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6. Burdge GC, Lillycrop KA, Jackson AA, Gluckman PD, Hanson MA: The nature of the growth pattern and of the metabolic response to fasting in the rat are dependent upon the dietary protein and folic acid intakes of their pregnant dams and post-weaning fat consumption. Br J Nutr; 2008 Mar;99(3):540-9
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  • The PR offspring fed the Fat(4) diet had lower plasma TAG than the Control offspring, but this relationship was reversed when offspring were fed Fat(10).
  • Increasing the folic acid content of the Control or PR maternal diets induced opposing effects on plasma TAG, NEFA, beta-hydroxybutyrate and glucose concentrations in offspring fed Fat(4).

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  • (PMID = 17761015.001).
  • [ISSN] 0007-1145
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Grant] United Kingdom / British Heart Foundation / / FS/05/064/19525; United Kingdom / British Heart Foundation / /
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Blood Glucose; 0 / Dietary Fats; 0 / Dietary Proteins; 0 / Lipids; 935E97BOY8 / Folic Acid
  • [Other-IDs] NLM/ PMC2493056; NLM/ UKMS1430
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7. Zhang H, Luo X, Kranzler HR, Lappalainen J, Yang BZ, Krupitsky E, Zvartau E, Gelernter J: Association between two mu-opioid receptor gene (OPRM1) haplotype blocks and drug or alcohol dependence. Hum Mol Genet; 2006 Mar 15;15(6):807-19
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  • Frequency distributions of haplotypes (constructed by five tag SNPs) differed significantly for cases and controls (P<0.001 for both AD and DD).
  • Logistic regression analyses confirmed the association between OPRM1 variants and substance dependence, when sex and age of subjects and alleles, genotypes, haplotypes or diplotypes of five tag SNPs were considered.

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  • (PMID = 16476706.001).
  • [ISSN] 0964-6906
  • [Journal-full-title] Human molecular genetics
  • [ISO-abbreviation] Hum. Mol. Genet.
  • [Language] ENG
  • [Grant] United States / NIAAA NIH HHS / AA / K24 AA013736-01; United States / NIAAA NIH HHS / AA / R01 AA011330-07; United States / NIDA NIH HHS / DA / R01 DA012690-02; United States / NIAAA NIH HHS / AA / K24 AA013736-06; United States / NIDA NIH HHS / DA / R01 DA012690-04; United States / NIDA NIH HHS / DA / R01 DA012849-02; United States / NIAAA NIH HHS / AA / AA013736-06; United States / NIAAA NIH HHS / AA / K08 AA013732-04; None / None / / R01 DA012849-10; United States / NIAAA NIH HHS / AA / AA013736-02; United States / NIDA NIH HHS / DA / R01 DA012690; United States / NIAAA NIH HHS / AA / P50 AA012870-07; United States / NIAAA NIH HHS / AA / AA013736-07; United States / NIDA NIH HHS / DA / K24-DA15105; United States / NIDA NIH HHS / DA / K24 DA015105-01A1; United States / NIDA NIH HHS / DA / K24 DA015105-05; United States / NIDA NIH HHS / DA / R01 DA012690-07; United States / NIAAA NIH HHS / AA / AA012870-10S1; United States / NIAAA NIH HHS / AA / K08 AA013732-03; United States / NIAAA NIH HHS / AA / P50 AA012870-03; United States / NIDA NIH HHS / DA / R01-DA12849; United States / NIDA NIH HHS / DA / K24 DA015105-04; United States / NIAAA NIH HHS / AA / AA012870-06; United States / NIAAA NIH HHS / AA / K24 AA013736-05; United States / NIAAA NIH HHS / AA / P50 AA012870-10S1; United States / NIDA NIH HHS / DA / R01 DA012849; United States / NIAAA NIH HHS / AA / AA013732-04; United States / NIAAA NIH HHS / AA / R01 AA011330-12; United States / NCRR NIH HHS / RR / M01-RR06192; United States / NIAAA NIH HHS / AA / AA013736-05; United States / NIAAA NIH HHS / AA / K24 AA013736-03; United States / NIAAA NIH HHS / AA / K24 AA013736-07; United States / NIAAA NIH HHS / AA / R01 AA011330-06; United States / NIDA NIH HHS / DA / R01 DA012690-03S1; United States / NIDA NIH HHS / DA / K24 DA015105; United States / NIAAA NIH HHS / AA / P50- AA12870; None / None / / R01 AA011330-12; United States / NIAAA NIH HHS / AA / AA013736-08; United States / NIAAA NIH HHS / AA / P50 AA012870-01; United States / NIDA NIH HHS / DA / R01 DA012690-05; United States / NIAAA NIH HHS / AA / P50 AA012870-06; United States / NIAAA NIH HHS / AA / P50 AA012870-09; United States / NIAAA NIH HHS / AA / R01 AA011330-10; United States / NIDA NIH HHS / DA / R01 DA012849-01; United States / NIAAA NIH HHS / AA / AA011330-03; United States / NIAAA NIH HHS / AA / P50 AA012870-02; United States / NIAAA NIH HHS / AA / P50 AA012870-08; United States / NIAAA NIH HHS / AA / K24 AA013736-11; United States / NIAAA NIH HHS / AA / P50 AA012870-10; United States / NIDA NIH HHS / DA / R01 DA012849-05S1; United States / NIAAA NIH HHS / AA / R01-AA11330; United States / NIAAA NIH HHS / AA / AA012870-08; United States / NIAAA NIH HHS / AA / K08 AA013732-02; United States / NIAAA NIH HHS / AA / AA013736-04; United States / NIAAA NIH HHS / AA / K24 AA013736; United States / NIAAA NIH HHS / AA / AA012870-07; United States / NIDA NIH HHS / DA / R01 DA012690-08; United States / NCRR NIH HHS / RR / M01 RR006192; United States / NIDA NIH HHS / DA / R01-DA12690; United States / NIDA NIH HHS / DA / R01 DA012849-10; United States / NIDA NIH HHS / DA / R01 DA012690-02S2; United States / NIDA NIH HHS / DA / R01 DA012849-04; United States / NIAAA NIH HHS / AA / K08 AA013732; United States / NIAAA NIH HHS / AA / R01 AA011330-04A2; United States / NIAAA NIH HHS / AA / AA012870-03; United States / NIDA NIH HHS / DA / R01 DA012690-06; United States / NIDA NIH HHS / DA / K24 DA015105-02; United States / NIAAA NIH HHS / AA / K08-AA13732; United States / NIAAA NIH HHS / AA / P50 AA012870-05; United States / NIAAA NIH HHS / AA / AA012870-04; United States / NIDA NIH HHS / DA / R01 DA012690-10; United States / NIAAA NIH HHS / AA / R01 AA011330-05; United States / NIAAA NIH HHS / AA / P50 AA012870-04; United States / NIDA NIH HHS / DA / R01 DA012690-09; United States / NIDA NIH HHS / DA / K24 DA015105-03; United States / NIAAA NIH HHS / AA / K24 AA013736-08; United States / NIDA NIH HHS / DA / R01 DA012849-06A2; United States / NIAAA NIH HHS / AA / K24-AA13736; United States / NIAAA NIH HHS / AA / R01 AA011330; United States / NIAAA NIH HHS / AA / AA013736-01; United States / NIAAA NIH HHS / AA / AA013736-03; United States / NIAAA NIH HHS / AA / R01 AA011330-03; United States / NIDA NIH HHS / DA / R01 DA012690-01A1; United States / NIAAA NIH HHS / AA / AA012870-02; United States / NIDA NIH HHS / DA / R01 DA012849-05; United States / NIAAA NIH HHS / AA / AA013732-02; United States / NIDA NIH HHS / DA / R01 DA012849-09; United States / NIDA NIH HHS / DA / R01 DA012849-08; United States / NIAAA NIH HHS / AA / K24 AA013736-04; United States / NIAAA NIH HHS / AA / K08 AA013732-01; United States / NIAAA NIH HHS / AA / K24 AA013736-10; United States / NIAAA NIH HHS / AA / P50 AA012870-11; United States / NIAAA NIH HHS / AA / R01 AA011330-11; United States / NIAAA NIH HHS / AA / AA012870-05; United States / NIAAA NIH HHS / AA / R01 AA011330-09A2; United States / NIAAA NIH HHS / AA / P50 AA012870; United States / NIAAA NIH HHS / AA / AA013732-03; United States / NIDA NIH HHS / DA / R01 DA012849-07; United States / NIAAA NIH HHS / AA / K24 AA013736-09; United States / NIDA NIH HHS / DA / R01 DA012849-04S1; United States / NIAAA NIH HHS / AA / AA013732-01; United States / NIDA NIH HHS / DA / R01 DA012849-03; United States / NIDA NIH HHS / DA / R01 DA012690-02S1; United States / NIDA NIH HHS / DA / R01 DA012690-03; United States / NIAAA NIH HHS / AA / AA013736-11; United States / NIAAA NIH HHS / AA / K24 AA013736-02; United States / NIAAA NIH HHS / AA / P50 AA012870-12; None / None / / R01 DA012690-10; United States / NIAAA NIH HHS / AA / R01 AA011330-08
  • [Publication-type] Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] England
  • [Chemical-registry-number] 0 / OPRM1 protein, human; 0 / Receptors, Opioid, mu
  • [Other-IDs] NLM/ NIHMS317657; NLM/ PMC3164878
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8. Herzka DA, Derbyshire JA, Kellman P, McVeigh ER: Single heartbeat cardiac tagging for the evaluation of transient phenomena. Magn Reson Med; 2005 Dec;54(6):1455-64
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  • Many cardiac abnormalities are of a transient nature, creating a beat-to-beat variation in myocardial function.
  • All information necessary for the reconstruction of a cine loop is acquired within a single heartbeat, avoiding the temporal blurring introduced by segmented imaging due to the assumption of cardiac cycle periodicity.
  • Images reconstructed from such data displayed tag blurring and reduced tag persistence due to motion and inter-heartbeat variability.

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  • (PMID = 16265635.001).
  • [ISSN] 0740-3194
  • [Journal-full-title] Magnetic resonance in medicine
  • [ISO-abbreviation] Magn Reson Med
  • [Language] eng
  • [Grant] United States / Intramural NIH HHS / / Z01 HL004608-08
  • [Publication-type] Clinical Trial; Journal Article
  • [Publication-country] United States
  • [Other-IDs] NLM/ NIHMS26912; NLM/ PMC2034344
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9. Nagee A, Acharya A, Shete A, Mukhopadhyaya PN, Aich BA: Molecular characterization of an expressed sequence tag representing the cuticle-degrading serine protease gene (PII) from the nematophagous fungus Arthrobotrys oviformis by differential display technology. Genet Mol Res; 2008;7(4):1200-8
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  • [Title] Molecular characterization of an expressed sequence tag representing the cuticle-degrading serine protease gene (PII) from the nematophagous fungus Arthrobotrys oviformis by differential display technology.
  • The fragment was converted into an expressed sequence tag (EST) through characterization at the molecular level.
  • [MeSH-major] Ascomycota / enzymology. Ascomycota / genetics. Expressed Sequence Tags. Fungal Proteins / genetics. Gene Expression Profiling / methods. Serine Endopeptidases / genetics

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  • (PMID = 19048499.001).
  • [ISSN] 1676-5680
  • [Journal-full-title] Genetics and molecular research : GMR
  • [ISO-abbreviation] Genet. Mol. Res.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Brazil
  • [Chemical-registry-number] 0 / Fungal Proteins; 0 / RNA, Messenger; EC 3.4.21.- / Serine Endopeptidases
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10. Shimp RL Jr, Martin LB, Zhang Y, Henderson BS, Duggan P, MacDonald NJ, Lebowitz J, Saul A, Narum DL: Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP1(42)) in the absence of an affinity tag. Protein Expr Purif; 2006 Nov;50(1):58-67
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  • [Title] Production and characterization of clinical grade Escherichia coli derived Plasmodium falciparum 42 kDa merozoite surface protein 1 (MSP1(42)) in the absence of an affinity tag.
  • To date, all of the reported recombinant proteins include a 6 x His affinity tag to facilitate purification, including three MSP1(42) clinical grade proteins currently in human trials.
  • Under some circumstances, the presence of the 6 x His tag may not be desirable.
  • Therefore, we were interested to produce clinical grade MSP1(42) without a 6 x His affinity tag from E. coli inclusion bodies.
  • We produced a recombinant MSP1(42) with a P. falciparum FUP (Uganda-Palo Alto) phenotype which accounts for a substantial proportion of the MSP1(42) protein observed in African isolates.

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  • (PMID = 16884920.001).
  • [ISSN] 1046-5928
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Grant] United States / Intramural NIH HHS / /
  • [Publication-type] Journal Article; Research Support, N.I.H., Intramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Merozoite Surface Protein 1; 0 / Recombinant Proteins
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11. Jayashree B, Jagadeesh VT, Hoisington D: cisprimertool: software to implement a comparative genomics strategy for the development of conserved intron scanning (CIS) markers. Mol Ecol Resour; 2008 May;8(3):575-7
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  • We report here a software tool (cisprimertool) for the identification of conserved intron scanning regions using expressed sequence tag alignments to a completely sequenced model crop genome.

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  • [Copyright] © 2007 ICRISAT.
  • (PMID = 21585836.001).
  • [ISSN] 1755-098X
  • [Journal-full-title] Molecular ecology resources
  • [ISO-abbreviation] Mol Ecol Resour
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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12. Genovese F, Ferrari S, Guaitoli G, Caselli M, Costi MP, Ponterini G: Dimer-monomer equilibrium of human thymidylate synthase monitored by fluorescence resonance energy transfer. Protein Sci; 2010 May;19(5):1023-30
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  • It is applied to tag the two subunits of human thymidylate synthase at cysteines 43 and 43' with an excitation energy donor/acceptor pair.

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  • (PMID = 20306493.001).
  • [ISSN] 1469-896X
  • [Journal-full-title] Protein science : a publication of the Protein Society
  • [ISO-abbreviation] Protein Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] EC 2.1.1.45 / Thymidylate Synthase
  • [Other-IDs] NLM/ PMC2868244
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13. Zai CC, Tiwari AK, King N, De Luca V, Mueller DJ, Shaikh S, Wong GW, Meltzer HY, Lieberman JA, Kennedy JL: Association study of the gamma-aminobutyric acid type a receptor gamma2 subunit gene with schizophrenia. Schizophr Res; 2009 Oct;114(1-3):33-8
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  • Schizophrenia (SCZ) is a severe neuropsychiatric disorder with a strong genetic basis.
  • We analyzed eight GABRG2 and one DRD5 tag single-nucleotide polymorphisms for association with SCZ in 109 small nuclear families and 229 independent SCZ case-control pairs.

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  • (PMID = 19682861.001).
  • [ISSN] 1573-2509
  • [Journal-full-title] Schizophrenia research
  • [ISO-abbreviation] Schizophr. Res.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / GABRG2 protein, human; 0 / Receptors, GABA-A; EC 2.7.11.1 / LRRK2 protein, human; EC 2.7.11.1 / Protein-Serine-Threonine Kinases
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1
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4. Ordu EB, Karagüler NG: Improving the purification of NAD+-dependent formate dehydrogenase from Candida methylica. Prep Biochem Biotechnol; 2007;37(4):333-41
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  • The Candida methylica (cm) recombinant wild type formate dehydrogenase (FDH) gene has been cloned into the pQE-2 TAGZyme expression vector and the 6xHis-tagged FDH gene has been overexpressed in JM105 cells to purify the FDH protein more efficiently, by the use of exopeptidases, TAGZyme Purification System, which has allowed the complete removal of the small N-terminal His-tag.

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  • (PMID = 17849288.001).
  • [ISSN] 1082-6068
  • [Journal-full-title] Preparative biochemistry & biotechnology
  • [ISO-abbreviation] Prep. Biochem. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Recombinant Proteins; 0U46U6E8UK / NAD; 4QD397987E / Histidine; EC 1.2.1.2 / Formate Dehydrogenases
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15. Viégas FB, Wattenberg M, Feinberg J: Participatory visualization with Wordle. IEEE Trans Vis Comput Graph; 2009 Nov-Dec;15(6):1137-44
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  • Wordle creates tag-cloud-like displays that give careful attention to typography, color, and composition.
  • We then present the results of a study of Wordle usage, based both on spontaneous behaviour observed in the wild, and on a large-scale survey of Wordle users.

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  • (PMID = 19834182.001).
  • [ISSN] 1077-2626
  • [Journal-full-title] IEEE transactions on visualization and computer graphics
  • [ISO-abbreviation] IEEE Trans Vis Comput Graph
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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16. Zhou Y, Wu Y, Song J, Ding Y, Hu X, Zhang Z: Crystallization and preliminary X-ray analysis of fluorescent protein mBanana. Protein Pept Lett; 2008;15(1):113-4
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  • After cleavage of the His tag by TEV protease, the mBanana was further purified and crystallized by the hanging-drop vapor-diffusion technique.

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  • (PMID = 18221021.001).
  • [ISSN] 0929-8665
  • [Journal-full-title] Protein and peptide letters
  • [ISO-abbreviation] Protein Pept. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Luminescent Proteins; 0 / Recombinant Proteins
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17. Campisano A, Schroeder C, Schemionek M, Overhage J, Rehm BH: PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa. Appl Environ Microbiol; 2006 Apr;72(4):3066-8
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  • An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated.
  • A C-terminal, hexahistidine tag fusion enabled the identification of PslD.

  • Gene Ontology. gene/protein/disease-specific - Gene Ontology annotations from this paper .
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  • (PMID = 16598021.001).
  • [ISSN] 0099-2240
  • [Journal-full-title] Applied and environmental microbiology
  • [ISO-abbreviation] Appl. Environ. Microbiol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Bacterial Outer Membrane Proteins
  • [Other-IDs] NLM/ PMC1448989
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18. Hamdane D, Skouloubris S, Myllykallio H, Golinelli-Pimpaneau B: Expression and purification of untagged and histidine-tagged folate-dependent tRNA:m5U54 methyltransferase from Bacillus subtilis. Protein Expr Purif; 2010 Sep;73(1):83-9
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  • The tag did not significantly alter the expression level, flavin content, activity and secondary structure of the protein.

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  • [Copyright] Copyright 2010 Elsevier Inc. All rights reserved.
  • (PMID = 20412857.001).
  • [ISSN] 1096-0279
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / Recombinant Fusion Proteins; 0U46U6E8UK / NAD; 4QD397987E / Histidine; EC 2.1.1.- / tRNA Methyltransferases; EC 2.1.1.35 / tRNA(uracil-5)-methyltransferase
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19. Szakonyi G, Leng D, Ma P, Bettaney KE, Saidijam M, Ward A, Zibaei S, Gardiner AT, Cogdell RJ, Butaye P, Kolsto AB, O'reilly J, Hope RJ, Rutherford NG, Hoyle CJ, Henderson PJ: A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily. J Antimicrob Chemother; 2007 Jun;59(6):1265-70
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Depending upon their topology, proteins are produced with RGS(His)(6)-tag or a Strep-tag at the C terminus.
  • These tags facilitate the purification of the overexpressed proteins in milligram quantities for structural studies.

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  • (PMID = 17412722.001).
  • [ISSN] 0305-7453
  • [Journal-full-title] The Journal of antimicrobial chemotherapy
  • [ISO-abbreviation] J. Antimicrob. Chemother.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Anti-Bacterial Agents; 0 / Bicyclo Compounds, Heterocyclic; 0 / P-Glycoproteins; 4QD397987E / Histidine; J03U9E2P82 / bicozamycin
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20. Van der Auwera G, Baute J, Bauwens M, Peck I, Piette D, Pycke M, Asselman P, Depicker A: Development and application of novel constructs to score C:G-to-T:A transitions and homologous recombination in Arabidopsis. Plant Physiol; 2008 Jan;146(1):22-31
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Small peptide tags (hemagglutinin tag and Strep tag II) and green fluorescent protein were translationally fused to GUS, which offers possibilities to check for mutant GUS production levels.

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  • (PMID = 17921342.001).
  • [ISSN] 0032-0889
  • [Journal-full-title] Plant physiology
  • [ISO-abbreviation] Plant Physiol.
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ AM180563/ AM180564/ AM180565/ AM180566/ AM180567/ AM180568
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 5Z93L87A1R / Guanine; 8J337D1HZY / Cytosine; EC 3.2.1.31 / Glucuronidase; JAC85A2161 / Adenine; VC2W18DGKR / Thymidine
  • [Other-IDs] NLM/ PMC2230553
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21. Nussio MR, Voelcker NH, Miners JO, Lewis BC, Sykes MJ, Shapter JG: AFM study of the interaction of cytochrome P450 2C9 with phospholipid bilayers. Chem Phys Lipids; 2010 Feb;163(2):182-9
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  • Despite lacking the N-terminus transmembrane spanning domain, the CYP2C9 protein appeared to partially embed into the membrane bilayer, as evidenced by an increase in melting temperature of surrounding phospholipids.
  • Reversible binding of CYP2C9 via an engineered His tag to a phospholipid bilayer was facilitated using nickel-chelating lipids, presenting potential applications for biosensor technologies.

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  • (PMID = 19925783.001).
  • [ISSN] 1873-2941
  • [Journal-full-title] Chemistry and physics of lipids
  • [ISO-abbreviation] Chem. Phys. Lipids
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Ireland
  • [Chemical-registry-number] 0 / Lipid Bilayers; 0 / Recombinant Proteins; 7OV03QG267 / Nickel; EC 1.14.13.- / CYP2C9 protein, human; EC 1.14.13.- / Cytochrome P-450 CYP2C9; EC 1.14.14.1 / Aryl Hydrocarbon Hydroxylases; U86ZGC74V5 / Dimyristoylphosphatidylcholine
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22. Yamada S, Miyazaki M, Kanazawa H, Higashi M, Morohoshi Y, Bluml S, McComb JG: Visualization of cerebrospinal fluid movement with spin labeling at MR imaging: preliminary results in normal and pathophysiologic conditions. Radiology; 2008 Nov;249(2):644-52
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  • A time-spatial labeling inversion pulse (SLIP) technique was applied to label, or tag, CSF in a region of interest.

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  • [Copyright] (c) RSNA, 2008.
  • (PMID = 18936318.001).
  • [ISSN] 1527-1315
  • [Journal-full-title] Radiology
  • [ISO-abbreviation] Radiology
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Spin Labels
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23. Saidijam M, Bettaney KE, Szakonyi G, Psakis G, Shibayama K, Suzuki S, Clough JL, Blessie V, Abu-Bakr A, Baumberg S, Meuller J, Hoyle CK, Palmer SL, Butaye P, Walravens K, Patching SG, O'reilly J, Rutherford NG, Bill RM, Roper DI, Phillips-Jones MK, Henderson PJ: Active membrane transport and receptor proteins from bacteria. Biochem Soc Trans; 2005 Aug;33(Pt 4):867-72
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  • Depending upon their topology, proteins are produced with RGSH6 or a Strep tag at the C-terminus.

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  • (PMID = 16042616.001).
  • [ISSN] 0300-5127
  • [Journal-full-title] Biochemical Society transactions
  • [ISO-abbreviation] Biochem. Soc. Trans.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / DNA, Bacterial; 0 / Membrane Transport Proteins; 0 / Symporters
  • [Number-of-references] 26
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24. Backer MV, Levashova Z, Levenson R, Blankenberg FG, Backer JM: Cysteine-containing fusion tag for site-specific conjugation of therapeutic and imaging agents to targeting proteins. Methods Mol Biol; 2008;494:275-94
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  • [Title] Cysteine-containing fusion tag for site-specific conjugation of therapeutic and imaging agents to targeting proteins.
  • Targeted delivery of therapeutic and imaging agents requires conjugation of a corresponding payload to a targeting peptide or protein.
  • We describe here our experience with site-specific protein modification via a novel cysteine-containing fusion tag (Cys-tag), which is a 15-amino-acid (aa) N-terminal fragment of human ribonuclease I with the R4C substitution.
  • Several Cys-tagged proteins and peptides with different numbers of native cysteines were expressed and refolded into functionally active conformation, indicating that the tag does not interfere with the formation of internal disulfide bonds.
  • We expect that Cys-tag would provide new opportunities for development of targeted therapeutic and imaging agents for research and clinical use.

  • Hazardous Substances Data Bank. CYSTEINE .
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  • (PMID = 18726580.001).
  • [ISSN] 1064-3745
  • [Journal-full-title] Methods in molecular biology (Clifton, N.J.)
  • [ISO-abbreviation] Methods Mol. Biol.
  • [Language] eng
  • [Grant] United States / NIBIB NIH HHS / EB / R01 EB000898; United States / NIBIB NIH HHS / EB / R21 EB001946; United States / NCI NIH HHS / CA / R43 CA113080
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Indicators and Reagents; 0 / Liposomes; 0 / Recombinant Fusion Proteins; 0 / Vascular Endothelial Growth Factor A; EC 3.1.27.5 / Ribonuclease, Pancreatic; K848JZ4886 / Cysteine
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25. Traulsen A, Nowak MA: Chromodynamics of cooperation in finite populations. PLoS One; 2007 Mar 07;2(3):e270
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  • BACKGROUND: The basic idea of tag-based models for cooperation is that individuals recognize each other via arbitrary signals, so-called tags.
  • If there are tags of different colors, then cooperators can always establish new signals of recognition.
  • Cooperators use a secret tag until they are discovered by defectors who then destroy cooperation based on this tag.
  • Subsequently, a fraction of the population manages to establish cooperation based on a new tag.
  • METHODOLOGY/PRINCIPAL FINDINGS: We derive a mathematical description of stochastic evolutionary dynamics of tag-based cooperation in populations of finite size.
  • We find that cooperators are more abundant than defectors if b/c > 1+2u/v, where u is the mutation rate changing only the strategy and v is the mutation rate changing strategy and tag.
  • CONCLUSIONS/SIGNIFICANCE: In a genetic model, tag-based cooperation only evolves if a gene encodes both strategy and tag.
  • In a cultural model with equal mutation rates between all possible phenotypes (tags and behaviors), the crucial condition is b/c > (K+1)/(K-1), where K is the number of tags.
  • A larger number of tags requires a smaller benefit-to-cost ratio.
  • In the limit of many different tags, the condition for cooperators to have a higher average abundance than defectors becomes b > c.

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  • (PMID = 17342204.001).
  • [ISSN] 1932-6203
  • [Journal-full-title] PloS one
  • [ISO-abbreviation] PLoS ONE
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / GM078986-01; United States / NIGMS NIH HHS / GM / R01 GM078986; United States / NIGMS NIH HHS / GM / 1R01GM078986-01; United States / NIGMS NIH HHS / GM / R01 GM078986-01
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Other-IDs] NLM/ PMC1803018
  • [General-notes] NLM/ Original DateCompleted: 20070725
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26. Vultur A, Arulanandam R, Turkson J, Niu G, Jove R, Raptis L: Stat3 is required for full neoplastic transformation by the Simian Virus 40 large tumor antigen. Mol Biol Cell; 2005 Aug;16(8):3832-46
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  • To investigate the role of Stat3 (signal transducer and activator of transcription-3) in neoplastic transformation by the Large Tumor antigen of Simian Virus 40 (TAg), murine fibroblasts were rendered deficient in Stat3 activity through expression of a Stat3-specific siRNA or a Cre-loxP recombination system.
  • The results demonstrate that growth rate, formation of foci overgrowing a monolayer of normal cells and colony formation in soft agar were dramatically reduced in Stat3-deficient cells.
  • In addition, TAg expression led to increased Stat3 tyrosine phosphorylation, DNA binding, and transcriptional activity, suggesting that Stat3 is required for TAg-mediated neoplasia.
  • Stat3 activation was prevented by blocking the binding of TAg to pRb (retinoblastoma-susceptibility gene product), whereas genetic ablation of pRb increased Stat3 activity, suggesting that pRb inactivation by TAg might be responsible for the observed Stat3 activation.
  • Stat3 activation by TAg was suppressed after inhibition of c-Src, JAKs or the insulin-like growth factor receptor.
  • Inhibition of Src activity led to Stat3 down-regulation as well as apoptosis of sparsely growing, TAg-transformed cells.
  • Taken together, our results suggest that Stat3 is an important component of a pathway emanating from TAg and leading to neoplastic conversion.

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  • (PMID = 15917293.001).
  • [ISSN] 1059-1524
  • [Journal-full-title] Molecular biology of the cell
  • [ISO-abbreviation] Mol. Biol. Cell
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Viral, Tumor; 0 / RNA, Small Interfering; 0 / Retinoblastoma Protein; 21820-51-9 / Phosphotyrosine; EC 2.7.10.1 / Protein-Tyrosine Kinases
  • [Other-IDs] NLM/ PMC1182320
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27. Hsiao YY, Lin CH, Liu JK, Wong TY, Kuo J: Analysis of Codon Usage Patterns in Toxic Dinoflagellate Alexandrium tamarense through Expressed Sequence Tag Data. Comp Funct Genomics; 2010;2010:138538
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  • [Title] Analysis of Codon Usage Patterns in Toxic Dinoflagellate Alexandrium tamarense through Expressed Sequence Tag Data.
  • We have analyzed synonymous codon usage in the genome of A. tamarense CCMP 1598 for protein-coding sequences from 10865 expressed sequence tags (ESTs).
  • Correspondence analysis of A. tamarense genes based on codon usage showed that the GC content at the third base of synonymous codons (GC3s) was strongly correlated with the first axis (r = 0.93 with P < .001).

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  • (PMID = 21052492.001).
  • [ISSN] 1532-6268
  • [Journal-full-title] Comparative and functional genomics
  • [ISO-abbreviation] Comp. Funct. Genomics
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Egypt
  • [Other-IDs] NLM/ PMC2967832
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28. Zhao T, Harada H, Teramura Y, Tanaka S, Itasaka S, Morinibu A, Shinomiya K, Zhu Y, Hanaoka H, Iwata H, Saji H, Hiraoka M: A novel strategy to tag matrix metalloproteinases-positive cells for in vivo imaging of invasive and metastatic activity of tumor cells. J Control Release; 2010 May 21;144(1):109-14
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  • [Title] A novel strategy to tag matrix metalloproteinases-positive cells for in vivo imaging of invasive and metastatic activity of tumor cells.
  • We constructed a protein-based probe composed of a glutathione-S-transferase (GST)-tag (Inhibitory [I]-domain), a polypeptide as a specific substrate for both MMP2 and MT1-MMP (Cleaved [C]-domain), a transmembrane domain of the epidermal growth factor receptor (Transmembrane [TM]-domain), and DsRed2 (Fluorescent [F]-domain).
  • The present feasibility study opens the door to the development of a novel imaging probe for tumor malignancy using positron emission tomography as well as an optical imaging device.
  • [MeSH-minor] Animals. Cell Line, Tumor. Cell Membrane / metabolism. Cells / metabolism. Cellular Structures / metabolism. Diagnostic Imaging. Endopeptidases / metabolism. Extracellular Matrix / metabolism. HeLa Cells. Humans. Matrix Metalloproteinase 1 / metabolism. Matrix Metalloproteinase 14 / metabolism. Matrix Metalloproteinase 2 / metabolism. Membranes / metabolism. Mice. Mice, Nude. Molecular Imaging. Peptides. Receptor, Epidermal Growth Factor / metabolism. Skin Neoplasms

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  • [Copyright] Copyright 2010 Elsevier B.V. All rights reserved.
  • (PMID = 20096316.001).
  • [ISSN] 1873-4995
  • [Journal-full-title] Journal of controlled release : official journal of the Controlled Release Society
  • [ISO-abbreviation] J Control Release
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Peptides; EC 2.7.10.1 / Receptor, Epidermal Growth Factor; EC 3.4.- / Endopeptidases; EC 3.4.24.- / Matrix Metalloproteinases; EC 3.4.24.24 / Matrix Metalloproteinase 2; EC 3.4.24.7 / Matrix Metalloproteinase 1; EC 3.4.24.80 / Matrix Metalloproteinase 14
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29. Moulder R, Filén JJ, Salmi J, Katajamaa M, Nevalainen OS, Oresic M, Aittokallio T, Lahesmaa R, Nyman TA: A comparative evaluation of software for the analysis of liquid chromatography-tandem mass spectrometry data from isotope coded affinity tag experiments. Proteomics; 2005 Jul;5(11):2748-60
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  • [Title] A comparative evaluation of software for the analysis of liquid chromatography-tandem mass spectrometry data from isotope coded affinity tag experiments.
  • The options available for processing quantitative data from isotope coded affinity tag (ICAT) experiments have mostly been confined to software specific to the instrument of acquisition.
  • The comparisons were made with data from the analysis of a specifically prepared test mixture, nine proteins at a range of relative concentration ratios from 0.1 to 10 (light to heavy labelled forms), as a known control, and data selected from an ICAT study involving the measurement of cytokine induced protein expression in human lymphoblasts, as an applied example.

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  • (PMID = 15952233.001).
  • [ISSN] 1615-9853
  • [Journal-full-title] Proteomics
  • [ISO-abbreviation] Proteomics
  • [Language] eng
  • [Publication-type] Comparative Study; Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Amino Acids; 0 / Peptides; 0 / Proteins
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30. Mysiakina IS, Funtikova NS: [Metabolic characteristics and lipid composition of yeastlike cells and mycelium of Mucor circinelloides var. lusitanicus INMI grown at a high glucose content in the medium]. Mikrobiologiia; 2008 Jul-Aug;77(4):460-4
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  • Yeastlike cells contained more polar lipids and free fatty acids and less principal reserve lipids (triacylglycerides) than mycelial cells; the content of gamma-linolenic acid and the degree of lipid unsaturation were significantly lower in these cells than in the mycelium.

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  • (PMID = 18825971.001).
  • [ISSN] 0026-3656
  • [Journal-full-title] Mikrobiologiia
  • [ISO-abbreviation] Mikrobiologiia
  • [Language] rus
  • [Publication-type] English Abstract; Journal Article
  • [Publication-country] Russia (Federation)
  • [Chemical-registry-number] 0 / Culture Media; 78YC2MAX4O / gamma-Linolenic Acid; EC 1.1.1.41 / Isocitrate Dehydrogenase; EC 4.1.3.1 / Isocitrate Lyase; IY9XDZ35W2 / Glucose
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31. Qin Q, McCallum EJ, Kaas Q, Suda J, Saska I, Craik DJ, Mylne JS: Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis. BMC Genomics; 2010;11:111
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  • [Title] Identification of candidates for cyclotide biosynthesis and cyclisation by expressed sequence tag analysis of Oldenlandia affinis.
  • To increase the speed at which genes involved in cyclotide biosynthesis and processing are being discovered, an expressed sequence tag (EST) project was initiated to survey the transcript profile of O. affinis and to propose some future directions of research on in vivo protein cyclisation.
  • RESULTS: Using flow cytometry the holoploid genome size (1C-value) of O. affinis was estimated to be 4,210 - 4,284 Mbp, one of the largest genomes of the Rubiaceae family.
  • [MeSH-major] Cyclotides / biosynthesis. Expressed Sequence Tags. Oldenlandia / genetics

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  • (PMID = 20158917.001).
  • [ISSN] 1471-2164
  • [Journal-full-title] BMC genomics
  • [ISO-abbreviation] BMC Genomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Cyclotides
  • [Other-IDs] NLM/ PMC2838841
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32. Salek M, Di Bartolo V, Cittaro D, Borsotti D, Wei J, Acuto O, Rappsilber J, Lehmann WD: Sequence tag scanning: a new explorative strategy for recognition of unexpected protein alterations by nanoelectrospray ionization-tandem mass spectrometry. Proteomics; 2005 Feb;5(3):667-74
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  • [Title] Sequence tag scanning: a new explorative strategy for recognition of unexpected protein alterations by nanoelectrospray ionization-tandem mass spectrometry.
  • We consider high abundance fragments formed by cleavage at the C-terminal side of aspartic acid, at the N-terminal side of proline and low mass ions such as a2, b2, b3, y1, y2, and y3.
  • The MS/MS dataset is filtered for each sequence tag of interest by an in silico precursor ion scan.
  • Since the strategy is based on common peptide segments which are shared by different isoforms of peptides it can be applied to the analysis of any post-translational modification or sequence variants of a protein.


33. Hayes CS, Keiler KC: Beyond ribosome rescue: tmRNA and co-translational processes. FEBS Lett; 2010 Jan 21;584(2):413-9
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  • tmRNA is a unique bi-functional RNA that acts as both a tRNA and an mRNA to enter stalled ribosomes and direct the addition of a peptide tag to the C terminus of nascent polypeptides.

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  • (PMID = 19914241.001).
  • [ISSN] 1873-3468
  • [Journal-full-title] FEBS letters
  • [ISO-abbreviation] FEBS Lett.
  • [Language] ENG
  • [Grant] United States / NIGMS NIH HHS / GM / R01 GM068720; United States / NIGMS NIH HHS / GM / GM068720; United States / NIGMS NIH HHS / GM / R01 GM078634-04; United States / NIGMS NIH HHS / GM / R01 GM078634; United States / NIGMS NIH HHS / GM / GM078634-04; United States / NIGMS NIH HHS / GM / GM068720-05; United States / NIGMS NIH HHS / GM / GM078634; United States / NIGMS NIH HHS / GM / R01 GM068720-05
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / RNA, Bacterial; 0 / tmRNA
  • [Number-of-references] 65
  • [Other-IDs] NLM/ NIHMS159891; NLM/ PMC2794900
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34. Maldjian JA, Baer AH, Kraft RA, Laurienti PJ, Burdette JH: Fully automated processing of fMRI data in SPM: from MRI scanner to PACS. Neuroinformatics; 2009;7(1):57-72
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  • It has been used for automated processing of fMRI experiments, as well as for the clinical implementation of fMRI and spin-tag perfusion imaging.

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  • (PMID = 19156543.001).
  • [ISSN] 1559-0089
  • [Journal-full-title] Neuroinformatics
  • [ISO-abbreviation] Neuroinformatics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] S88TT14065 / Oxygen
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35. Harel E, Hilty C, Koen K, McDonnell EE, Pines A: Time-of-flight flow imaging of two-component flow inside a microfluidic chip. Phys Rev Lett; 2007 Jan 5;98(1):017601
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  • Here we report on using NMR imaging and spectroscopy in conjunction with time-of-flight tracking to noninvasively tag and monitor nuclear spins as they flow through the channels of a microfluidic chip.

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  • (PMID = 17358506.001).
  • [ISSN] 0031-9007
  • [Journal-full-title] Physical review letters
  • [ISO-abbreviation] Phys. Rev. Lett.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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36. Duncan CG, Leary RJ, Lin JC, Cummins J, Di C, Schaefer CF, Wang TL, Riggins GJ, Edwards J, Bigner D, Kopelovich L, Vogelstein B, Kinzler KW, Velculescu VE, Yan H: Identification of microbial DNA in human cancer. BMC Med Genomics; 2009 May 08;2:22
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  • This method involves the generation of an experimental DNA tag library through Digital Karyotyping (DK) followed by analysis of the tag sequences for the presence of microbial DNA content using a compiled microbial DNA virtual tag library.
  • We detected DNA from Human herpesvirus 6 (HHV-6) in a DK library of a colorectal cancer liver metastasis and in normal tissue from the same patient.

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  • (PMID = 19426505.001).
  • [ISSN] 1755-8794
  • [Journal-full-title] BMC medical genomics
  • [ISO-abbreviation] BMC Med Genomics
  • [Language] ENG
  • [Grant] United States / NCI NIH HHS / CA / P50 CA108786; United States / NINDS NIH HHS / NS / P50 NS020023; United States / NCI NIH HHS / CA / R01 CA121113; United States / NINDS NIH HHS / NS / R01 NS052507; United States / NCI NIH HHS / CA / P20 CA096890; United States / NCI NIH HHS / CA / P30 CA014236; United States / NCI NIH HHS / CA / R01 CA118822; United States / NCI NIH HHS / CA / R37 CA011898
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Other-IDs] NLM/ PMC2685141
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37. Reyes-Carmona JF, Felippe MS, Felippe WT: Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid. J Endod; 2009 May;35(5):731-6
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  • The apatite formed by the cement-PBS system was deposited within collagen fibrils, promoting controlled mineral nucleation on dentin, observed as the formation of an interfacial layer with tag-like structures.
  • This spontaneous precipitation promotes a biomineralization process that leads to the formation of an interfacial layer with tag-like structures at the cement-dentin interface.

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  • (PMID = 19410094.001).
  • [ISSN] 1878-3554
  • [Journal-full-title] Journal of endodontics
  • [ISO-abbreviation] J Endod
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Aluminum Compounds; 0 / Apatites; 0 / Biocompatible Materials; 0 / Buffers; 0 / Calcium Compounds; 0 / Calcium Phosphates; 0 / Dental Cements; 0 / Drug Combinations; 0 / Oxides; 0 / Phosphates; 0 / Root Canal Filling Materials; 0 / Silicates; 0 / mineral trioxide aggregate; 1304-76-3 / bismuth oxide; 55326-60-8 / carboapatite; 9007-34-5 / Collagen; M4I0D6VV5M / Calcium Chloride; SY7Q814VUP / Calcium; U015TT5I8H / Bismuth
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38. Meng X, Chao J, Xiang Y: Identification from diverse mammalian poxviruses of host-range regulatory genes functioning equivalently to vaccinia virus C7L. Virology; 2008 Mar 15;372(2):372-83
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  • Three C7L homologues (myxoma virus M63R, M64R and cowpox virus 020), when expressed with an epitope tag and from a VACV mutant lacking the host-range genes K1L and C7L (vK1L-C7L-), failed to support productive viral replication in human and murine cells.
  • In contrast, two other C7L homologues, myxoma virus (MYXV) M62R and yaba-like disease virus (YLDV) 67R, when expressed with an epitope tag and from vK1L(-)C7L(-), supported normal viral replication in human and murine cells and restored the ability of the virus to suppress PKR activation.

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  • (PMID = 18054061.001).
  • [ISSN] 0042-6822
  • [Journal-full-title] Virology
  • [ISO-abbreviation] Virology
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / AI065731-01A2; United States / NIAID NIH HHS / AI / R21 AI065731; United States / NIAID NIH HHS / AI / U54 AI057156; United States / NIAID NIH HHS / AI / R21 AI065731-01A2
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / C7L protein, Vaccinia virus; 0 / Viral Nonstructural Proteins; 0 / Viral Proteins
  • [Other-IDs] NLM/ NIHMS43096; NLM/ PMC2276162
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39. Mukhopadhyay T, Ghosh S: Lipid profile and fatty acid composition of two silurid fish eggs. J Oleo Sci; 2007;56(8):399-403
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  • The lipid content of O. pabda and W. attu (on dry basis) are about 14.7% and 17.8% respectively.
  • The major lipid classes are phospolipid (PL) and triacylglycerol (TAG).
  • The O. pabda egg lipid contains more PL while the W. attu egg lipid contains more TAG.
  • Cholesterol content in egg of O. pabda is also higher (about 9.6%) than W. attu (4.1%).


40. Verma R, Cutler DJ, Holmans P, Knowles JA, Crowe RR, Scheftner WA, Weissman MM, DePaulo JR Jr, Levinson DF, Potash JB: Investigating the role of p11 (S100A10) sequence variation in susceptibility to major depression. Am J Med Genet B Neuropsychiatr Genet; 2007 Dec 5;144B(8):1079-82
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  • Recent evidence suggests a potential role for the p11 gene in conferring risk to depressive disorders. p11 has been shown to influence serotonergic transmission, and its expression was found to be reduced in a mouse model of depression, as well as in post-mortem brain tissue from major depressive disorder (MDD) cases.
  • We also assessed common variation by genotyping eight single nucleotide polymorphisms (SNPs), seven tag SNPs and one found through resequencing, in 641 cases and 650 controls.
  • None of the tag SNPs showed any evidence of association.
  • [MeSH-major] Annexin A2 / genetics. Depressive Disorder, Major / genetics. Polymorphism, Single Nucleotide / genetics. Promoter Regions, Genetic / genetics. S100 Proteins / genetics

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  • [Copyright] 2007 Wiley-Liss, Inc.
  • (PMID = 17510952.001).
  • [ISSN] 1552-485X
  • [Journal-full-title] American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics
  • [ISO-abbreviation] Am. J. Med. Genet. B Neuropsychiatr. Genet.
  • [Language] eng
  • [Grant] United States / NIMH NIH HHS / MH / 5R01MH059541; United States / NIMH NIH HHS / MH / 5R01MH059542; United States / NIMH NIH HHS / MH / 5R01MH059552; United States / NIMH NIH HHS / MH / 5R01MH060912; United States / NIMH NIH HHS / MH / 5R01MH061686
  • [Publication-type] Comparative Study; Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Annexin A2; 0 / S100 Proteins; 0 / S100 calcium binding protein A10
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41. Kristensen JB, Jørgensen H, Mu H: Diacylglycerol oil does not affect portal vein transport of nonesterified fatty acids but decreases the postprandial plasma lipid response in catheterized pigs. J Nutr; 2006 Jul;136(7):1800-5
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  • We examined the portal vein transport of NEFA and other lipid related variables, in response to DAG and triacylglycerol (TAG) bolus feeding and a bolus of standard pig feed in 4 portal vein and mesenteric artery catheterized pigs.
  • The postprandial arterial TAG response at 5 and 6 h postprandially was significantly lower after the DAG bolus intake.

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  • (PMID = 16772440.001).
  • [ISSN] 0022-3166
  • [Journal-full-title] The Journal of nutrition
  • [ISO-abbreviation] J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / 1,2-diacylglycerol; 0 / Dietary Fats, Unsaturated; 0 / Diglycerides; 0 / Fatty Acids, Nonesterified; 0 / Triglycerides
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42. Athenstaedt K, Jolivet P, Boulard C, Zivy M, Negroni L, Nicaud JM, Chardot T: Lipid particle composition of the yeast Yarrowia lipolytica depends on the carbon source. Proteomics; 2006 Mar;6(5):1450-9
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  • LP from glucose-grown cells revealed a mean diameter of 650 nm with a hydrophobic core mainly formed of triacylglycerols (TAG) and a minor amount of steryl esters (SE).
  • When cells were grown on oleic acid, LP size increased 3.8-fold, the particles exhibited a significantly lower ratio of TAG to SE, and the relative amount of oleic acid in LP lipids increased compared to cells grown on glucose.

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  • (PMID = 16470660.001).
  • [ISSN] 1615-9853
  • [Journal-full-title] Proteomics
  • [ISO-abbreviation] Proteomics
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Fungal Proteins; 0 / Lipids; 0 / Peptides; 2UMI9U37CP / Oleic Acid; 7440-44-0 / Carbon; IY9XDZ35W2 / Glucose
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43. Asano R, Ikoma K, Kawaguchi H, Ishiyama Y, Nakanishi T, Umetsu M, Hayashi H, Katayose Y, Unno M, Kudo T, Kumagai I: Application of the Fc fusion format to generate tag-free bi-specific diabodies. FEBS J; 2010 Jan;277(2):477-87
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  • [Title] Application of the Fc fusion format to generate tag-free bi-specific diabodies.
  • We previously reported the use of a humanized bi-specific diabody that targets epidermal growth factor receptor and CD3 (hEx3-Db) for cancer immunotherapy.
  • Bacterial expression can be used to express small recombinant antibodies on a large scale; however, their overexpression often results in the formation of insoluble aggregates, and in most cases artificial affinity peptide tags need to be fused to the antibodies for purification by affinity chromatography.
  • Here, we propose a novel method for preparing refined, functional, tag-free bi-specific diabodies from IgG-like bi-specific antibodies (BsAbs) in a mammalian expression system.
  • The BsAb was purified by protein A affinity chromatography, and the refined tag-free hEx3-Db was efficiently produced from the Fc fusion format by protease digestion.
  • The tag-free hEx3-Db from the Fc fusion format showed a greater inhibition of cancer growth than affinity-tagged hEx3-Db prepared directly from Chinese hamster ovary cells.
  • This approach may be used for industrial-scale production of functional tag-free small therapeutic antibodies.

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  • (PMID = 20015073.001).
  • [ISSN] 1742-4658
  • [Journal-full-title] The FEBS journal
  • [ISO-abbreviation] FEBS J.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antibodies, Bispecific; 0 / Immunoglobulin Fc Fragments; 0 / Recombinant Fusion Proteins; EC 3.4.- / Peptide Hydrolases
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44. Chechi K, Yasui N, Ikeda K, Yamori Y, K Cheema S: Flax oil-mediated activation of PPAR-γ correlates with reduction of hepatic lipid accumulation in obese spontaneously hypertensive/NDmcr-cp rats, a model of the metabolic syndrome. Br J Nutr; 2010 Nov;104(9):1313-21
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  • Obese rats exhibited higher body weight, liver weight and mesenteric fat-, epididymal fat- and renal fat-pad weights, and also TAG and cholesterol concentrations in serum and VLDL, LDL and HDL fractions, when compared with the lean rats (P < 0·001), irrespective of the diets.
  • Flax oil feeding also revealed a significant reduction in hepatic TAG and cholesterol concentrations in obese rats compared with the LO rats (P < 0·05).

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  • (PMID = 20546645.001).
  • [ISSN] 1475-2662
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Dietary Fats; 0 / Hypolipidemic Agents; 0 / Insulin; 0 / PPAR gamma; 0 / Plant Oils; 0 / RNA, Messenger; 0 / Thiobarbituric Acid Reactive Substances; 61789-99-9 / lard
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45. Tschantz WR, Pfeifer ND, Meade CL, Wang L, Lanzetti A, Kamath AV, Berlioz-Seux F, Hashim MF: Expression, purification and characterization of the human membrane transporter protein OATP2B1 from Sf9 insect cells. Protein Expr Purif; 2008 Feb;57(2):163-71
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  • Protein expressed by the Sf9-baculovirus expression system is functionally active as demonstrated by saturable uptake kinetics with a K(m) of 5.9+/-0.76 microM for estrone-3-sulfate.
  • OATP2B1 was extracted from Sf9-membranes with ABS-14-4 detergent and purified using a one-step FLAG-tag purification method.
  • C-terminal FLAG-tag purification and immunoblot detection, together with N-terminal sequencing, confirmed the presence of only full-length protein.
  • Physical, biochemical and functional characterization show that purified human OATP2B1 is pure, homogeneous and appropriate for use as a standard to quantitate expression of OATP2B1 in in vitro systems and tissue samples.

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  • (PMID = 18024077.001).
  • [ISSN] 1046-5928
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Detergents; 0 / Organic Anion Transporters; 0 / Recombinant Proteins; 0 / SLCO2B1 protein, human; 2DI9HA706A / Estrone; EC 3.2.1.- / Glycoside Hydrolases; QTL48N278K / estrone sulfate
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46. Yanai H, Tomono Y, Yoshida H, Tada N: [Therapeutic application of diacylglycerol oil for the metabolic syndrome]. Rinsho Byori; 2010 Jan;58(1):39-44
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  • Diacylglycerol(DAG) has been suggested to suppress postprandial hyperlipidemia and promote negative caloric balance by increasing energy expenditure, due to intestinal physiochemical dynamics that differ from triacylglycerol (TAG).
  • Our study (Study 1) demonstrated that DAG suppressed postprandial increase in TG-rich lipoprotein, very low density lipoprotein (VLDL), and insulin as compared with TAG in young male individuals.
  • Further, our another study using the apolipoprotein C-II deficient subject demonstrated that DAG suppressed postprandial increase in VLDL-cholesterol and remnant-like particle-cholesterol compared with TAG, suggesting that DAG suppress postprandial TG-rich lipoprotein independent of lipoprotein lipase.

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  • (PMID = 20169943.001).
  • [ISSN] 0047-1860
  • [Journal-full-title] Rinsho byori. The Japanese journal of clinical pathology
  • [ISO-abbreviation] Rinsho Byori
  • [Language] jpn
  • [Publication-type] English Abstract; Journal Article; Review
  • [Publication-country] Japan
  • [Chemical-registry-number] 0 / Anti-Obesity Agents; 0 / Cholesterol, VLDL; 0 / Diglycerides; 0 / Insulin; 0 / Lipoproteins, VLDL; 0 / Triglycerides
  • [Number-of-references] 9
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47. Sung MK, Ha CW, Huh WK: A vector system for efficient and economical switching of C-terminal epitope tags in Saccharomyces cerevisiae. Yeast; 2008 Apr;25(4):301-11
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  • [Title] A vector system for efficient and economical switching of C-terminal epitope tags in Saccharomyces cerevisiae.
  • For many purposes, C-terminal tagging is advantageous in that the expression pattern of epitope tag is comparable to that of the authentic protein and the possibility for the tag to affect normal folding of polypeptide chain during translation is minimized.
  • Here, we describe development of a series of plasmids that allow efficient and economical switching of C-terminally tagged epitopes, using just one set of universal oligonucleotide primers.
  • Containing a variety of epitopes (GFP, TAP, GST, Myc, HA and FLAG tag) and Kluyveromyces lactis URA3 gene as a selectable marker, the plasmids can be used to replace any MX6 module-based C-terminal epitope tag with one of the six epitopes.
  • Furthermore, the plasmids also allow additional C-terminal epitope tagging of proteins in yeast cells that already carry MX6 module-based gene deletion or C-terminal epitope tag.

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  • [Copyright] (c) 2008 John Wiley & Sons, Ltd.
  • (PMID = 18350525.001).
  • [ISSN] 1097-0061
  • [Journal-full-title] Yeast (Chichester, England)
  • [ISO-abbreviation] Yeast
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Epitopes; 0 / Saccharomyces cerevisiae Proteins
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48. Mansure JJ, Hallenbeck PC: Desulfovibrio vulgaris Hildenborough HydE and HydG interact with the HydA subunit of the [FeFe] hydrogenase. Biotechnol Lett; 2008 Oct;30(10):1765-9
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  • The hydE, hydF, hydG, hydA, and hydB genes of Desulfovibrio vulgaris Hildenborough were cloned and His-tag pull-down assays were used to study the potential interaction between HydE, HydF, and HydG with the HydA and HydB protein subunits of the D. vulgaris [FeFe] hydrogenase.

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  • (PMID = 18563582.001).
  • [ISSN] 0141-5492
  • [Journal-full-title] Biotechnology letters
  • [ISO-abbreviation] Biotechnol. Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Bacterial Proteins; 0 / Iron-Sulfur Proteins; EC 1.12.- / iron hydrogenase; EC 1.12.7.2 / Hydrogenase
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49. Wang W, Han T, Zhang X, Wu H, Shui Y: Rayleigh wave reflection and scattering calculation by source regeneration method. IEEE Trans Ultrason Ferroelectr Freq Control; 2007 Jul;54(7):1445-53
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  • The analysis and precise calculation of reflection and scattering of Rayleigh wave by electrodes is important for surface acoustic wave (SAW) device models, especially for SAW identification (ID) tag design.

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  • (PMID = 17718334.001).
  • [ISSN] 0885-3010
  • [Journal-full-title] IEEE transactions on ultrasonics, ferroelectrics, and frequency control
  • [ISO-abbreviation] IEEE Trans Ultrason Ferroelectr Freq Control
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
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50. Mayfield SP, Franklin SE: Expression of human antibodies in eukaryotic micro-algae. Vaccine; 2005 Mar 7;23(15):1828-32
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  • Unfortunately, with this clinical success comes a heavy price tag, owing to the inherently high costs of capitalization and production using mammalian cell fermentation.

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  • (PMID = 15734050.001).
  • [ISSN] 0264-410X
  • [Journal-full-title] Vaccine
  • [ISO-abbreviation] Vaccine
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / AI059614-02; United States / NIAID NIH HHS / AI / R01 AI059614; United States / NIAID NIH HHS / AI / R01 AI059614-02; United States / NIGMS NIH HHS / GM / R01GM54659
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.; Review
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Antibodies; 0 / Recombinant Proteins
  • [Number-of-references] 32
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51. Wang W, Qi ZZ, Zhang QW, Wu BC, Zhu ZW, Yang YH, Cui BZ, Dai RX, Qiu YF, Wang ZY, Guo ZB, Shi TX, Wang H, Yang RF, Wang XY: Different strategies for preparation of non-tagged rV270 protein and its efficacy against Yersinia pestis challenge. Biomed Environ Sci; 2010 Oct;23(5):333-40
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  • OBJECTIVE: LcrV is an important component for the development of a subunit vaccine against plague.
  • After Co(2+) affinity chromatography, a purification strategy was developed by cleavage of His tag on column, following Sephacryl S-200HR column filtration chromatography.
  • RESULTS: Removal of His tag by thrombin, enterokinase and factor Xa displayed a yield of 99.5%, 32.4% and 15.3%, respectively.

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  • [Copyright] Copyright © 2010 The Editorial Board of Biomedical and Environmental Sciences. Published by Elsevier B.V. All rights reserved.
  • (PMID = 21112480.001).
  • [ISSN] 0895-3988
  • [Journal-full-title] Biomedical and environmental sciences : BES
  • [ISO-abbreviation] Biomed. Environ. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antibodies, Bacterial; 0 / Antigens, Bacterial; 0 / LcrV protein, Yersinia; 0 / Plague Vaccine; 0 / Pore Forming Cytotoxic Proteins; 0 / Recombinant Fusion Proteins; 0 / Vaccines, Subunit
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52. Aoun M, Michel F, Fouret G, Casas F, Jullien M, Wrutniak-Cabello C, Ramos J, Cristol JP, Coudray C, Carbonneau MA, Feillet-Coudray C: A polyphenol extract modifies quantity but not quality of liver fatty acid content in high-fat-high-sucrose diet-fed rats: possible implication of the sirtuin pathway. Br J Nutr; 2010 Dec;104(12):1760-70
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  • The PPE decreased liver TAG content in HFHS+PPE diet-fed rats in comparison with HFHS diet-fed rats.
  • In conclusion, the PPE partially prevented the accumulation of TAG in the liver by regulating acetyl-CoA carboxylase phosphorylation, a key enzyme in lipid metabolism, probably via sirtuin-1 deacetylase activation.

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  • (PMID = 20673376.001).
  • [ISSN] 1475-2662
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Dietary Fats; 0 / Dietary Sucrose; 0 / Fatty Acids; 0 / Flavonoids; 0 / Phenols; 0 / Plant Extracts; 0 / Polyphenols; 0 / RNA, Messenger; EC 3.5.1.- / Sirtuin 1
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53. Ratnakumar P, Vadez V, Nigam SN, Krishnamurthy L: Assessment of transpiration efficiency in peanut (Arachis hypogaea L.) under drought using a lysimetric system. Plant Biol (Stuttg); 2009 Nov;11 Suppl 1:124-30
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  • The variation in TE was assessed gravimetrically using a long time interval in nine peanut genotypes (Chico, ICGS 44, ICGV 00350, ICGV 86015, ICGV 86031, ICGV 91114, JL 24, TAG 24 and TMV 2) grown in lysimeters under well-watered or drought conditions.
  • A higher TE was found in ICGV 86031 in both well-watered and drought conditions and lower TE was found in TAG-24 under both water regimes.

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  • (PMID = 19778376.001).
  • [ISSN] 1435-8603
  • [Journal-full-title] Plant biology (Stuttgart, Germany)
  • [ISO-abbreviation] Plant Biol (Stuttg)
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Soil
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54. Egert S, Bosy-Westphal A, Seiberl J, Kürbitz C, Settler U, Plachta-Danielzik S, Wagner AE, Frank J, Schrezenmeir J, Rimbach G, Wolffram S, Müller MJ: Quercetin reduces systolic blood pressure and plasma oxidised low-density lipoprotein concentrations in overweight subjects with a high-cardiovascular disease risk phenotype: a double-blinded, placebo-controlled cross-over study. Br J Nutr; 2009 Oct;102(7):1065-74
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  • Quercetin decreased serum HDL-cholesterol concentrations (P < 0.001), while total cholesterol, TAG and the LDL:HDL-cholesterol and TAG:HDL-cholesterol ratios were unaltered.

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  • (PMID = 19402938.001).
  • [ISSN] 1475-2662
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Antioxidants; 0 / Blood Glucose; 0 / Inflammation Mediators; 0 / Lipids; 0 / Lipoproteins, LDL; 0 / oxidized low density lipoprotein; 268B43MJ25 / Uric Acid; 9IKM0I5T1E / Quercetin
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55. Royle PJ, McIntosh GH, Clifton PM: Whey protein isolate and glycomacropeptide decrease weight gain and alter body composition in male Wistar rats. Br J Nutr; 2008 Jul;100(1):88-93
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  • Body composition was assessed, and plasma samples were assayed for TAG, insulin and glucose.
  • Plasma TAG in the WPI+GMP (200 g/kg) group were lower (- 27 %; P < 0.05) than the casein and beef groups.

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  • (PMID = 18062832.001).
  • [ISSN] 1475-2662
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Amino Acids; 0 / Glycopeptides; 0 / Insulin; 0 / Milk Proteins; 0 / Triglycerides; 0 / Whey Proteins
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56. Gil-Campos M, Larqué E, Ramírez-Tortosa MC, Linde J, Villada I, Cañete R, Gil A: Changes in plasma fatty acid composition after intake of a standardised breakfast in prepubertal obese children. Br J Nutr; 2008 Apr;99(4):909-17
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  • [Title] Changes in plasma fatty acid composition after intake of a standardised breakfast in prepubertal obese children.
  • The objective was to assess changes in plasma fatty acid composition in total plasma lipids and plasma lipid fractions in obese prepubertal children compared with those of normal weight and to evaluate changes in postprandial plasma fatty acids during a 3 h period after intake of a standardised breakfast.
  • Except for 18 : 0, 18 : 3n-3, 20 : 4n-6 and n-3 PUFA, all fatty acids in TAG were also elevated in the obese group.
  • Obese prepubertal children show an altered plasma fatty acid profile and concentrations, mainly related to the TAG fatty acid profile, with a lower clearance of fatty acids v. normal-weight prepubertal children.

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  • (PMID = 17903339.001).
  • [ISSN] 0007-1145
  • [Journal-full-title] The British journal of nutrition
  • [ISO-abbreviation] Br. J. Nutr.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Cholesterol Esters; 0 / Fatty Acids; 0 / Fatty Acids, Nonesterified; 0 / Lipids; 0 / Triglycerides
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57. Milani L, Fredriksson M, Syvänen AC: Detection of alternatively spliced transcripts in leukemia cell lines by minisequencing on microarrays. Clin Chem; 2006 Feb;52(2):202-11
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  • METHODS: We devised a new approach for detecting splice variants based on a tag-microarray minisequencing system, originally developed for genotyping single-nucleotide polymorphisms.
  • Size analysis of the PCR products subjected to the tag-array minisequencing system and real-time PCR with exon-junction probes verified the results from the microarray system.

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  • (PMID = 16384885.001).
  • [ISSN] 0009-9147
  • [Journal-full-title] Clinical chemistry
  • [ISO-abbreviation] Clin. Chem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / DNA, Complementary
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58. Brodin P, de Jonge MI, Majlessi L, Leclerc C, Nilges M, Cole ST, Brosch R: Functional analysis of early secreted antigenic target-6, the dominant T-cell antigen of Mycobacterium tuberculosis, reveals key residues involved in secretion, complex formation, virulence, and immunogenicity. J Biol Chem; 2005 Oct 7;280(40):33953-9
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  • Construction of recombinant BCG expressing ESAT-6 with a C-terminal hexahistidine tag allowed us to co-purify ESAT-6 and CFP-10, experimentally confirming their strong interaction both in and outside of the mycobacterial cell.
  • The strain induced potent, antigen-specific T-cell responses and intermediate in vivo growth in mice, suggesting that it remained immunogenic and biologically active despite the tag.

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  • (PMID = 16048998.001).
  • [ISSN] 0021-9258
  • [Journal-full-title] The Journal of biological chemistry
  • [ISO-abbreviation] J. Biol. Chem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antigens, Bacterial; 0 / Bacterial Proteins; 0 / DNA, Bacterial; 0 / ESAT-6 protein, Mycobacterium tuberculosis; 0 / Macromolecular Substances
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59. Kumari R, Jaisankar TJ, Thappa DM: A clinical study of skin changes in pregnancy. Indian J Dermatol Venereol Leprol; 2007 Mar-Apr;73(2):141
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  • [Title] A clinical study of skin changes in pregnancy.
  • BACKGROUND: During pregnancy profound immunologic, metabolic, endocrine and vascular changes occur, that are responsible for the changes of the skin and its appendages, both physiologic and pathologic.
  • AIMS: We undertook a clinical study to find out the frequency and pattern of skin changes in pregnant women.
  • Skin changes grouped into: physiological changes (all cases), specific dermatoses (22 cases) and other dermatoses affected by pregnancy (125 cases).
  • Of the various specific dermatoses of pregnancy, pruritic urticarial papules and plaques of pregnancy (PUPPP) was the most common disorder (14 cases) followed by pruritus gravidarum (5 cases).
  • The most common dermatoses affected by pregnancy were candidal vaginitis (17 cases), acne vulgaris (15 cases), skin tags (15 cases), eczemas (14 cases).
  • CONCLUSION: This study brings into focus various skin changes during pregnancy in south India.
  • [MeSH-major] Pregnancy Complications / epidemiology. Skin Diseases / epidemiology
  • [MeSH-minor] Adolescent. Adult. Female. Humans. India / epidemiology. Pregnancy. Pruritus / epidemiology. Skin Pigmentation / physiology

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  • (PMID = 17458033.001).
  • [ISSN] 0973-3922
  • [Journal-full-title] Indian journal of dermatology, venereology and leprology
  • [ISO-abbreviation] Indian J Dermatol Venereol Leprol
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] India
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60. Maillot F, Garrigue MA, Pinault M, Objois M, Théret V, Lamisse F, Hoinard C, Antoine JM, Lairon D, Couet C: Changes in plasma triacylglycerol concentrations after sequential lunch and dinner in healthy subjects. Diabetes Metab; 2005 Feb;31(1):69-77
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  • OBJECTIVES: The present study examines the kinetic of plasma triacylglycerol (TAG) after sequential ingestion of lunch and dinner as well as the contribution of dietary fat ingested at lunch to subsequent post-dinner TAG composition.
  • Plasma TAG and chylomicron-TAG (CMTAG) levels were measured sequentially after meals.
  • RESULT: Post-dinner plasma TAG peak was delayed as compared to lunch (3 hours vs 1 hour, p=0.002) whereas the magnitude of the postprandial peaks was not significantly different between lunch and dinner (2.4+/-0.3 vs 2.0+/-0.4 mmol/L, p=0.85).
  • A delayed peak of plasma and CM-TAG was observed after dinner as compared to lunch without difference in the magnitude of peaks.
  • CONCLUSION: The contribution of dietary fat ingested at lunch to post-dinner lipemia is confirmed despite the relatively long lasting interval between the 2 meals (7 h) and the absence of any early peak of plasma TAG after dinner.

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  • (PMID = 15803116.001).
  • [ISSN] 1262-3636
  • [Journal-full-title] Diabetes & metabolism
  • [ISO-abbreviation] Diabetes Metab.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] France
  • [Chemical-registry-number] 0 / Dietary Fats; 0 / Olive Oil; 0 / Plant Oils; 0 / Triglycerides; 8001-21-6 / sunflower seed oil
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66. Quintero M, Cabañas ME, Arús C: A possible cellular explanation for the NMR-visible mobile lipid (ML) changes in cultured C6 glioma cells with growth. Biochim Biophys Acta; 2007 Jan;1771(1):31-44
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  • When neutral lipid content is quantified by thin-layer chromatography (TLC) on total lipid extracts of C6 cells, no statistically significant change can be detected (in microg/10(8) cells) with growth or growth arrest in major neutral lipid containing species (triacylglycerol, TAG, diacylglycerol, DAG, cholesteryl esters, ChoEst) except for DAG, which decreased in post-confluent, 7-day cells.
  • A cellular explanation for the observed results is proposed: the TAG-droplet-size-change hypothesis.

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  • (PMID = 17150408.001).
  • [ISSN] 0006-3002
  • [Journal-full-title] Biochimica et biophysica acta
  • [ISO-abbreviation] Biochim. Biophys. Acta
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Fluorescent Dyes; 0 / Intercellular Signaling Peptides and Proteins; 0 / Oxazines; P476F1L81G / nile red
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67. Blomberg Le Ann, Garrett WM, Guillomot M, Miles JR, Sonstegard TS, Van Tassell CP, Zuelke KA: Transcriptome profiling of the tubular porcine conceptus identifies the differential regulation of growth and developmentally associated genes. Mol Reprod Dev; 2006 Dec;73(12):1491-502
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  • Comparing the three libraries at 12,000 tags/library indicated small-amplified RNA-SAGE was a reliable amplification procedure.
  • The unamplified library was increased to 42,415 tags and statistical analyses of tag frequencies with previously generated ovoid and filamentous libraries revealed the differential expression (P < 0.05) of 483 and 364 tags between ovoid:tubular or tubular:filamentous libraries, respectively.

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  • (PMID = 16902953.001).
  • [ISSN] 1040-452X
  • [Journal-full-title] Molecular reproduction and development
  • [ISO-abbreviation] Mol. Reprod. Dev.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article
  • [Publication-country] United States
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68. Schimanski B, Nguyen TN, Günzl A: Highly efficient tandem affinity purification of trypanosome protein complexes based on a novel epitope combination. Eukaryot Cell; 2005 Nov;4(11):1942-50
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  • However, we found that the original method, which is based on the TAP tag, consisting of a duplicate protein A epitope, a tobacco etch virus protease cleavage site, and the calmodulin-binding peptide (CBP), did not yield enough recovery of transcription factor SNAPc (for small nuclear RNA-activating protein complex) from crude trypanosome extracts for protein identification.
  • To overcome this problem, we replaced CBP by the protein C epitope (ProtC) and termed this new epitope combination PTP tag.

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  • (PMID = 16278461.001).
  • [ISSN] 1535-9778
  • [Journal-full-title] Eukaryotic cell
  • [ISO-abbreviation] Eukaryotic Cell
  • [Language] ENG
  • [Grant] United States / NIAID NIH HHS / AI / R01 AI059377; United States / NIAID NIH HHS / AI / AI059377
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Epitopes; 0 / Multiprotein Complexes; 0 / Protozoan Proteins; 0 / Recombinant Fusion Proteins; 0 / Transcription Factor TFIIA; EC 2.7.7.- / RNA Polymerase I
  • [Other-IDs] NLM/ PMC1287860
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69. Matsui H, Sato K, Sakakibara Y: Pair stochastic tree adjoining grammars for aligning and predicting pseudoknot RNA structures. Bioinformatics; 2005 Jun 1;21(11):2611-7
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  • On the other hand, tree adjoining grammars (TAGs), a subclass of context-sensitive grammars, are suitable for modeling pseudoknots.
  • Our goal is to extend PHMMTSs by incorporating TAGs to be able to handle pseudoknots.
  • RESULTS: We propose pair stochastic TAGs (PSTAGs) for aligning and predicting RNA secondary structures including a simple type of pseudoknot which can represent most known pseudoknot structures.
  • First, we extend PHMMTSs defined on alignment of 'trees' to PSTAGs defined on alignment of 'TAG trees' which represent derivation processes of TAGs and are functionally equivalent to derived trees of TAGs.

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  • (PMID = 15784748.001).
  • [ISSN] 1367-4803
  • [Journal-full-title] Bioinformatics (Oxford, England)
  • [ISO-abbreviation] Bioinformatics
  • [Language] eng
  • [Publication-type] Evaluation Studies; Journal Article
  • [Publication-country] England
  • [Chemical-registry-number] 63231-63-0 / RNA
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70. Collén A, Saloheimo M, Bailey M, Penttilä M, Pakula TM: Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag. Biotechnol Bioeng; 2005 Feb 5;89(3):335-44
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  • [Title] Protein production and induction of the unfolded protein response in Trichoderma reesei strain Rut-C30 and its transformant expressing endoglucanase I with a hydrophobic tag.
  • The effect of induction of protein production was studied in bioreactor cultures of T. reesei strain Rut-C30 and its transformant expressing endoglucanase I core domain (EGI, Cel7B) fused with a hydrophobic peptide tag.
  • The tag was previously designed for efficient purification of the fusion protein in aqueous two-phase separation.

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  • [Copyright] 2004 Wiley Periodicals, Inc.
  • (PMID = 15619324.001).
  • [ISSN] 0006-3592
  • [Journal-full-title] Biotechnology and bioengineering
  • [ISO-abbreviation] Biotechnol. Bioeng.
  • [Language] eng
  • [Publication-type] Comparative Study; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / RNA, Messenger; 0 / Recombinant Fusion Proteins; EC 3.2.1.- / Cellulases; EC 3.2.1.4 / Cellulase; EC 3.2.1.4 / endoglucanase EGL1; EC 3.2.1.91 / Cellulose 1,4-beta-Cellobiosidase; IY9XDZ35W2 / Glucose; J2B2A4N98G / Lactose
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71. Broderick P, Carvajal-Carmona L, Pittman AM, Webb E, Howarth K, Rowan A, Lubbe S, Spain S, Sullivan K, Fielding S, Jaeger E, Vijayakrishnan J, Kemp Z, Gorman M, Chandler I, Papaemmanuil E, Penegar S, Wood W, Sellick G, Qureshi M, Teixeira A, Domingo E, Barclay E, Martin L, Sieber O, CORGI Consortium, Kerr D, Gray R, Peto J, Cazier JB, Tomlinson I, Houlston RS: A genome-wide association study shows that common alleles of SMAD7 influence colorectal cancer risk. Nat Genet; 2007 Nov;39(11):1315-7
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  • To identify risk variants for colorectal cancer (CRC), we conducted a genome-wide association study, genotyping 550,163 tag SNPs in 940 individuals with familial colorectal tumor (627 CRC, 313 advanced adenomas) and 965 controls.


72. Abdoun KA, Amin AS, Abdelatif AM: Milk composition of dromedary camels (Camelus dromedarius): nutritional effects and correlation to corresponding blood parameters. Pak J Biol Sci; 2007 Aug 15;10(16):2724-7
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  • However, the significantly (p<0.05) higher lipogenic content (ether extract + crude fiber) of the plant selected during the dry season resulted in significantly (p<0.05) higher serum triacylglycerides concentration and significantly (p<0.05) higher milk fat content compared to that of the green season.

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  • (PMID = 19070090.001).
  • [ISSN] 1028-8880
  • [Journal-full-title] Pakistan journal of biological sciences : PJBS
  • [ISO-abbreviation] Pak. J. Biol. Sci.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Pakistan
  • [Chemical-registry-number] 0 / Blood Glucose; J2B2A4N98G / Lactose; N762921K75 / Nitrogen
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73. Chen D, He N, Zhang M: [Expression of a shrimp Kunitz-type protease inhibitor in Pichia pastoris and activity analysis]. Sheng Wu Gong Cheng Xue Bao; 2008 Mar;24(3):500-3
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  • [Title] [Expression of a shrimp Kunitz-type protease inhibitor in Pichia pastoris and activity analysis].
  • To fully understand its function in the innate immunity of shrimp, the skpi gene was cloned into a modified pPIC9K vector with a 6-His tag and expressed by Pichia pastoris GS115.

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  • (PMID = 18589830.001).
  • [ISSN] 1000-3061
  • [Journal-full-title] Sheng wu gong cheng xue bao = Chinese journal of biotechnology
  • [ISO-abbreviation] Sheng Wu Gong Cheng Xue Bao
  • [Language] chi
  • [Publication-type] English Abstract; Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] China
  • [Chemical-registry-number] 0 / Recombinant Proteins; 0 / Serine Proteinase Inhibitors; 0 / Trypsin Inhibitors; 0 / recombinant Kunitz protease inhibitor; 9087-70-1 / Aprotinin
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74. Hsu FC, Ho TJ, Lai CC, Lin CF, Chen HP: Cloning, sequencing, expression, and single-step purification of the adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU) from Salmonella typhimurium ATCC 19585. Protein Expr Purif; 2005 Jul;42(1):178-81
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  • The bi-functional enzyme, adenosylcobinamide kinase/adenosylcobinamide-phosphate guanylyltransferase (CobU), is involved in the biosynthesis of cobalamin in Salmonella typhimurium, and, therefore, can be used for the in vitro synthesis of analogs of B(12).
  • Here, we describe the cloning, sequencing, and expression of the cobU gene from S. typhimurium ATCC 19585 and, without introducing a purification tag sequence to the N- or C-terminus of the recombinant enzyme, a new single-step purification method based on hydrophobic interaction chromatography.

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  • (PMID = 15939305.001).
  • [ISSN] 1046-5928
  • [Journal-full-title] Protein expression and purification
  • [ISO-abbreviation] Protein Expr. Purif.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Multienzyme Complexes; 0 / Recombinant Proteins; 71822-39-4 / phenyl-sepharose; 9012-36-6 / Sepharose; EC 2.4.2.- / Pentosyltransferases; EC 2.4.2.21 / nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase; EC 2.7.7.- / Nucleotidyltransferases
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75. Pedersen MH, Mølgaard C, Hellgren LI, Lauritzen L: Effects of fish oil supplementation on markers of the metabolic syndrome. J Pediatr; 2010 Sep;157(3):395-400, 400.e1
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  • STUDY DESIGN: A total of 78 boys age 13-15 years with a mean body fat percentage of 30%+/-9% were randomly assigned to consume fish oil (providing 1.5 g of n-3 long-chain polyunsaturated fatty acid/day) or vegetable oil (control) for 16 weeks.
  • Plasma triacylglycerol (TAG) concentration and insulin sensitivity were unaffected by either of the treatments.
  • No association was seen between RBC EPA and plasma TAG concentration or insulin sensitivity.

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  • [Copyright] Copyright (c) 2010 Mosby, Inc. All rights reserved.
  • (PMID = 20472253.001).
  • [ISSN] 1097-6833
  • [Journal-full-title] The Journal of pediatrics
  • [ISO-abbreviation] J. Pediatr.
  • [Language] eng
  • [Databank-accession-numbers] ClinicalTrials.gov/ NCT00929552
  • [Publication-type] Journal Article; Randomized Controlled Trial
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Biomarkers; 0 / Fish Oils
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76. Yanga Y, Tiana Z, Teng D, Zhang J, Wang J, Wang J: High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression. J Biotechnol; 2009 Feb 23;139(4):326-31
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  • [Title] High-level production of a candidacidal peptide lactoferrampin in Escherichia coli by fusion expression.
  • The soluble fusion protein was easily purified to near homogeneity by affinity chromatography using hexahistidine tag.
  • An unknown site in the Trx-tag fusion protein, which can be cleaved by factor Xa to produce approximately 10 kDa protein, was found.

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  • (PMID = 19297728.001).
  • [ISSN] 1873-4863
  • [Journal-full-title] Journal of biotechnology
  • [ISO-abbreviation] J. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / Antifungal Agents; 0 / LFampin 265-284; 0 / Peptide Fragments; 0 / Recombinant Fusion Proteins; EC 3.4.21.- / Lactoferrin
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77. Chang HW, Chuang LY, Chang YJ, Cheng YH, Hung YC, Chen HC, Yang CH: LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs. BMC Genet; 2009;10:26
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  • [Title] LD2SNPing: linkage disequilibrium plotter and RFLP enzyme mining for tag SNPs.
  • The software provides SNP ID- and gene-centric online retrievals for SNP information and tag SNP selection from dbSNP/NCBI and HapMap, respectively.
  • Restriction fragment length polymorphism (RFLP) enzyme information for SNP genotype is available to all SNP IDs and tag SNPs.
  • An LD statistics section provides D, D', r2, deltaQ, rho, and the P values of the Hardy-Weinberg Equilibrium for each SNP marker, and Chi-square and likelihood-ratio tests for the pair-wise association of two SNPs in LD calculation.

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  • (PMID = 19500380.001).
  • [ISSN] 1471-2156
  • [Journal-full-title] BMC genetics
  • [ISO-abbreviation] BMC Genet.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Other-IDs] NLM/ PMC2709117
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78. Dillavou ED, Makaroun MS: Predictors of morbidity and mortality with endovascular and open thoracic aneurysm repair. J Vasc Surg; 2008 Nov;48(5):1114-9; discussion 1119-20
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  • METHODS: The database of the GORE TAG (W.L.
  • Gore, Flagstaff, Ariz) Pivotal Trial comparing the TAG endograft to open repair was interrogated.
  • Univariate (UVA) and multivariate analyses (MVA) of demographic, clinical, anatomic, and procedural variables were conducted to discover possible predictors of serious adverse events for the whole group and for the TAG and open cohort groups separately.
  • RESULTS: A total of 140 TAG and 94 open descending thoracic aneurysm (DTA) patients were analyzed, consisting of 128 men and 106 women.
  • Perioperative deaths were 9/94 for open surgery and 3/140 for TAG patients, with 10/12 (7 open, 3 TAG) deaths occurring in men.
  • Analysis of a combined morbidity/mortality endpoint (stroke/paralysis/MI/death) showed elevated creatinine predicted these events for the whole group.
  • Vascular complications were more frequent in the TAG (19%) than in open DTA patients (9%) (P = .038).
  • Women were noted to have longer procedure times for both TAG and open repairs.

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  • (PMID = 18771887.001).
  • [ISSN] 1097-6809
  • [Journal-full-title] Journal of vascular surgery
  • [ISO-abbreviation] J. Vasc. Surg.
  • [Language] eng
  • [Publication-type] Clinical Trial, Phase II; Comparative Study; Controlled Clinical Trial; Journal Article; Multicenter Study
  • [Publication-country] United States
  • [Chemical-registry-number] AYI8EX34EU / Creatinine
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79. Zhang Y, Wang D, Sadée W: Calmodulin interaction with peptides from G-protein coupled receptors measured with S-Tag labeling. Biochem Biophys Res Commun; 2005 Jul 29;333(2):390-5
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  • [Title] Calmodulin interaction with peptides from G-protein coupled receptors measured with S-Tag labeling.
  • We have developed a quantitative assay of calmodulin (CaM) binding to S-Tag labeled peptides derived from G-protein coupled receptor (GPCR) sequences.
  • The use of S-Tag labeling can serve for rapid screening of putative CaM-binding domains in GPCRs.

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  • (PMID = 15950946.001).
  • [ISSN] 0006-291X
  • [Journal-full-title] Biochemical and biophysical research communications
  • [ISO-abbreviation] Biochem. Biophys. Res. Commun.
  • [Language] eng
  • [Grant] United States / NIDA NIH HHS / DA / DA 04166
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Calmodulin; 0 / Peptide Fragments; 0 / Peptides; 0 / Receptors, G-Protein-Coupled; 0 / Recombinant Fusion Proteins; 0 / ribonuclease S-peptide; EC 3.1.- / Ribonucleases
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80. Haab BB: Multiplexed protein analysis using antibody microarrays and label-based detection. Methods Mol Med; 2005;114:183-94
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • Two related detection methods are described in which the proteins of a sample to be analyzed are labeled with a tag to allow detection after capture on antibody microarrays.

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  • (PMID = 16156104.001).
  • [ISSN] 1543-1894
  • [Journal-full-title] Methods in molecular medicine
  • [ISO-abbreviation] Methods Mol. Med.
  • [Language] eng
  • [Grant] United States / NCI NIH HHS / CA / U01 CA84986
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Antibodies; 0 / Proteins
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81. Duan W, Gabril MY, Moussa M, Chan FL, Sakai H, Fong G, Xuan JW: Knockin of SV40 Tag oncogene in a mouse adenocarcinoma of the prostate model demonstrates advantageous features over the transgenic model. Oncogene; 2005 Feb 24;24(9):1510-24
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  • [Title] Knockin of SV40 Tag oncogene in a mouse adenocarcinoma of the prostate model demonstrates advantageous features over the transgenic model.
  • Since there is no naturally occurring prostate cancer in the mouse, preclinical studies stipulate for the establishment of a genetically manipulated mouse CaP model with features close to the human situation.
  • In view of the limitations of transgenic technique-derived CaP models, herein we report the first application of knockin technology to establish a new mouse adenocarcinoma prostate model (PSP-KIMAP) by targeting of SV40 Tag to a prostate tissue-specific gene, PSP94 (prostate secretory protein of 94 amino acids).
  • The differences between the two CaP models were attributed to the introduction of a single endogenous knockin mutation, resulting in a CaP model self-regulated and controlled by a prostate gene promoter/enhancer of PSP94.


82. Shirts BH, Wood J, Yolken RH, Nimgaonkar VL: Association study of IL10, IL1beta, and IL1RN and schizophrenia using tag SNPs from a comprehensive database: suggestive association with rs16944 at IL1beta. Schizophr Res; 2006 Dec;88(1-3):235-44
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  • [Title] Association study of IL10, IL1beta, and IL1RN and schizophrenia using tag SNPs from a comprehensive database: suggestive association with rs16944 at IL1beta.
  • We used comprehensive sequencing data from the Seattle SNPs database to select tag SNPs that represent all common polymorphisms in the Caucasian population at these loci.
  • Associations with 28 tag SNPs were evaluated in 478 cases and 501 unscreened control individuals, while accounting for population sub-structure using the genomic control method.
  • [MeSH-minor] Adult. Alleles. Databases, Genetic. Expressed Sequence Tags. Female. Genotype. Haplotypes / genetics. Humans. Male. Meta-Analysis as Topic

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  • (PMID = 16905295.001).
  • [ISSN] 0920-9964
  • [Journal-full-title] Schizophrenia research
  • [ISO-abbreviation] Schizophr. Res.
  • [Language] eng
  • [Grant] United States / NIMH NIH HHS / MH / MH074265; United States / NIMH NIH HHS / MH / MH56242
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] Netherlands
  • [Chemical-registry-number] 0 / IL10 protein, human; 0 / IL1B protein, human; 0 / IL1RN protein, human; 0 / Interleukin 1 Receptor Antagonist Protein; 0 / Interleukin-1beta; 130068-27-8 / Interleukin-10
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83. Krupoves A, Seidman EG, Mack D, Israel D, Morgan K, Lambrette P, Costea I, Deslandres C, Grimard G, Law L, Levy E, Amre DK: Associations between ABCB1/MDR1 gene polymorphisms and Crohn's disease: a gene-wide study in a pediatric population. Inflamm Bowel Dis; 2009 Jun;15(6):900-8
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  • Thirteen tag-SNPs and the C3435T variant in the MDR1 gene were genotyped.
  • Single-SNP allelic, genotype as well as gene-wide haplotype associations with CD and its phenotypes at diagnosis were assessed.
  • Specific haplotypes comprising of the tag-SNPs were significantly associated with either colonic or noninflammatory CD.

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  • (PMID = 19107781.001).
  • [ISSN] 1536-4844
  • [Journal-full-title] Inflammatory bowel diseases
  • [ISO-abbreviation] Inflamm. Bowel Dis.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / ABCB1 protein, human; 0 / P-Glycoprotein; 0 / P-Glycoproteins
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84. Mort M, Ivanov D, Cooper DN, Chuzhanova NA: A meta-analysis of nonsense mutations causing human genetic disease. Hum Mutat; 2008 Aug;29(8):1037-47
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  • Pathological nonsense mutations resulting in TGA (38.5%), TAG (40.4%), and TAA (21.1%) occur in different proportions to naturally occurring stop codons.
  • Of the 23 different nucleotide substitutions giving rise to nonsense mutations, the most frequent are CGA --> TGA (21%; resulting from methylation-mediated deamination) and CAG --> TAG (19%).
  • Consistent with the operation of a clinical selection bias, genes exhibiting an excess of nonsense mutations are also likely to display an excess of frameshift mutations.

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  • (PMID = 18454449.001).
  • [ISSN] 1098-1004
  • [Journal-full-title] Human mutation
  • [ISO-abbreviation] Hum. Mutat.
  • [Language] eng
  • [Grant] United Kingdom / Medical Research Council / / G0800509
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Codon; 0 / Codon, Nonsense
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85. Qi HG, Liu X, Zhang GF: Characterization of 12 single nucleotide polymorphisms (SNPs) in Pacific abalone, Haliotis discus hannai. Mol Ecol Resour; 2008 Sep;8(5):974-6
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  • We report here for the first time 12 polymorphic single nucleotide polymorphisms (SNPs) in a commercially important gastropod, Pacific abalone (Haliotis discus hannai) that were identified by searching expressed sequence tag database.

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  • [Copyright] © 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Ltd.
  • (PMID = 21585945.001).
  • [ISSN] 1755-098X
  • [Journal-full-title] Molecular ecology resources
  • [ISO-abbreviation] Mol Ecol Resour
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] England
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86. Adler-Wailes DC, Guiney EL, Wolins NE, Yanovski JA: Long-term ritonavir exposure increases fatty acid and glycerol recycling in 3T3-L1 adipocytes as compensatory mechanisms for increased triacylglycerol hydrolysis. Endocrinology; 2010 May;151(5):2097-105
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  • To determine whether ritonavir-treated adipocytes increased glycerol backbone synthesis for FA reesterification, we measured labeled glycerol and pyruvate incorporation into triacylglycerol (TAG).
  • Ritonavir-treated cells had increased FA efflux, uptake, and incorporation into TAG (all P < 0.01).
  • Ritonavir increased FA efflux without consistently increasing glycerol release or changing TAG mass, suggesting increased partial TAG hydrolysis.
  • Ritonavir increased glycerol (P < 0.01) but not pyruvate (P = 0.41), utilization for TAG backbone synthesis.
  • Consistent with this substrate utilization, glycerol kinase transcript (required for glycerol incorporation into TAG backbone) was up-regulated (P < 0.01), whereas phosphoenolpyruvate carboxykinase transcript (required for pyruvate utilization) was down-regulated (P < 0.001).
  • In 3T3-L1 adipocytes, long-term ritonavir exposure perturbs FA metabolism by increasing ATGL-mediated partial TAG hydrolysis, thus increasing FA efflux, and leads to compensatory increases in FA reesterification with glycerol and acylglycerols.

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  • (PMID = 20228169.001).
  • [ISSN] 1945-7170
  • [Journal-full-title] Endocrinology
  • [ISO-abbreviation] Endocrinology
  • [Language] ENG
  • [Grant] United States / NICHD NIH HHS / HD / Z01 HD000641; United States / NICHD NIH HHS / HD / Z01-HD-00641
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Adrenergic beta-Agonists; 0 / Enzyme Inhibitors; 0 / Fatty Acids; 0 / HIV Protease Inhibitors; 0 / Triazenes; 0 / Triglycerides; 76896-80-5 / triacsin C; EC 3.1.1.3 / Lipase; L628TT009W / Isoproterenol; O3J8G9O825 / Ritonavir; PDC6A3C0OX / Glycerol
  • [Other-IDs] NLM/ PMC2869264
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87. Wistow G, Peterson K, Gao J, Buchoff P, Jaworski C, Bowes-Rickman C, Ebright JN, Hauser MA, Hoover D: NEIBank: genomics and bioinformatics resources for vision research. Mol Vis; 2008 Jul 18;14:1327-37
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  • It includes expressed sequence tag (EST) data and sequence-verified cDNA clones for multiple eye tissues of several species, web-based access to human eye-specific SAGE data through EyeSAGE, and comprehensive, annotated databases of known human eye disease genes and candidate disease gene loci.

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  • (PMID = 18648525.001).
  • [ISSN] 1090-0535
  • [Journal-full-title] Molecular vision
  • [ISO-abbreviation] Mol. Vis.
  • [Language] ENG
  • [Grant] United States / NEI NIH HHS / EY / P30EY0054722; United States / NEI NIH HHS / EY / P30 EY005722; United States / NEI NIH HHS / EY / R01 EY11286; United States / NEI NIH HHS / EY / R01 EY011286; United States / NEI NIH HHS / EY / P30 EY005722-22; United States / NEI NIH HHS / EY / R01 EY13315; United States / NEI NIH HHS / EY / R01 EY013315; United States / NEI NIH HHS / EY / EY005722-22
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Other-IDs] NLM/ PMC2480482
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88. Murata J, Bienzle D, Brandle JE, Sensen CW, De Luca V: Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus). FEBS Lett; 2006 Aug 07;580(18):4501-7
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  • [Title] Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus).
  • In spite of its importance, no expressed sequence tag (EST) analysis of this plant has been reported.
  • [MeSH-major] Catharanthus / genetics. Expressed Sequence Tags

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  • (PMID = 16870181.001).
  • [ISSN] 0014-5793
  • [Journal-full-title] FEBS letters
  • [ISO-abbreviation] FEBS Lett.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / ATP-Binding Cassette Transporters; 0 / Indole Alkaloids; 0 / Transcription Factors; 9035-51-2 / Cytochrome P-450 Enzyme System; EC 2.1.1.- / Methyltransferases; EC 2.4.1.- / Glucosyltransferases
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89. Foster JM, Zhang Y, Kumar S, Carlow CK: Mining nematode genome data for novel drug targets. Trends Parasitol; 2005 Mar;21(3):101-4
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  • Expressed sequence tag projects have currently produced over 400 000 partial gene sequences from more than 30 nematode species and the full genomic sequences of selected nematodes are being determined.
  • [MeSH-major] Expressed Sequence Tags. Genes, Helminth. Genome. Nematoda / genetics

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  • (PMID = 15734654.001).
  • [ISSN] 1471-4922
  • [Journal-full-title] Trends in parasitology
  • [ISO-abbreviation] Trends Parasitol.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Number-of-references] 30
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90. Hirano Y, Hossain MM, Takeda K, Tokuda H, Miki K: Purification, crystallization and preliminary X-ray crystallographic analysis of the outer membrane lipoprotein NlpE from Escherichia coli. Acta Crystallogr Sect F Struct Biol Cryst Commun; 2006 Dec 1;62(Pt 12):1227-30
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  • The nonlipidated Cys1Ala mutant of NlpE with a C-terminal His tag from Escherichia coli was constructed, overexpressed and purified.

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  • (PMID = 17142903.001).
  • [ISSN] 1744-3091
  • [Journal-full-title] Acta crystallographica. Section F, Structural biology and crystallization communications
  • [ISO-abbreviation] Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Bacterial Outer Membrane Proteins; 0 / Escherichia coli Proteins; 0 / Lipoproteins; 0 / NlpE protein, E coli
  • [Other-IDs] NLM/ PMC2225377
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91. Mu H, Porsgaard T: The metabolism of structured triacylglycerols. Prog Lipid Res; 2005 Nov;44(6):430-48
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  • The triacylglycerol (TAG) structure in addition to the overall fatty acid profile is of importance when considering the nutritional effect of a dietary fat.
  • This review aims at summarizing our current knowledge of the digestion, absorption, uptake, and transport of structured TAGs, with particular emphasis on the following aspects: gastric emptying, specificity of pancreatic lipase, lymphatic transport and clearance of chylomicrons, effects of lipid structure on tissue lipid compositions and the fecal loss of fats.
  • So an overview will be provided for how the structure and fatty acid composition of TAGs affect their absorption and the distribution of the fatty acids in the body following digestion and absorption.

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  • (PMID = 16269186.001).
  • [ISSN] 0163-7827
  • [Journal-full-title] Progress in lipid research
  • [ISO-abbreviation] Prog. Lipid Res.
  • [Language] eng
  • [Publication-type] Journal Article; Review
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Chylomicrons; 0 / Dietary Fats; 0 / Fatty Acids, Nonesterified; 0 / Triglycerides; EC 3.1.1.3 / Lipase
  • [Number-of-references] 120
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92. Katulanda P, Jayawardena MA, Sheriff MH, Matthews DR: The distance between the lower edge of the xiphisternum and the center of the umbilicus as an indicator of abdominal obesity and cardiovascular disease risk. Obes Facts; 2010 Jun;3(3):201-4
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  • XUD, BMI, waist circumference (WC), waist-to-hip ratio (WHR), systolic and diastolic blood pressure were measured and fasting venous blood samples were collected to determine glucose, total cholesterol, low density lipoprotein (LDL) cholesterol, high density lipoprotein cholesterol and triacylglyceride levels.

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  • [Copyright] Copyright (c) 2010 S. Karger AG, Basel.
  • (PMID = 20616610.001).
  • [ISSN] 1662-4025
  • [Journal-full-title] Obesity facts
  • [ISO-abbreviation] Obes Facts
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] Switzerland
  • [Chemical-registry-number] 0 / Blood Glucose; 0 / Cholesterol, HDL; 0 / Cholesterol, LDL; 0 / Triglycerides
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93. McCormick LC, Slater GW: Molecular deformation and free-solution electrophoresis of DNA-uncharged polymer conjugates at high field strengths: theoretical predictions Part 2: Stretching. Electrophoresis; 2007 Nov;28(21):3837-44
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  • Normally it is possible to separate DNA based on size in free solution; however, not end-labeled free-solution electrophoresis (ELFSE) uses a neutral drag-tag molecule to make it possible.
  • In experiments to date, the drag-tag and DNA together form a random coil conformation; while with future generation drag-tags and high fields, deformation of this conformation may occur.
  • We show that deformation may dramatically improve the capabilities of ELFSE, especially when both the DNA and a polymeric drag-tag fully stretch; however, reaching these regimes will require extremely high field intensities, something that only microchip technologies may be able to achieve.

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  • (PMID = 17922521.001).
  • [ISSN] 0173-0835
  • [Journal-full-title] Electrophoresis
  • [ISO-abbreviation] Electrophoresis
  • [Language] eng
  • [Grant] United States / NHGRI NIH HHS / HG / R01 HG002918-01
  • [Publication-type] Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Biopolymers; 0 / Polymers; 0 / Solutions; 9007-49-2 / DNA
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94. Bhattacharya P, Pandey G, Srivastava P, Mukherjee KJ: Combined effect of protein fusion and signal sequence greatly enhances the production of recombinant human GM-CSF in Escherichia coli. Mol Biotechnol; 2005 Jun;30(2):103-16
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  • To overcome this problem we attached a signal sequence, as well as an amino-terminal His-tag fusion to the GM-CSF gene.
  • When a larger fusion partner, such as the Maltose-binding protein (MBP-tag), was used the expression levels increased further to 69.5 microg/mL, which along with the MBP-tag represented approx 12% of the total cellular protein.

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  • (PMID = 15920280.001).
  • [ISSN] 1073-6085
  • [Journal-full-title] Molecular biotechnology
  • [ISO-abbreviation] Mol. Biotechnol.
  • [Language] eng
  • [Publication-type] Journal Article
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Carrier Proteins; 0 / Codon; 0 / Maltose-Binding Proteins; 0 / Protein Sorting Signals; 0 / RNA, Messenger; 0 / Recombinant Fusion Proteins; 0 / Recombinant Proteins; 83869-56-1 / Granulocyte-Macrophage Colony-Stimulating Factor
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95. Richardson AJ, Islam FM, Guymer RH, Cain M, Baird PN: A tag-single nucleotide polymorphisms approach to the vascular endothelial growth factor-A gene in age-related macular degeneration. Mol Vis; 2007;13:2148-52
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  • [Title] A tag-single nucleotide polymorphisms approach to the vascular endothelial growth factor-A gene in age-related macular degeneration.
  • We employed a tag-single nucleotide polymorphisms (tSNP) approach to investigate this gene using a series of seven tSNPs that encompassed the coding region of the VEGF gene as well as its promoter.

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  • (PMID = 18079689.001).
  • [ISSN] 1090-0535
  • [Journal-full-title] Molecular vision
  • [ISO-abbreviation] Mol. Vis.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
  • [Chemical-registry-number] 0 / Vascular Endothelial Growth Factor A
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96. Ujita M, Nagayama H, Kanie S, Koike S, Ikeyama Y, Ozaki T, Okumura H: Carbohydrate binding specificity of recombinant human macrophage beta-glucan receptor dectin-1. Biosci Biotechnol Biochem; 2009 Jan;73(1):237-40
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  • Human macrophage dectin-1, a type II transmembrane beta-glucan receptor, was expressed as a fusion protein with an N-terminal hexahistidine tag and glutathione S-transferase in an Escherichia coli cell-free translation system, and assayed for binding specificity.

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  • (PMID = 19129647.001).
  • [ISSN] 1347-6947
  • [Journal-full-title] Bioscience, biotechnology, and biochemistry
  • [ISO-abbreviation] Biosci. Biotechnol. Biochem.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Japan
  • [Chemical-registry-number] 0 / Carbohydrates; 0 / Lectins, C-Type; 0 / Membrane Proteins; 0 / Nerve Tissue Proteins; 0 / Receptors, Immunologic; 0 / Recombinant Fusion Proteins; 0 / beta-Glucans; 0 / beta-glucan receptor; 0 / dectin 1; EC 2.5.1.18 / Glutathione Transferase
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97. Reed DG, Nopo-Olazabal LH, Funk V, Woffenden BJ, Reidy MJ, Dolan MC, Cramer CL, Medina-Bolivar F: Expression of functional hexahistidine-tagged ricin B in tobacco. Plant Cell Rep; 2005 Apr;24(1):15-24
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  • [Source] The source of this record is MEDLINE®, a database of the U.S. National Library of Medicine.
  • RTB was engineered with an N-terminal hexahistidine tag (His-RTB), which may affect protein stability.

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  • (PMID = 15599751.001).
  • [ISSN] 0721-7714
  • [Journal-full-title] Plant cell reports
  • [ISO-abbreviation] Plant Cell Rep.
  • [Language] eng
  • [Grant] United States / NIAID NIH HHS / AI / P01 AI-44962
  • [Publication-type] Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / His-His-His-His-His-His; 0 / Oligopeptides; 0 / Recombinant Proteins; 4QD397987E / Histidine; 9009-86-3 / Ricin
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98. Xu Z, Yang W, Shi N, Gao Y, Teng M, Niu L: Cloning, purification, crystallization and preliminary X-ray crystallographic analysis of SET/TAF-Iß δN from Homo sapiens. Acta Crystallogr Sect F Struct Biol Cryst Commun; 2010 Aug 1;66(Pt 8):926-8
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  • A truncated SET/TAF-Iß ΔN protein that lacked the first 22 residues of the N-terminus but contained the C-terminal acidic domain and an additional His6 tag at the C-terminus was overexpressed in Escherichia coli and crystallized by the hanging-drop vapour-diffusion method using sodium acetate as precipitant at 283 K.

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  • (PMID = 20693670.001).
  • [ISSN] 1744-3091
  • [Journal-full-title] Acta crystallographica. Section F, Structural biology and crystallization communications
  • [ISO-abbreviation] Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] England
  • [Chemical-registry-number] 0 / Histone Chaperones; 0 / SET protein, human; 0 / Transcription Factors
  • [Other-IDs] NLM/ PMC2917293
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99. Hausdorf B, Helmkampf M, Meyer A, Witek A, Herlyn H, Bruchhaus I, Hankeln T, Struck TH, Lieb B: Spiralian phylogenomics supports the resurrection of Bryozoa comprising Ectoprocta and Entoprocta. Mol Biol Evol; 2007 Dec;24(12):2723-9
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  • Phylogenetic analyses based on 79 ribosomal proteins of 38 metazoans, partly derived from 6 new expressed sequence tag projects for Ectoprocta, Entoprocta, Sipuncula, Annelida, and Acanthocephala, indicate the monophyly of Bryozoa comprising Ectoprocta and Entoprocta, 2 taxa that have been separated for more than a century based on seemingly profound morphological differences.

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  • (PMID = 17921486.001).
  • [ISSN] 0737-4038
  • [Journal-full-title] Molecular biology and evolution
  • [ISO-abbreviation] Mol. Biol. Evol.
  • [Language] eng
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] United States
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100. Ozawa K, Harashina T, Yatsunami R, Nakamura S: Gene cloning, expression and partial characterization of cell division protein FtsZ1 from extremely halophilic archaeon Haloarcula japonica strain TR-1. Extremophiles; 2005 Aug;9(4):281-8
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  • The recombinant FtsZ1 was produced as a fusion with hexahistidine-tag in Ha. japonica host cells and purified.

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  • Hazardous Substances Data Bank. (L)-HISTIDINE .
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  • Hazardous Substances Data Bank. SODIUM CHLORIDE .
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  • (PMID = 15844012.001).
  • [ISSN] 1431-0651
  • [Journal-full-title] Extremophiles : life under extreme conditions
  • [ISO-abbreviation] Extremophiles
  • [Language] eng
  • [Databank-accession-numbers] GENBANK/ AB196145
  • [Publication-type] Journal Article; Research Support, Non-U.S. Gov't
  • [Publication-country] Germany
  • [Chemical-registry-number] 0 / Archaeal Proteins; 0 / FtsZ protein, Methanococcus jannaschii; 451W47IQ8X / Sodium Chloride; 4QD397987E / Histidine; 660YQ98I10 / Potassium Chloride; EC 3.6.1.- / GTP Phosphohydrolases
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