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1. Tachu B, Pillai S, Lucius R, Pogonka T: Essential role of chitinase in the development of the filarial nematode Acanthocheilonema viteae. Infect Immun; 2008 Jan;76(1):221-8
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Northern blot experiments and rapid amplification of cDNA ends-PCR with adult worms and larval stages showed that only one gene is expressed, with high variation in transcript levels, as determined by real-time PCR.
  • Chitinase transcript levels were lowest in the late male stage 4 larva (L4) and peaked in the stage 3 larva (L3), which was corroborated by Western blotting.
  • RNA interference (RNAi) experiments showed that treatment of L3 and adult female worms with double-stranded RNA of chitinase inhibited molting of L3 worms and hatching of microfilariae.

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  • (PMID = 17938220.001).
  • [ISSN] 1098-5522
  • [Journal-full-title] Infection and immunity
  • [ISO-abbreviation] Infect. Immun.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ EF052872/ EF052873/ U14638
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Helminth Proteins; EC 3.2.1.14 / Chitinase
  • [Other-IDs] NLM/ PMC2223640
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2. Giacomin PR, Wang H, Gordon DL, Botto M, Dent LA: Loss of complement activation and leukocyte adherence as Nippostrongylus brasiliensis develops within the murine host. Infect Immun; 2005 Nov;73(11):7442-9
NCI CPTAC Assay Portal. NCI CPTAC Assay Portal .

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Substantive deposition of C3 and adherence of eosinophil-rich leukocytes were seen with infective-stage (L3) but not with lung-stage (L4) larvae.
  • Adult intestinal worms had low to intermediate levels of both C3 and leukocyte binding.
  • For L3 and adult worms, complement deposition was principally dependent on the alternative pathway.

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  • (PMID = 16239545.001).
  • [ISSN] 0019-9567
  • [Journal-full-title] Infection and immunity
  • [ISO-abbreviation] Infect. Immun.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Complement C3; 0 / Complement C4; 80295-33-6 / Complement C1q; EC 3.4.21.47 / Complement Factor B
  • [Other-IDs] NLM/ PMC1273855
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3. Dixon H, Johnston CE, Else KJ: Antigen selection for future anti-Trichuris vaccines: a comparison of cytokine and antibody responses to larval and adult antigen in a primary infection. Parasite Immunol; 2008 Sep;30(9):454-61

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Antigen selection for future anti-Trichuris vaccines: a comparison of cytokine and antibody responses to larval and adult antigen in a primary infection.
  • Subcutaneous vaccination with adult excretory-secretory products (ES) protects susceptible mouse strains from T. muris.
  • Larval stages may contain novel and more relevant antigens which when incorporated in a vaccine induce worm expulsion earlier in infection than the adult worm products.
  • This study finds negligible difference in the cellular and humoral immune response to T. muris adult and third stage larva(e) (L3) ES during a primary T. muris infection, but identifies high molecular weight proteins in both adult and L3 ES as potential vaccine candidates.

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  • (PMID = 18565148.001).
  • [ISSN] 1365-3024
  • [Journal-full-title] Parasite immunology
  • [ISO-abbreviation] Parasite Immunol.
  • [Language] eng
  • [Grant] United Kingdom / Wellcome Trust / / 068028
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies, Helminth; 0 / Antigens, Helminth; 0 / Cytokines; 0 / Immunoglobulin G; 0 / Vaccines
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4. Murthy PK, Dixit S, Gaur RL, Kumar R, Sahoo MK, Shakya N, Joseph SK, Palne S, Gupta S: Influence of Brugia malayi life stages and BmAFII fraction on experimental Leishmania donovani infection in hamsters. Acta Trop; 2008 May;106(2):81-9
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The influence of live Brugia malayi parasites and a Sephadex G-200 fraction of the adult parasite extract (BmAFII) on the progression of Leishmania donovani infection was studied.
  • Inbred hamsters were first infected with B. malayi infective 3rd stage larvae (L3), adult worms or microfilariae (mf), and then with L. donovani amastigotes (Ld), or vice versa or received both the infections simultaneously; a group of animals were first immunized with BmAFII and then infected with Ld. L. donovani parasite burden was determined between 17 and 19 days post amastigote challenge (p.a.c.) and, in case of immunized animals, between 32 and 35 days p.a.c also.
  • Leishmanial parasite burden was significantly reduced in animals exposed to filarial L3 before amastigote inoculation and in animals given filarial adult worms after or together with amastigotes.
  • The findings show, for the first time, that B. malayi L3/adult worms or immunization with BmAFII inhibits progression of L. donovani infection in hamsters and this is associated with upregulation of NO and lymphocyte proliferative responses indicating that Th1 response might be responsible for this.

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  • (PMID = 18329620.001).
  • [ISSN] 0001-706X
  • [Journal-full-title] Acta tropica
  • [ISO-abbreviation] Acta Trop.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Antigens, Helminth; 31C4KY9ESH / Nitric Oxide
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5. Kiss JE, Gao X, Krepp JM, Hawdon JM: Interaction of hookworm 14-3-3 with the forkhead transcription factor DAF-16 requires intact Akt phosphorylation sites. Parasit Vectors; 2009 Apr 24;2(1):21
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  • BACKGROUND: Third-stage infective larvae (L3) of hookworms are in an obligatory state of developmental arrest that ends upon entering the definitive host, where they receive a signal that re-activates development.
  • Insulin-like signaling (ILS) mediates recovery from arrest in C. elegans and activation of hookworm dauer L3.
  • RESULTS: To determine if hookworm 14-3-3 proteins play a similar role in L3 activation, hookworm FTT-2 was identified and tested for its ability to interact with A. caninum DAF-16 in vitro.
  • Ac-FTT-2 was undetectable by Western blot in excretory/secretory products from serum-stimulated (activated) L3 or adult A. caninum.
  • CONCLUSION: The results indicate that Ac-FTT-2 interacts with DAF-16 in a phosphorylation-site dependent manner, and suggests that Ac-FTT-2 mediates activation of L3 by binding Ac-DAF-16 during hookworm infection.

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  • (PMID = 19393088.001).
  • [ISSN] 1756-3305
  • [Journal-full-title] Parasites & vectors
  • [ISO-abbreviation] Parasit Vectors
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / R01 AI069293; United States / NIAID NIH HHS / AI / R01 AI069293-03; United States / NIAID NIH HHS / AI / R01 AI069293-01A1; United States / NIAID NIH HHS / AI / AI069293-02; United States / NIAID NIH HHS / AI / R21 AI062857; United States / NIAID NIH HHS / AI / AI069293-01A1; United States / NIAID NIH HHS / AI / AI069293-03; United States / NIAID NIH HHS / AI / R01 AI069293-02
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Other-IDs] NLM/ PMC2683825
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6. Simcock DC, Brown S, Neale JD, Przemeck SM, Simpson HV: L3 and adult Ostertagia (Teladorsagia) circumcincta exhibit cyanide sensitive oxygen uptake. Exp Parasitol; 2006 Jan;112(1):1-7
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] L3 and adult Ostertagia (Teladorsagia) circumcincta exhibit cyanide sensitive oxygen uptake.
  • Oxygen consumption by L3 and adult Ostertagia (Teladorsagia) circumcincta was examined in vitro to determine whether oxygen can be utilised in metabolism.
  • Rates of consumption (in nmol O2/h/1000 worms) were 13+/-1 in sheathed L3, 34+/-6 in ex-sheathed L3, and 1944+/-495 in adult worms.
  • Consumption was inhibited 95% by cyanide in L3 and 74% in adults.

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  • (PMID = 16198342.001).
  • [ISSN] 0014-4894
  • [Journal-full-title] Experimental parasitology
  • [ISO-abbreviation] Exp. Parasitol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] IY9XDZ35W2 / Glucose; MQD255M2ZO / Potassium Cyanide; S88TT14065 / Oxygen
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7. Wang Z, Abubucker S, Martin J, Wilson RK, Hawdon J, Mitreva M: Characterizing Ancylostoma caninum transcriptome and exploring nematode parasitic adaptation. BMC Genomics; 2010;11:307
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • RESULTS: The generated transcripts from four developmental stages, infective L3, serum stimulated L3, adult male and adult female, covered 93% of the A. caninum transcriptome.
  • Examining the developmental expression profiles of A. caninum revealed major transitions in gene expression from larval stages to adult.
  • Adult males expressed the highest number of selectively expressed genes, but adult female expressed the highest number of selective parasitism-related genes.
  • Parasitism related genes were expressed more selectively in adult male and female worms.

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  • (PMID = 20470405.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Vaccines
  • [Other-IDs] NLM/ PMC2882930
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8. Geiger SM, Fujiwara RT, Santiago H, Corrêa-Oliveira R, Bethony JM: Early stage-specific immune responses in primary experimental human hookworm infection. Microbes Infect; 2008 Nov-Dec;10(14-15):1524-35

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • After an initial rise in proliferative responses against larval and adult worm antigens, the cellular reactivity decreased until the end of pre-patency, rose again during patency, and dropped after chemotherapy.
  • Before infection and during the course of infection, elevated concentrations of TNF-alpha were detected in supernatants of peripheral blood mononuclear cells (PBMC) stimulated in vitro with third-stage (L3) or adult worm excretory-secretory (ES) antigens, which dropped off after chemotherapy.
  • After stimulation with L3 antigen, elevated concentrations of CCL17 were detected in supernatants during pre-patency and patency.

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  • (PMID = 18848637.001).
  • [ISSN] 1286-4579
  • [Journal-full-title] Microbes and infection
  • [ISO-abbreviation] Microbes Infect.
  • [Language] ENG
  • [Grant] United States / FIC NIH HHS / TW / K01TW00009
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] France
  • [Chemical-registry-number] 0 / Antigens, Helminth; 0 / Chemokine CCL17; 0 / Tumor Necrosis Factor-alpha; 130068-27-8 / Interleukin-10
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9. Díaz-Camacho SP, Delgado-Vargas F, Willms K, de la Cruz-Otero Mdel C, Guadalupe Rendón-Maldonado J, Robert L, Antuna S, Nawa Y: Intrahepatic growth and maturation of Gnathostoma turgidum in the natural definitive opossum host, Didelphis virginiana. Parasitol Int; 2010 Sep;59(3):338-43

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The results show that tiny (<3mm in length) third stage larvae (L3) appeared in the liver of opossums around November and December.
  • Also in the liver, we found large L3 of up to about 10mm in length together with juveniles and mature adults from February to March.
  • In spite of their length, large L3 have 4 rows of hooklets, and their gonads remained undeveloped.
  • Morphological features of the small and large L3 of G. turgidum are described including scanning electron microscope images.
  • The seasonal switching of the several growth stages of G. turgidum from small L3 to adult worms in the liver and eventual migration to the stomach in opossums suggests the unique feature of G. turgidum utilizing the liver as the maturation site.

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  • (PMID = 20447468.001).
  • [ISSN] 1873-0329
  • [Journal-full-title] Parasitology international
  • [ISO-abbreviation] Parasitol. Int.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / DNA, Helminth; 0 / DNA, Ribosomal Spacer
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10. Jiang XJ, Wang JS, Fang Q: [Gene expression of breast cancer resistance protein in adult acute lymphocytic leukemia and its clinical significance]. Zhongguo Shi Yan Xue Ye Xue Za Zhi; 2008 Feb;16(1):31-4
Genetic Alliance. consumer health - Breast Cancer.

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] [Gene expression of breast cancer resistance protein in adult acute lymphocytic leukemia and its clinical significance].
  • The objective of this study was to investigate the relationship between the expressions of breast cancer resistance protein (BCRP) gene and drug resistance as well as prognosis in adult patients with acute lymphocytic leukemia (ALL).
  • Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of BCRP gene in 97 adult patients with acute lymphocytic leukemia (ALL) and 30 normal subjects.
  • In ALL subtypes BCRP gene expression of L3 type was distinctly higher than that of L(1), L(2) types.
  • It is concluded that the high expression of BCRP gene may induce clinical drug resistance, and may be an unfavorable factor for prognosis in adult patients with acute lymphocytic leukemia.

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  • (PMID = 18315895.001).
  • [ISSN] 1009-2137
  • [Journal-full-title] Zhongguo shi yan xue ye xue za zhi
  • [ISO-abbreviation] Zhongguo Shi Yan Xue Ye Xue Za Zhi
  • [Language] CHI
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / ABCG2 protein, human; 0 / Neoplasm Proteins
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11. Huber A, Prosl H, Joachim A, Simpson HV, Pedley KC: Effects of excretory/secretory products of Haemonchus contortus on cell vacuolation. Parasitol Res; 2005 Jul;96(5):290-5
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • We have used the latter approach to examine, at the cellular level, the effects of ES prepared from L3 and adult nematodes.
  • Both NR uptake and cellular vacuolation were increased following exposure to larval or adult ES products.
  • ES preparations from adult worms induced more extensive vacuolation then those from L3 worms and, as with VacA treatment, adherent cells remained viable despite high levels of vacuolation.

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  • (PMID = 15918071.001).
  • [ISSN] 0932-0113
  • [Journal-full-title] Parasitology research
  • [ISO-abbreviation] Parasitol. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Biological Factors; 0 / Helminth Proteins
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12. Kooyman FN, Ploeger HW, Höglund J, VAN Putten JP: Differential N-glycan- and protein-directed immune responses in Dictyocaulus viviparus-infected and vaccinated calves. Parasitology; 2007 Feb;134(Pt 2):269-79

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • In the search for protection-inducing antigens, we found that several D. viviparus third-stage larval (L3) and adult ES products carry N-glycans.
  • Challenge infection resulted in increased specific serum antibody levels against ES and L3 in the re-infected and challenge control groups.

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  • (PMID = 17032477.001).
  • [ISSN] 0031-1820
  • [Journal-full-title] Parasitology
  • [ISO-abbreviation] Parasitology
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antigens, Helminth; 0 / Immunodominant Epitopes; 0 / Polysaccharides
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13. Don TA, Oksov Y, Lustigman S, Loukas A: Saposin-like proteins from the intestine of the blood-feeding hookworm, Ancylostoma caninum. Parasitology; 2007 Mar;134(Pt 3):427-36

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Both Ac-slp-1 and slp-2 mRNAs were shown to be expressed in all life stages assessed, with slp-1 predominantly being expressed in third-stage larvae (L3) before and after activation with dog serum.
  • These antisera were used as probes in fluorescence microscopy to localize the anatomic expression sites of both proteins to small, punctate organelles or vesicles within the intestinal cells of adult worms; weak staining was detected on the microvillar brush border of the intestine.
  • Using transmission electron microscopy, both proteins were localized to similar vesicles in the intestinal cells of the L3.
  • Their expression in the gut of the L3 and adult stages suggests a role for these hookworm SAPLIPs in the lysis of host cells during tissue migration and/or feeding.

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  • (PMID = 17109779.001).
  • [ISSN] 0031-1820
  • [Journal-full-title] Parasitology
  • [ISO-abbreviation] Parasitology
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Helminth Proteins; 0 / RNA, Messenger; 0 / Recombinant Proteins; 0 / Saposins
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14. McVeigh P, Leech S, Marks NJ, Geary TG, Maule AG: Gene expression and pharmacology of nematode NLP-12 neuropeptides. Int J Parasitol; 2006 May 31;36(6):633-40
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Transcription was also identified in both L3 and adult stages of T. colubriformis, in which Tc-nlp-12 is expressed in a single tail neurone.
  • Conversely, As-nlp-12 is expressed in both head and tail tissue of adult female A. suum, suggesting species-specific differences in the transcription pattern of this gene.

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  • (PMID = 16600246.001).
  • [ISSN] 0020-7519
  • [Journal-full-title] International journal for parasitology
  • [ISO-abbreviation] Int. J. Parasitol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ AY347872/ DQ186899
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Caenorhabditis elegans Proteins; 0 / DNA, Complementary; 0 / DNA, Helminth; 0 / Helminth Proteins; 0 / Neuropeptides; 0 / aspartyl-tyrosyl-arginyl-prolyl-leucyl-glutaminyl-phenylalaninamide
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15. Dávila C, Malagón D, Valero A, Benítez R, Adroher FJ: Anisakis simplex: CO(2)-fixing enzymes and development throughout the in vitro cultivation from third larval stage to adult. Exp Parasitol; 2006 Sep;114(1):10-5
Hazardous Substances Data Bank. Carbon dioxide .

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Anisakis simplex: CO(2)-fixing enzymes and development throughout the in vitro cultivation from third larval stage to adult.
  • The results showed that a high pCO(2), at a suitable temperature, is vital for the optimum development of these nematodes, at least from the third larval stage (L3) to adult.
  • Throughout the development of the worms studied, PEPCK activity was much higher than that of PEPC (e.g., 305 vs. 6.8 nmol/min.mg protein, respectively, in L3 collected from the host fish).
  • The activity of these enzymes in the worms cultivated in air + 5% CO(2) was highest during M3, and was also generally higher than that of those cultivated in air only, especially during molting from L3 to L4 (e.g., in recently molted L4, PEPCK activity was 3.7 times greater than that of PEPC 2.9 times greater than when cultivated in air).

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  • (PMID = 16600219.001).
  • [ISSN] 0014-4894
  • [Journal-full-title] Experimental parasitology
  • [ISO-abbreviation] Exp. Parasitol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 142M471B3J / Carbon Dioxide; EC 4.1.1.31 / Phosphoenolpyruvate Carboxylase; EC 4.1.1.49 / Phosphoenolpyruvate Carboxykinase (ATP)
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16. García-Coiradas L, Angulo-Cubillán F, Méndez S, Larraga V, de la Fuente C, Cuquerella M, Alunda JM: Isolation and immunolocalization of a putative protective antigen (p26/23) from adult Haemonchus contortus. Parasitol Res; 2009 Jan;104(2):363-9

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • [Title] Isolation and immunolocalization of a putative protective antigen (p26/23) from adult Haemonchus contortus.
  • The putative protective antigen, p26/23, from adult Haemonchus contortus was isolated.
  • A soluble extract from adult helminths obtained from the abomasa of hyperinfected (12,000 infective larvae) female Manchego lambs and treated with a mixture of protease inhibitors was subjected to affinity chromatography (hexylgluthatione) to eliminate the enzyme gluthatione S-transferase.
  • This allowed the isolation of a single protein, which was expressed in both infective larvae (L3) and the adult stage of the parasite.

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  • (PMID = 18825413.001).
  • [ISSN] 0932-0113
  • [Journal-full-title] Parasitology research
  • [ISO-abbreviation] Parasitol. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antibodies, Helminth; 0 / Antigens, Helminth
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17. Bricarello PA, Zaros LG, Coutinho LL, Rocha RA, Silva MB, Kooyman FN, De Vries E, Yatsuda AP, Amarante AF: Immunological responses and cytokine gene expression analysis to Cooperia punctata infections in resistant and susceptible Nelore cattle. Vet Parasitol; 2008 Aug 1;155(1-2):95-103
NCI CPTC Antibody Characterization Program. NCI CPTC Antibody Characterization Program .

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Serum IgG1 mean levels against C. punctata antigens were higher in the resistant group, but significant differences between groups were only observed 14 days after the beginning of the experiment against infective larvae (L3) and 14 and 84 days against adult antigens.
  • The resistant group also presented higher IgA levels against C. punctata (L3 and adult) antigens with significant difference 14 days after the beginning of the trial (P<0.05).
  • In the small-intestine mucosa, levels of IgA anti-L3 and anti-adult C. punctata were higher in the resistant group, compared with the susceptible group (P<0.05).

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  • (PMID = 18513872.001).
  • [ISSN] 0304-4017
  • [Journal-full-title] Veterinary parasitology
  • [ISO-abbreviation] Vet. Parasitol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Cytokines; 0 / Immunoglobulin A
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18. Mary KA, Hoti SL, Paily KP: Monoclonal antibodies generated against excretory/secretory antigens of mammalian stage larvae of the lymphatic filarial parasite Wuchereria bancrofti. J Immunoassay Immunochem; 2007;28(4):343-57

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Five Mabs were obtained and, among them, Mab A7 showed high reactivity against e/s antigens of L4 and crude somatic antigens of microfilariae (mf) of W. bancrofti, and infective stage (L3) and adult stage larvae of Brugia malayi.

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  • (PMID = 17885888.001).
  • [ISSN] 1532-1819
  • [Journal-full-title] Journal of immunoassay & immunochemistry
  • [ISO-abbreviation] J Immunoassay Immunochem
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antibodies, Helminth; 0 / Antibodies, Monoclonal; 0 / Antigens, Helminth
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19. Iwata F, Shinjyo N, Amino H, Sakamoto K, Islam MK, Tsuji N, Kita K: Change of subunit composition of mitochondrial complex II (succinate-ubiquinone reductase/quinol-fumarate reductase) in Ascaris suum during the migration in the experimental host. Parasitol Int; 2008 Mar;57(1):54-61

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The flavoprotein (Fp) and small subunit of cytochrome b (CybS) in adult complex II differ from those of infective third stage larval (L3) complex II.
  • However, there is no difference in the iron-sulfur cluster (Ip) or the large subunit of cytochrome b (CybL) between adult and L3 isoforms of complex II.
  • In the present study, to clarify the changes that occur in the respiratory chain of A. suum larvae during their migration in the host, we examined enzymatic activity, quinone content and complex II subunit composition in mitochondria of lung stage L3 (LL3) A. suum larvae.
  • LL3 mitochondria showed higher QFR activity ( approximately 160 nmol/min/mg) than mitochondria of A. suum at other stages (L3: approximately 80 nmol/min/mg; adult: approximately 70 nmol/min/mg).
  • Interestingly, the results of two-dimensional bule-native/sodium dodecyl sulfate polyacrylamide gel electrophoresis analyses showed that LL3 mitochondria contained larval Fp (Fp(L)) and adult Fp (Fp(A)) at a ratio of 1:0.56, and that most LL3 CybS subunits were of the adult form (CybS(A)).

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  • (PMID = 17933581.001).
  • [ISSN] 1383-5769
  • [Journal-full-title] Parasitology international
  • [ISO-abbreviation] Parasitol. Int.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Antibodies, Helminth; 0 / Protein Subunits; 0 / Quinones; EC 1.- / Oxidoreductases; EC 1.3.5.1 / Electron Transport Complex II; EC 1.3.99.- / quinol fumarate reductase
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20. Brand AM, Varghese G, Majewski W, Hawdon JM: Identification of a DAF-7 ortholog from the hookworm Ancylostoma caninum. Int J Parasitol; 2005 Dec;35(14):1489-98
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Infective hookworm L3 encounter a host specific signal during invasion that re-activates suspended developmental pathways.
  • In the free-living nematode Caenorhabditis elegans, recovery from the developmentally arrested dauer stage in response to environmental cues is analogous to the resumption of development in invading hookworm L3.
  • An insulin-like signalling pathway mediates L3 activation in hookworms.
  • Sequence from a hookworm expressed sequence tag was used to design specific primers for PCR amplification of Ac-daf-7 from Ancylostoma caninum infective L3 cDNA.
  • Ac-daf-7 mRNA was strongly detected by reverse transcriptase PCR in L3 and serum stimulated L3 cDNA, and weakly in cDNA from L1 and adult life cycle stages.
  • Antiserum against Escherichia coli expressed recombinant Ac-DAF-7 detected the mature protein in L3 and adult soluble extracts, but not in excretory/secretory products from serum stimulated L3 or adults.
  • Increased expression in arrested L3 stages suggests that Ac-daf-7 is important for developmental arrest.

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  • (PMID = 16135366.001).
  • [ISSN] 0020-7519
  • [Journal-full-title] International journal for parasitology
  • [ISO-abbreviation] Int. J. Parasitol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ DQ058687/ DQ059758
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antigens, Helminth; 0 / Caenorhabditis elegans Proteins; 0 / DAF-7 protein, C elegans; 0 / Transforming Growth Factor beta
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21. Nisbet AJ, Bell NE, McNeilly TN, Knox DP, Maizels RM, Meikle LI, Wildblood LA, Matthews JB: A macrophage migration inhibitory factor-like tautomerase from Teladorsagia circumcincta (Nematoda: Strongylida). Parasite Immunol; 2010 Jul;32(7):503-11
The Lens. Cited by Patents in .

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • A cDNA representing Tci-mif-1 was isolated following its identification in third-stage larvae (L3)-enriched cDNA population.
  • Messenger RNA (mRNA) representing the Tci-MIF-1 transcript was detected in eggs, L3 and adult stages of T. circumcincta.
  • Using immunohistochemistry, the Tci-MIF-1 protein was shown to have a diffuse distribution in L3 tissue, and in L4 and adult stages, the protein was localized to the nematode gut.
  • A recombinant version of Tci-MIF-1 was produced, and enzymic assays indicated that this recombinant protein and a somatic extract of L3 possessed dopachrome tautomerase activity as has been observed previously in other MIF-like molecules.

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  • (PMID = 20591121.001).
  • [ISSN] 1365-3024
  • [Journal-full-title] Parasite immunology
  • [ISO-abbreviation] Parasite Immunol.
  • [Language] eng
  • [Grant] United Kingdom / Wellcome Trust / /
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA, Complementary; 0 / DNA, Helminth; 0 / Helminth Proteins; EC 5.3.- / Intramolecular Oxidoreductases; EC 5.3.3.12 / dopachrome isomerase
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22. Delaney A, Williamson A, Brand A, Ashcom J, Varghese G, Goud GN, Hawdon JM: Cloning and characterisation of an aspartyl protease inhibitor (API-1) from Ancylostoma hookworms. Int J Parasitol; 2005 Mar;35(3):303-13
NCI CPTC Antibody Characterization Program. NCI CPTC Antibody Characterization Program .

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Using sequence from the hookworm expressed sequence tag project, specific primers were designed and used to amplify Ac-api-1 from A. caninum infective L3 cDNA by PCR.
  • The A. ceylanicum api-1 cDNA of 878 bp was cloned from L3 cDNA using the A. caninum primers.
  • Ac-api-1 mRNA was detected by reverse transcriptase PCR in eggs, L1, L3 and adult life cycle stages.
  • A polyclonal antiserum against Escherichia coli expressed recombinant Ac-API-1 detected the protein in adult A. caninum excretory/secretory products, but not in those from activated infective larvae.
  • Immunolocalisation experiments using the antiserum indicated that Ac-API-1 is present primarily in the pseudocoelomic fluid in adult hookworms.
  • Soluble, yeast-expressed Ac-API-1 failed to inhibit pepsin or a hookworm gut aspartyl protease in vitro, but inhibited approximately 30% of the proteolytic activity of adult excretory/secretory products.
  • The pseudocoleomic location, presence in all life cycle stages, lack of inhibitory activity against pepsin, and inhibitory activity against excretory/secretory products suggest that Ac-API-1 inhibits an unidentified, putative aspartyl protease secreted by adult hookworms, and may be released as an enzyme-inhibitor complex.

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  • (PMID = 15722082.001).
  • [ISSN] 0020-7519
  • [Journal-full-title] International journal for parasitology
  • [ISO-abbreviation] Int. J. Parasitol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ AY705928/ AY705929
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antigens, Helminth; 0 / DNA, Complementary; 0 / DNA, Helminth; 0 / Protease Inhibitors; EC 3.4.23.- / Aspartic Acid Endopeptidases
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23. Wang WM, Guan YG, Liu F, Wang TH, Xu XY, Ke Q, Lu YC, Yuan Y: [The influence of partial dorsal root rhizotomy on IGF-I expression in spared root ganglion and spinal cord]. Sichuan Da Xue Xue Bao Yi Xue Ban; 2005 Jan;36(1):31-4

  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • OBJECTIVE: To explore the temporospatial changes of IGF-I expression in the spared dorsal root ganglia (DRG, L6) on the operated side and un-operated side, in the spinal lamina II (L3, L5, L6) and Clarke's nucleus (L3) of the adult cats that have undergone partial dorsal rhizotomy, and compare them against those of the normal adult cats so as to unveil the relation between IGF-I and the plasticity of spinal cord.
  • METHODS: Fifteen male adult cats were divided into three groups.
  • The bilateral L6 dorsal root ganglia and L3, L5, L6 spinal cord of all groups were made into frozen sections 20 microm thick.
  • The distribution and the number of IGF-I positive neurons in bilateral spared DRG (L6) on the operated/un-operated side, in spinal lamina I (L3, L5, L6) and in Clarke' nucleus (L3) of each animal were observed and counted.
  • On the 14th day, the IGF-I expression in neurons of L6 DRG on the operated side was significantly lower than that of normal group and that of L6 spared DRG on the unoperated side (P<0.01), but it was not significantly different from that of the 7th day group (P>0.05). (2) There was no difference in number of IGF-I positive neuron in L3, L5, L6 spinal lamina II between normal group, 7th day post-operation group and 14th day post-operation group (P>0.05).

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  • (PMID = 15702774.001).
  • [ISSN] 1672-173X
  • [Journal-full-title] Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition
  • [ISO-abbreviation] Sichuan Da Xue Xue Bao Yi Xue Ban
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 67763-96-6 / Insulin-Like Growth Factor I
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