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1. Berry SE, Sanders TA: Influence of triacylglycerol structure of stearic acid-rich fats on postprandial lipaemia. Proc Nutr Soc; 2005 May;64(2):205-12
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  • Previous research has focused on the quantity and composition of dietary fat, whereas the effect of triacylglycerol (TAG) structure on postprandial lipaemia and clotting factor VII activity has received little attention.
  • TAG with similar fatty acid composition may have different biochemical and physical properties that are dependent on their TAG structure, and these differences may affect lipid metabolism.
  • Recent findings suggest that differences in the physical properties of stearic acid-rich fats are associated with differences in postprandial lipaemia, and may play an important role in determining their rates of digestion and absorption.

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  • Hazardous Substances Data Bank. STEARIC ACID .
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  • (PMID = 15960865.001).
  • [ISSN] 0029-6651
  • [Journal-full-title] The Proceedings of the Nutrition Society
  • [ISO-abbreviation] Proc Nutr Soc
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Dietary Fats, Unsaturated; 0 / Lipids; 0 / Stearic Acids; 0 / Triglycerides; 4ELV7Z65AP / stearic acid; EC 3.4.21.21 / Factor VIIa
  • [Number-of-references] 63
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2. Numajiri K, Yamazaki T, Kimura M, Kuzuya A, Komiyama M: Discrete and active enzyme nanoarrays on DNA origami scaffolds purified by affinity tag separation. J Am Chem Soc; 2010 Jul 28;132(29):9937-9
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  • [Title] Discrete and active enzyme nanoarrays on DNA origami scaffolds purified by affinity tag separation.
  • Desired enzyme nanoarrays patterned on a DNA origami scaffold were selectively isolated by affinity tag purification from a pool of differently patterned nanoarrays, and their enzymatic activity was successfully confirmed.

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  • (PMID = 20590144.001).
  • [ISSN] 1520-5126
  • [Journal-full-title] Journal of the American Chemical Society
  • [ISO-abbreviation] J. Am. Chem. Soc.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Affinity Labels; 0 / Enzymes; 9007-49-2 / DNA
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3. Holen HL, Shadidi M, Narvhus K, Kjøsnes O, Tierens A, Aasheim HC: Signaling through ephrin-A ligand leads to activation of Src-family kinases, Akt phosphorylation, and inhibition of antigen receptor-induced apoptosis. J Leukoc Biol; 2008 Oct;84(4):1183-91
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  • Here, we show expression of ephrin-A ligands on CD4+ T cells cultured in medium with human serum and the T cell line Jurkat TAg and on cells isolated from patients with T cell lymphomas and T cell leukemias.
  • Functional role and identification of proteins involved in ephrin-A signaling were investigated here in the T cell line Jurkat TAg.
  • Interestingly, we show that signaling through ephrin-A in Jurkat TAg cells, initiated by interaction with the EphA2 receptor, leads to inhibition of activation-induced cell death.
  • To conclude, ephrin-A signaling in Jurkat TAg cells leads to induced phosphorylation of several proteins including Lck, Fyn, and Akt.


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4. Li YJ, Bi LJ, Zhang XE, Zhou YF, Zhang JB, Chen YY, Li W, Zhang ZP: Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip. Anal Bioanal Chem; 2006 Nov;386(5):1321-6
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  • [Title] Reversible immobilization of proteins with streptavidin affinity tags on a surface plasmon resonance biosensor chip.
  • This paper describes a new protocol for reversible and site-directed immobilization of proteins with streptavidin affinity tags on the streptavidin-coated SPR biosensor chip (SA chip).
  • Two streptavidin affinity tags, nano-tag and streptavidin-binding peptide (SBP tag), were applied.
  • The fusion construct of a SBP tag and a single-chain antibody to mature bovine prion protein (scFv-Z186-SBP) interacts with the SA chip, resulting in a single-chain-antibody-modified surface.

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  • (PMID = 17006676.001).
  • [ISSN] 1618-2642
  • [Journal-full-title] Analytical and bioanalytical chemistry
  • [ISO-abbreviation] Anal Bioanal Chem
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Immunoglobulin Variable Region; 0 / Peptides; 0 / Prions; 9013-20-1 / Streptavidin
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5. Coort SL, Coumans WA, Bonen A, van der Vusse GJ, Glatz JF, Luiken JJ: Divergent effects of rosiglitazone on protein-mediated fatty acid uptake in adipose and in muscle tissues of Zucker rats. J Lipid Res; 2005 Jun;46(6):1295-302
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  • The increase in FA uptake capacity of adipose tissue was associated with a decline in plasma FA and triacylglycerols (TAGs), suggesting that Rgz treatment enhanced plasma FA extraction by adipocytes.
  • In obese hearts, Rgz treatment had no effect on the FA transport system, yet the total TAG content decreased, suggesting enhanced insulin sensitivity.
  • However, the TAG content remained unaltered in skeletal muscle, which coincided with increased cytoplasmic adipose-type FABP content, suggesting that increased extramyocellular TAGs mask the decline of intracellular TAG in muscle.

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  • (PMID = 15772429.001).
  • [ISSN] 0022-2275
  • [Journal-full-title] Journal of lipid research
  • [ISO-abbreviation] J. Lipid Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, CD36; 0 / Fatty Acid Transport Proteins; 0 / Fatty Acids; 0 / Hypoglycemic Agents; 0 / Membrane Transport Proteins; 0 / Thiazolidinediones; 0 / Triglycerides; 05V02F2KDG / rosiglitazone; 2V16EO95H1 / Palmitic Acid
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6. Chapman J, Clayton D: One degree of freedom for dominance in indirect association studies. Genet Epidemiol; 2007 Apr;31(3):261-71
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  • Usual tests of association using tag single nucleotide polymorphisms (SNPs) assume that the alleles of the causal locus act additively and that these alleles are then predicted indirectly via a set of tag SNPs.
  • In the presence of strong dominance effects this model is not correct and an extra term needs to be included, which uses the tag SNPs to predict the heterozygosity of the causal locus.
  • Assuming this scenario of a strong dominance effect, we present an appropriate test statistic and investigate how much power, if any, we gain by adding this single degree of freedom for dominance.

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  • (PMID = 17266117.001).
  • [ISSN] 0741-0395
  • [Journal-full-title] Genetic epidemiology
  • [ISO-abbreviation] Genet. Epidemiol.
  • [Language] eng
  • [Grant] United Kingdom / Medical Research Council / / G0601942
  • [Publication-type] Journal Article
  • [Publication-country] United States
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7. Hsiao YY, Lin CH, Liu JK, Wong TY, Kuo J: Analysis of Codon Usage Patterns in Toxic Dinoflagellate Alexandrium tamarense through Expressed Sequence Tag Data. Comp Funct Genomics; 2010;2010:138538
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  • [Title] Analysis of Codon Usage Patterns in Toxic Dinoflagellate Alexandrium tamarense through Expressed Sequence Tag Data.
  • We have analyzed synonymous codon usage in the genome of A. tamarense CCMP 1598 for protein-coding sequences from 10865 expressed sequence tags (ESTs).
  • Correspondence analysis of A. tamarense genes based on codon usage showed that the GC content at the third base of synonymous codons (GC3s) was strongly correlated with the first axis (r = 0.93 with P < .001).

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  • (PMID = 21052492.001).
  • [ISSN] 1532-6268
  • [Journal-full-title] Comparative and functional genomics
  • [ISO-abbreviation] Comp. Funct. Genomics
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Egypt
  • [Other-IDs] NLM/ PMC2967832
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8. Hjortø GM, Hansen M, Larsen NB, Kledal TN: Generating substrate bound functional chemokine gradients in vitro. Biomaterials; 2009 Oct;30(29):5305-11
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  • Here, indirect mCP of a capture antibody recognizing a molecular tag on the target protein is successfully used to pattern tagged fractalkine in microscale gradient patterns.
  • The use of a capture antibody facilitates control of the orientation of tagged molecules, thereby ensuring a high degree of bio-functionality through correct presentation and reduced protein denaturation.

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  • (PMID = 19577290.001).
  • [ISSN] 1878-5905
  • [Journal-full-title] Biomaterials
  • [ISO-abbreviation] Biomaterials
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Chemokine CX3CL1; 0 / Coated Materials, Biocompatible
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9. Schubert S, Schnabel KP, Winkelmann A: Assessment of spatial anatomical knowledge with a 'three-dimensional multiple choice test' (3D-MC). Med Teach; 2009 Jan;31(1):e13-7
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  • An alternative are the "spotters" or "tag tests" well-known in North American and British anatomy.
  • AIMS: To describe the implementation and feasibility of a multiple choice "tag test" (3D-MC) using prosected specimens, histological slides, models and radiographs; to report the psychometric properties and students' acceptance of the 3D-MC; to compare it with a traditional multiple choice format.

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  • (PMID = 19253152.001).
  • [ISSN] 1466-187X
  • [Journal-full-title] Medical teacher
  • [ISO-abbreviation] Med Teach
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] England
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10. Ferreiro-Neira I, Calaza M, Alonso-Perez E, Marchini M, Scorza R, Sebastiani GD, Blanco FJ, Rego I, Pullmann R Jr, Pullmann R, Kallenberg CG, Bijl M, Skopouli FN, Mavromati M, Migliaresi S, Barizzone N, Ruzickova S, Dostal C, Schmidt RE, Witte T, Papasteriades C, Kappou-Rigatou I, Endreffy E, Kovacs A, Ordi-Ros J, Balada E, Carreira P, Gomez-Reino JJ, Gonzalez A: Opposed independent effects and epistasis in the complex association of IRF5 to SLE. Genes Immun; 2007 Jul;8(5):429-38
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  • Eleven polymorphisms were studied, including nine tag single nucleotide polymorphisms (SNPs) and two extra functional polymorphisms.
  • Two tag SNPs showed independent and opposed associations: susceptibility (rs10488631, P<10(-17)) and protection (rs729302, P<10(-6)).

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  • (PMID = 17568788.001).
  • [ISSN] 1466-4879
  • [Journal-full-title] Genes and immunity
  • [ISO-abbreviation] Genes Immun.
  • [Language] eng
  • [Databank-accession-numbers] OMIM/ 152700/ 607218
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / IRF5 protein, human; 0 / Interferon Regulatory Factors
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11. Haroun M, Ravelet C, Ravel A, Grosset C, Villet A, Peyrin E: Thermodynamic origin of the chiral recognition of tryptophan on teicoplanin and teicoplanin aglycone stationary phases. J Sep Sci; 2005 Mar;28(5):409-20
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  • The D-, L-tryptophan binding and the chiral recognition properties of the teicoplanin and teicoplanin aglycone (TAG) chiral stationary phase (CSPs) were compared at various column temperatures.
  • The change in the enantioselective process observed between the TAG and teicoplanin CSP was characterized by a difference of ca.

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  • (PMID = 15835728.001).
  • [ISSN] 1615-9306
  • [Journal-full-title] Journal of separation science
  • [ISO-abbreviation] J Sep Sci
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Germany
  • [Chemical-registry-number] 61036-62-2 / Teicoplanin; 8DUH1N11BX / Tryptophan
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12. Kim JH, Chang TM, Graham AN, Choo KH, Kalitsis P, Hudson DF: Streptavidin-Binding Peptide (SBP)-tagged SMC2 allows single-step affinity fluorescence, blotting or purification of the condensin complex. BMC Biochem; 2010;11:50
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  • RESULTS: We have tagged the SMC2 subunit of the condensin complex with the Streptavidin-Binding Peptide (SBP), optimising and demonstrating the efficacious use of this tag for performing these protein analytical steps.
  • Furthermore we have shown that the SBP tag can be used for isolating tagged proteins from chemically cross-linked cell populations for capturing DNA-protein interactions.

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  • (PMID = 21194474.001).
  • [ISSN] 1471-2091
  • [Journal-full-title] BMC biochemistry
  • [ISO-abbreviation] BMC Biochem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Carrier Proteins; 0 / DNA-Binding Proteins; 0 / Multiprotein Complexes; 0 / Nuclear Proteins; 0 / Recombinant Fusion Proteins; 0 / SMC2 protein, human; 0 / condensin complexes; 0 / streptavidin-binding peptide; 9013-20-1 / Streptavidin; EC 3.6.1.- / Adenosine Triphosphatases
  • [Other-IDs] NLM/ PMC3022668
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13. Zivković M, Stanković A, Dincić E, Popović M, Popović S, Raicević R, Alavantić D: The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: cost-effective high-throughput detection by real-time PCR. Clin Chim Acta; 2009 Aug;406(1-2):27-30
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  • [Title] The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: cost-effective high-throughput detection by real-time PCR.
  • BACKGROUND: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region.

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  • (PMID = 19433080.001).
  • [ISSN] 1873-3492
  • [Journal-full-title] Clinica chimica acta; international journal of clinical chemistry
  • [ISO-abbreviation] Clin. Chim. Acta
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / HLA-DR Antigens; 0 / HLA-DRB1 Chains; 0 / HLA-DRB1*15:01 antigen; EC 2.7.7.- / Taq Polymerase
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14. Huntley D, Baldo A, Johri S, Sergot M: SEAN: SNP prediction and display program utilizing EST sequence clusters. Bioinformatics; 2006 Feb 15;22(4):495-6
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  • SEAN is an application that predicts single nucleotide polymorphisms (SNPs) using multiple sequence alignments produced from expressed sequence tag (EST) clusters.
  • [MeSH-major] DNA Mutational Analysis / methods. Expressed Sequence Tags. Polymorphism, Single Nucleotide / genetics. Sequence Alignment / methods. Sequence Analysis, DNA / methods. Software. User-Computer Interface

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  • (PMID = 16357032.001).
  • [ISSN] 1367-4803
  • [Journal-full-title] Bioinformatics (Oxford, England)
  • [ISO-abbreviation] Bioinformatics
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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15. Wu WY, Mee C, Califano F, Banki R, Wood DW: Recombinant protein purification by self-cleaving aggregation tag. Nat Protoc; 2006;1(5):2257-62
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  • [Title] Recombinant protein purification by self-cleaving aggregation tag.
  • This method is based on a reversibly precipitating, self-cleaving purification tag.
  • The tag is made up of two components: an elastin-like polypeptide (ELP), which reversibly self-associates in high-salt buffers at temperatures above 30 degrees C; and an intein, which causes the ELP tag to self-cleave in response to a mild pH shift.
  • Once purified, intein-mediated self-cleavage, followed by precipitation of the cleaved ELP tag, allows easy and effective isolation of the pure, native target protein without the need for chromatographic separations.

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  • (PMID = 17406465.001).
  • [ISSN] 1750-2799
  • [Journal-full-title] Nature protocols
  • [ISO-abbreviation] Nat Protoc
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Peptides; 0 / Recombinant Proteins; 147336-22-9 / Green Fluorescent Proteins
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16. Wakuta S, Hamada S, Ito H, Matsuura H, Nabeta K, Matsui H: Identification of a beta-glucosidase hydrolyzing tuberonic acid glucoside in rice (Oryza sativa L.). Phytochemistry; 2010 Aug;71(11-12):1280-8
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  • [Title] Identification of a beta-glucosidase hydrolyzing tuberonic acid glucoside in rice (Oryza sativa L.).
  • Tuberonic acid (TA) and its glucoside (TAG) have been isolated from potato (Solanum tuberosum L.) leaflets and shown to exhibit tuber-inducing properties.
  • Here we describe the in vivo hydrolytic activity of TAG in rice.
  • In this study, the TA resulting from TAG was not converted into JA.
  • Tuberonic acid glucoside (TAG)-hydrolyzing beta-glucosidase, designated OsTAGG1, was purified from rice by six purification steps with an approximately 4300-fold purification.
  • In the native enzyme, the K(m) and V(max) values of TAG were 31.7 microM and 0.25 microkatal/mg protein, OsTAGG1 preferentially hydrolyzed TAG and methyl TAG.
  • Here we report that OsTAGG1 is a specific beta-glucosidase hydrolyzing TAG, which releases the physiologically active TA.

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  • [Copyright] Copyright 2010 Elsevier Ltd. All rights reserved.
  • (PMID = 20570296.001).
  • [ISSN] 1873-3700
  • [Journal-full-title] Phytochemistry
  • [ISO-abbreviation] Phytochemistry
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Acetates; 0 / Cyclopentanes; 0 / Glucosides; 0 / Oxylipins; 0 / tuberonic acid glucoside; 124649-26-9 / tuberonic acid; 6RI5N05OWW / jasmonic acid; EC 3.2.1.21 / beta-Glucosidase
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17. Cao J, Arulanandam R, Vultur A, Anagnostopoulou A, Raptis L: Differential effects of c-Ras upon transformation, adipocytic differentiation, and apoptosis mediated by the simian virus 40 large tumor antigen. Biochem Cell Biol; 2007 Feb;85(1):32-48
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  • To investigate the functional relationship between the ability of the simian virus 40 large tumor antigen (TAg) to transform and its ability to block adipocytic differentiation and induce apoptosis, we expressed TAg in C3H10T1/2 (10T1/2)-derived preadipocytes.
  • The results demonstrated that differentiation could be suppressed at lower TAg levels than at the levels required for full neoplastic conversion.
  • Progressively higher TAg levels were accompanied by apoptosis induction in this system.
  • To further examine the role of the cellular Ras protooncogene product (Ras) in TAg function, TAg was expressed in 10T1/2-derived preadipocytes rendered deficient in Ras activity by transfection with inducible or constitutive antisense ras gene constructs.
  • The results indicated that Ras is required for TAg-mediated transformation and for suppression of adipocytic differentiation, while TAg-mediated apoptosis following serum starvation was independent from Ras action.
  • Unexpectedly, our results further demonstrated a dramatic reduction in the levels of the TAg protein itself as differentiation progressed in Ras-knockdown cells, with a concomitant reduction in TAg's ability to induce apoptosis as a result.
  • These findings suggest that Ras, although cytoplasmic, is an integral component of the pathway whereby TAg, an oncoprotein believed to have primarily nuclear targets, suppresses differentiation or induces neoplastic conversion of murine preadipocytes.

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  • [ErratumIn] Biochem Cell Biol. 2008 Apr;86(2):214-5
  • [ErratumIn] Biochem Cell Biol. 2007 Jun;85(3):395. Anagnostopoulu, Aikaterini [corrected to Anagnostopoulou, Aikaterini]
  • (PMID = 17464343.001).
  • [ISSN] 0829-8211
  • [Journal-full-title] Biochemistry and cell biology = Biochimie et biologie cellulaire
  • [ISO-abbreviation] Biochem. Cell Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Canada
  • [Chemical-registry-number] 0 / Antigens, Viral, Tumor; EC 3.6.5.2 / Proto-Oncogene Proteins p21(ras)
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18. Dimaras H, Gallie BL: The p75 NTR neurotrophin receptor is a tumor suppressor in human and murine retinoblastoma development. Int J Cancer; 2008 May 01;122(9):2023-9
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  • The transition from the benign retinal tumor retinoma to its malignant counterpart retinoblastoma is accompanied by the loss of expression of the p75(NTR) neurotrophin receptor.
  • This change in expression is mimicked in the TAg-RB murine model of retinoblastoma, where early tumors retain expression of p75(NTR) and advanced tumors lack it.
  • We sought to determine the functional effect on tumor development of absence of p75(NTR) from the onset of TAg-RB tumor initiation.
  • TAg-RB mice were crossed with either p75(NTR) exon 3 (E3KO) or exon 4 knockout (E4KO) mice to produce TAg-RB offspring that lacked one or both normal p75(NTR) alleles.
  • TAg-RB/E3KO (TAg-RB(E3KO)) and heterozygous mice showed no significant difference in tumor area compared to the TAg-RB control mice at any time point studied.
  • However, TAg-RB/E4KO (TAg-RB(E4KO)) and heterozygous mice displayed a significantly larger tumor area than the TAg-RB control mice.
  • Our results confirm that p75(NTR) suppresses progression of both human and TAg-RB murine retinoblastoma, and holds promise as a target for future therapy of the disease.

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  • [Copyright] (c) 2008 Wiley-Liss, Inc.
  • (PMID = 18196575.001).
  • [ISSN] 1097-0215
  • [Journal-full-title] International journal of cancer
  • [ISO-abbreviation] Int. J. Cancer
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / R01 CA118830
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Receptor, Nerve Growth Factor; 0 / Tumor Suppressor Proteins; EC 3.4.22.- / Caspase 3
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19. Obermayer B, Frey E: Error thresholds for self- and cross-specific enzymatic replication. J Theor Biol; 2010 Dec 21;267(4):653-62
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  • The information content of a non-enzymatic self-replicator is limited by Eigen's error threshold.
  • Because specific recognition can enforce these propensities, we thoroughly analyze an idealized quasispecies model for enzymatic replication, with replication rates that are either a decreasing (self-specific) or increasing (cross-specific) function of the Hamming distance between the recognition or "tag" sequences of enzyme and substrate.
  • Extrapolating from experimental data, we obtain rough quantitative estimates for the maximal length of the recognition or tag sequence that can be used to reliably discriminate appropriate and infeasible enzymes and substrates, respectively.

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  • [Copyright] Copyright © 2010 Elsevier Ltd. All rights reserved.
  • (PMID = 20863836.001).
  • [ISSN] 1095-8541
  • [Journal-full-title] Journal of theoretical biology
  • [ISO-abbreviation] J. Theor. Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Enzymes
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20. Teng LF, Lin F, Zheng MY, Zheng CH, Wu F, Zeng AP, Huang EP, Mo YH, Zheng MQ, Li XY, Hou JY: [Expression of recombinant VP2 gene in insect sf9 cells and screening of clinical specimens]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi; 2009 Dec;23(6):427-9
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  • The fusion protein with HA tag was applied to confirm the specificity of expressed protein.
  • Through HA tag the VP2 protein was specific, and then the assay using SDS-PAGE with Western blot could detect and screen the antibody in serum from children with lower respiratory tract infections rapidly and accurately.
  • [MeSH-major] Bocavirus / genetics. Capsid Proteins / genetics. Gene Expression. Parvoviridae Infections / diagnosis

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  • (PMID = 20718346.001).
  • [ISSN] 1003-9279
  • [Journal-full-title] Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology
  • [ISO-abbreviation] Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Antibodies, Viral; 0 / Capsid Proteins; 0 / Recombinant Proteins
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21. Morrow JM, Chang BS: The p1D4-hrGFP II expression vector: a tool for expressing and purifying visual pigments and other G protein-coupled receptors. Plasmid; 2010 Nov;64(3):162-9
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  • This expression vector is based on pIRES-hrGFP II (Stratagene), with the addition of a C-terminal 1D4 epitope tag for immunoblotting and immunoaffinity purification.

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  • [Copyright] Copyright © 2010 Elsevier Inc. All rights reserved.
  • (PMID = 20627111.001).
  • [ISSN] 1095-9890
  • [Journal-full-title] Plasmid
  • [ISO-abbreviation] Plasmid
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Cone Opsins; 0 / Receptors, G-Protein-Coupled; 0 / Zebrafish Proteins; 63231-63-0 / RNA; 9009-81-8 / Rhodopsin
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22. Yang B, Kallio H: Analysis of triacylglycerols of seeds and berries of sea buckthorn (Hippophaë rhamnoides) of different origins by mass spectrometry and tandem mass spectrometry. Lipids; 2006 Apr;41(4):381-92
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  • TAG of seeds, berries, and fruit pulp/peel of different subspecies of sea buckthorn (Hippophaë rhamnoides) were analyzed by MS and tandem mass spectrometry (MS/MS).
  • The seeds contained mainly TAG with acyl carbon number (ACN) of 52 with 2-6 double bonds (DB) (20-30%), and TAG of ACN 54 with 3-9 DB (70-80%).
  • In the pulp/peel fraction, the major TAG were species with ACN:DB of 48:1 to 48:3 (19-49%), 50:1 to 50:4 (31-41%), and 52:1 to 52:6 (9-19%).
  • Subspecies (ssp.) sinensis differed from ssp. mongolica and rhamnoides by having a higher proportion of TAG of ACN 52 (27% vs. 21% and 22%, P< 0.05) and a lower proportion of ACN 54 (71% vs. 79% and 78%, P < 0.01) in seed TAG.
  • Seed TAG of ssp. mongolica contained a higher proportion of more unsaturated species compared with those of the two other subspecies.
  • Berry TAG of ssp. mongolica had the highest proportion of molecular species of ACN 48 due to the higher proportion of palmitic and palmitoleic acids and the lower seed content of the berries.
  • The order of preference of unsaturated FA for the sn-2 position depended at least partially on the FA combination of TAG.
  • Seed TAG of ssp. mongolica contained a higher proportion of alpha-linolenic acid in the sn-2 position than those of ssp. sinensis.
  • In berry TAG, ssp. mongolica had the highest proportions of palmitoleic and linoleic acids in the sn-2 position, and the lowest proportion of oleic/cis-vaccenic acid in the sn-2 position, among the three subspecies.

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  • [Cites] Lipids. 1993 Mar;28(3):217-22 [8464352.001]
  • [Cites] J Agric Food Chem. 2001 Apr;49(4):1939-47 [11308350.001]
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  • (PMID = 16808152.001).
  • [ISSN] 0024-4201
  • [Journal-full-title] Lipids
  • [ISO-abbreviation] Lipids
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Fatty Acids; 0 / Triglycerides
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23. Wikström A, Deinum J: Probing the interaction of coagulation factors with phospholipid vesicle surfaces by surface plasma resonance. Anal Biochem; 2007 Mar 1;362(1):98-107
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  • The diameter in solution was 145 nm, but the more DNA tags/vesicle the more compressed the immobilized vesicles became; with 30 DNA tags the calculated thickness was 88 nm and with 1 DNA tag it was 138 nm.
  • However, compression of the vesicles had no effect on the K(D).
  • In contrast, the rate constants were dependent on the number of DNA tags; thus deformation of the vesicles was observed.
  • The k(a) and k(d) for FXa were similar for vesicles attached with 30 DNA tags or fused on the L1 chip but higher with fewer tags and approximately 10 times higher if attached with 1 tag.

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  • (PMID = 17239338.001).
  • [ISSN] 0003-2697
  • [Journal-full-title] Analytical biochemistry
  • [ISO-abbreviation] Anal. Biochem.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Blood Coagulation Factors; 0 / Phospholipids; 9007-49-2 / DNA
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24. Amor-Mahjoub M, Suppini JP, Gomez-Vrielyunck N, Ladjimi M: The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs. J Chromatogr B Analyt Technol Biomed Life Sci; 2006 Dec 5;844(2):328-34
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  • [Title] The effect of the hexahistidine-tag in the oligomerization of HSC70 constructs.
  • The hexahistidine is a fusion tag used for the isolation of proteins via an immobilized metal-ion affinity chromatography (IMAC).
  • In the present study, we have purified and analyzed two constructs of the heat shock protein HSC70 in the presence or the absence of the His-tag (C30WT-His(+)/C30WT and C30DeltaL-His(+)/C30DeltaL).
  • Results from SEC analysis indicated that the His-tag promotes the dimerization of C30DeltaL-His(+) but has no effect on the elution profile of C30WT-His(+), compared to their respective untagged forms C30DeltaL and C30WT.
  • These observations were also confirmed by AU analysis which indicates that C30DeltaL is stabilized in the dimeric form in the presence of the His-tag.
  • These results emphasize the need to remove the His-tag before structural characterization of some recombinant proteins.

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  • (PMID = 16904956.001).
  • [ISSN] 1570-0232
  • [Journal-full-title] Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • [ISO-abbreviation] J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / HSP70 Heat-Shock Proteins; 0 / His-His-His-His-His-His; 0 / Mutant Proteins; 0 / Oligopeptides; 0 / Recombinant Proteins; 4QD397987E / Histidine
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25. Thevissen PW, Poelman G, De Cooman M, Puers R, Willems G: Implantation of an RFID-tag into human molars to reduce hard forensic identification labor. Part 2: physical properties. Forensic Sci Int; 2006 May 15;159 Suppl 1:S40-6
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  • [Title] Implantation of an RFID-tag into human molars to reduce hard forensic identification labor. Part 2: physical properties.
  • Modifying and implanting existing RFID-tags into extracted human molars was described previously [P.
  • Willems, Implantation of RFID-tag into human molars to reduce hard forensic identification labor. Part 1.
  • Maximal vertical occlusal load during which the implanted RFID-tags remain active was evaluated in vitro.
  • The temperature dependency of the implanted RFID-tags was studied on the extracted teeth to find out the maximal obtainable temperature before failure and a test with embedded thermistors to verify temperature distributions.
  • The maximal working temperature of the implanted RFID-tags was revealed and gave indications for the set-up of measuring intra oral and intra tooth temperature during the cremation of a human body.
  • The results of this investigation showed the need of putting an extra insulating layer around the modified tags before implantation.
  • The different in vitro tests indicated that the implanted RFID-tags can support certain oral and forensic circumstances.

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  • (PMID = 16554132.001).
  • [ISSN] 0379-0738
  • [Journal-full-title] Forensic science international
  • [ISO-abbreviation] Forensic Sci. Int.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Ireland
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26. Vallelian F, Schaer CA, Kaempfer T, Gehrig P, Duerst E, Schoedon G, Schaer DJ: Glucocorticoid treatment skews human monocyte differentiation into a hemoglobin-clearance phenotype with enhanced heme-iron recycling and antioxidant capacity. Blood; 2010 Dec 9;116(24):5347-56
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  • Using global proteome and transcriptome analysis with mass-spectrometry (isobaric tag for relative and absolute quantitation and liquid chromatography-mass spectrometry) and gene-array experiments, we found that glucocorticoid treatment in vitro and in patients on glucocorticoid-pulse therapy polarized monocytes into a M2/alternatively activated phenotype with high Hb-scavenger receptor (CD163) expression and enhanced Hb-clearance and detoxification capability.
  • Monocytes concurrently exposed to the interactive activity of glucocorticoids and extracellular Hb were characterized by high expression of a group of antioxidant enzymes known to be regulated by the conserved oxidative response transcription factor nuclear factor E2-related factor.

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  • (PMID = 20739658.001).
  • [ISSN] 1528-0020
  • [Journal-full-title] Blood
  • [ISO-abbreviation] Blood
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, CD; 0 / Antigens, Differentiation, Myelomonocytic; 0 / Antioxidants; 0 / CD163 antigen; 0 / Glucocorticoids; 0 / Hemoglobins; 0 / Receptors, Cell Surface; 42VZT0U6YR / Heme; E1UOL152H7 / Iron
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27. Shedden K, Rosania GR: Chemical address tags of fluorescent bioimaging probes. Cytometry A; 2010 May;77(5):429-38
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  • [Title] Chemical address tags of fluorescent bioimaging probes.
  • Chemical address tags can be defined as specific structural features shared by a set of bioimaging probes having a predictable influence on cell-associated visual signals obtained from these probes.
  • Here, using a large image dataset acquired with a high content screening instrument, machine vision and cheminformatics analysis have been applied to reveal chemical address tags.
  • In this manner, variations in the chemical features of the molecules were associated with the resulting staining patterns, facilitating quantitative, objective analysis of chemical address tags.
  • Inspection of the clustered structures suggests intramolecular charge migration or partial charge distribution as potential mechanistic determinants of chemical address tag behavior.

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  • (PMID = 20104576.001).
  • [ISSN] 1552-4930
  • [Journal-full-title] Cytometry. Part A : the journal of the International Society for Analytical Cytology
  • [ISO-abbreviation] Cytometry A
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / GM078200-05; United States / NIGMS NIH HHS / GM / R01 GM078200-05; United States / NIGMS NIH HHS / GM / GM078200-04; United States / NIGMS NIH HHS / GM / R01 GM078200-04; United States / NIGMS NIH HHS / GM / R01 GM078200; United States / NIGMS NIH HHS / GM / R01 GM078200-03; United States / NIGMS NIH HHS / GM / GM078200-03
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Aldehydes; 0 / Fluorescent Dyes; 0 / Pyridinium Compounds; 0 / Quinolinium Compounds
  • [Other-IDs] NLM/ NIHMS203298; NLM/ PMC2907078
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28. Deng H, Jiang Q, Liang S, Yan F, Hou S, Qian Z, Li J, Wen Y, Yang J, Wei Y: Prokaryotic expression, purification and characterization of a novel pro-apoptosis protein hPNAS-4. Biotechnol Appl Biochem; 2010 Feb;55(2):63-72
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  • [Title] Prokaryotic expression, purification and characterization of a novel pro-apoptosis protein hPNAS-4.
  • In our present studies, the hPNAS-4 gene was first cloned into the pGEX-6p-1 vector with a GST (glutathione transferase) tag to express in soluble form in Escherichia coli induced with 0.5 mM IPTG (isopropyl beta-D-thiogalactoside) at 25 degrees C, and the recombinant hPNAS-4 was purified to near homogeneity with 96% purity by affinity chromatography and anion-exchange chromatography.

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  • (PMID = 19912110.001).
  • [ISSN] 1470-8744
  • [Journal-full-title] Biotechnology and applied biochemistry
  • [ISO-abbreviation] Biotechnol. Appl. Biochem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies; 0 / Apoptosis Regulatory Proteins; 0 / PNAS4 protein, human; 0 / Recombinant Fusion Proteins
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29. Cao S, Liu Z, Guo A, Li Y, Zhang C, Gaobing W, Chunfang F, Tan Y, Chen H: Efficient production and characterization of Bacillus anthracis lethal factor and a novel inactive mutant rLFm-Y236F. Protein Expr Purif; 2008 May;59(1):25-30
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  • The GST-rLF protein was purified and the GST-tag was then cleaved in a single step by combining both GST-affinity column and treatment with 3C protease.
  • Furthermore, the rLF could induce strong immune response in BALB/c mice and the presence of a specific antiserum could neutralize the cytotoxicity of rLF in vitro.

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  • (PMID = 18276157.001).
  • [ISSN] 1096-0279
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Bacterial; 0 / Bacterial Toxins; 0 / anthrax toxin; EC 3.4.24.- / Metalloendopeptidases
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30. Gill EE, Diaz-Triviño S, Barberà MJ, Silberman JD, Stechmann A, Gaston D, Tamas I, Roger AJ: Novel mitochondrion-related organelles in the anaerobic amoeba Mastigamoeba balamuthi. Mol Microbiol; 2007 Dec;66(6):1306-20
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  • To characterize the evolutionary diversity of these organelles, we conducted an expressed sequence tag (EST) survey on the free-living amoeba Mastigamoeba balamuthi, a relative of the human parasite Entamoeba histolytica.


31. Torreira E, Schoehn G, Fernández Y, Jorba N, Ruigrok RW, Cusack S, Ortín J, Llorca O: Three-dimensional model for the isolated recombinant influenza virus polymerase heterotrimer. Nucleic Acids Res; 2007;35(11):3774-83
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  • We have expressed the virus RNA polymerase complex by co-tranfection of the PB1, PB2 and PA genes in mammalian cells and the heterotrimer was purified by the TAP tag procedure.

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  • (PMID = 17517766.001).
  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / PA protein, influenza viruses; 0 / PB2 protein, Influenzavirus A; 0 / Recombinant Fusion Proteins; 0 / Ribonucleoproteins; 0 / Viral Proteins; 0 / influenza virus polymerase basic protein 1; EC 2.7.7.48 / RNA Replicase
  • [Other-IDs] NLM/ PMC1920261
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32. Miller MR, Nichols PD, Barnes J, Davies NW, Peacock EJ, Carter CG: Regiospecificity profiles of storage and membrane lipids from the gill and muscle tissue of Atlantic salmon (Salmo salar L.) grown at elevated temperature. Lipids; 2006 Sep;41(9):865-76
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  • Further adaptation was indicated by the percentages of SFA, which were significantly (P< 0.05) higher in gill (56%) and white muscle (58%) polar lipid fractions and coincided with lower percentages of n-3, n-6, and total PUFA.
  • The regiospecific profiles indicated a high affinity of DHA to the sn-2 position in both the TAG (61-68%) and polar lipid (35-60%) fractions.

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  • (PMID = 17152924.001).
  • [ISSN] 0024-4201
  • [Journal-full-title] Lipids
  • [ISO-abbreviation] Lipids
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
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33. Akerman M, Mandel-Gutfreund Y: Alternative splicing regulation at tandem 3' splice sites. Nucleic Acids Res; 2006;34(1):23-31
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  • Previous bioinformatics studies based on expressed sequence tag and mRNA data have identified many AS events that are conserved between humans and mice.

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  • (PMID = 16394021.001).
  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
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  • [Other-IDs] NLM/ PMC1325015
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34. Yates SP, Merrill AR: Characterization of oxidized nicotinamide adenine dinucleotide (NAD(+)) analogues using a high-pressure-liquid-chromatography-based NAD(+)-glycohydrolase assay and comparison with fluorescence-based measurements. Anal Biochem; 2005 May 1;340(1):41-51
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  • A high-pressure-liquid-chromatography (HPLC)-based technique was developed to assess the oxidized nicotinamide adenine dinucleotide (NAD(+))-glycohydrolase activity of the catalytic domain of Pseudomonas exotoxin A containing a hexa-His tag.

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  • (PMID = 15802128.001).
  • [ISSN] 0003-2697
  • [Journal-full-title] Analytical biochemistry
  • [ISO-abbreviation] Anal. Biochem.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0U46U6E8UK / NAD; EC 3.2.1.- / Glycoside Hydrolases
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35. Tominaga J, Kamiya N, Doi S, Ichinose H, Maruyama T, Goto M: Design of a specific peptide tag that affords covalent and site-specific enzyme immobilization catalyzed by microbial transglutaminase. Biomacromolecules; 2005 Jul-Aug;6(4):2299-304
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  • [Title] Design of a specific peptide tag that affords covalent and site-specific enzyme immobilization catalyzed by microbial transglutaminase.
  • Using Escherichia coli alkaline phosphatase (AP) as a model, two designed specific peptide tags containing a reactive lysine (Lys) residue with different length Gly-Ser linkers for microbial transglutaminase (MTG) were genetically attached to N- or C-termini.
  • Immobilization by MTG markedly depended on either the position or the length of the peptide tags incorporated to AP, suggesting steric constraint upon enzymatic immobilization.
  • Enzymatically immobilized AP showed comparable catalytic turnover (k(cat)) to the soluble counterpart and comparable operational stability with chemically immobilized AP.
  • These results indicate that attachment of a suitable specific peptide tag to the right position of a target protein is crucial for MTG-mediated formulation of highly active immobilized proteins.

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  • (PMID = 16004475.001).
  • [ISSN] 1525-7797
  • [Journal-full-title] Biomacromolecules
  • [ISO-abbreviation] Biomacromolecules
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Enzymes, Immobilized; 0 / Peptides; 0 / Recombinant Proteins; EC 2.3.2.13 / Transglutaminases; EC 3.1.3.1 / Alkaline Phosphatase
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36. Jiang XZ, Yang MG, Xing XW: [Prokaryotic expression and purification of SPAG4L, a novel human testis gene]. Nan Fang Yi Ke Da Xue Xue Bao; 2010 Sep;30(9):2047-50
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  • After digestion by EcoR I and Hind III, the fragment was subcloned into PQE-30, a prokaryotic expression vector with 6×His tag.
  • The fusion protein SPAG4Lwith 6×his-tag was confirmed by Western blotting.

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  • (PMID = 20855246.001).
  • [ISSN] 1673-4254
  • [Journal-full-title] Nan fang yi ke da xue xue bao = Journal of Southern Medical University
  • [ISO-abbreviation] Nan Fang Yi Ke Da Xue Xue Bao
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Carrier Proteins; 0 / Recombinant Fusion Proteins; 0 / SPAG4 protein, human
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37. Ange-van Heugten KD, Burns R, Verstegen MW, Jansen WL, Ferket PR, van Heugten E: Evaluation of diabetes determinants in woolly monkeys (Lagothrix lagotricha). J Anim Physiol Anim Nutr (Berl); 2007 Dec;91(11-12):481-91
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  • Blood and urine were collected and serum was analysed for fructosamine, glucose, glycated haemoglobin, insulin, triacylglycerides, total cholesterol, high-density lipoprotein cholesterol (HDL-Chol) and low-density lipoprotein cholesterol (LDL-Chol) while urine was tested for glucose concentrations.
  • Serum analyses were within normal ranges (fructosamine (139-242 micromol/l), glucose (2.22-4.78 mmol/l), glycated haemoglobin (3.52-4.73%), insulin (6.2-13.0 microU/ml), triacylglycerides (0.38-3.4 mmol/l), total cholesterol (2.5-5.1 mmol/l), HDL-Chol (0.4-1.6 mmol/l) and LDL-Chol (1.8-3.4 mmol/l)).
  • [MeSH-minor] Animals. Animals, Zoo. Conservation of Natural Resources. Diabetes Mellitus / blood. Diabetes Mellitus / diagnosis. Diabetes Mellitus / urine. Diabetes Mellitus / veterinary. Female. Male

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  • (PMID = 17988352.001).
  • [ISSN] 0931-2439
  • [Journal-full-title] Journal of animal physiology and animal nutrition
  • [ISO-abbreviation] J Anim Physiol Anim Nutr (Berl)
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Germany
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38. Germino M, Sohail H, Germino E, Germino J: A vector for double epitope tagging with a recyclable marker. Yeast; 2006 Jul 30;23(10):763-9
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  • Toward that goal, we have designed a vector that permits the double epitope tagging of a protein at its carboxy terminus.
  • One 'universal' tag, a triple repeat of the HA1 epitope, is fused with every protein to be studied, allowing the composition and stoichiometry of the proteins in a complex to be detected with a single antibody.
  • This 'specific' tag can be used to immunoprecipitate complexes containing that protein of interest.
  • Any epitope to which a specific antibody is available can be used for this second tag.
  • This vector was used to tag 4 proteins involved in ribosome biogenesis-Ytm1, Cic1, Brx1 and Drs1.

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  • [Copyright] Copyright (c) 2006 John Wiley & Sons, Ltd.
  • (PMID = 16862609.001).
  • [ISSN] 0749-503X
  • [Journal-full-title] Yeast (Chichester, England)
  • [ISO-abbreviation] Yeast
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / BRX1 protein, S cerevisiae; 0 / Carrier Proteins; 0 / Cic1 protein, S cerevisiae; 0 / DNA, Fungal; 0 / Epitopes; 0 / Genetic Markers; 0 / Microtubule-Associated Proteins; 0 / RNA-Binding Proteins; 0 / Saccharomyces cerevisiae Proteins; 0 / Ytm1 protein, S cerevisiae
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39. Musacchio T, Toniutti M, Kautz R, Torchilin VP: 1H NMR detection of mobile lipids as a marker for apoptosis: the case of anticancer drug-loaded liposomes and polymeric micelles. Mol Pharm; 2009 Nov-Dec;6(6):1876-82
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  • Cultured cancer cells undergoing apoptosis show an increase in the NMR signal at a chemical shift of 1.3 ppm (-CH2-) corresponding to the so-called "mobile lipids" (ML) originating from the mobile acyl chains in triacylglycerides.

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  • (PMID = 19737025.001).
  • [ISSN] 1543-8392
  • [Journal-full-title] Molecular pharmaceutics
  • [ISO-abbreviation] Mol. Pharm.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / CA128486-02; United States / NCI NIH HHS / CA / R01 CA128486; United States / NCI NIH HHS / CA / R01 CA128486-02
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antineoplastic Agents; 0 / Biomarkers; 0 / Lipids; 0 / Liposomes; 0 / Micelles
  • [Other-IDs] NLM/ NIHMS146904; NLM/ PMC2789870
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40. Ricart-Jané D, Cejudo-Martín P, Peinado-Onsurbe J, López-Tejero MD, Llobera M: Changes in lipoprotein lipase modulate tissue energy supply during stress. J Appl Physiol (1985); 2005 Oct;99(4):1343-51
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  • They modify the flow of energy substrates between tissues, switching the WAT from importer to exporter of free fatty acids and favoring the uptake by muscle of circulating triacylglycerides for energy supply.

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  • (PMID = 15947029.001).
  • [ISSN] 8750-7587
  • [Journal-full-title] Journal of applied physiology (Bethesda, Md. : 1985)
  • [ISO-abbreviation] J. Appl. Physiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / RNA, Messenger; EC 3.1.1.34 / Lipoprotein Lipase
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41. Marszalek JR, Kitidis C, Dirusso CC, Lodish HF: Long-chain acyl-CoA synthetase 6 preferentially promotes DHA metabolism. J Biol Chem; 2005 Mar 18;280(11):10817-26
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  • However, whether Acsl6 overexpression enhanced the amount of PUFA accumulated in the cells or altered the partitioning of any fatty acids into phospholipids (PLs) or triacylglycerides (TAGs) was unknown.
  • Acsl6 overexpression increased the incorporation of [(14)C]-labeled OA, AA, or DHA into PLs and TAGs.
  • These results do not support a role for Acsl6 in the specific targeting of fatty acids into PLs or TAGs.

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  • Gene Ontology. gene/protein/disease-specific - Gene Ontology annotations from this paper .
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  • (PMID = 15655248.001).
  • [ISSN] 0021-9258
  • [Journal-full-title] The Journal of biological chemistry
  • [ISO-abbreviation] J. Biol. Chem.
  • [Language] eng
  • [Grant] United States / NHLBI NIH HHS / HL / P01 HL66105
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Culture Media; 0 / Fatty Acids; 0 / Lipid Bilayers; 0 / Lipids; 0 / RNA, Messenger; 0 / Triglycerides; 137497-17-7 / cyclophilin B; 147336-22-9 / Green Fluorescent Proteins; 25167-62-8 / Docosahexaenoic Acids; 27YG812J1I / Arachidonic Acid; 2UMI9U37CP / Oleic Acid; 459AG36T1B / Dehydroepiandrosterone; EC 5.2.1.- / Cyclophilins; EC 5.2.1.8 / Peptidylprolyl Isomerase; EC 6.2.1.- / Coenzyme A Ligases; EC 6.2.1.3 / long-chain-fatty-acid-CoA ligase
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42. Guion-Almeida ML, Vendramini-Pittoli S, Passos-Bueno MR, Zechi-Ceide RM: Mandibulofacial syndrome with growth and mental retardation, microcephaly, ear anomalies with skin tags, and cleft palate in a mother and her son: autosomal dominant or X-linked syndrome? Am J Med Genet A; 2009 Dec;149A(12):2762-4
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  • [Title] Mandibulofacial syndrome with growth and mental retardation, microcephaly, ear anomalies with skin tags, and cleft palate in a mother and her son: autosomal dominant or X-linked syndrome?
  • [MeSH-major] Cleft Palate / complications. Ear / abnormalities. Genes, X-Linked / genetics. Intellectual Disability / complications. Mandibulofacial Dysostosis / genetics. Microcephaly / complications. Skin Abnormalities / complications


43. Abu-Farha M, Elisma F, Figeys D: Identification of protein-protein interactions by mass spectrometry coupled techniques. Adv Biochem Eng Biotechnol; 2008;110:67-80
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  • Various tags are used to purify protein complexes including tandem affinity purification.
  • The FLAG tag is another commonly used tag which is a small tag that tends not to interfere with the protein function.

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  • (PMID = 18227982.001).
  • [ISSN] 1616-8542
  • [Journal-full-title] Advances in biochemical engineering/biotechnology
  • [ISO-abbreviation] Adv. Biochem. Eng. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Affinity Labels; 0 / Proteins
  • [Number-of-references] 50
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44. Maillot F, Baulieu F, Guilloteau D, Boirie Y, Garrigue MA, Hoinard C, Couet C: Gut emptying affects dietary fat contribution to postprandial lipemia following sequential meals in healthy subjects. Nutrition; 2008 Jul-Aug;24(7-8):682-8
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  • OBJECTIVE: The present study examined the kinetic of plasma triacylglycerol (TAG) and gut emptying after sequential ingestion of breakfast and lunch, and the contribution of dietary fat ingested at breakfast to subsequent TAG after lunch.
  • Plasma TAG and chylomicron-TAG (CM-TAG) concentrations and [1-(13)C] palmitate enrichment were sequentially measured.
  • RESULTS: An early rise in plasma and CM-TAG was observed after lunch ingestion.
  • After breakfast, [1-(13)C] palmitate enrichment was maximal 150 and 210 min in plasma TAG and CM-TAG, respectively, decreased thereafter, and increased rapidly (50 min for plasma TAG and 30 min for CM-TAG) after lunch ingestion.

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  • (PMID = 18486449.001).
  • [ISSN] 0899-9007
  • [Journal-full-title] Nutrition (Burbank, Los Angeles County, Calif.)
  • [ISO-abbreviation] Nutrition
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Carbon Isotopes; 0 / Dietary Fats; 0 / Iodine Radioisotopes; 0 / Lipids; 0 / Triglycerides
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45. Verma SK, Bansal I, Vedi S, Saxena JK, Katoch VM, Bhattacharya SM: Molecular cloning, purification and characterisation of myosin of human lymphatic filarial parasite Brugia malayi. Parasitol Res; 2008 Feb;102(3):481-9
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  • The purified protein was recognised in western blot with anti-His tag antibody as also with the antibodies present in the sera of human W. bancrofti patients of all categories and infected/immunized rodent serum demonstrating its functional role.
  • The present findings demonstrate the usefulness of B. malayi recombinant myosin as vaccine candidate against human lymphatic filariasis.

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  • (PMID = 18064491.001).
  • [ISSN] 0932-0113
  • [Journal-full-title] Parasitology research
  • [ISO-abbreviation] Parasitol. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antibodies, Helminth; 0 / Antigens, Helminth; 0 / DNA, Complementary; 0 / DNA, Helminth; 0 / Vaccines; EC 3.6.4.1 / Myosins
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46. Xiong Y, Bernardi D, Bratton S, Ward MD, Battaglia E, Finel M, Drake RR, Radominska-Pandya A: Phenylalanine 90 and 93 are localized within the phenol binding site of human UDP-glucuronosyltransferase 1A10 as determined by photoaffinity labeling, mass spectrometry, and site-directed mutagenesis. Biochemistry; 2006 Feb 21;45(7):2322-32
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  • Analysis of recombinant His-tag UGTs from the 1A family for their ability to glucuronidate p-nitrophenol (pNP) and 4-methylumbelliferone (4-MU) revealed that UGT1A10 shows high activity toward phenols and phenol derivatives.

  • Hazardous Substances Data Bank. 4-NITROPHENOL .
  • Hazardous Substances Data Bank. PHENOL .
  • Hazardous Substances Data Bank. SODIUM PHENOLATE .
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  • (PMID = 16475821.001).
  • [ISSN] 0006-2960
  • [Journal-full-title] Biochemistry
  • [ISO-abbreviation] Biochemistry
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / CA908028; United States / NIDDK NIH HHS / DK / DK49715; United States / NIDDK NIH HHS / DK / DK51971
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Azides; 0 / Nitrophenols; 0 / Photoaffinity Labels; 0 / Recombinant Proteins; 0 / Salicylates; 339NCG44TV / Phenol; 3T5NG4Q468 / Hymecromone; 47E5O17Y3R / Phenylalanine; 66761-27-1 / 4-azidosalicylic acid; EC 2.4.1.- / bilirubin uridine-diphosphoglucuronosyl transferase 1A10; EC 2.4.1.17 / Glucuronosyltransferase; Y92ZL45L4R / 4-nitrophenol
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47. Redaelli L, Zolezzi F, Nardese V, Bellanti B, Wanke V, Carettoni D: A platform for high-throughput expression of recombinant human enzymes secreted by insect cells. J Biotechnol; 2005 Oct 17;120(1):59-71
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  • To contribute to the assignment of a physiological role to the vast number of coding genes with unknown function, we have undertaken a program to clone, express, purify and determine the catalytic activity of those enzymes predicted to enter the secretory pathway, focusing our efforts on human peptidases.
  • Our strategy to promote high-throughput expression and purification of recombinant proteins secreted by insect cells relies on the expression of the target enzymes with their native leader sequences and on the carboxyl-terminal fusion with a poly-histidine tag.

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  • (PMID = 16043252.001).
  • [ISSN] 0168-1656
  • [Journal-full-title] Journal of biotechnology
  • [ISO-abbreviation] J. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Enzymes; 0 / Recombinant Proteins
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48. Na S, Jeong J, Park H, Lee KJ, Paek E: Unrestrictive identification of multiple post-translational modifications from tandem mass spectrometry using an error-tolerant algorithm based on an extended sequence tag approach. Mol Cell Proteomics; 2008 Dec;7(12):2452-63
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  • [Title] Unrestrictive identification of multiple post-translational modifications from tandem mass spectrometry using an error-tolerant algorithm based on an extended sequence tag approach.
  • MOD(i) introduces the notion of a tag chain, a combination structure made from multiple sequence tags, that effectively localizes modified regions within a spectrum and overcomes de novo sequencing errors common in tag-based approaches.

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  • (PMID = 18701446.001).
  • [ISSN] 1535-9484
  • [Journal-full-title] Molecular & cellular proteomics : MCP
  • [ISO-abbreviation] Mol. Cell Proteomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Eye Proteins; 0 / Peptides; EC 1.2.1.12 / Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating)
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49. Kędzierski W, Kapica M: Plasma concentration of leptin and ghrelin in Standardbred foals as related to the age, sex, exercise and training. Animal; 2008 Apr;2(4):582-7
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  • A positive correlation between the plasma concentrations of leptin and triacylglycerides measured just after exercise was detected (r = 0.65).

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  • (PMID = 22443573.001).
  • [ISSN] 1751-7311
  • [Journal-full-title] Animal : an international journal of animal bioscience
  • [ISO-abbreviation] Animal
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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50. Choi YH, Choi HJ, Kim D, Uhm KN, Kim HK: Asymmetric synthesis of (S)-3-chloro-1-phenyl-1-propanol using Saccharomyces cerevisiae reductase with high enantioselectivity. Appl Microbiol Biotechnol; 2010 Jun;87(1):185-93
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  • The yeast reductase YOL151W (GenBank locus tag) exhibited the highest level of activity and exclusively generated the (S)-alcohol.

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  • (PMID = 20111861.001).
  • [ISSN] 1432-0614
  • [Journal-full-title] Applied microbiology and biotechnology
  • [ISO-abbreviation] Appl. Microbiol. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / 3-chloro-1-phenylpropanol; 0 / Hydrocarbons, Chlorinated; 0 / Propanols; 0 / Saccharomyces cerevisiae Proteins; 53-59-8 / NADP; EC 1.- / Oxidoreductases; EC 1.1.1.283 / GRE2 protein, S cerevisiae
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51. Vandana S, Bhatla SC: Co-localization of putative calcium channels (phenylalkylamine-binding sites) on oil bodies in protoplasts from dark-grown sunflower seedling cotyledons. Plant Signal Behav; 2009 Jul;4(7):604-9
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  • Oil bodies are spherical entities containing a triacylglycerol (TAG) matrix encased by a phospholipid monolayer, which is stabilized by oil body-specific proteins, principally oleosins.

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  • (PMID = 19820351.001).
  • [ISSN] 1559-2324
  • [Journal-full-title] Plant signaling & behavior
  • [ISO-abbreviation] Plant Signal Behav
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Calcium Channels, L-Type; 0 / Nitriles; 0 / Phenethylamines; 0 / Plant Oils; 0 / Plant Proteins; 139972-87-5 / 5-(3-(3-(4,4-difluoro-5,7-dimethyl-3,4-diaza-4-bora-indacen-3-yl)propionamido)phenethyl-N-methylamino)-2-isopropyl-2-(3,4,5-trimethoxyphenyl)valeronitrile; SY7Q814VUP / Calcium
  • [Other-IDs] NLM/ PMC2710551
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52. Schrider DR, Hahn MW: Lower linkage disequilibrium at CNVs is due to both recurrent mutation and transposing duplications. Mol Biol Evol; 2010 Jan;27(1):103-11
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  • In support of our hypothesis, we find that duplications are significantly less likely than deletions to have a "tag" SNP, even after controlling for CNV length, allele frequency, and availability of appropriate flanking SNPs.

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  • [ISSN] 1537-1719
  • [Journal-full-title] Molecular biology and evolution
  • [ISO-abbreviation] Mol. Biol. Evol.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM076643; United States / NIGMS NIH HHS / GM / R01-GM076643A
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Other-IDs] NLM/ PMC2877554
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53. Plant KP, LaPatra SE, Cain KD: Vaccination of rainbow trout, Oncorhynchus mykiss (Walbaum), with recombinant and DNA vaccines produced to Flavobacterium psychrophilum heat shock proteins 60 and 70. J Fish Dis; 2009 Jun;32(6):521-34
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  • Some cross reactivity occurred, which may have been due to the V5 tag common to both proteins.

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  • (PMID = 19460087.001).
  • [ISSN] 1365-2761
  • [Journal-full-title] Journal of fish diseases
  • [ISO-abbreviation] J. Fish Dis.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies, Bacterial; 0 / Chaperonin 60; 0 / DNA Primers; 0 / HSP70 Heat-Shock Proteins; 0 / Vaccines, DNA
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54. Elias DA, Mukhopadhyay A, Joachimiak MP, Drury EC, Redding AM, Yen HC, Fields MW, Hazen TC, Arkin AP, Keasling JD, Wall JD: Expression profiling of hypothetical genes in Desulfovibrio vulgaris leads to improved functional annotation. Nucleic Acids Res; 2009 May;37(9):2926-39
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  • To accomplish this, expression profiles of 1234 HyP and conserved genes were used from transcriptomic datasets of 11 environmental stresses, complemented with shotgun LC-MS/MS and AMT tag proteomic data.

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  • (PMID = 19293273.001).
  • [ISSN] 1362-4962
  • [Journal-full-title] Nucleic acids research
  • [ISO-abbreviation] Nucleic Acids Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] England
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  • [Other-IDs] NLM/ PMC2685097
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55. Kim DY, Kaiser TJ, Horlen K, Keith ML, Taylor LP, Jolie R, Calvert JG, Rowland RR: Insertion and deletion in a non-essential region of the nonstructural protein 2 (nsp2) of porcine reproductive and respiratory syndrome (PRRS) virus: effects on virulence and immunogenicity. Virus Genes; 2009 Feb;38(1):118-28
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  • Developing a vaccine that can differentiate infected and vaccinated animals (DIVA) is a new challenge in the design of a vaccine for porcine reproductive and respiratory syndrome virus (PRRSV).
  • The deleted region was replaced with a peptide tag encoding eight amino acids.

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  • (PMID = 19048364.001).
  • [ISSN] 0920-8569
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  • [ISO-abbreviation] Virus Genes
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
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56. Cox J, Hubner NC, Mann M: How much peptide sequence information is contained in ion trap tandem mass spectra? J Am Soc Mass Spectrom; 2008 Dec;19(12):1813-20
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  • These sequences are almost always unique in the human proteome, even without using any precursor or peptide sequence tag information.

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  • (PMID = 18757209.001).
  • [ISSN] 1044-0305
  • [Journal-full-title] Journal of the American Society for Mass Spectrometry
  • [ISO-abbreviation] J. Am. Soc. Mass Spectrom.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Ions; 0 / Peptides
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57. Gillies AR, Hsii JF, Oak S, Wood DW: Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags. Biotechnol Bioeng; 2008 Oct 1;101(2):229-40
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  • [Title] Rapid cloning and purification of proteins: gateway vectors for protein purification by self-cleaving tags.
  • We have combined Invitrogen's Gateway cloning technology with self-cleaving purification tags to generate a new system for rapid production of recombinant protein products.
  • We have incorporated this intein into a set of Gateway vectors, which include self-cleaving elastin-like polypeptide (ELP), chitin binding domain (CBD), phasin (polyhydroxybutyrate-binding), or maltose binding domain (MBD) tags.
  • The modular design of the Gateway system and intein purification method suggests that any tag and promoter can be trivially added to this system for the development of additional expression vectors.

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  • (PMID = 18727029.001).
  • [ISSN] 1097-0290
  • [Journal-full-title] Biotechnology and bioengineering
  • [ISO-abbreviation] Biotechnol. Bioeng.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Escherichia coli Proteins; 0 / Recombinant Proteins; EC 1.11.1.6 / Catalase; EC 1.11.1.6 / katG protein, E coli; EC 3.5.2.6 / beta-Lactamases; EC 3.5.2.6 / beta-lactamase TEM-1
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58. Du M, Zhou F: Postcolumn renewal of sensor surfaces for high-performance liquid chromatography-surface plasmon resonance detection. Anal Chem; 2008 Jun 1;80(11):4225-30
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  • The renewable SPR detection of continuous separations is reproducible and versatile and does not require the separated proteins to contain chromophores or to be prelabeled with a tag (e.g., a redox-active or fluorescent molecule).

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  • (PMID = 18457411.001).
  • [ISSN] 1520-6882
  • [Journal-full-title] Analytical chemistry
  • [ISO-abbreviation] Anal. Chem.
  • [Language] eng
  • [Grant] United States / NIGMS NIH HHS / GM / GM-08101; United States / NIMHD NIH HHS / MD / P20-MD001824-01
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Proteins
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59. Evans DM, Barrett JC, Cardon LR: To what extent do scans of non-synonymous SNPs complement denser genome-wide association studies? Eur J Hum Genet; 2008 Jun;16(6):718-23
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  • We demonstrate that commercially available genome-wide panels already tag the majority of common non-synonymous variants on the NS-12 BeadChip, indicating that with respect to capturing common non-synonymous variation, information from the NS-12 BeadChip is largely redundant.

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  • (PMID = 18197186.001).
  • [ISSN] 1018-4813
  • [Journal-full-title] European journal of human genetics : EJHG
  • [ISO-abbreviation] Eur. J. Hum. Genet.
  • [Language] eng
  • [Grant] United Kingdom / Medical Research Council / / G0600705; United States / NEI NIH HHS / EY / EY 126562; United Kingdom / Wellcome Trust / /
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] England
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60. Xu X, Jin F, Yu X, Ji S, Wang J, Cheng H, Wang C, Zhang W: Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein Expr Purif; 2007 Jun;53(2):293-301
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  • [Title] Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli.
  • An enterokinase cleavage site was introduced between the 6xHis-tag and Mdmcec to facilitate final release of the recombinant Mdmcec.

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  • (PMID = 17300953.001).
  • [ISSN] 1046-5928
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antimicrobial Cationic Peptides; 0 / DNA Primers; 0 / Insect Proteins; 0 / Recombinant Fusion Proteins; 52500-60-4 / Thioredoxins
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61. Song SH, Dick B, Zirak P, Penzkofer A, Schiereis T, Hegemann P: Absorption and emission spectroscopic characterisation of combined wildtype LOV1-LOV2 domain of phot from Chlamydomonas reinhardtii. J Photochem Photobiol B; 2005 Oct 3;81(1):55-65
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  • A LOV1/2-MBP fusion protein (MBP=maltose binding protein) and LOV1/2 with a His-tag at the C-terminus (LOV1/2-His) expressed in an Escherichia coli strain are investigated.

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  • (PMID = 16111889.001).
  • [ISSN] 1011-1344
  • [Journal-full-title] Journal of photochemistry and photobiology. B, Biology
  • [ISO-abbreviation] J. Photochem. Photobiol. B, Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / Carrier Proteins; 0 / Cryptochromes; 0 / Flavoproteins; 0 / Maltose-Binding Proteins; 0 / Photoreceptors, Microbial; 0 / Recombinant Fusion Proteins; 0 / flavin mononucleotide-C(4a)-cysteine adduct; 26062-48-6 / polyhistidine; 4QD397987E / Histidine; 7N464URE7E / Flavin Mononucleotide; K848JZ4886 / Cysteine; TLM2976OFR / Riboflavin
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62. Fuwa H, Hiromoto K, Takahashi Y, Yokoshima S, Kan T, Fukuyama T, Iwatsubo T, Tomita T, Natsugari H: Synthesis of biotinylated photoaffinity probes based on arylsulfonamide gamma-secretase inhibitors. Bioorg Med Chem Lett; 2006 Aug 15;16(16):4184-9
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  • Design, synthesis, and biological evaluation of multifunctional molecular probes harboring a benzophenone photophore as a cross-linking group and a biotin tag are also reported.

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  • (PMID = 16766183.001).
  • [ISSN] 0960-894X
  • [Journal-full-title] Bioorganic & medicinal chemistry letters
  • [ISO-abbreviation] Bioorg. Med. Chem. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / 4-(2-((1R)-1-(((4-chlorophenyl)sulfonyl)-2,5-difluoroanilino)ethyl)-5-fluorophenyl)butanoic acid; 0 / Arylsulfonates; 0 / Butyrates; 0 / Cross-Linking Reagents; 0 / Enzyme Inhibitors; 0 / Hydrocarbons, Halogenated; 0 / Photoaffinity Labels; EC 3.4.- / Amyloid Precursor Protein Secretases; EC 3.4.- / Endopeptidases
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63. Miller DJ, Ball EE: The gene complement of the ancestral bilaterian - was Urbilateria a monster? J Biol; 2009;8(10):89
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  • Expressed sequence tag analyses of the annelid Pomatoceros lamarckii, recently published in BMC Evolutionary Biology, are consistent with less extensive gene loss in the Lophotrochozoa than in the Ecdysozoa, but it would be premature to generalize about patterns of gene loss on the basis of the limited data available.

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  • [Cites] Curr Biol. 2003 Dec 16;13(24):2190-5 [14680636.001]
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  • (PMID = 19939290.001).
  • [ISSN] 1475-4924
  • [Journal-full-title] Journal of biology
  • [ISO-abbreviation] J. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Number-of-references] 10
  • [Other-IDs] NLM/ PMC2790832
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64. Naziroglu M, Brandsch C: Dietary hydrogenated soybean oil affects lipid and vitamin E metabolism in rats. J Nutr Sci Vitaminol (Tokyo); 2006 Apr;52(2):83-8
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  • Fatty acids containing stearic acid, which are found in hydrogenated fat, may have a detrimental effect on the cholesterol and triacylglycerol (TAG) content of plasma lipoproteins, and on the absorption of fatty acids and fat-soluble vitamins.
  • Plasma total cholesterol, VLDL- and LDL-cholesterol, lipid peroxidation and daily excretion of the TAG and cholesterol in feces were higher in the HSO than in the control group.
  • TAG values in plasma and liver, and HDL-cholesterol levels in plasma were lower in the HSO than in the control group.
  • The results of this study provide new evidence concerning the effect of dietary hydrogenated fat on lipid, TAG and vitamin E status, which are important for maintenance of good health.

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  • (PMID = 16802687.001).
  • [ISSN] 0301-4800
  • [Journal-full-title] Journal of nutritional science and vitaminology
  • [ISO-abbreviation] J. Nutr. Sci. Vitaminol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Japan
  • [Chemical-registry-number] 0 / Dietary Fats; 0 / Lipoproteins; 0 / Phospholipids; 0 / Triglycerides; 11103-57-4 / Vitamin A; 1406-18-4 / Vitamin E; 8001-22-7 / Soybean Oil; 97C5T2UQ7J / Cholesterol
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65. Shoja V, Zhang L: A roadmap of tandemly arrayed genes in the genomes of human, mouse, and rat. Mol Biol Evol; 2006 Nov;23(11):2134-41
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  • Tandemly arrayed genes (TAGs) play an important functional and physiological role in the genome.
  • Most previous studies have focused on individual TAG families in a few species, yet a broad characterization of TAGs is not available.
  • Here we identified all TAGs in the genomes of humans, mouse, and rat and performed a comprehensive analysis of TAG distribution, TAG sizes, TAG orientations and intergenic distances, and TAG functions.
  • TAGs account for about 14-17% of all genes in the genome and nearly one-third of all duplicated genes, highlighting the predominant role that tandem duplication plays in gene duplication.
  • For all species, TAG distribution is highly heterogeneous along chromosomes and some chromosomes are enriched with TAG forests, whereas others are enriched with TAG deserts.
  • The majority of TAGs are of size 2 for all genomes, similar to the previous findings in Caenorhabditis elegans, Arabidopsis thaliana, and Oryza sativa, suggesting that it is a rather general phenomenon in eukaryotes.
  • The comparison with the genome patterns shows that TAG members have a significantly higher proportion of parallel gene orientation in all species, corroborating Graham's claim that parallel orientation is the preferred form of orientation in TAGs.
  • Moreover, TAG members with parallel orientation tend to be closer to each other than all neighboring genes in the genome with parallel orientation.
  • The analyses of Gene Ontology function indicate that genes with receptor or binding activities are significantly overrepresented by TAGs.
  • Computer simulation reveals that random gene rearrangements have little effect on the statistics of TAGs for all genomes.
  • Finally, the average proportion of TAGs shows a trend of increase with the increase of family sizes, although the correlation between TAG proportions in individual families and family sizes is not significant.

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  • (PMID = 16901985.001).
  • [ISSN] 0737-4038
  • [Journal-full-title] Molecular biology and evolution
  • [ISO-abbreviation] Mol. Biol. Evol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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66. Liu YC, Rieben N, Iversen L, Sørensen BS, Park J, Nygård J, Martinez KL: Specific and reversible immobilization of histidine-tagged proteins on functionalized silicon nanowires. Nanotechnology; 2010 Jun 18;21(24):245105
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  • In this study, we show that SiNWs can be chemically functionalized with Ni:NTA motifs, suitable for the specific immobilization of proteins via a short polyhistidine tag (His-tag) at close proximity to the SiNW surface.

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  • Hazardous Substances Data Bank. NITRILOTRIACETIC ACID .
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  • (PMID = 20498527.001).
  • [ISSN] 1361-6528
  • [Journal-full-title] Nanotechnology
  • [ISO-abbreviation] Nanotechnology
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Immobilized Proteins; 0 / Proteins; 4QD397987E / Histidine; 7OV03QG267 / Nickel; KA90006V9D / Nitrilotriacetic Acid; Z4152N8IUI / Silicon
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67. Meyer M, Stenzel U, Hofreiter M: Parallel tagged sequencing on the 454 platform. Nat Protoc; 2008;3(2):267-78
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  • The method relies on attaching sample-specific barcoding adapters, which include sequence tags and a restriction site, to blunt-end repaired DNA samples by ligation and strand-displacement.
  • After pooling multiple barcoded samples, molecules without sequence tags are effectively excluded from sequencing by dephosphorylation and restriction digestion, and using the tag sequences, the source of each DNA sequence can be traced.

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  • (PMID = 18274529.001).
  • [ISSN] 1750-2799
  • [Journal-full-title] Nature protocols
  • [ISO-abbreviation] Nat Protoc
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / DNA, Mitochondrial
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68. Ollis AA, Manning M, Held KG, Postle K: Cytoplasmic membrane protonmotive force energizes periplasmic interactions between ExbD and TonB. Mol Microbiol; 2009 Aug;73(3):466-81
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  • Analysis of T7-tagged ExbD with anti-ExbD antibodies revealed that a T7 tag was responsible both for our previous failure to detect T7-ExbD-ExbB and T7-ExbD-TonB formaldehyde-linked complexes and for the concomitant artefactual appearance of T7-ExbD trimers.

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  • (PMID = 19627500.001).
  • [ISSN] 1365-2958
  • [Journal-full-title] Molecular microbiology
  • [ISO-abbreviation] Mol. Microbiol.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / GM042146-19; United States / NIGMS NIH HHS / GM / R01 GM042146; United States / NIGMS NIH HHS / GM / R01 GM042146-19
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Cross-Linking Reagents; 0 / Escherichia coli Proteins; 0 / ExbB protein, E coli; 0 / Membrane Proteins; 0 / tonB protein, E coli; 123424-75-9 / exbD protein, E coli; 1HG84L3525 / Formaldehyde
  • [Other-IDs] NLM/ NIHMS134992; NLM/ PMC2729267
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69. Lancioni GE, O'Reilly MF, Singh NN, Sigafoos J, Didden R, Oliva D, Montironi G: Persons with multiple disabilities and minimal motor behavior using small forehead movements and new microswitch technology to control environmental stimuli. Percept Mot Skills; 2007 Jun;104(3 Pt 1):870-8
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  • In the present study, a small movement of the forehead skin was selected as the response for two participants (ages 6.5 and 14.2 years) with profound multiple disabilities.
  • The microswitch technology included (a) an optic sensor, i.e., barcode reader, (b) a small tag with horizontal bars attached to the participants' forehead, and (c) an electronic control system which activated stimuli in relation to response occurrence.
  • Movement of the forehead skin shifted up or down the tag with bars and this shifting, if greater than a preset limit and therefore recorded as a response, led to the activation of the control system.
  • [MeSH-major] Communication Aids for Disabled. Disabled Persons / education. Disabled Persons / rehabilitation. Electronics, Medical / instrumentation. Forehead / physiology. Movement / physiology. Skin Physiological Phenomena

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  • (PMID = 17688142.001).
  • [ISSN] 0031-5125
  • [Journal-full-title] Perceptual and motor skills
  • [ISO-abbreviation] Percept Mot Skills
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] United States
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70. Ahn YH, Ji ES, Lee JY, Cho K, Yoo JS: Arginine-mimic labeling with guanidinoethanethiol to increase mass sensitivity of lysine-terminated phosphopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Rapid Commun Mass Spectrom; 2007;21(14):2204-10
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  • Here, we report a new method for analyzing phosphopeptides in which phosphopeptides are labeled with a novel chemical tag, guanidinoethanethiol (GET), by a beta-elimination/Michael addition before MS analysis.
  • GET labeling of lysine-terminated phosphopeptides improved sensitivity up to 22 times compared to that of the corresponding aminoethanethiol (AET) labeling, in which AET was used as a labeling tag containing an amino group instead of the guanidine group.

  • Hazardous Substances Data Bank. GUANIDINE .
  • Hazardous Substances Data Bank. L-Lysine .
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  • [Copyright] Copyright 2007 John Wiley & Sons, Ltd.
  • (PMID = 17569100.001).
  • [ISSN] 0951-4198
  • [Journal-full-title] Rapid communications in mass spectrometry : RCM
  • [ISO-abbreviation] Rapid Commun. Mass Spectrom.
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Phosphopeptides; 0 / Sulfhydryl Compounds; 94ZLA3W45F / Arginine; JU58VJ6Y3B / Guanidine; K3Z4F929H6 / Lysine; M439R54A1D / ethanethiol
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71. Tan JG, Zhou LJ, Feng M, Feng HL: [Ultrastructural study of a self-etching adhesive to sclerotic dentin in non-carious cervical lesions]. Zhonghua Kou Qiang Yi Xue Za Zhi; 2005 May;40(3):230-2
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  • [Title] [Ultrastructural study of a self-etching adhesive to sclerotic dentin in non-carious cervical lesions].
  • OBJECTIVE: To examine, in vitro study, the ultrastructure of resin-infiltrated sclerotic dentine following the application of a two-step self-etching dentin adhesive.
  • By SEM examination, the morphological change of the dentin surface treated by Contax Primer, and the hybrid layer and resin tag in the dentin-resin interface were studied.
  • Both hybrid layer and resin tag were observed in sclerotic dentin.
  • The hybrid layer was almost similar to that in the sound dentin, but the resin tags were shorter than those in controls.
  • Based on the present ultrastructural features of hybrid layer and resin tags, the Contax, a self-etching dentin adhesive with a low pH value primer, may have some bonding efficacy to sclerotic dentin.

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  • (PMID = 15938888.001).
  • [ISSN] 1002-0098
  • [Journal-full-title] Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology
  • [ISO-abbreviation] Zhonghua Kou Qiang Yi Xue Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Dentin-Bonding Agents
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72. Moran-Mirabal JM, Corgie SC, Bolewski JC, Smith HM, Cipriany BR, Craighead HG, Walker LP: Labeling and purification of cellulose-binding proteins for high resolution fluorescence applications. Anal Chem; 2009 Oct 1;81(19):7981-7
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  • To have meaningful interpretations of results from the study of labeled enzymes, it is then necessary to reduce the variability in physical, chemical, and biological characteristics of the labeled products.
  • In this paper, a solid phase labeling protocol is described as an advantageous alternative to free solution labeling of cellulose-binding proteins and is applied to tag cellulases with three different fluorophores.

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  • (PMID = 19728729.001).
  • [ISSN] 1520-6882
  • [Journal-full-title] Analytical chemistry
  • [ISO-abbreviation] Anal. Chem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Fluorescent Dyes; EC 3.2.1.- / Cellulases
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73. Krynetskiy E, McDonnell P: Building individualized medicine: prevention of adverse reactions to warfarin therapy. J Pharmacol Exp Ther; 2007 Aug;322(2):427-34
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  • Discrimination between haplotypes associated with warfarin dose phenotypes can be achieved by a limited set of informative polymorphisms (tag SNPs).
  • The use of tag SNPs in pharmacogenomic analysis provides a promising tool for dissecting polygenic traits of drug response.

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  • (PMID = 17496169.001).
  • [ISSN] 0022-3565
  • [Journal-full-title] The Journal of pharmacology and experimental therapeutics
  • [ISO-abbreviation] J. Pharmacol. Exp. Ther.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Anticoagulants; 0 / Orosomucoid; 0 / Protein C; 5Q7ZVV76EI / Warfarin; EC 1.- / Mixed Function Oxygenases; EC 1.1.4.1 / Vitamin K Epoxide Reductases; EC 1.14.13.- / CYP2C9 protein, human; EC 1.14.13.- / Cytochrome P-450 CYP2C9; EC 1.14.14.1 / Aryl Hydrocarbon Hydroxylases; EC 1.17.4.4 / VKORC1 protein, human; EC 3.3.2.- / Epoxide Hydrolases; EC 6.4.- / Carbon-Carbon Ligases; EC 6.4.- / glutamyl carboxylase
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74. Zeng Y, Yokohira M, Takeuchi H, Saoo K, Yamakawa K, Matsuda Y, Hosokawa K, Li JQ, Ikeda M, Imaida K: Lack of significant modifying effect of arctiin on prostate carcinogenesis in probasin/SV40 T antigen transgenic rats. Cancer Lett; 2005 May 26;222(2):145-51
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  • Modifying effects of arctiin on prostate carcinogenesis in probasin/SV 40 T antigen (Tag) transgenic (TG) rats were examined.
  • These results indicated that arctiin might not exert significant modifying effect on prostate carcinogenesis in SV 40 Tag TG rats at least under the present experiment.

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  • (PMID = 15863263.001).
  • [ISSN] 0304-3835
  • [Journal-full-title] Cancer letters
  • [ISO-abbreviation] Cancer Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Androgen-Binding Protein; 0 / Antigens, Polyomavirus Transforming; 0 / Drugs, Chinese Herbal; 0 / Furans; 0 / Glucosides; 0 / probasin; TM5RQ949K7 / arctiin
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75. Kinoshita E, Kinoshita-Kikuta E, Takiyama K, Koike T: Phosphate-binding tag, a new tool to visualize phosphorylated proteins. Mol Cell Proteomics; 2006 Apr;5(4):749-57
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  • [Title] Phosphate-binding tag, a new tool to visualize phosphorylated proteins.
  • Zn(2+) or Mn(2+)) complexes as novel phosphate-binding tag (Phos-tag) molecules.
  • Both Zn(2+)- and Mn(2+)-Phos-tag molecules preferentially capture phosphomonoester dianions bound to Ser, Thr, and Tyr residues.
  • One method is based on an ECL system using biotin-pendant Zn(2+)-Phos-tag and horseradish peroxidase-conjugated streptavidin.
  • Another method is based on the mobility shift of phosphorylated proteins in SDS-PAGE with polyacrylamide-bound Mn(2+)-Phos-tag.
  • We demonstrate the kinase and phosphatase assays by phosphate affinity electrophoresis (Mn(2+)-Phos-tag SDS-PAGE).

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  • (PMID = 16340016.001).
  • [ISSN] 1535-9476
  • [Journal-full-title] Molecular & cellular proteomics : MCP
  • [ISO-abbreviation] Mol. Cell Proteomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Proteins
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76. He J, Westbrooks K, Zelikovsky A: Linear reduction method for predictive and informative tag SNP selection. Int J Bioinform Res Appl; 2005;1(3):249-60
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  • [Title] Linear reduction method for predictive and informative tag SNP selection.
  • Therefore, it is desirable to reduce the number of SNPs that should be sequenced to a small number of informative representatives called tag SNPs.
  • In this paper, we propose a new linear algebra-based method for selecting and using tag SNPs.
  • We measure the quality of our tag SNP selection algorithm by comparing actual SNPs with SNPs predicted from selected linearly independent tag SNPs.

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  • (PMID = 18048134.001).
  • [ISSN] 1744-5485
  • [Journal-full-title] International journal of bioinformatics research and applications
  • [ISO-abbreviation] Int J Bioinform Res Appl
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Switzerland
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77. Lee MR, Jung DW, Williams D, Shin I: Efficient solid-phase synthesis of trifunctional probes and their application to the detection of carbohydrate-binding proteins. Org Lett; 2005 Nov 24;7(24):5477-80
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  • [structure: see text] An efficient solid-phase synthesis of trifunctional probes containing a photoreactive group, a reporter tag, and a carbohydrate ligand was developed.

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  • (PMID = 16288535.001).
  • [ISSN] 1523-7060
  • [Journal-full-title] Organic letters
  • [ISO-abbreviation] Org. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Carbohydrates; 0 / Lectins; 0 / Ligands; 0 / Molecular Probes
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78. Bourquin C, von der Borch P, Zoglmeier C, Anz D, Sandholzer N, Suhartha N, Wurzenberger C, Denzel A, Kammerer R, Zimmermann W, Endres S: Efficient eradication of subcutaneous but not of autochthonous gastric tumors by adoptive T cell transfer in an SV40 T antigen mouse model. J Immunol; 2010 Aug 15;185(4):2580-8
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  • We investigated the efficacy of immunotherapy in an autochthonous model of gastric cancer, the CEA424-SV40 T Ag (TAg) transgenic mice.
  • In contrast, neither s.c. nor autochthonous tumors responded to vaccination in CEA424-SV40 TAg mice, indicating tolerance to the SV40 TAg.
  • To examine whether tumors in these mice were principally accessible to immunotherapy, splenocytes from immune wild-type mice were adoptively transferred into CEA424-SV40 TAg transgenic mice.

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  • (PMID = 20644173.001).
  • [ISSN] 1550-6606
  • [Journal-full-title] Journal of immunology (Baltimore, Md. : 1950)
  • [ISO-abbreviation] J. Immunol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Polyomavirus Transforming; 0 / Cancer Vaccines; 0 / Carcinoembryonic Antigen; 0 / Oligonucleotides; 0 / Recombinant Fusion Proteins
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79. Sims DW, Queiroz N, Humphries NE, Lima FP, Hays GC: Long-term GPS tracking of ocean sunfish Mola mola offers a new direction in fish monitoring. PLoS One; 2009;4(10):e7351
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  • Up to now, such fish have been tracked remotely using two principal methods: direct positioning of transmitters by Argos polar-orbiting satellites, and satellite relay of tag-derived light-level data for post hoc track reconstruction.
  • Here we use a fast-acquisition global positioning system (Fastloc GPS) tag with remote data retrieval to track long-term movements, in near real time and position accuracy of <70 m, of the world's largest bony fish, the ocean sunfish Mola mola.
  • These 'stopovers' during long-distance movement appear consistent with finding and exploiting food patches.
  • This demonstrates the feasibility of GPS tagging to provide tracks of unparalleled accuracy for monitoring movements of large pelagic fish, and with nearly four times as many locations obtained by the GPS tag than by a conventional Argos transmitter.

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  • [Cites] Biol Lett. 2009 Jun 23;5(3):289-92 [19324624.001]
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  • (PMID = 19816576.001).
  • [ISSN] 1932-6203
  • [Journal-full-title] PloS one
  • [ISO-abbreviation] PLoS ONE
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Other-IDs] NLM/ PMC2754528
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80. Pöhner C, Hilbrig F, Jérôme V, Freitag R: Design and characterization of stimuli-responsive FLAG-tag analogues and the illumination-induced modulation of their interaction with antibody 4E11. Biotechnol Prog; 2006 Jul-Aug;22(4):1170-8
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  • [Title] Design and characterization of stimuli-responsive FLAG-tag analogues and the illumination-induced modulation of their interaction with antibody 4E11.
  • Photopeptides that contained the intact sequences responsible for binding of the FLAG tag to the antibody, namely, the DYK motive at the N-terminus, showed binding to the antibody in both a dot blot immunoassay and in Biacore binding studies, albeit with lower affinity than the unmodified FLAG sequence.

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  • (PMID = 16889395.001).
  • [ISSN] 8756-7938
  • [Journal-full-title] Biotechnology progress
  • [ISO-abbreviation] Biotechnol. Prog.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antibodies, Monoclonal; 0 / Oligopeptides; 0 / Peptides; 98849-88-8 / FLAG peptide
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81. Yu Y, Alwine JC: Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt. J Virol; 2008 May;82(9):4521-6
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  • We previously showed that wild-type (WT) simian virus 40 (SV40) large T antigen (TAg) inhibits apoptosis via the activation of PI3K/Akt signaling.
  • Here we show that WT TAg expressed from recombinant adenoviruses in U2OS cells induced the phosphorylation of Akt at both T308 and S473.
  • In contrast, Akt phosphorylation was eliminated by the K1 mutation (E107K) within the retinoblastoma protein (Rb) binding motif of TAg.
  • This suggested that Akt phosphorylation may depend on TAg binding to Rb or one of its family members.
  • However, in Rb-negative SAOS2 cells depleted of p107 and p130 by using small hairpin RNAs (shRNAs), WT TAg still mediated Akt phosphorylation.
  • These results suggested that the K1 mutation affects another TAg function.
  • WT-TAg-mediated phosphorylation of Akt was inhibited by a PI3K inhibitor, suggesting that the effects of TAg originated upstream of PI3K; thus, we examined the requirement for insulin receptor substrate 1 (IRS1), which binds and activates PI3K.
  • Depletion of IRS1 by shRNAs abolished the WT-TAg-mediated phosphorylation of Akt.
  • Immunoprecipitation studies showed that the known interaction between TAg and IRS1 is significantly weakened by the K1 mutation.

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  • (PMID = 18305032.001).
  • [ISSN] 1098-5514
  • [Journal-full-title] Journal of virology
  • [ISO-abbreviation] J. Virol.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / R01 CA028379; United States / NIGMS NIH HHS / GM / R01 GM045773; United States / NCI NIH HHS / CA / R01CA28379; United States / NIGMS NIH HHS / GM / R01GM45773
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Adaptor Proteins, Signal Transducing; 0 / Antigens, Viral, Tumor; 0 / IRS1 protein, human; 0 / Insulin Receptor Substrate Proteins; 0 / Retinoblastoma Protein; EC 2.7.11.1 / Proto-Oncogene Proteins c-akt
  • [Other-IDs] NLM/ PMC2293033
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82. Liu Z, Dang Z, Luo J, Yin H, Ahmed JS, Seitzer U: Small-scale expressed sequence tag analysis of Theileria uilenbergi: identification of a gene family encoding potential antigenic proteins. Ann N Y Acad Sci; 2008 Dec;1149:214-7
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  • [Title] Small-scale expressed sequence tag analysis of Theileria uilenbergi: identification of a gene family encoding potential antigenic proteins.
  • [MeSH-major] Antigens, Protozoan / genetics. Expressed Sequence Tags. Genes, Protozoan. Theileria / genetics

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  • (PMID = 19120214.001).
  • [ISSN] 1749-6632
  • [Journal-full-title] Annals of the New York Academy of Sciences
  • [ISO-abbreviation] Ann. N. Y. Acad. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Protozoan; 0 / DNA, Complementary
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83. Masoudi-Nejad A, Goto S, Jauregui R, Ito M, Kawashima S, Moriya Y, Endo TR, Kanehisa M: EGENES: transcriptome-based plant database of genes with metabolic pathway information and expressed sequence tag indices in KEGG. Plant Physiol; 2007 Jun;144(2):857-66
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  • [Title] EGENES: transcriptome-based plant database of genes with metabolic pathway information and expressed sequence tag indices in KEGG.
  • EGENES is a knowledge-based database for efficient analysis of plant expressed sequence tags (ESTs) that was recently added to the KEGG suite of databases.
  • [MeSH-major] Databases, Nucleic Acid. Expressed Sequence Tags. Genes, Plant. Metabolic Networks and Pathways / genetics. Plants / genetics

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  • (PMID = 17468225.001).
  • [ISSN] 0032-0889
  • [Journal-full-title] Plant physiology
  • [ISO-abbreviation] Plant Physiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Other-IDs] NLM/ PMC1914165
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84. Chattopadhaya S, Abu Bakar FB, Yao SQ: Expanding the chemical biologist's tool kit: chemical labelling strategies and its applications. Curr Med Chem; 2009;16(34):4527-43
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  • Although the use of genetically encoded fusions to fluorescent proteins have greatly advanced such studies, the large size of these tags and their ability to perturb protein activity has been major limitations.
  • Thus, chemical labelling approaches seek to "decorate" biomolecules in live cells through the site-specific introduction of a small, non-native chemical tag (or reporter group).
  • The introduced tag is minimally invasive such that the activity and/or function of the target molecule in not perturbed/compromised by its inclusion.
  • In most cases, this modification is brought about by fusing target biomolecules to protein domains/peptide tags or via the incorporation of reactive "handles" by either exploiting the cell's biosynthetic machinery or during protein synthesis.
  • The versatility of these approaches has been demonstrated by their ability to tag not just proteins but also intractable biomolecules like lipids and glycans.
  • In this review, we summarise the various strategies available to "chemically" tag proteins and provide a comparative analysis their advantages and disadvantages.

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  • (PMID = 19903152.001).
  • [ISSN] 1875-533X
  • [Journal-full-title] Current medicinal chemistry
  • [ISO-abbreviation] Curr. Med. Chem.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Fluorescent Dyes; 0 / Proteins; 0 / Recombinant Fusion Proteins
  • [Number-of-references] 114
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85. Jin ML, Li X, Luo J, Zhao HY, Liu Y: [Relationship between the malignant mesothelioma and simian virus 40 in China: a study of 17 cases]. Zhonghua Bing Li Xue Za Zhi; 2006 Oct;35(10):602-5
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  • These samples were also immunohistochemically evaluated for expression of SV40 TAg protein with two different anti-SV40 Tag (Pab101 and Ab-2).
  • No immunopositive staining for SV40 TAg was found in any of the samples.

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  • (PMID = 17134568.001).
  • [ISSN] 0529-5807
  • [Journal-full-title] Zhonghua bing li xue za zhi = Chinese journal of pathology
  • [ISO-abbreviation] Zhonghua Bing Li Xue Za Zhi
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Antigens, Viral, Tumor
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86. Krcmár J, Kotolová H, Veselá L, Csöllei J, Gonec T, Karpísek M, Bartosová L, Necas J: [Study of a newly synthesized substance with a potential to stimulate beta3-adrenergic receptors]. Ceska Slov Farm; 2006 May;55(3):120-3
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  • [Title] [Study of a newly synthesized substance with a potential to stimulate beta3-adrenergic receptors].
  • The results indicated a significant reduction in serum glucose levels (-26 %, p<0,01), triacylglyceride levels (-21 %, p<0,05) and leptin levels (-43 %, p<0,01).
  • Further the effect of a single intraperitoneal dose (1 mg/kg) of B496 and BRL-37344 on serum leptin levels in the C57Bl/6J mouse was investigated.

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  • (PMID = 16838489.001).
  • [ISSN] 1210-7816
  • [Journal-full-title] Ceská a Slovenská farmacie : casopis Ceské farmaceutické spolecnosti a Slovenské farmaceutické spolecnosti
  • [ISO-abbreviation] Ceska Slov Farm
  • [Language] cze
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Czech Republic
  • [Chemical-registry-number] 0 / Adrenergic beta-Agonists; 0 / B496 compound; 0 / Blood Glucose; 0 / Ethanolamines; 0 / Leptin; 0 / Phenoxyacetates; 0 / Receptors, Adrenergic, beta-3; 0 / Triglycerides; 114333-71-0 / BRL 37344; 97C5T2UQ7J / Cholesterol
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87. Yorty JL, Tevethia SS, Schell TD: Rapid accumulation of adoptively transferred CD8+ T cells at the tumor site is associated with long-term control of SV40 T antigen-induced tumors. Cancer Immunol Immunother; 2008 Jun;57(6):883-95
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  • We previously established a model to study CD8(+) T cell (T(CD8))-based adoptive immunotherapy of cancer using line SV11 mice that develop choroid plexus tumors in the brain due to transgenic expression of Simian Virus 40 large T antigen (Tag).
  • These mice are tolerant to the three dominant T(CD8)-recognized Tag epitopes I, II/III and IV.
  • However, adoptive transfer of spleen cells from naïve C57BL/6 (B6) mice prolongs SV11 survival following T(CD8) priming against the endogenous Tag epitope IV.
  • In addition, survival of SV11 mice is dramatically increased following transfer of lymphocytes from Tag-immune B6 mice.
  • In the current study, we compared the kinetics and magnitude of Tag-specific T(CD8) accumulation at the tumor site following adoptive transfer with a high dose of either Tag-immune or naïve donor cells or decreasing doses of Tag-immune lymphocytes.
  • Following adoptive transfer of Tag-immune cells, epitope I- and IV-specific T(CD8) accumulated to high levels in the brain of SV11 mice, peaking at 5-7 days, while epitope IV-specific T(CD8 )derived from naïve donors required three weeks to achieve peak levels.
  • A similar delay in the peak of epitope IV-specific T(CD8) accumulation was observed when tenfold fewer Tag-immune donor cells were administered, reducing control of tumor progression.
  • We also provide evidence that although multiple specificities are represented in the Tag immune donor lymphocytes, epitope IV-specific donor T(CD8) play a predominant role in control of tumor growth.

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  • (PMID = 18004562.001).
  • [ISSN] 0340-7004
  • [Journal-full-title] Cancer immunology, immunotherapy : CII
  • [ISO-abbreviation] Cancer Immunol. Immunother.
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / R37 CA025000; United States / NCI NIH HHS / CA / R01 CA025000-30; United States / NCI NIH HHS / CA / R01 CA025000; United States / NCI NIH HHS / CA / CA25000; None / None / / R01 CA025000-30
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Antigens, Polyomavirus Transforming; 0 / Cytokines; 0 / Epitopes; 0 / Peptides
  • [Other-IDs] NLM/ NIHMS237232; NLM/ PMC2958109
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88. Saito S, Nakagiri H, Watanabe H, Matsuo N, Tokimitsu I, Okazaki M: Size-based distributions of postprandial lipoproteins in lymph and serum after oral administration of triacylglycerol and diacylglycerol oils in rats. J Nutr Sci Vitaminol (Tokyo); 2008 Dec;54(6):491-6
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  • Several studies in humans and rodents suggest that postprandial serum triglyceride (TG) levels are decreased by a single oral administration of diacylglycerol (DAG) oil compared with administration of control triacylglycerol (TAG) oil.
  • In thoracic duct lymph pooled for 3 h after oral administration of TAG or DAG, the size-based distributions of postprandial lymphatic lipoprotein-TG and -cholesterol levels did not differ significantly, suggesting that DAG did not affect the size of lipoprotein particles secreted from the small intestine.
  • Serum lipoprotein-TG (60%) and -cholesterol levels (90%), however, were significantly different among fractions with a diameter of greater than 80 nm 1 to 2 h after the administration of DAG compared to TAG.
  • Thus, compared with TAG, dietary DAG may reduce the postprandial serum total TG levels.

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  • (PMID = 19155588.001).
  • [ISSN] 0301-4800
  • [Journal-full-title] Journal of nutritional science and vitaminology
  • [ISO-abbreviation] J. Nutr. Sci. Vitaminol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Japan
  • [Chemical-registry-number] 0 / Chylomicrons; 0 / Dietary Fats; 0 / Diglycerides; 0 / Lipoproteins; 0 / Triglycerides; 97C5T2UQ7J / Cholesterol
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89. Osako K, Saito H, Hossain MA, Kuwahara K, Okamoto A: Docosahexaenoic acid levels in the lipids of spotted mackerel Scomber australasicus. Lipids; 2006 Jul;41(7):713-20
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  • DHA was characteristically the major FA of all the major lipid classes of all organs except for liver TAG.
  • In particular, the DHA levels in the TAG from all organs of S. australasicus accounted for up to 17% of TFA, even though it is a neutral depot lipid. S. australasicus contained markedly high levels of DHA, even though it is a small-sized Scombridae species, and its high levels of DHA were close to those in large-sized highly migratory tuna species.
  • Furthermore, DHA levels in its muscle TAG were consistently high, compared with those in the visceral TAG, which might be directly influenced by the prey lipids.

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  • (PMID = 17069355.001).
  • [ISSN] 0024-4201
  • [Journal-full-title] Lipids
  • [ISO-abbreviation] Lipids
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Lipids; 25167-62-8 / Docosahexaenoic Acids
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90. Hinnerwisch J, Fenton WA, Furtak KJ, Farr GW, Horwich AL: Loops in the central channel of ClpA chaperone mediate protein binding, unfolding, and translocation. Cell; 2005 Jul 1;121(7):1029-41
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  • The cylindrical Hsp100 chaperone ClpA mediates ATP-dependent unfolding of substrate proteins bearing "tag" sequences, such as the 11-residue ssrA sequence appended to proteins translationally stalled at ribosomes.
  • Whereas a tag from RepA protein crosslinked proximally to the flexible N domains, the ssrA sequence in GFP-ssrA crosslinked distally in the channel to a segment of the distal ATPase domain (D2).
  • Single substitutions placed in this D2 loop, and also in two apparently cooperating proximal (D1) loops, abolished binding of ssrA substrates and unfolded proteins lacking tags and blocked unfolding of GFP-RepA.


91. Han S, Kim J, Park CH, Yoon HC, Heo J: Optimal Detection Range of RFID Tag for RFID-based Positioning System Using the k-NN Algorithm. Sensors (Basel); 2009;9(6):4543-58
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  • [Title] Optimal Detection Range of RFID Tag for RFID-based Positioning System Using the k-NN Algorithm.
  • An RFID-based positioning system using the k-nearest neighbor (k-NN) algorithm can determine the position of a moving reader from observed reference data.
  • In this study, the optimal detection range of an RFID-based positioning system was determined on the principle that tag spacing can be derived from the detection range.
  • It was assumed that reference tags without signal strength information are regularly distributed in 1-, 2- and 3-dimensional spaces.
  • The optimal detection range was determined, through analytical and numerical approaches, to be 125% of the tag-spacing distance in 1-dimensional space.

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  • (PMID = 22408540.001).
  • [ISSN] 1424-8220
  • [Journal-full-title] Sensors (Basel, Switzerland)
  • [ISO-abbreviation] Sensors (Basel)
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Switzerland
  • [Other-IDs] NLM/ PMC3291925
  • [Keywords] NOTNLM ; RFID (major topic) / detection range (major topic) / k-nearest neighbor (major topic) / ubiquitous positioning system (major topic)
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92. Chen GQ, Jiang Y, Chen F: Variation of lipid class composition in Nitzschia laevis as a response to growth temperature change. Food Chem; 2008 Jul 1;109(1):88-94
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  • Higher temperature up to 23°C benefited the growth of N. laevis but only had a slight influence on EPA and lipid contents.
  • Under all temperatures tested, triacylglycerol (TAG) was the predominant lipid constituent (64.5-69.1% of total lipid) and was highly saturated.
  • In sharp contrast to TAG, PC was highly unsaturated and contained a higher amount of EPA under lower temperature.

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  • [Copyright] Copyright © 2007 Elsevier Ltd. All rights reserved.
  • (PMID = 26054268.001).
  • [ISSN] 0308-8146
  • [Journal-full-title] Food chemistry
  • [ISO-abbreviation] Food Chem
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Keywords] NOTNLM ; Eicosapentaenoic acid / Lipid / Nitzschia laevis / Temperature
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93. Dubin MJ, Bowler C, Benvenuto G: Overexpressing tagged proteins in plants using a modified gateway cloning strategy. Cold Spring Harb Protoc; 2010 Mar;2010(3):pdb.prot5401
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • In the first step, the gene of interest is cloned into an entry vector containing standardized cloning sites with the desired N- or C-terminal tag and an optimized polylinker sequence.
  • A Gateway recombination reaction is used to transfer the protein-tag fusion from the entry clone to a Gateway destination vector with the desired promoter and selectable marker for the organism of interest.
  • As experimental requirements evolve, constructs for expressing the protein of interest with the desired tag, promoter, and selectable marker or other features can rapidly and easily be created.

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  • (PMID = 20194470.001).
  • [ISSN] 1559-6095
  • [Journal-full-title] Cold Spring Harbor protocols
  • [ISO-abbreviation] Cold Spring Harb Protoc
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Recombinant Fusion Proteins
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94. Zha Y, Leung KH, Lo KK, Fung WY, Ng PW, Shi MG, Yap MK, Yip SP: TGFB1 as a susceptibility gene for high myopia: a replication study with new findings. Arch Ophthalmol; 2009 Apr;127(4):541-8
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  • Seven tag single-nucleotide polymorphisms (SNPs) and 1 coding SNP were genotyped.
  • The tag SNP rs4803455 in intron 2 was found to account for the positive results of the other 3 SNPs by stepwise logistic regression.

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  • [CommentIn] Arch Ophthalmol. 2010 Aug;128(8):1081-4 [20697017.001]
  • (PMID = 19365037.001).
  • [ISSN] 1538-3601
  • [Journal-full-title] Archives of ophthalmology (Chicago, Ill. : 1960)
  • [ISO-abbreviation] Arch. Ophthalmol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / TGFB1 protein, human; 0 / Transforming Growth Factor beta1
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95. Azuma Y, Takada M, Shin HW, Kioka N, Nakayama K, Ueda K: Retroendocytosis pathway of ABCA1/apoA-I contributes to HDL formation. Genes Cells; 2009 Feb;14(2):191-204
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  • To address this issue, we expressed human ABCA1 constructs with either an extracellular HA tag or an intracellular GFP tag in cells, and used this system to characterize endocytosis and recycling of ABCA1 and apoA-I.

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  • (PMID = 19170766.001).
  • [ISSN] 1365-2443
  • [Journal-full-title] Genes to cells : devoted to molecular & cellular mechanisms
  • [ISO-abbreviation] Genes Cells
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / ABCA1 protein, human; 0 / APOA1 protein, human; 0 / ATP Binding Cassette Transporter 1; 0 / ATP-Binding Cassette Transporters; 0 / Apolipoprotein A-I; 0 / Cholesterol, HDL; 0 / Clathrin
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96. Yeliseev A, Zoubak L, Gawrisch K: Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor. Protein Expr Purif; 2007 May;53(1):153-63
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  • [Title] Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor.
  • The human peripheral cannabinoid receptor (CB2) was expressed as a fusion with the maltose-binding protein (at the N-terminus), thioredoxin A (at the C-terminus) and two small affinity tags (a Strep-tag and a polyhistidine tag).
  • Expression levels of the recombinant receptor in Escherichia coli BL21(DE3) cells were dependent on location and type of tags in the expression construct, and were as high as 1-2mg per liter of bacterial culture.
  • Maltose-binding protein, thioredoxin and a decahistidine tag were removed from the fusion by treatment with Tobacco etch virus (Tev) protease.
  • Purification to over 90% homogeneity of the resulting CB2, containing an N-terminal Strep-tag was achieved by affinity chromatography on a StrepTactin resin.

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  • (PMID = 17223358.001).
  • [ISSN] 1046-5928
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Grant] United States / Intramural NIH HHS / / Z99 AA999999
  • [Publication-type] Journal Article; Research Support, N.I.H., Intramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Affinity Labels; 0 / Carrier Proteins; 0 / Escherichia coli Proteins; 0 / Maltose-Binding Proteins; 0 / Receptor, Cannabinoid, CB2; 0 / Recombinant Fusion Proteins; 0 / TrxA protein, E coli; 4QD397987E / Histidine; 52500-60-4 / Thioredoxins; 9013-20-1 / Streptavidin; EC 3.4.- / Endopeptidases; EC 3.4.- / TEV protease
  • [Other-IDs] NLM/ NIHMS22044; NLM/ PMC1906715
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97. Senf F, Tommassen J, Koster M: Polar secretion of proteins via the Xcp type II secretion system in Pseudomonas aeruginosa. Microbiology; 2008 Oct;154(Pt 10):3025-32
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • The subcellular localization of the major type II secretion system of Pseudomonas aeruginosa, the Xcp system, was studied microscopically using a biarsenical ligand that becomes fluorescent upon binding to a tetracysteine motif (Lumio tag), which was fused to several Xcp components.
  • Fusion of the Lumio tag to the C termini of the XcpR and XcpS proteins did not affect the functionality of these proteins.
  • Fluorescence microscopy showed that they were predominantly localized to the poles of P. aeruginosa cells, when produced at levels comparable to chromosomally encoded XcpR and XcpS.

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  • (PMID = 18832308.001).
  • [ISSN] 1350-0872
  • [Journal-full-title] Microbiology (Reading, England)
  • [ISO-abbreviation] Microbiology (Reading, Engl.)
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / DNA, Bacterial; 0 / Membrane Transport Proteins; 0 / Recombinant Fusion Proteins; 0 / xcpR protein, Pseudomonas aeruginosa; 147336-22-9 / Green Fluorescent Proteins; EC 3.4.- / Endopeptidases; EC 3.4.21.36 / Pancreatic Elastase; EC 3.4.99.- / alkaline protease
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98. Li C, Yi-Hong, Tan YX, Ai JH, Zhou H, Li SJ, Zhang L, Xia QC, Wu JR, Wang HY, Zeng R: Analysis of microdissected cells by two-dimensional LC-MS approaches. Methods Mol Biol; 2008;428:193-208
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  • We combined the LCM with isotope-coded affinity tag (ICAT) technology and two-dimensional liquid chromatography to investigate the qualitative and quantitative proteomes of hepatocellular carcinoma (HCC).
  • The solubilized proteins from microdissected HCC and non-HCC hepatocytes were qualitatively and quantitatively analyzed with two-dimensional liquid chromatography tandem mass spectrometry (2D-LC-MS/MS) alone or coupled with cleavable isotope-coded affinity tag (cICAT) labeling technology.

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  • (PMID = 18287775.001).
  • [ISSN] 1064-3745
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Neoplasm Proteins; 0 / Proteome
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99. Kraut DA, Prakash S, Matouschek A: To degrade or release: ubiquitin-chain remodeling. Trends Cell Biol; 2007 Sep;17(9):419-21
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  • Hul5 promotes degradation by extending the number of ubiquitin moieties in the tag on substrates, whereas Ubp6 antagonizes degradation by trimming ubiquitin from the tag.

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  • Saccharomyces Genome Database. Saccharomyces Genome Database .
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  • (PMID = 17900906.001).
  • [ISSN] 1879-3088
  • [Journal-full-title] Trends in cell biology
  • [ISO-abbreviation] Trends Cell Biol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Saccharomyces cerevisiae Proteins; 120904-94-1 / Polyubiquitin; EC 3.4.- / Endopeptidases; EC 3.4.25.1 / Proteasome Endopeptidase Complex; EC 3.4.99.- / UBP6 protein, S cerevisiae; EC 6.3.2.- / HUL5 protein, S cerevisiae; EC 6.3.2.19 / Ubiquitin-Protein Ligases
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100. Roda B, Lanzoni G, Alviano F, Zattoni A, Costa R, Di Carlo A, Marchionni C, Franchina M, Ricci F, Tazzari PL, Pagliaro P, Scalinci SZ, Bonsi L, Reschiglian P, Bagnara GP: A novel stem cell tag-less sorting method. Stem Cell Rev; 2009 Dec;5(4):420-7
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  • [Title] A novel stem cell tag-less sorting method.
  • Growing interest in stem cell research has led to the development of a number of new methods for isolation.
  • This paper presents a fractionation protocol based on a tag-less, flow-assisted method of purifying, distinguishing and sorting MSCs.
  • Applications such as MSC purification from epithelial contaminants, MSC characterization from various human sources and sorting of MSC subpopulations with high differentiation potential are described.
  • [MeSH-minor] Cell Differentiation. Cells, Cultured. Epithelial Cells / cytology. Humans

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  • (PMID = 20058204.001).
  • [ISSN] 1558-6804
  • [Journal-full-title] Stem cell reviews
  • [ISO-abbreviation] Stem Cell Rev
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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