3rd, Deschamps C, Pairolero PC: Lung cancer in octogenarians: factors affecting morbidity and mortality after pulmonary resection. Ann Thorac Surg; 2006 Oct;82(4):1175-9
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  • [Title] Lung cancer in octogenarians: factors affecting morbidity and mortality after pulmonary resection.
  • BACKGROUND: Predictors of morbidity and mortality after pulmonary resection for lung cancer in patients 80 years of age or older are unknown.
  • METHODS: The medical records of all patients 80 years of age or older who had pulmonary resection for lung cancer from January 1985 through September 2004 were reviewed.
  • The cancer was squamous cell carcinoma in 143 patients (37.7%), adenocarcinoma in 166 (43.8%), bronchoalveolar cell carcinoma in 47 (12.4%), and other in 23 (6.1%).
  • CONCLUSIONS: Pulmonary resection for lung cancer in octogenarians is feasible.
  • [MeSH-major] Adenocarcinoma / surgery. Carcinoma, Squamous Cell / surgery. Lung Neoplasms / surgery. Pneumonectomy / statistics & numerical data

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  • (PMID = 16996903.001).
  • [ISSN] 1552-6259
  • [Journal-full-title] The Annals of thoracic surgery
  • [ISO-abbreviation] Ann. Thorac. Surg.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Netherlands
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37. Hahn C, Islamian AP, Renz H, Nockher WA: Airway epithelial cells produce neurotrophins and promote the survival of eosinophils during allergic airway inflammation. J Allergy Clin Immunol; 2006 Apr;117(4):787-94
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  • BACKGROUND: Eosinophil-epithelial cell interactions make a major contribution to asthmatic airway inflammation.
  • Neurotrophin levels are increased in bronchoalveolar lavage fluid during allergic asthma.
  • OBJECTIVE: We sought to investigate the role of neurotrophins as inflammatory mediators in eosinophil-epithelial cell interactions during the allergic immune response.
  • METHODS: Neurotrophin expression in the lung was investigated by means of immunohistochemistry and ELISA in a mouse model of chronic experimental asthma.
  • Coculture experiments were performed with airway epithelial cells and bronchoalveolar lavage fluid eosinophils.
  • RESULTS: Neurotrophin levels increased continuously during chronic allergic airway inflammation, and airway epithelial cells were the major source of NGF and BDNF within the inflamed lung.
  • Lung eosinophils expressed the BDNF and NGF receptors tropomyosin-related kinase (Trk) A and TrkB, and coculture with airway epithelial cells resulted in enhanced epithelial neurotrophin production, as well as in prolonged survival of eosinophils.
  • [MeSH-minor] Animals. Asthma / etiology. Asthma / pathology. Asthma / physiopathology. Base Sequence. Brain-Derived Neurotrophic Factor / biosynthesis. Cell Survival. Cytokines / pharmacology. Epithelial Cells / drug effects. Epithelial Cells / physiology. Female. Inflammation Mediators / metabolism. Mice. Mice, Inbred BALB C. Models, Biological. RNA, Messenger / genetics. RNA, Messenger / metabolism. Receptor, trkA / biosynthesis. Receptor, trkB / biosynthesis

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  • (PMID = 16630935.001).
  • [ISSN] 0091-6749
  • [Journal-full-title] The Journal of allergy and clinical immunology
  • [ISO-abbreviation] J. Allergy Clin. Immunol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Brain-Derived Neurotrophic Factor; 0 / Cytokines; 0 / Inflammation Mediators; 0 / Nerve Growth Factors; 0 / RNA, Messenger; EC 2.7.10.1 / Receptor, trkA; EC 2.7.10.1 / Receptor, trkB
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38. Bruno AC, Baffa O, Carneiro AO: Hybrid system for magnetic and acoustic measurement. Conf Proc IEEE Eng Med Biol Soc; 2009;2009:761-4
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  • In order to improve the spatial resolution of Biosusceptometry of Alternate Current (BAC), we are suggesting the coupling of a Doppler ultrasonic transducer with the BAC system.

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  • (PMID = 19964487.001).
  • [ISSN] 1557-170X
  • [Journal-full-title] Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
  • [ISO-abbreviation] Conf Proc IEEE Eng Med Biol Soc
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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39. Liu HZ, Yang HZ, Mi S, Cui B, Hua F, Hu ZW: Toll like receptor 2 mediates bleomycin-induced acute lung injury, inflammation and fibrosis in mice. Yao Xue Xue Bao; 2010 Aug;45(8):976-86
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  • [Title] Toll like receptor 2 mediates bleomycin-induced acute lung injury, inflammation and fibrosis in mice.
  • Anti-cancer drug bleomycin (BLM) can cause acute lung injury (ALI) which often results in pulmonary fibrosis due to a failure of resolving acute inflammatory response.
  • TLR2 inhibition attenuated BLM-induced increase of inflammatory cells in bronchoalveolar lavage fluid (BALF), and reversed the immunosuppressive microenvironment by enhancing TH1 response (P<0.05) and inhibiting TH2 (P<0.001), Treg (P<0.01) and TH17 (P<0.01) responses.
  • [MeSH-major] Acute Lung Injury / metabolism. Cytokines / secretion. Inflammation / metabolism. Pulmonary Fibrosis / metabolism. Toll-Like Receptor 2 / metabolism
  • [MeSH-minor] Animals. Bleomycin / toxicity. Bronchoalveolar Lavage Fluid. Cells, Cultured. Dendritic Cells / cytology. Dendritic Cells / metabolism. Interleukin-17 / secretion. Interleukin-23 / secretion. Interleukin-6 / secretion. Lung / metabolism. MAP Kinase Signaling System. Male. Mice. Mice, Inbred C57BL. T-Lymphocytes, Regulatory / drug effects. Th1 Cells / drug effects. Th2 Cells / drug effects

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  • (PMID = 21348427.001).
  • [ISSN] 0513-4870
  • [Journal-full-title] Yao xue xue bao = Acta pharmaceutica Sinica
  • [ISO-abbreviation] Yao Xue Xue Bao
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Cytokines; 0 / Interleukin-17; 0 / Interleukin-23; 0 / Interleukin-6; 0 / Tlr2 protein, mouse; 0 / Toll-Like Receptor 2; 11056-06-7 / Bleomycin
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40. Honey D, Caylor C, Luthi R, Kerrigan S: Comparative alcohol concentrations in blood and vitreous fluid with illustrative case studies. J Anal Toxicol; 2005 Jul-Aug;29(5):365-9
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  • The vitreous alcohol concentration (VAC) exceeded the blood alcohol concentration (BAC) in 209 cases (71%).
  • In casework where the VAC > BAC, linear regression analysis indicated an R2 value of 0.958 (n = 209) and a VAC approximately 16% higher than the BAC.
  • The VAC/BAC ratio was more variable at lower BACs (< 0.1 g/100 mL).
  • Although VAC/BAC ratios were more consistent at concentrations of 0.1 g/100 mL and above, the overall ratio ranged from 1.01 to 2.20.
  • Of the 81 cases where BAC > VAC, a total of 24 cases indicated no vitreous alcohol.
  • Unlike the VAC/BAC data set which consisted of 97% femoral blood, the source of blood in the BAC > VAC data set was slightly more variable.
  • Of the 81 cases where BAC > VAC the blood source consisted of femoral (n = 68), heart (n = 8), pleural cavity (n = 2), carotid (n = 1), jugular (n = 1), and chest blood (n = 1).

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  • (PMID = 16105262.001).
  • [ISSN] 0146-4760
  • [Journal-full-title] Journal of analytical toxicology
  • [ISO-abbreviation] J Anal Toxicol
  • [Language] eng
  • [Publication-type] Case Reports; Comparative Study; Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 3K9958V90M / Ethanol
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41. Xu F, Cheng DH, Di YF, Tan K, Li LY, Lu GX, Tan YQ: [Identification of a cryptic 1p36.3 microdeletion in a patient with Prader-Willi-like syndrome features]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi; 2010 Oct;27(5):524-9
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  • [Title] [Identification of a cryptic 1p36.3 microdeletion in a patient with Prader-Willi-like syndrome features].
  • OBJECTIVE: To determine the karyotype of a patient with Prader-Willi-like syndrome features.
  • RESULTS: No abnormality was discovered by high resolution karyotype analysis and methylation-specific PCR studies.
  • The deleted region was shown within a 4.2 Mb in the distal 1p by 3 BAC FISH probes of 1p36 combined with real-time PCR technique.
  • Family pedigree investigation showed the chromosome abnormality was de novo.

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  • (PMID = 20931530.001).
  • [ISSN] 1003-9406
  • [Journal-full-title] Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics
  • [ISO-abbreviation] Zhonghua Yi Xue Yi Chuan Xue Za Zhi
  • [Language] chi
  • [Publication-type] Case Reports; English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
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42. Reddy S, Dolzhanskaya N, Krogh J, Velinov M: A novel 1.4 Mb de novo microdeletion of chromosome 1q21.3 in a child with microcephaly, dysmorphic features and mental retardation. Eur J Med Genet; 2009 Nov-Dec;52(6):443-5
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  • [Title] A novel 1.4 Mb de novo microdeletion of chromosome 1q21.3 in a child with microcephaly, dysmorphic features and mental retardation.
  • About 1 Mb deletion in the chromosomal region 1q21.3 was identified using BAC array CGH analysis.

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  • (PMID = 19772933.001).
  • [ISSN] 1878-0849
  • [Journal-full-title] European journal of medical genetics
  • [ISO-abbreviation] Eur J Med Genet
  • [Language] eng
  • [Publication-type] Case Reports; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] Netherlands
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43. Burtey S, Riera M, Ribe E, Pennekamp P, Passage E, Rance R, Dworniczak B, Fontés M: Overexpression of PKD2 in the mouse is associated with renal tubulopathy. Nephrol Dial Transplant; 2008 Apr;23(4):1157-65
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  • Polycystin-2 (PC-2), a cation channel of the Trp family, is involved in autosomal dominant polycystic kidney disease (ADPKD) type 2 (ADPKD2).
  • In the present paper, we describe a mouse model for human PC-2 overexpression, obtained by inserting a human bacterial artificial chromosome (BAC) containing the PKD2 gene.
  • [MeSH-minor] Animals. Blotting, Western. Disease Models, Animal. Extracellular Matrix Proteins / biosynthesis. Extracellular Matrix Proteins / genetics. Female. Humans. In Situ Hybridization, Fluorescence. Male. Mice. Mice, Inbred C57BL. Mice, Transgenic. Reverse Transcriptase Polymerase Chain Reaction. Severity of Illness Index

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  • (PMID = 18048422.001).
  • [ISSN] 1460-2385
  • [Journal-full-title] Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association
  • [ISO-abbreviation] Nephrol. Dial. Transplant.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Extracellular Matrix Proteins; 0 / TRPP Cation Channels; 0 / polycystic kidney disease 2 protein; 63231-63-0 / RNA
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44. Nguyen RL, Tam LW, Lefebvre PA: The LF1 gene of Chlamydomonas reinhardtii encodes a novel protein required for flagellar length control. Genetics; 2005 Mar;169(3):1415-24
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  • Here we describe the positional cloning and molecular characterization of the LF1 gene using a bacterial artificial chromosome (BAC) library.
  • Rescue of the lf1 phenotype upon transformation was obtained with clones containing the complete LF1 gene as well as clones that lack the last two exons of the gene, indicating that only the amino-terminal portion of the LF1 gene product (LF1p) is required for function.
  • Although LF1 helps regulate flagellar length, the LF1p localizes almost exclusively in the cell body, with <1% of total cellular LF1p localizing to the flagella.

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  • (PMID = 15489537.001).
  • [ISSN] 0016-6731
  • [Journal-full-title] Genetics
  • [ISO-abbreviation] Genetics
  • [Language] ENG
  • [Databank-accession-numbers] GENBANK/ AY298951
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM034437; United States / NIGMS NIH HHS / GM / GM-34437
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Protozoan; 0 / Protozoan Proteins; 0 / RNA, Protozoan
  • [Other-IDs] NLM/ PMC1449559
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45. Na Z, Huipeng Y, Lipan L, Cuiping C, Umashankar ML, Xingmeng L, Xiaofeng W, Bing W, Weizheng C, Cenis JL: Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system. Appl Microbiol Biotechnol; 2008 Feb;78(2):221-6
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  • [Title] Efficient production of canine interferon-alpha in silkworm Bombyx mori by use of a BmNPV/Bac-to-Bac expression system.
  • The recombinant baculovirus harboring canine interferon gene was rapidly generated by the BmNPV/Bac-to-Bac system that was recently developed.
  • In B. mori-derived cell lines, the expression of the recombinant protein reached maximal levels around 72-96 h post-infection.
  • [MeSH-minor] Animals. Baculoviridae / genetics. Cell Line. Cell Survival. Chromatography, Affinity. Dogs. Electrophoresis, Polyacrylamide Gel. Genetic Vectors. Glycosylation. Larva / genetics. Larva / metabolism. Molecular Sequence Data. Rhabdoviridae Infections / immunology. Sequence Analysis, DNA. Vesiculovirus / immunology

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  • (PMID = 18066544.001).
  • [ISSN] 0175-7598
  • [Journal-full-title] Applied microbiology and biotechnology
  • [ISO-abbreviation] Appl. Microbiol. Biotechnol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ EF080825
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Interferon-alpha; 0 / Recombinant Proteins
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46. Aokage K, Ishii G, Yoshida J, Hishida T, Nishimura M, Nagai K, Ochiai A: Histological progression of small intrapulmonary metastatic tumor from primary lung adenocarcinoma. Pathol Int; 2010 Dec;60(12):765-73
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  • [Title] Histological progression of small intrapulmonary metastatic tumor from primary lung adenocarcinoma.
  • Histological typing based on the World Health Organization classification (bronchioloalveolar carcinoma, acinar, papillary, and solid subtype) was used to evaluate 234 metastases from the primary lung adenocarcinomas of 139 patients.
  • The predominant subtype of metastasis 3 mm or less in diameter was bronchioloalveolar carcinoma when the primary lesion was diagnosed as predominant bronchioloalveolar carcinoma, acinar, and papillary subtype.
  • These findings suggest that implanted cancer cells display lepidic growth in the early metastatic phase and recapitulate the morphological heterogeneity of the original tumor as the metastasis enlarges.
  • [MeSH-major] Adenocarcinoma / secondary. Lung Neoplasms / pathology. Lung Neoplasms / secondary. Neoplasm Metastasis / pathology
  • [MeSH-minor] Aged. Disease Progression. Female. Humans. Male. Middle Aged. Neoplasm Staging

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  • [Copyright] © 2010 The Authors. Pathology International © 2010 Japanese Society of Pathology and Blackwell Publishing Asia Pty Ltd.
  • (PMID = 21091834.001).
  • [ISSN] 1440-1827
  • [Journal-full-title] Pathology international
  • [ISO-abbreviation] Pathol. Int.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Australia
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47. Semba S, Iwaya K, Matsubayashi J, Serizawa H, Kataba H, Hirano T, Kato H, Matsuoka T, Mukai K: Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung. Clin Cancer Res; 2006 Apr 15;12(8):2449-54
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  • [Title] Coexpression of actin-related protein 2 and Wiskott-Aldrich syndrome family verproline-homologous protein 2 in adenocarcinoma of the lung.
  • PURPOSE: Highly invasive and metastatic cancer cells, such as adenocarcinoma of the lung cells, form irregular protrusions by assembling a branched network of actin filaments.
  • In mammalian cells, the actin-related protein 2 and 3 (Arp2/3) complex initiates actin assembly to form lamellipodial protrusions by binding to Wiskott-Aldrich syndrome (WASP)/WASP family verproline-homologous protein 2 (WAVE2).
  • In this study, colocalization of Arp2 and WAVE2 in adenocarcinoma of the lung was investigated to elucidate its prognostic value.
  • EXPERIMENTAL DESIGN: Immunohistochemical staining of Arp2 and WAVE2 was done on mirror sections of 115 adenocarcinomas of the lung from pathologic stage IA to IIIA classes.
  • Kaplan-Meier disease-free survival and overall survival curves were analyzed to determine the prognostic significance of the coexpression of Arp2 and WAVE2.
  • RESULTS: Immunoreactivity for both Arp2 and WAVE2 was detected in the same cancer cells in 78 (67.8%) of the 115 lung cancer specimens.
  • The proportion of cancer cells expressing both Arp2 and WAVE2 was significantly higher in cases with lymph-node metastasis (P = 0.0046), and significantly lower in bronchioloalveolar carcinomas (P < 0.0001).
  • The patients whose cancer cells coexpressed them had a shorter disease-free survival time (P < 0.0001) and overall survival time (P < 0.0001).
  • CONCLUSIONS: Coexpression of Arp2 and WAVE2 is correlated with poorer patient outcome, and may be involved in the mechanism of cancer metastasis.
  • [MeSH-major] Actin-Related Protein 2 / biosynthesis. Adenocarcinoma / pathology. Lung Neoplasms / pathology. Wiskott-Aldrich Syndrome Protein Family / biosynthesis
  • [MeSH-minor] Aged. Disease-Free Survival. Female. Humans. Immunohistochemistry. Male. Multivariate Analysis. Neoplasm Staging. Prognosis. Survival Analysis

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  • (PMID = 16638851.001).
  • [ISSN] 1078-0432
  • [Journal-full-title] Clinical cancer research : an official journal of the American Association for Cancer Research
  • [ISO-abbreviation] Clin. Cancer Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / ACTR2 protein, human; 0 / Actin-Related Protein 2; 0 / WASF2 protein, human; 0 / Wiskott-Aldrich Syndrome Protein Family
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48. Chen L, Li M, Li Q, Yang X, An X, Chen Y: Characterization of the bovine immunoglobulin lambda light chain constant IGLC genes. Vet Immunol Immunopathol; 2008 Aug 15;124(3-4):284-94
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  • To characterize the bovine immunoglobulin lambda light chain constant region (IGLC) genes, we have isolated a bacterial artificial chromosome (BAC) clone by a PCR based approach from a bovine genomic DNA library, constructed using a genital ridge cell line derived from a male Holstein fetus.
  • The positive BAC clone, containing the bovine IGLC genes, was fully sequenced and had a 138 kb insert.
  • This conclusion was further confirmed by a series of RT-PCR amplifications, which also showed that among these four genes the IGLC3 was preferentially expressed in cattle.
  • [MeSH-minor] Amino Acid Sequence. Animals. Base Sequence. Chromosomes, Artificial, Bacterial. Cloning, Molecular. DNA / chemistry. DNA / genetics. Gene Library. Immunoglobulin Joining Region / genetics. Immunoglobulin Joining Region / immunology. Male. Molecular Sequence Data. Phylogeny. Polymerase Chain Reaction / veterinary. Sequence Alignment

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  • (PMID = 18538861.001).
  • [ISSN] 0165-2427
  • [Journal-full-title] Veterinary immunology and immunopathology
  • [ISO-abbreviation] Vet. Immunol. Immunopathol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Immunoglobulin Constant Regions; 0 / Immunoglobulin Joining Region; 0 / Immunoglobulin Light Chains; 0 / Immunoglobulin lambda-Chains; 9007-49-2 / DNA
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49. Emond MR, Jontes JD: Inhibition of protocadherin-alpha function results in neuronal death in the developing zebrafish. Dev Biol; 2008 Sep 1;321(1):175-87
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  • The pcdhalpha/CNR gene comprises a diverse array of neuronal cell-surface proteins of the cadherin superfamily, although very little is known about their role in neural development.
  • Using whole-mount immunocytochemistry and BAC-based expression of Pcdh1alpha-GFP fusion proteins, we find that Pcdh1alpha does not appear to form stable, synaptic puncta at early stages of synaptogenesis.
  • This cell death phenotype can be attenuated by the expression of a soluble Pcdh1alpha cytoplasmic domain.
  • [MeSH-minor] Animals. Cell Death. Central Nervous System / cytology. Central Nervous System / embryology. Embryo, Nonmammalian / metabolism. Protein Structure, Tertiary

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  • (PMID = 18602383.001).
  • [ISSN] 1095-564X
  • [Journal-full-title] Developmental biology
  • [ISO-abbreviation] Dev. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Cadherins
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50. Williams LE, Detter C, Barry K, Lapidus A, Summers AO: Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing. Appl Environ Microbiol; 2006 Jul;72(7):4899-906
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  • Sequencing of the large (>50 kb), low-copy-number (<5 per cell) plasmids that mediate horizontal gene transfer has been hindered by the difficulty and expense of isolating DNA from individual plasmids of this class.
  • We report here that a kit method previously devised for purification of bacterial artificial chromosomes (BACs) can be adapted for effective preparation of individual plasmids up to 220 kb from wild gram-negative and gram-positive bacteria.
  • Adaptation of this BAC method for large plasmid isolation removes one major technical hurdle to expanding our knowledge of the natural plasmid gene pool.
  • [MeSH-major] DNA, Bacterial / isolation & purification. Genetic Techniques. Genome, Bacterial / genetics. Plasmids / isolation & purification. Sequence Analysis, DNA
  • [MeSH-minor] Chromosomes, Artificial, Bacterial / genetics. Corynebacterium / genetics. Escherichia coli / genetics. Magnetics. Microspheres. Molecular Sequence Data. Staphylococcus / genetics

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  • (PMID = 16820486.001).
  • [ISSN] 0099-2240
  • [Journal-full-title] Applied and environmental microbiology
  • [ISO-abbreviation] Appl. Environ. Microbiol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ DQ364638/ DQ390454/ DQ390455/ DQ390456/ DQ390457/ DQ390458
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Bacterial
  • [Other-IDs] NLM/ PMC1489313
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51. Ingvardsen CR, Xing Y, Frei UK, Lübberstedt T: Genetic and physical fine mapping of Scmv2, a potyvirus resistance gene in maize. Theor Appl Genet; 2010 May;120(8):1621-34
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  • We fine mapped Scmv2 to a region of 0.28 cM, covering a physical distance of 1.3426 Mb, and developed six new polymorphic SSR markers based on publicly available BAC sequences within this region.
  • Analysis of the public B73 BAC library as well as the syntenic rice region did not reveal any similarity to known resistance genes.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial. Chromosomes, Plant / genetics. DNA, Plant / metabolism. Expressed Sequence Tags. Genes, Plant. Genetic Markers. Models, Genetic. Mosaic Viruses / genetics. Oryza / genetics. Physical Chromosome Mapping. Plant Diseases / genetics. Polymorphism, Genetic

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  • (PMID = 20155410.001).
  • [ISSN] 1432-2242
  • [Journal-full-title] TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
  • [ISO-abbreviation] Theor. Appl. Genet.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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52. Pedrosa-Harand A, Kami J, Gepts P, Geffroy V, Schweizer D: Cytogenetic mapping of common bean chromosomes reveals a less compartmentalized small-genome plant species. Chromosome Res; 2009;17(3):405-17
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  • Cytogenetic maps of common bean chromosomes 3, 4 and 7 were constructed by fluorescence in-situ hybridization (FISH) of BAC and a few other genomic clones.
  • Although all clones were selected with genetically mapped markers, mostly with single-copy RFLPs, a large subset of BACs, from 13 different genomic regions, contained repetitive sequences, as concluded from the regional distribution patterns of multiple FISH signals on chromosomes: pericentromeric, subtelomeric and dispersed.
  • Pericentromeric repeats were present in all 11 chromosome pairs with different intensities, whereas subtelomeric repeats were present in several chromosome ends, but with different signal intensities depending on the BAC, suggesting that the terminal heterochromatin fraction of this species may be composed of different repeats.
  • [MeSH-minor] Chromosome Mapping. Chromosomes, Artificial, Bacterial. In Situ Hybridization, Fluorescence. Recombination, Genetic

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  • (PMID = 19330455.001).
  • [ISSN] 1573-6849
  • [Journal-full-title] Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
  • [ISO-abbreviation] Chromosome Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
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53. Kaplan S, Prato CG: Impact of BAC limit reduction on different population segments: a Poisson fixed effect analysis. Accid Anal Prev; 2007 Nov;39(6):1146-54
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  • [Title] Impact of BAC limit reduction on different population segments: a Poisson fixed effect analysis.
  • Over the past few decades, several countries enacted the reduction of the legal blood alcohol concentration (BAC) limit, often alongside the administrative license revocation or suspension, to battle drinking-and-driving behavior.
  • The present analysis focuses on the evaluation of the impact of BAC limit reduction on different population segments.
  • Model estimates demonstrate that, for alcohol-related single-vehicle crashes, (i) BAC laws are more effective in terms of reduction of number of casualties rather than number of accidents, (ii) women and elderly population exhibit higher law compliance with respect to men and to young adult and adult population, respectively, and (iii) the presence of passengers in the vehicle enhances the sense of responsibility of the driver.


54. Barnett NP, Wei J, Czachowski C: Measured alcohol content in college party mixed drinks. Psychol Addict Behav; 2009 Mar;23(1):152-6
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  • Standard drink equivalents were calculated and blood alcohol concentrations (BACs) for men and women were estimated.
  • Estimated BACs varied widely depending on drink alcohol concentration, but in most cases a heavy drinking episode for both men and women resulted in an estimated BAC at or above .08.

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  • (PMID = 19290700.001).
  • [ISSN] 0893-164X
  • [Journal-full-title] Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors
  • [ISO-abbreviation] Psychol Addict Behav
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 3K9958V90M / Ethanol
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55. Makushkin EO, Korsunov VM, Pavlova II: [The biomass of microbial communities in different types of alluvial soils in the upper reach of the Selenga River estuary]. Izv Akad Nauk Ser Biol; 2009 Jan-Feb;(1):100-7
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  • The bacterial complex predominated at the beginning of July; actinomycete and spore-fungal complexes, at the end of August; and spore-fungal and actinomycete complexes, at the beginning of October.

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  • (PMID = 19239119.001).
  • [ISSN] 1026-3470
  • [Journal-full-title] Izvestiia Akademii nauk. Seriia biologicheskaia
  • [ISO-abbreviation] Izv. Akad. Nauk. Ser. Biol.
  • [Language] RUS
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Russia (Federation)
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56. Kowalski W, Mierzwa T, Grabiec M, Laskowski R, Walentowicz M: [Problems in diagnosing breast cancer in women younger than 25 years old]. Ginekol Pol; 2006 May;77(5):376-82
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  • [Title] [Problems in diagnosing breast cancer in women younger than 25 years old].
  • OBJECTIVES: There are several challenges in the diagnosis of breast cancer in young women.
  • Special consideration must be given to the woman's fertility and body image issues, but the difficulty in distinguishing benign from malignant lesions in this group of patients still exists.
  • The aim of our study was to show five cases of breast cancer in young women.
  • MATERIALS AND METHODS: Based on 5 cases of breast cancer in women younger than 25, several methods used in diagnosis in this group of patients were discussed.
  • The role of USG and BAC was analyzed.
  • RESULTS: Diagnosing and treatment of young patients with breast cancer should be performed in specialized oncological centers experienced in dealing with breast cancer in very young women.
  • Because of the possibility of breast cancer in women under 25 years old, each case of breast tumor should be precisely diagnosed, especially by ultrasonography and biopsy (BAC) 2.
  • Each case suspected of breast cancer in young women should be diagnosed in highly equipped and specialized medical center.
  • [MeSH-major] Breast Neoplasms / diagnosis
  • [MeSH-minor] Adult. Age Factors. Combined Modality Therapy. Diagnosis, Differential. Female. Humans. Mammography / methods

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  • (PMID = 16958227.001).
  • [ISSN] 0017-0011
  • [Journal-full-title] Ginekologia polska
  • [ISO-abbreviation] Ginekol. Pol.
  • [Language] pol
  • [Publication-type] Case Reports; English Abstract; Journal Article
  • [Publication-country] Poland
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57. Rösch C, Eilmus S, Bothe H: Approaches to assess the biodiversity of bacteria in natural habitats. Biochem Soc Trans; 2006 Feb;34(Pt 1):169-73
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  • Any attempt to characterize a bacterial community and their functional genes coding for enzymes of the nitrogen cycle is faced with its extreme biodiversity.
  • Computer-based assignment tools have now been developed utilizing terminal restriction fragments obtained from digestions with multiple restriction enzymes.
  • Such programs allow the gross characterization of bacterial life in any complex bacterial community with confidence.

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  • (PMID = 16417513.001).
  • [ISSN] 0300-5127
  • [Journal-full-title] Biochemical Society transactions
  • [ISO-abbreviation] Biochem. Soc. Trans.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Ribosomal, 16S
  • [Number-of-references] 37
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58. McCartt AT, Blackman K, Voas RB: Implementation of Washington State's zero tolerance law: patterns of arrests, dispositions, and recidivism. Traffic Inj Prev; 2007 Dec;8(4):339-45
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  • The present study examined effects of the ZT law on arrests and case dispositions among underage offenders as a function of blood alcohol concentration (BAC) and post-law patterns of recidivism.
  • RESULTS: There was a substantial increase in arrests of underage drivers beginning immediately after implementation of the ZT law, especially among drivers with low BACs.
  • Underage offenders with lower BACs became far more likely to receive alcohol-related convictions and/or license suspensions.
  • However, the percentage of underage offenders with higher BACs receiving DUI convictions declined as some of these offenders received the lesser ZT disposition.
  • After the ZT law, underage offenders with BACs of 0.10 g/dL or higher were more likely to recidivate than those with lower BACs, but appreciable proportions of drivers were re-arrested for another alcohol offense, whatever the BAC and however they were penalized.
  • However, recidivism remains an issue as more than one in four underage drivers arrested with low BACs subsequently were re-arrested.

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  • (PMID = 17994486.001).
  • [ISSN] 1538-9588
  • [Journal-full-title] Traffic injury prevention
  • [ISO-abbreviation] Traffic Inj Prev
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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59. Beaver LM, Stemmy EJ, Constant SL, Schwartz A, Little LG, Gigley JP, Chun G, Sugden KD, Ceryak SM, Patierno SR: Lung injury, inflammation and Akt signaling following inhalation of particulate hexavalent chromium. Toxicol Appl Pharmacol; 2009 Feb 15;235(1):47-56
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  • [Title] Lung injury, inflammation and Akt signaling following inhalation of particulate hexavalent chromium.
  • Certain particulate hexavalent chromium [Cr(VI)] compounds are human respiratory carcinogens that release genotoxic soluble chromate, and are associated with fibrosis, fibrosarcomas, adenocarcinomas and squamous cell carcinomas of the lung.
  • Our aim was to determine the nature of the lung injury, inflammatory response, and survival signaling responses following intranasal exposure of BALB/c mice to particulate basic zinc chromate.
  • Factors associated with lung injury, inflammation and survival signaling were measured in airway lavage fluid and in lung tissue.
  • A single chromate exposure induced an acute immune response in the lung, characterized by a rapid and significant increase in IL-6 and GRO-alpha levels, an influx of neutrophils, and a decline in macrophages in lung airways.
  • Histological examination of lung tissue in animals challenged with a single chromate exposure revealed an increase in bronchiolar cell apoptosis and mucosal injury.
  • Furthermore, chromate exposure induced injury and inflammation that progressed to alveolar and interstitial pneumonitis.
  • These data illustrate that chromate induces both survival signaling and an inflammatory response in the lung, which we postulate may contribute to early oncogenesis.

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  • (PMID = 19109987.001).
  • [ISSN] 1096-0333
  • [Journal-full-title] Toxicology and applied pharmacology
  • [ISO-abbreviation] Toxicol. Appl. Pharmacol.
  • [Language] ENG
  • [Grant] United States / NIEHS NIH HHS / ES / R01 ES009961; United States / NIAID NIH HHS / AI / R01-AI067254; United States / NIEHS NIH HHS / ES / R01-ES09961; United States / NCI NIH HHS / CA / R01 CA107972; United States / NIEHS NIH HHS / ES / R01-ES05304; United States / NIEHS NIH HHS / ES / R01 ES005304; United States / NCI NIH HHS / CA / R01-CA107972; United States / NIAID NIH HHS / AI / R01 AI067254
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Air Pollutants; 0 / Biomarkers, Tumor; 0R0008Q3JB / Chromium; 18540-29-9 / chromium hexavalent ion; EC 2.7.11.1 / Proto-Oncogene Proteins c-akt
  • [Other-IDs] NLM/ NIHMS458365; NLM/ PMC3640501
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60. Kim YK, Kwon JT, Choi JY, Jiang HL, Arote R, Jere D, Je YH, Cho MH, Cho CS: Suppression of tumor growth in xenograft model mice by programmed cell death 4 gene delivery using folate-PEG-baculovirus. Cancer Gene Ther; 2010 Nov;17(11):751-60
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  • [Title] Suppression of tumor growth in xenograft model mice by programmed cell death 4 gene delivery using folate-PEG-baculovirus.
  • Cancer gene therapy using tumor suppressor genes is considered to be an attractive approach for arresting cell growth and inducing apoptosis.
  • Programmed cell death 4 (Pdcd4) is a tumor suppressor gene, which prevents tumorigenesis and tumor progression.
  • Folate-PEG-baculovirus containing Pdcd4 gene (F-P-Bac-Pdcd4) was constructed by attachment of F-PEG to the baculovirus surface using chemical modification.
  • The F-P-Bac-Pdcd4 showed enhanced transduction efficiency, efficiently expressed PDCD4 protein, and induced apoptosis in human epidermal carcinoma (KB) cells as compared with an unmodified baculovirus.
  • In a tumor xenograft study, injection of F-P-Bac-Pdcd4 into tumors established from the KB cell line by subcutaneous implantation significantly suppressed tumor growth and induced apoptosis.
  • Thus, this study shows a new baculovirus-mediated tumor suppressor gene delivery system for cancer therapy.
  • [MeSH-major] Apoptosis Regulatory Proteins / metabolism. Baculoviridae / genetics. Carcinoma / therapy. Genes, Tumor Suppressor. RNA-Binding Proteins / metabolism. Transduction, Genetic
  • [MeSH-minor] Animals. Cell Line, Tumor. Folic Acid / analogs & derivatives. Folic Acid / metabolism. Gene Expression Regulation, Neoplastic. Genetic Therapy. Humans. Male. Mice. Mice, Inbred BALB C. Mice, Nude. Polyethylene Glycols. Xenograft Model Antitumor Assays

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  • (PMID = 20539318.001).
  • [ISSN] 1476-5500
  • [Journal-full-title] Cancer gene therapy
  • [ISO-abbreviation] Cancer Gene Ther.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Apoptosis Regulatory Proteins; 0 / Pdcd4 protein, mouse; 0 / RNA-Binding Proteins; 0 / poly(ethylene glycol)-folate; 30IQX730WE / Polyethylene Glycols; 935E97BOY8 / Folic Acid
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61. Juárez P, Walters ST, Daugherty M, Radi C: A randomized trial of motivational interviewing and feedback with heavy drinking college students. J Drug Educ; 2006;36(3):233-46
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  • At an eight-week follow-up, all groups reduced their consumption, peak BAC, consequences, and dependence symptoms.

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  • (PMID = 17345916.001).
  • [ISSN] 0047-2379
  • [Journal-full-title] Journal of drug education
  • [ISO-abbreviation] J Drug Educ
  • [Language] eng
  • [Grant] United States / NIAAA NIH HHS / AA / T32-AA07465
  • [Publication-type] Journal Article; Randomized Controlled Trial; Research Support, N.I.H., Extramural
  • [Publication-country] United States
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62. Safár J, Simková H, Kubaláková M, Cíhalíková J, Suchánková P, Bartos J, Dolezel J: Development of chromosome-specific BAC resources for genomics of bread wheat. Cytogenet Genome Res; 2010 Jul;129(1-3):211-23
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  • [Title] Development of chromosome-specific BAC resources for genomics of bread wheat.
  • Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries.
  • Each chromosome (arm)-specific library is composed of a manageable number of clones, and so represents a practical tool in the area of wheat genomics.
  • [MeSH-major] Chromosomes, Artificial, Bacterial / genetics. Chromosomes, Plant / genetics. Triticum / genetics

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  • [Copyright] Copyright 2010 S. Karger AG, Basel.
  • (PMID = 20501977.001).
  • [ISSN] 1424-859X
  • [Journal-full-title] Cytogenetic and genome research
  • [ISO-abbreviation] Cytogenet. Genome Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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63. Ahsan B, Kobayashi D, Yamada T, Kasahara M, Sasaki S, Saito TL, Nagayasu Y, Doi K, Nakatani Y, Qu W, Jindo T, Shimada A, Naruse K, Toyoda A, Kuroki Y, Fujiyama A, Sasaki T, Shimizu A, Asakawa S, Shimizu N, Hashimoto S, Yang J, Lee Y, Matsushima K, Sugano S, Sakaizumi M, Narita T, Ohishi K, Haga S, Ohta F, Nomoto H, Nogata K, Morishita T, Endo T, Shin-I T, Takeda H, Kohara Y, Morishita S: UTGB/medaka: genomic resource database for medaka biology. Nucleic Acids Res; 2008 Jan;36(Database issue):D747-52
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  • This database provides basic genomic information, such as predicted genes, expressed sequence tags (ESTs), guanine/cytosine (GC) content, repeats and comparative genomics, as well as unique data resources including (i) 2473 genetic markers and experimentally confirmed PCR primers that amplify these markers, (ii) 142,414 bacterial artificial chromosome (BAC) and 217,344 fosmid end sequences that amount to 15.0- and 11.1-fold clone coverage of the entire genome, respectively, and were used for draft genome assembly, (iii) 16,519,460 single nucleotide polymorphisms (SNPs), and 2 859 905 insertions/deletions detected between two medaka inbred strain genomes and (iv) 841 235 5'-end serial analyses of gene-expression (SAGE) tags that identified 344 266 transcription start sites on the genome.
  • [MeSH-minor] Animals. Chromosomes, Artificial, Bacterial. Gene Expression. Genetic Markers. Genetic Variation. Internet. Plasmids / genetics. Polymorphism, Single Nucleotide. Transcription Initiation Site. User-Computer Interface

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  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
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  • [Other-IDs] NLM/ PMC2238888
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64. Tischer BK, Kaufer BB, Sommer M, Wussow F, Arvin AM, Osterrieder N: A self-excisable infectious bacterial artificial chromosome clone of varicella-zoster virus allows analysis of the essential tegument protein encoded by ORF9. J Virol; 2007 Dec;81(23):13200-8
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  • [Title] A self-excisable infectious bacterial artificial chromosome clone of varicella-zoster virus allows analysis of the essential tegument protein encoded by ORF9.
  • In order to facilitate the generation of mutant viruses of varicella-zoster virus (VZV), the agent causing varicella (chicken pox) and herpes zoster (shingles), we generated a full-length infectious bacterial artificial chromosome (BAC) clone of the P-Oka strain.
  • Subsequently, mini-F-containing recombinant virus was generated from overlapping cosmid clones, and full-length VZV DNA recovered from the recombinant virus was established in Escherichia coli as an infectious BAC.

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  • (PMID = 17913822.001).
  • [ISSN] 1098-5514
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / R21 AI061412; United States / NIAID NIH HHS / AI / 1R21AI061412
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Viral; 0 / Viral Structural Proteins
  • [Other-IDs] NLM/ PMC2169085
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65. Yang Z, Zhang Y: [The application of nuclear medicine imaging in bronchioloalveolar carcinoma.]. Zhongguo Fei Ai Za Zhi; 2009 Oct 20;12(10):1123-6
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  • [Title] [The application of nuclear medicine imaging in bronchioloalveolar carcinoma.].

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  • (PMID = 20723354.001).
  • [ISSN] 1999-6187
  • [Journal-full-title] Zhongguo fei ai za zhi = Chinese journal of lung cancer
  • [ISO-abbreviation] Zhongguo Fei Ai Za Zhi
  • [Language] chi
  • [Publication-type] Journal Article
  • [Publication-country] China
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66. Tinianow JN, Gill HS, Ogasawara A, Flores JE, Vanderbilt AN, Luis E, Vandlen R, Darwish M, Junutula JR, Williams SP, Marik J: Site-specifically 89Zr-labeled monoclonal antibodies for ImmunoPET. Nucl Med Biol; 2010 Apr;37(3):289-97
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  • METHODS: The amino group of desferrioxamine B was acylated by bromoacetyl bromide, N-hydroxysuccinimidyl iodoacetate, or N-hydroxysuccinimidyl 4-[N-maleimidomethyl]cyclohexane-1-carboxylate to obtain thiol reactive reagents bromoacetyl-desferrioxamine (Df-Bac), iodoacetyl-desferrioxamine (Df-Iac) and maleimidocyclohexyl-desferrioxamine (Df-Chx-Mal), respectively.
  • Df-Bac and Df-Iac alkylated the free thiol groups of thio-trastuzumab by nucleophilic substitution forming Df-Ac-thio-trastuzumab, while the maleimide reagent Df-Chx-Mal reacted via Michael addition to provide Df-Chx-Mal-thio-trastuzumab.
  • RESULTS: The chemoselective reagents were obtained in 14% (Df-Bac), 53% (Df-Iac) and 45% (Df-Chx-Mal) yields.
  • Site-specific conjugation of Df-Chx-Mal to thio-trastuzumab was complete within 1 h at pH 7.5, while Df-Iac and Df-Bac respectively required 2 and 5 h at pH 9.
  • Each Df modified thio-trastuzumab was chelated with (89)Zr in yields exceeding 75%. (89)Zr-Df-Ac-thio-trastuzumab and (89)Zr-Df-Chx-Mal-thio-trastuzumab were stable in mouse serum and exhibited comparable PET imaging capabilities in a BT474M1 (HER2-positive) breast cancer model reaching 20-25 %ID/g of tumor uptake and a tumor to blood ratio of 6.1-7.1.

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  • [Copyright] Copyright 2010 Elsevier Inc. All rights reserved.
  • (PMID = 20346868.001).
  • [ISSN] 1872-9614
  • [Journal-full-title] Nuclear medicine and biology
  • [ISO-abbreviation] Nucl. Med. Biol.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / Radioisotopes; 0 / Radiopharmaceuticals; C6V6S92N3C / Zirconium
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67. Liu T, Mao D, Zhang S, Xu C, Xing Y: Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa). Theor Appl Genet; 2009 May;118(8):1509-17
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  • [Title] Fine mapping SPP1, a QTL controlling the number of spikelets per panicle, to a BAC clone in rice (Oryza sativa).
  • Finally, it was narrowed down to a bacterial artificial chromosome clone spanning 107 kb; 17 open reading frames have been identified in the region.
  • [MeSH-major] Chromosomes, Artificial, Bacterial. Genes, Plant. Oryza / genetics. Physical Chromosome Mapping. Quantitative Trait Loci

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  • (PMID = 19266175.001).
  • [ISSN] 1432-2242
  • [Journal-full-title] TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
  • [ISO-abbreviation] Theor. Appl. Genet.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / DNA, Plant; 0 / Genetic Markers
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68. Prior K, Hautefort I, Hinton JC, Richardson DJ, Rowley G: All stressed out. Salmonella pathogenesis and reactive nitrogen species. Adv Microb Physiol; 2009;56:1-28
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  • Bacterial pathogens must overcome a range of challenges during the process of infecting their host.
  • The ability of a pathogen to sense and respond appropriately to changes in host environment is vital if the pathogen is to succeed.
  • Mammalian defense strategies include the use of barriers like skin and epithelial surfaces, the production of a chemical arsenal, such as stomach acid and reactive oxygen and nitrogen species, and a highly coordinated cellular and humoral immune response.
  • Here we focus on the interplay which occurs between Salmonella and the host during the infection process, with particular emphasis on the complex bacterial response to reactive nitrogen species produced by the host.
  • We discuss recent advances in our understanding of the key mechanisms which confer bacterial resistance to nitrogen species, which in response to nitric oxide include the flavohemoglobin, HmpA, the flavorubredoxin, NorV, and the cytochrome c nitrite reductase, NrfA, whilst in response to nitrate include a repertoire of nitrate reductases.

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  • [Copyright] Copyright © 2009 Elsevier Ltd. All rights reserved.
  • (PMID = 20943123.001).
  • [ISSN] 2162-5468
  • [Journal-full-title] Advances in microbial physiology
  • [ISO-abbreviation] Adv. Microb. Physiol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Reactive Nitrogen Species; 31C4KY9ESH / Nitric Oxide; EC 1.7.- / Nitrite Reductases
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69. Sziksz E, Tibor Kozma G, Komlósi ZI, Pállinger E, Kardos M, Szebeni B, Losonczy G, Falus A, Szabó A, Tulassay T, Vannay A: Increased synthesis of vascular endothelial growth factor in allergic airway inflammation in histidine decarboxylase knockout (HDC(-/-)) mice. Exp Lung Res; 2010 Sep;36(7):420-30
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  • VEGF mRNA expression and protein level were determined in the lung.
  • Number of VEGF-positive immune cells of bronchoalveolar lavage (BAL) and their intracellular VEGF content were measured by flow cytometry.
  • VEGF protein level in the lung and in the BAL cells was increased in OVA treated (HDC(-/-)(ova) as well as in WT(ova)) animals compared to their controls.
  • However, there was no difference in the VEGF levels between HDC(-/-) or WT animals, either in the lung or in the BAL cells.
  • In conclusion, increased VEGF production of the lung or BAL immune cells can be induced by allergen provocation independently from the genetic background of the animals.
  • [MeSH-minor] Animals. Bronchoalveolar Lavage Fluid / cytology. Female. Lung / drug effects. Lung / enzymology. Lung / pathology. Mice. Mice, Knockout. Ovalbumin

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  • (PMID = 20715981.001).
  • [ISSN] 1521-0499
  • [Journal-full-title] Experimental lung research
  • [ISO-abbreviation] Exp. Lung Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Vascular Endothelial Growth Factors; 820484N8I3 / Histamine; 9006-59-1 / Ovalbumin; EC 4.1.1.22 / Histidine Decarboxylase
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70. Chen WY, Pulukkunat DK, Cho IM, Tsai HY, Gopalan V: Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complex. Nucleic Acids Res; 2010 Dec;38(22):8316-27
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  • [Title] Dissecting functional cooperation among protein subunits in archaeal RNase P, a catalytic ribonucleoprotein complex.
  • Biochemical studies on the bacterial holoenzyme, composed of one catalytic RNase P RNA (RPR) and one RNase P protein (RPP), have helped understand the pleiotropic roles (including substrate/Mg(2+) binding) by which a protein could facilitate RNA catalysis.
  • Exploiting our previous finding that these archaeal RPPs function as two binary RPP complexes (POP5•RPP30 and RPP21•RPP29), we prepared recombinant RPP pairs from three archaea and established interchangeability of subunits through homologous/heterologous assemblies.
  • Our finding that archaeal POP5•RPP30 reconstituted with bacterial and organellar RPRs suggests functional overlap of this binary complex with the bacterial RPP and highlights their shared recognition of a phylogenetically-conserved RPR catalytic core, whose minimal attributes we further defined through deletion mutagenesis.

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  • (PMID = 20705647.001).
  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM067807
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Archaeal Proteins; 0 / Protein Subunits; 0 / RNA Precursors; 9014-25-9 / RNA, Transfer; EC 3.1.26.5 / Ribonuclease P
  • [Other-IDs] NLM/ PMC3001054
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71. Bosnjak B, Ivetić Tkalcević V, Durić K, Belamarić D, Cuzić S, Ferencić Z, Brajsa K, Glojnarić I, Antolović R, Hrvacić B: Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma. Inflamm Res; 2009 Nov;58(11):773-81
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Intranasal challenge with increasing ovalbumin doses differently affects airway hyperresponsiveness and inflammatory cell accumulation in mouse model of asthma.
  • OBJECTIVE: To investigate whether challenge with increasing allergen doses could differently affect allergen-induced airway hyperresponsiveness (AHR) and inflammatory cell accumulation in mouse model of asthma, providing an experimental model to investigate their relationship.
  • MATERIAL AND METHODS: AHR and accumulation of inflammatory cells in bronchoalveolar lavage fluid (BALF) and into the lungs were compared in ovalbumin-sensitized mice that were challenged intranasally with 2.5, 10, 25 or 100 microg of ovalbumin/mouse.
  • RESULTS: Both AHR and inflammatory cell accumulation were proportional to the ovalbumin dose used for challenge.
  • [MeSH-minor] Animals. Bronchoalveolar Lavage Fluid / cytology. Bronchoalveolar Lavage Fluid / immunology. Cytokines / immunology. Disease Models, Animal. Eosinophil Peroxidase / metabolism. Eosinophils / immunology. Humans. Interleukin-13 / immunology. Lung / cytology. Lung / immunology. Lung / pathology. Male. Mice. Mice, Inbred BALB C

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  • (PMID = 19440656.001).
  • [ISSN] 1420-908X
  • [Journal-full-title] Inflammation research : official journal of the European Histamine Research Society ... [et al.]
  • [ISO-abbreviation] Inflamm. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / Cytokines; 0 / Interleukin-13; 9006-59-1 / Ovalbumin; EC 1.11.1.- / Eosinophil Peroxidase
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72. Sipos R, Székely AJ, Palatinszky M, Révész S, Márialigeti K, Nikolausz M: Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis. FEMS Microbiol Ecol; 2007 May;60(2):341-50
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  • [Title] Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis.
  • In the attempt to explore complex bacterial communities of environmental samples, primers hybridizing to phylogenetically highly conserved regions of 16S rRNA genes are widely used, but differential amplification is a recognized problem.
  • The distortion of the template-to-product ratio was measured using predefined template mixtures and environmental samples by terminal restriction fragment length polymorphism analysis.
  • As a result of additional tests on environmental samples, the use of a low annealing temperature is recommended in order to significantly reduce preferential amplification while maintaining the specificity of PCR.
  • [MeSH-minor] DNA, Bacterial / chemistry. DNA, Bacterial / genetics. Ecosystem. Electrophoresis, Capillary. Environmental Microbiology. Molecular Sequence Data. Polymorphism, Restriction Fragment Length. Sequence Analysis, DNA. Temperature

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  • (PMID = 17343679.001).
  • [ISSN] 0168-6496
  • [Journal-full-title] FEMS microbiology ecology
  • [ISO-abbreviation] FEMS Microbiol. Ecol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ DQ207728/ DQ207729/ DQ207730/ DQ207731
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA Primers; 0 / DNA, Bacterial; 0 / RNA, Ribosomal, 16S
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73. Portnoï D, Sertour N, Ferquel E, Garnier M, Baranton G, Postic D: A single-run, real-time PCR for detection and identification of Borrelia burgdorferi sensu lato species, based on the hbb gene sequence. FEMS Microbiol Lett; 2006 Jun;259(1):35-40
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  • Lyme borreliosis is the most important vector-borne disease caused by spirochetes within the Borrelia burgdorferi sensu lato (B. burgdorferi sl) complex.
  • There is strong evidence that different species of this group of genetically diverse spirochetes are involved in distinct clinical manifestations of the disease.
  • In order to differentiate species within this bacterial complex, we developed a real-time-PCR protocol, which targets the hbb gene.
  • We designed a fluorescein-labeled probe specific of a region of this gene harboring a polymorphism linked to species.
  • [MeSH-major] Bacterial Proteins / genetics. Borrelia burgdorferi Group / classification. Borrelia burgdorferi Group / isolation & purification. DNA-Binding Proteins / genetics. Polymerase Chain Reaction / methods
  • [MeSH-minor] Animals. Bacterial Typing Techniques. DNA Primers. DNA, Bacterial / analysis. DNA, Bacterial / isolation & purification. Reproducibility of Results. Sensitivity and Specificity. Ticks / microbiology. Transition Temperature

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  • (PMID = 16684099.001).
  • [ISSN] 0378-1097
  • [Journal-full-title] FEMS microbiology letters
  • [ISO-abbreviation] FEMS Microbiol. Lett.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / DNA Primers; 0 / DNA, Bacterial; 0 / DNA-Binding Proteins; 0 / Hbb protein, Borrelia burgdorferi
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74. Macfarlane S, Woodmansey EJ, Macfarlane GT: Colonization of mucin by human intestinal bacteria and establishment of biofilm communities in a two-stage continuous culture system. Appl Environ Microbiol; 2005 Nov;71(11):7483-92
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  • The human large intestine is covered with a protective mucus coating, which is heavily colonized by complex bacterial populations that are distinct from those in the gut lumen.
  • The establishment of bacterial communities in mucin gels was investigated by selective culture methods, scanning electron microscopy, and confocal laser scanning microscopy, in association with fluorescently labeled 16S rRNA oligonucleotide probes.
  • Mucin gels were rapidly colonized by heterogeneous bacterial populations, especially members of the Bacteroides fragilis group, enterobacteria, and clostridia.
  • Intestinal bacterial populations growing on mucin surfaces were shown to be phylogenetically and metabolically distinct from their planktonic counterparts.

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  • (PMID = 16269790.001).
  • [ISSN] 0099-2240
  • [Journal-full-title] Applied and environmental microbiology
  • [ISO-abbreviation] Appl. Environ. Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Culture Media; 0 / Fluorescent Dyes; 0 / Mucins; 0 / Oligonucleotide Probes; 0 / RNA, Ribosomal, 16S
  • [Other-IDs] NLM/ PMC1287682
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75. Gearhardt AN, Corbin WR: Body mass index and alcohol consumption: family history of alcoholism as a moderator. Psychol Addict Behav; 2009 Jun;23(2):216-25
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  • BMI, family history of alcoholism, gender, and race/ethnicity were assessed as predictors of typical drinking frequency and estimated blood alcohol concentration (BAC).

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  • [Copyright] Copyright (c) 2009 APA, all rights reserved.
  • (PMID = 19586138.001).
  • [ISSN] 0893-164X
  • [Journal-full-title] Psychology of addictive behaviors : journal of the Society of Psychologists in Addictive Behaviors
  • [ISO-abbreviation] Psychol Addict Behav
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] United States
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76. Misfeldt AM, Boyle SC, Tompkins KL, Bautch VL, Labosky PA, Baldwin HS: Endocardial cells are a distinct endothelial lineage derived from Flk1+ multipotent cardiovascular progenitors. Dev Biol; 2009 Sep 01;333(1):78-89
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  • To distinguish endocardium from other vasculature, we generated an NFATc1-nuc-LacZ BAC transgenic mouse line capable of labeling this specific endothelial subpopulation at the earliest stages of cardiac development.
  • Endocardium is specified as a cardiac cell lineage, independent from other vascular populations, responding to BMP and Wnt signals that enhance cardiomyocyte differentiation.
  • [MeSH-major] Cell Lineage / physiology. Embryonic Stem Cells / physiology. Endocardium / embryology. Endothelial Cells / physiology. Multipotent Stem Cells / physiology. Myocytes, Cardiac / physiology. Vascular Endothelial Growth Factor Receptor-2 / metabolism
  • [MeSH-minor] Animals. Antigens, Differentiation / metabolism. Cell Differentiation / physiology. Cells, Cultured. Hematopoietic Stem Cells / cytology. Hematopoietic Stem Cells / physiology. Mesoderm / cytology. Mesoderm / embryology. Mice. Mice, Transgenic. Muscle, Smooth, Vascular / cytology. Muscle, Smooth, Vascular / embryology. Myocytes, Smooth Muscle / cytology. Myocytes, Smooth Muscle / physiology. NFATC Transcription Factors / genetics

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  • (PMID = 19576203.001).
  • [ISSN] 1095-564X
  • [Journal-full-title] Developmental biology
  • [ISO-abbreviation] Dev. Biol.
  • [Language] eng
  • [Grant] United States / NHLBI NIH HHS / HL / RL1 HL092551; United States / NHLBI NIH HHS / HL / RL1HL0952551-01; United States / NIGMS NIH HHS / GM / T32 GM07347
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Differentiation; 0 / NFATC Transcription Factors; 0 / Nfatc1 protein, mouse; EC 2.7.10.1 / Vascular Endothelial Growth Factor Receptor-2
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77. Shi JJ, Cai DH, Chen XJ, Sheng HZ: Cloning and characterization of the rabbit POU5F1 gene. DNA Seq; 2008 Feb;19(1):56-61
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  • The rabbit POUSF1 gene was mapped to chromosome 12q1.1 by PCR amplification of DNA from two putative POU5F1-containing BAC clones, which were previously mapped to chromosome 12q1.1.

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  • (PMID = 17852331.001).
  • [ISSN] 1042-5179
  • [Journal-full-title] DNA sequence : the journal of DNA sequencing and mapping
  • [ISO-abbreviation] DNA Seq.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ EF062856/ EF194086
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Octamer Transcription Factor-3
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78. Titus TA, Selvig DR, Qin B, Wilson C, Starks AM, Roe BA, Postlethwait JH: The Fanconi anemia gene network is conserved from zebrafish to human. Gene; 2006 Apr 26;371(2):211-23
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  • Fanconi anemia (FA) is a complex disease involving nine identified and two unidentified loci that define a network essential for maintaining genomic stability.
  • To test the hypothesis that the FA network is conserved in vertebrate genomes, we cloned and sequenced zebrafish (Danio rerio) cDNAs and/or genomic BAC clones orthologous to all nine cloned FA genes (FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, and FANCL), and identified orthologs in the genome database for the pufferfish Tetraodon nigroviridis.
  • Evolutionarily conserved regions identified functionally important domains, since many amino acid residues mutated in human disease alleles or shown to be critical in targeted mutagenesis studies are identical in zebrafish and human.
  • [MeSH-minor] Amino Acid Sequence. Animals. Chromosomes, Artificial, Bacterial. Humans. Molecular Sequence Data. Sequence Homology, Amino Acid

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  • (PMID = 16515849.001).
  • [ISSN] 0378-1119
  • [Journal-full-title] Gene
  • [ISO-abbreviation] Gene
  • [Language] eng
  • [Grant] United States / NICHD NIH HHS / HD / HD22486; United States / NCRR NIH HHS / RR / R01RR10715
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Fanconi Anemia Complementation Group D2 Protein
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79. Moore S, Shepherd J, Perham N, Cusens B: The prevalence of alcohol intoxication in the night-time economy. Alcohol Alcohol; 2007 Nov-Dec;42(6):629-34
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  • METHOD: A random sample of 893 people were interviewed and breathalysed in 24 repeated, cross-sectional surveys over the course of a year in the city centre streets of a European capital city between 11.00 pm and 3.00 am.
  • RESULTS: Median blood alcohol concentration (BAC) in men was 0.13% (min = 0%, max = 0.33%) and in women was 0.09% (min = 0%, max = 0.27%)-which were below the threshold used to indicate 'at risk BAC' (0.15%; for men t = 9.32, P < 0.001 and for women t = 17.54, P < 0.001).
  • Men provided higher BACs than women (t = 7.17, P < 0.001).
  • The relationship between age and BAC for men described an inverted 'u', peaking at 29 years, but for women the relationship was positive and linear.
  • BAC was inversely related to the ability to remember and report the evening's consumption (z = 4.76, P < 0.001).
  • Reported consumption predicted only 12% (P < 0.001) of the variance in BAC for men and 10% (P < 0.001) for women.
  • The probability for respondents to recall past consumption diminished as BAC increased suggesting self-report data are not suitable to assess consumption in heavy drinkers.

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  • (PMID = 17660522.001).
  • [ISSN] 1464-3502
  • [Journal-full-title] Alcohol and alcoholism (Oxford, Oxfordshire)
  • [ISO-abbreviation] Alcohol Alcohol.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
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80. Philipp U, Quignon P, Scott A, André C, Breen M, Leeb T: Chromosomal assignment of the canine melanophilin gene (MLPH): a candidate gene for coat color dilution in Pinschers. J Hered; 2005;96(7):774-6
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  • To investigate the genetic cause of the coat color dilution in Pinschers, we isolated BAC clones containing the canine ortholog of the known murine color dilution gene Mlph.
  • RH mapping of the canine MLPH gene was performed using an STS marker derived from BAC sequences.
  • Additionally, one MLPH BAC clone was used as probe for FISH mapping, and the canine MLPH gene was assigned to CFA25q24.

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  • (PMID = 15958794.001).
  • [ISSN] 0022-1503
  • [Journal-full-title] The Journal of heredity
  • [ISO-abbreviation] J. Hered.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Proteins
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81. Wang YJ, Jiang YL, Tang HF, Zhao CZ, Chen JQ: Zl-n-91, a selective phosphodiesterase 4 inhibitor, suppresses inflammatory response in a COPD-like rat model. Int Immunopharmacol; 2010 Feb;10(2):252-8
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  • Chronic obstructive pulmonary disease (COPD) is defined as a disease state characterized by poorly reversible airflow limitation induced by cigarette smoking and/or other noxious particle and gases.
  • Phosphodiesterase (PDE) 4 inhibitors are known to elevated cAMP concentrations in inflammatory cells, leading to inhibition of inflammatory response, relaxation of smooth muscle in the airway, and modulation of sensory nerves in the lung as well.
  • To investigate whether Zl-n-91, a new selective PDE4 inhibitor, could decrease inflammation and improve lung function in a COPD-like rat model, male Sprague-Dawley rats are used to challenge with lipopolysaccharide (LPS) and cigarette smoking (CS) exposure to induce COPD-like animal model.
  • Administration of Zl-n-91 at different dosages results in decreases of inflammatory cell in bronchoalveolar lavage fluid (BALF) as compared with vehicle treatment.
  • [MeSH-major] Anti-Inflammatory Agents, Non-Steroidal / therapeutic use. Furans / therapeutic use. Phenyl Ethers / therapeutic use. Phosphodiesterase 4 Inhibitors. Phosphodiesterase Inhibitors / therapeutic use. Pulmonary Disease, Chronic Obstructive / drug therapy
  • [MeSH-minor] Animals. Cyclic Nucleotide Phosphodiesterases, Type 4 / immunology. Disease Models, Animal. Lipopolysaccharides / immunology. Lung / drug effects. Lung / immunology. Lung / physiopathology. Male. Matrix Metalloproteinase 9 / immunology. Matrix Metalloproteinase Inhibitors. Neutrophils / drug effects. Neutrophils / immunology. Peroxidase / drug effects. Peroxidase / immunology. Rats. Rats, Sprague-Dawley. Smoking / adverse effects. Smoking / immunology

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  • [Copyright] Copyright 2009 Elsevier B.V. All rights reserved.
  • (PMID = 19914404.001).
  • [ISSN] 1878-1705
  • [Journal-full-title] International immunopharmacology
  • [ISO-abbreviation] Int. Immunopharmacol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Anti-Inflammatory Agents, Non-Steroidal; 0 / Furans; 0 / Lipopolysaccharides; 0 / Matrix Metalloproteinase Inhibitors; 0 / Phenyl Ethers; 0 / Phosphodiesterase 4 Inhibitors; 0 / Phosphodiesterase Inhibitors; 0 / Zl-n-91 compound; EC 1.11.1.7 / Peroxidase; EC 3.1.4.17 / Cyclic Nucleotide Phosphodiesterases, Type 4; EC 3.4.24.35 / Matrix Metalloproteinase 9
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82. Ludovini V, Gori S, Pistola L, Della Fazia MA, Piobbico D, Servillo G, Flacco A, Scuderi C, Crinò L: Long-lasting complete remission with tyrosine kinase inhibitor in bronchioloalveolar carcinoma with a so far unknown EGFR mutation. J Thorac Oncol; 2008 Apr;3(4):452-3
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  • [Title] Long-lasting complete remission with tyrosine kinase inhibitor in bronchioloalveolar carcinoma with a so far unknown EGFR mutation.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / drug therapy. Adenocarcinoma, Bronchiolo-Alveolar / genetics. Antineoplastic Combined Chemotherapy Protocols / therapeutic use. Lung Neoplasms / drug therapy. Lung Neoplasms / genetics. Mutation / genetics. Protein-Tyrosine Kinases / antagonists & inhibitors. Receptor, Epidermal Growth Factor / genetics

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  • (PMID = 18379371.001).
  • [ISSN] 1556-1380
  • [Journal-full-title] Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer
  • [ISO-abbreviation] J Thorac Oncol
  • [Language] eng
  • [Publication-type] Case Reports; Letter; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0W860991D6 / Deoxycytidine; B76N6SBZ8R / gemcitabine; EC 2.7.10.1 / Protein-Tyrosine Kinases; EC 2.7.10.1 / Receptor, Epidermal Growth Factor; Q20Q21Q62J / Cisplatin
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83. Cupp JS, Wrede JE, Cherry AM, Arber DA, George TI: Chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type and bronchogenic adenocarcinoma. Appl Immunohistochem Mol Morphol; 2008 May;16(3):296-300
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  • [Title] Chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue type and bronchogenic adenocarcinoma.
  • We present the chromosomal aberrations in a case of synchronous extranodal marginal zone B-cell lymphoma and bronchogenic adenocarcinoma with bronchioloalveolar features.

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  • (PMID = 18301237.001).
  • [ISSN] 1533-4058
  • [Journal-full-title] Applied immunohistochemistry & molecular morphology : AIMM
  • [ISO-abbreviation] Appl. Immunohistochem. Mol. Morphol.
  • [Language] ENG
  • [Publication-type] Case Reports; Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Immunoglobulin Heavy Chains; 0 / Neoplasm Proteins; EC 3.4.22.- / Caspases; EC 3.4.22.- / MALT1 protein, human
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84. Tischer BK, Smith GA, Osterrieder N: En passant mutagenesis: a two step markerless red recombination system. Methods Mol Biol; 2010;634:421-30
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  • Bacterial artificial chromosomes are used to maintain and modify large sequences of different origins in Escherichia coli.
  • Since foreign sequences, such as antibiotic resistance genes as well as frt- or loxP-sites are often unwanted in mutant BAC clones, we developed a Red-based technique that allows for the scarless generation of point mutations, deletions, and insertion of smaller and larger sequences.
  • [MeSH-minor] Chromosomes, Artificial, Bacterial

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  • (PMID = 20677001.001).
  • [ISSN] 1940-6029
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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85. Xian HQ, Werth K, Gottlieb DI: Promoter analysis in ES cell-derived neural cells. Biochem Biophys Res Commun; 2005 Feb 4;327(1):155-62
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  • [Title] Promoter analysis in ES cell-derived neural cells.
  • Neural cells derived from ES cells in cell culture (ESNCs) have many of the properties of normal neural cells and provide a model of "neurogenesis-in-a-dish."
  • ES cells are transfected with bacterial artificial chromosomes (BACs) containing Olig2, a gene with a key role in neural fate choice.
  • One BAC is modified by recombineering to insert a reporter gene and a gene for selecting stably transfected clones.
  • Another BAC contains a deletion of a suspected Olig2 promoter.
  • The combination of ESNCs and BAC recombineering will have broad application for analyzing gene transcription in the nervous system and will be applicable to human ES cells.
  • The general approach should also be applicable to the many other cell lineages that can now be derived from mouse and human ES cells in culture.
  • [MeSH-minor] Animals. Basic Helix-Loop-Helix Transcription Factors. Cell Differentiation. Cell Line. Chromosomes, Artificial, Bacterial / genetics. Gene Expression. Mice. Nerve Tissue Proteins / genetics. Phenotype. Sequence Deletion / genetics. Transgenes / genetics. Up-Regulation / genetics

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  • (PMID = 15629444.001).
  • [ISSN] 0006-291X
  • [Journal-full-title] Biochemical and biophysical research communications
  • [ISO-abbreviation] Biochem. Biophys. Res. Commun.
  • [Language] eng
  • [Grant] United States / NINDS NIH HHS / NS / NS045809; United States / NINDS NIH HHS / NS / P01 NS39577-03
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Basic Helix-Loop-Helix Transcription Factors; 0 / Nerve Tissue Proteins; 0 / Olig2 protein, mouse
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86. Quoix E: [Recent development of the standards of treatment of lung cancer]. Rev Prat; 2007 Feb 15;57(3):239-45
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  • [Title] [Recent development of the standards of treatment of lung cancer].
  • [Transliterated title] Evolutions récentes des traitements de référence du cancer bronchique primitif.
  • Non-small cell lung cancer (NSCLC) knows important changes with the development of the use of chemotherapy not only in the advanced forms but also in a perioperative setting.
  • In early stage disease, a wedge resection seems to be sufficient in the tumours with a diameter less than 2 cm and presenting as ground-glass opacity.
  • Targeted therapies have been the real novelty in the treatment of non-small cell lung cancer.
  • However, they have proved useful in second line setting and in first line setting in diffuse bronchioloalveolar carcinoma.
  • Regarding small-cell lung cancer, there has been no real novelty, the standard treatment remains unchanged.
  • [MeSH-major] Carcinoma, Non-Small-Cell Lung / surgery. Lung Neoplasms / surgery. Neoadjuvant Therapy
  • [MeSH-minor] Adenocarcinoma, Bronchiolo-Alveolar / surgery. Carcinoma, Small Cell / surgery. Chemotherapy, Adjuvant. Humans. Pneumonectomy. Radiotherapy, Adjuvant


87. Shao CW, Chen SL, Scheuring CF, Xu JY, Sha ZX, Dong XL, Zhang HB: Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes. Mar Biotechnol (NY); 2010 Oct;12(5):558-68
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  • [Title] Construction of two BAC libraries from half-smooth tongue sole Cynoglossus semilaevis and identification of clones containing candidate sex-determination genes.
  • Two bacterial artificial chromosome (BAC) libraries for C. semilaevis, with large, high-quality inserts and deep coverage, were constructed in the BamHI and HindIII sites of the vector pECBAC1.
  • The two libraries contain a total of 55,296 BAC clones arrayed in 144 384-well microtiter plates and correspond to 13.36 haploid genome equivalents.
  • Thus, the two BAC libraries of C. semilaevis provided a readily useable platform for genomics research, illustrated by the isolation of sex determination gene(s).
  • [MeSH-major] Chromosomes, Artificial, Bacterial / genetics. Cloning, Molecular. Fisheries / methods. Flatfishes / genetics. Gene Expression Regulation / genetics. Gene Library. Sex Determination Processes / genetics

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  • (PMID = 19957095.001).
  • [ISSN] 1436-2236
  • [Journal-full-title] Marine biotechnology (New York, N.Y.)
  • [ISO-abbreviation] Mar. Biotechnol.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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88. Khiabani HZ, Opdal MS, Mørland J: Blood alcohol concentrations in apprehended drivers of cars and boats suspected to be impaired by the police. Traffic Inj Prev; 2008 Mar;9(1):31-6
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • OBJECTIVE: According to the Norwegian Road Traffic Act, car drivers are not allowed to operate a vehicle with a blood alcohol concentration (BAC) above 0.2 g/kg.
  • Depending on the size of the boat or ship, boat drivers/captains/first mates are not allowed to conduct the boat with a BAC above 0.8 g/kg when driving small boats (length less than 15 m) and above 1.5 g/kg when running larger vessels/ships.
  • The new Sea Act of June 2005 states that captains/first mates cannot conduct a ship if he/she has a BAC above 0.2 g/kg.
  • Our aim was to determine the current median BAC in a large population of car and boat drivers in Norway.
  • Our other aim was to study if median BAC was higher in boat drivers than in car drivers who were suspected by the police to be impaired.
  • Furthermore, we wanted to investigate if the BAC levels were differently distributed by gender or age within and between these two groups.
  • The median BAC in boat drivers (1.76 g/kg [range 0.02-3.54]) was significantly higher compared to that in car drivers (1.54 g/kg [range 0.00-4.27]).
  • In the car driver group, the mean BAC did not differ significantly between men and women.
  • The median level of BAC was significantly higher in men than in women in the boat driver group (1.77 g/kg with CI 1.69-1.85 vs. 1.27 g/kg with CI 0.78-1.76).
  • The median BAC in apprehended boat drivers was considerably high in the present study.
  • The median BAC was also high in car drivers despite strict legislation.

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  • (PMID = 18338292.001).
  • [ISSN] 1538-957X
  • [Journal-full-title] Traffic injury prevention
  • [ISO-abbreviation] Traffic Inj Prev
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 3K9958V90M / Ethanol
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89. Boyadjiev SA, South ST, Radford CL, Patel A, Zhang G, Hur DJ, Thomas GH, Gearhart JP, Stetten G: A reciprocal translocation 46,XY,t(8;9)(p11.2;q13) in a bladder exstrophy patient disrupts CNTNAP3 and presents evidence of a pericentromeric duplication on chromosome 9. Genomics; 2005 May;85(5):622-9
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  • [Title] A reciprocal translocation 46,XY,t(8;9)(p11.2;q13) in a bladder exstrophy patient disrupts CNTNAP3 and presents evidence of a pericentromeric duplication on chromosome 9.
  • A patient with sporadic bladder exstrophy and de novo apparently balanced chromosomal translocation 46,XY,t(8;9)(p11.2;q13) was analyzed by fluorescence in situ hybridization (FISH) and molecular methods.
  • The chromosome 8p11.2 breakpoint was mapped to BAC clone RP4-547J18, predicted to contain several hypothetical genes.
  • Characterization of the chromosome 9q13 breakpoint indicated a disruption in the 5' region of CNTNAP3 within BAC RP11-292B8.
  • The identification of multiple copies of three BAC clones in the proband, his parents, and unrelated controls suggests that duplications of CNTNAP3 and the surrounding genomic region have occurred as a result of repeated events of unequal crossing over and pericentric inversions during chromosome 9 evolution.
  • [MeSH-minor] Base Sequence. Blotting, Northern. Blotting, Southern. Chromosome Mapping. Chromosomes, Artificial, Bacterial. DNA Primers. Gene Duplication. Humans. In Situ Hybridization, Fluorescence. Karyotyping. Male. Molecular Sequence Data. Reverse Transcriptase Polymerase Chain Reaction. Sequence Analysis, DNA


90. Koski A, Vuori E, Ojanperä I: Newer antidepressants: evaluation of fatal toxicity index and interaction with alcohol based on Finnish postmortem data. Int J Legal Med; 2005 Nov;119(6):344-8
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  • In fatal poisonings involving alcohol in combination with venlafaxine, mianserin, moclobemide, or mirtazapine, the median blood alcohol concentration (BAC) ranged from 2.35 to 2.7 mg/g, whereas in those involving alcohol in combination with citalopram or fluoxetine the median BAC was 2.9 and 3.4 mg/g, respectively.
  • The BAC was significantly lower in venlafaxine-related deaths than in those involving fluoxetine or citalopram.

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  • (PMID = 15739105.001).
  • [ISSN] 0937-9827
  • [Journal-full-title] International journal of legal medicine
  • [ISO-abbreviation] Int. J. Legal Med.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antidepressive Agents; 0 / Central Nervous System Depressants; 0 / Serotonin Uptake Inhibitors; 3K9958V90M / Ethanol
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91. Scagliotti GV, Smit E, Bosquee L, O'Brien M, Ardizzoni A, Zatloukal P, Eberhardt W, Smid-Geirnaerdt M, de Bruin HG, Dussenne S, Legrand C, Giaccone G, European Organisation for Research and Treatment of Cancer (EORTC) Lung Cancer Group (LCG): A phase II study of paclitaxel in advanced bronchioloalveolar carcinoma (EORTC trial 08956). Lung Cancer; 2005 Oct;50(1):91-6
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  • [Title] A phase II study of paclitaxel in advanced bronchioloalveolar carcinoma (EORTC trial 08956).
  • PURPOSE: The incidence of bronchioloalveolar carcinoma (BAC) has risen steadily over the last decades along with the increasing frequency of adenocarcinomas.
  • BAC is relatively resistant to commonly used chemotherapy regimens.
  • A phase II study with single agent paclitaxel in patients with stages IIIB, IV or recurrent BAC was performed.
  • EXPERIMENTAL DESIGN: Patients with BAC with at least one target bidimensionally measurable lesion staged as unresectable stages IIIB, IV or recurrent disease, not previously irradiated; ECOG performance status 0-2; life expectancy greater than 3 months; age range between 18 and 75, received paclitaxel at a dose of 200 mg/m2 i.v. as 3h continuous infusion on day 1 every 21 days.
  • After an independent review, two PR were confirmed, for a response rate of 11.1% (95% CI: 1.4-34.7%); nine patients had stable disease (50.0%), three patients had progressive disease (11.1%) and four patients were not assessable (22.2%).
  • CONCLUSIONS: Paclitaxel as single agent in stages IIIB-IV BAC was well tolerated and manageable but of limited efficacy.
  • BAC should not be excluded from trials of new forms of chemotherapy.
  • [MeSH-major] Adenocarcinoma, Bronchiolo-Alveolar / drug therapy. Antineoplastic Agents, Phytogenic / therapeutic use. Carcinoma, Non-Small-Cell Lung / drug therapy. Lung Neoplasms / drug therapy. Paclitaxel / therapeutic use
  • [MeSH-minor] Adult. Aged. Disease Progression. Female. Humans. Infusions, Intravenous. Male. Middle Aged. Survival Analysis. Treatment Outcome

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  • (PMID = 16019107.001).
  • [ISSN] 0169-5002
  • [Journal-full-title] Lung cancer (Amsterdam, Netherlands)
  • [ISO-abbreviation] Lung Cancer
  • [Language] eng
  • [Publication-type] Clinical Trial, Phase II; Journal Article; Multicenter Study
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Antineoplastic Agents, Phytogenic; P88XT4IS4D / Paclitaxel
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92. Knopf CW, Zavidij O, Rezuchova I, Rajcáni J: Evaluation of the T-REx transcription switch for conditional expression and regulation of HSV-1 vectors. Virus Genes; 2008 Feb;36(1):55-66
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  • Herpes simplex virus 1 (HSV-1) strain ANG and ANGpath were cloned as bacterial artificial chromosome (BAC).
  • Two different types of BAC genomes were obtained.
  • BAC genomes of type I contained the BAC replicon at the intended target region between the genes of UL48 and UL49.
  • In BAC genomes of type II, the BAC sequences were found to be aberrantly fused between the termini of the HSV-1 genome.
  • Both the BAC types were used to establish a conditional gene expression system for HSV-1 by Flp recombinase-mediated insertion of expression vectors that were modified to respond to the T-REx tetracycline (Tet)-inducible transcription switch.
  • During BAC cloning and mutagenesis in E. coli, not only deletions but also defined mutations of the HSV-1 genome were observed.
  • Successful virus reconstitution from BACs with large inserts demonstrated that HSV-1 has a packaging capacity for foreign sequences of at least 8.1% of its genome size.
  • In contrast, an efficient Tet-response was achieved with the gBGFP reporter, which was optimal in a Tet repressor (TetR)-producing cell line, demonstrating that the TetR concentration provided by the virus was not sufficient for a tight control of Tet-regulated gene expression.
  • [MeSH-major] Chromosomes, Artificial, Bacterial / genetics. Gene Expression Regulation, Viral. Genetic Vectors. Herpesvirus 1, Human / genetics. Transcription, Genetic
  • [MeSH-minor] Cell Line. Cloning, Molecular. Genes, Reporter. Green Fluorescent Proteins / genetics. Humans. Mutation. Plasmids / genetics. Recombinant Fusion Proteins / metabolism. Tetracyclines / pharmacology. Transfection. Viral Proteins / genetics

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  • (PMID = 18193347.001).
  • [ISSN] 0920-8569
  • [Journal-full-title] Virus genes
  • [ISO-abbreviation] Virus Genes
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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93. Han Y, Korban SS: An overview of the apple genome through BAC end sequence analysis. Plant Mol Biol; 2008 Aug;67(6):581-8
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  • [Title] An overview of the apple genome through BAC end sequence analysis.
  • A bacterial artificial chromosome (BAC)-based physical map of the apple genome has been recently constructed.
  • Based on this physical map, a total of approximately 2,100 clones from different contigs (overlapping BAC clones) have been selected and sequenced at both ends, generating 3,744 high-quality BAC end sequences (BESs) including 1,717 BAC end pairs.
  • A small number of BES pairs were mapped to neighboring chromosome regions of A. thaliana and Populus trichocarpa; whereas, no pairs are mapped to the Oryza sativa genome.
  • BAC end sequencing can be used to anchor a small proportion of the apple genome to the Populus and possibly to the Arabidopsis genomes.
  • [MeSH-major] Chromosomes, Artificial, Bacterial. Genome, Plant. Malus / genetics

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  • (PMID = 18521706.001).
  • [ISSN] 0167-4412
  • [Journal-full-title] Plant molecular biology
  • [ISO-abbreviation] Plant Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA Transposable Elements; 0 / Plant Proteins; 0 / Retroelements
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94. Skulan AJ, Nielsen IM, Melius CF, Allendorf MD: BAC-MP4 predictions of thermochemistry for gas-phase indium compounds in the In-H-C-O-Cl system. J Phys Chem A; 2006 Jan 12;110(1):281-90
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  • [Title] BAC-MP4 predictions of thermochemistry for gas-phase indium compounds in the In-H-C-O-Cl system.
  • The BAC-MP4 methodology has been used to calculate the heats of formation and associated thermodynamic parameters for a family of 51 different one-, two-, and three-coordinate indium compounds.
  • The BAC-MP4 methodology was calibrated by both experimental data and high-level coupled-cluster calculations.

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  • (PMID = 16392866.001).
  • [ISSN] 1089-5639
  • [Journal-full-title] The journal of physical chemistry. A
  • [ISO-abbreviation] J Phys Chem A
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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95. Leont'eva GF, Suvorov AN, Meringova LF, Grabovskaia KB, Ustinovich IA, Totolian AA: [Experimental evaluation of recombinant polypeptides as Streptococcus Group B vaccine]. Zh Mikrobiol Epidemiol Immunobiol; 2005 Mar-Apr;(2):35-40
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  • Recombinant polypeptides corresponding to the conservative N-terminal area of Bac surface protein of group B streptococci (GBS) and having the human IgA binding-site were obtained and evaluated in terms of Immunogenic and protective properties.
  • Polypeptide P6 had a molecular weight of 35 kD, The fragment of recombinant Bac protein with a molecular weight of 35 kD was found to have protective properties: when used for the subcutaneous immunization of animals, it stimulated the development of immune response capable, in case of challenge, to induce accelerated elimination of streptococci and the prevention of the mice death.
  • Recombinant fragment P6 of GBS protein Bac may be regarded as a candidate for inclusion into GBS-specific vaccine.
  • [MeSH-minor] Animals. Antibodies, Bacterial / biosynthesis. Antibodies, Bacterial / blood. Disease Models, Animal. Immunoglobulin A / biosynthesis. Injections, Subcutaneous. Male. Mice. Molecular Weight. Peptides / administration & dosage. Peptides / chemistry. Peptides / immunology. Recombinant Proteins / administration & dosage. Recombinant Proteins / chemistry. Recombinant Proteins / immunology. Species Specificity. Vaccines, Synthetic / administration & dosage. Vaccines, Synthetic / chemistry. Vaccines, Synthetic / immunology

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  • (PMID = 15881936.001).
  • [ISSN] 0372-9311
  • [Journal-full-title] Zhurnal mikrobiologii, epidemiologii, i immunobiologii
  • [ISO-abbreviation] Zh. Mikrobiol. Epidemiol. Immunobiol.
  • [Language] rus
  • [Publication-type] Comparative Study; English Abstract; Journal Article
  • [Publication-country] Russia (Federation)
  • [Chemical-registry-number] 0 / Antibodies, Bacterial; 0 / Immunoglobulin A; 0 / Peptides; 0 / Recombinant Proteins; 0 / Streptococcal Vaccines; 0 / Vaccines, Synthetic
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96. St-Jean JR, Desforges M, Almazán F, Jacomy H, Enjuanes L, Talbot PJ: Recovery of a neurovirulent human coronavirus OC43 from an infectious cDNA clone. J Virol; 2006 Apr;80(7):3670-4
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  • [Title] Recovery of a neurovirulent human coronavirus OC43 from an infectious cDNA clone.
  • This study describes the assembly of a full-length cDNA clone of human coronavirus (HCoV)-OC43 in a bacterial artificial chromosome (BAC).
  • The BAC containing the full-length infectious cDNA (pBAC-OC43(FL)) was assembled using a two-part strategy.
  • The first step consisted in the introduction of each end of the viral genome into the BAC with accessory sequences allowing proper transcription.
  • The second step consisted in the insertion of the whole HCoV-OC43 cDNA genome into the BAC.
  • Recombinant virus displayed the same phenotypic properties as the wild-type virus, including infectious virus titers produced in cell culture and neurovirulence in mice.
  • [MeSH-minor] Animals. Cell Line. Chromosomes, Artificial, Bacterial. Clone Cells. Coronavirus Infections / physiopathology. Coronavirus Infections / virology. Cricetinae. Genome, Viral. Humans. Injections, Intraventricular. Kinetics. Mice. Mice, Inbred BALB C. Mice, Inbred C57BL. Recombination, Genetic. Survival Rate. Transfection. Virion. Virulence. Virus Assembly. Virus Replication

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  • (PMID = 16537637.001).
  • [ISSN] 0022-538X
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Complementary
  • [Other-IDs] NLM/ PMC1440365
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97. DeLaurier A, Eames BF, Blanco-Sánchez B, Peng G, He X, Swartz ME, Ullmann B, Westerfield M, Kimmel CB: Zebrafish sp7:EGFP: a transgenic for studying otic vesicle formation, skeletogenesis, and bone regeneration. Genesis; 2010 Aug;48(8):505-11
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  • We report the expression pattern and construction of a transgenic zebrafish line for a transcription factor involved in otic vesicle formation and skeletogenesis.
  • Using bacterial artificial chromosome (BAC)-mediated transgenesis, we generated a zebrafish transgenic line for studying skeletal development, Tg(sp7:EGFP)b1212.
  • Using a zebrafish BAC, EGFP was introduced downstream of the regulatory regions of sp7 and injected into one cell-stage embryos.

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  • [Copyright] (c) 2010 Wiley-Liss, Inc.
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  • (PMID = 20506187.001).
  • [ISSN] 1526-968X
  • [Journal-full-title] Genesis (New York, N.Y. : 2000)
  • [ISO-abbreviation] Genesis
  • [Language] ENG
  • [Grant] United States / NIDCR NIH HHS / DE / DE013834-10; United States / NIDCD NIH HHS / DC / DC004186-13; United States / NICHD NIH HHS / HD / P01 HD022486-24; United States / NICHD NIH HHS / HD / P01 HD022486; United States / NIDCR NIH HHS / DE / DE019345-01; United States / NIDCR NIH HHS / DE / R01 DE013834; United States / NIDCD NIH HHS / DC / R01 DC004186-13; United States / NICHD NIH HHS / HD / HD022486-230002; United States / NIDCR NIH HHS / DE / R01 DE013834-10; United States / NIDCR NIH HHS / DE / F32 DE019345-01; United States / NIDCD NIH HHS / DC / R01 DC004186; United States / NICHD NIH HHS / HD / P01 HD022486-230002
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Osterix protein, zebrafish; 0 / RNA, Messenger; 0 / Transcription Factors; 0 / Zebrafish Proteins; 147336-22-9 / Green Fluorescent Proteins
  • [Other-IDs] NLM/ NIHMS206768; NLM/ PMC2926247
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98. Fontenot AP, Amicosante M: Metal-induced diffuse lung disease. Semin Respir Crit Care Med; 2008 Dec;29(6):662-9
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Metal-induced diffuse lung disease.
  • The number of metals that are associated with the development of diffuse parenchymal lung disease continues to expand.
  • In addition to lung fibrosis, inhalation of metal particulates can induce a wide range of lung pathology, including reactive airways disease and cancer.
  • More is known regarding the immunopathogenesis of beryllium-induced disease than is known for disease induced by any other metal.
  • Chronic beryllium disease (CBD) is a granulomatous lung disorder caused by beryllium exposure in the workplace and is characterized by the accumulation of beryllium-specific CD4 (+) T cells in the bronchoalveolar lavage.
  • Genetic susceptibility markers associated with increased risk have been identified for both CBD and hard metal lung disease.
  • The mechanism for the genetic susceptibility of CBD lies in the ability of certain human leukocyte antigen (HLA)-DP molecules to bind and present beryllium to pathogenic CD4 (+) T cells.
  • Whether the same is true for hard metal lung disease is unknown.
  • The study of metal-induced lung disease allows the investigation of the relationship between environmental exposure and genetic susceptibility.
  • These studies will enhance our understanding of the immunopathogenesis of metal-induced disease and how exposure to these metals results in irreversible lung fibrosis.
  • [MeSH-major] Beryllium / adverse effects. Cobalt / adverse effects. Lung Diseases, Interstitial / etiology
  • [MeSH-minor] Alloys / adverse effects. Berylliosis / etiology. Berylliosis / physiopathology. Chronic Disease. Genetic Predisposition to Disease. Humans. Inhalation Exposure / adverse effects. Occupational Diseases / etiology. Occupational Diseases / physiopathology. Occupational Exposure / adverse effects. Tungsten / adverse effects

  • MedlinePlus Health Information. consumer health - Interstitial Lung Diseases.
  • COS Scholar Universe. author profiles.
  • Hazardous Substances Data Bank. TUNGSTEN, ELEMENTAL .
  • Hazardous Substances Data Bank. BERYLLIUM, ELEMENTAL .
  • Hazardous Substances Data Bank. COBALT, ELEMENTAL .
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  • (PMID = 19221964.001).
  • [ISSN] 1098-9048
  • [Journal-full-title] Seminars in respiratory and critical care medicine
  • [ISO-abbreviation] Semin Respir Crit Care Med
  • [Language] eng
  • [Grant] United States / NIEHS NIH HHS / ES / ES011810; United States / NHLBI NIH HHS / HL / HL62410,; United States / NCRR NIH HHS / RR / M01 RR00051
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Review
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Alloys; 0 / hard metal; 3G0H8C9362 / Cobalt; OW5102UV6N / Beryllium; V9306CXO6G / Tungsten
  • [Number-of-references] 69
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99. Herrera MT, Torres M, Nevels D, Perez-Redondo CN, Ellner JJ, Sada E, Schwander SK: Compartmentalized bronchoalveolar IFN-gamma and IL-12 response in human pulmonary tuberculosis. Tuberculosis (Edinb); 2009 Jan;89(1):38-47
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Compartmentalized bronchoalveolar IFN-gamma and IL-12 response in human pulmonary tuberculosis.
  • Because concomitants of local Th1 immunity are still under-explored in humans, we characterized immune responses in bronchoalveolar cells (BACs) and systemically in peripheral blood mononuclear cells (PBMCs) in persons with active pulmonary TB and in healthy community controls.
  • PPD- and live M.tb-induced IFN-gamma-production were observed in CD4(+), CD8(+), gammadeltaTCR(+), and CD56(+) alveolar T cell subpopulations and NK cells (CD3(-)CD56(+)).
  • M.tb-induced IL-12p70, but interestingly also IL-4, was increased (p<0.05) in BACs from TB patients.
  • Constitutive expression of IL-12Rbeta1 and IL-12Rbeta2 mRNA in BACs and PBMCs and IFN-gammaR1 in BACs was similar in both study groups.
  • Data were normalized to account for differences in proportions of alveolar T cells and macrophages in the study groups.
  • IFN-gamma-production and its induction by IL-12R engagement occur virtually unimpaired in the bronchoalveolar spaces of patients with pulmonary TB.
  • The reasons for the apparent failure to control M. tuberculosis growth during active pulmonary TB disease is unknown but could be the expression of locally acting immunosuppressive mechanisms that subvert the antimycobacterial effects of IFN-gamma.